Polyphenol bioaccessibility and antioxidant properties of in vitro digested spray-dried thermally-treated skimmed goat milk enriched with pollen
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2021
Authors
Kostić, Aleksandar Ž.Milinčić, Danijel D.
Stanisavljević, Nemanja S.
Gašić, Uroš M.
Lević, Steva
Kojić, Milan O.
Tešić, Živoslav Lj.
Nedović, Viktor
Barać, Miroljub B.
Pešić, Mirjana B.
Article (Published version)
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The aim of research was to determine polyphenols bioaccessibility and antioxidant properties of thermally-treated skimmed goat milk enriched with sunflower bee-collected pollen through in vitro digestion. HPLC analysis confirmed that pollen-enriched milk contained flavonols as the main phenolic fraction (80.7–76.2%) followed by phenolic acids (14.2–17.4%). Among individual compounds quercetin-3-O-glucoside (155.1–197.2 μg/L) and p-coumaric acid (29.5–30.7 μg/L) were the main quantified flavonols and phenolic acids, respectively. After digestion of milk/pollen sample, total polyphenols recovery was 30.71% with higher phenolic acids recovery (40.1%) compared to flavonols (28.3%) indicating strong interactions between caprine milk casein micelles and pollen polyphenols. Applied antioxidant assays (phosphomolybdenum, ABTS•+scavenging activity and ferrous-ion-chelating capacity) have confirmed complexity of prepared product- it had high ability to quench ABTS•+ radicals and to form chelatin...g complexes with Fe2+ ions. Digestion provoked 20% reduction in total antioxidant capacity compared to the initial sample. TTSG milk/pollen powder could be good functional ingredient.
Keywords:
Antioxidant properties / Goat milk/pollen powder / In vitro polyphenol bioaccessibility / Monofloral sunflower bee-collected pollenSource:
Food Chemistry, 2021, 351, 129310-Publisher:
- Elsevier Ltd
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200042 (University of Belgrade, Institute of Molecular Genetics and Genetic Engineering) (RS-MESTD-inst-2020-200042)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-MESTD-inst-2020-200007)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200116 (University of Belgrade, Faculty of Agriculture) (RS-MESTD-inst-2020-200116)
DOI: 10.1016/j.foodchem.2021.129310
ISSN: 0308-8146
WoS: 000632300600001
Scopus: 2-s2.0-85101327994
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Poljoprivredni fakultetTY - JOUR AU - Kostić, Aleksandar Ž. AU - Milinčić, Danijel D. AU - Stanisavljević, Nemanja S. AU - Gašić, Uroš M. AU - Lević, Steva AU - Kojić, Milan O. AU - Tešić, Živoslav Lj. AU - Nedović, Viktor AU - Barać, Miroljub B. AU - Pešić, Mirjana B. PY - 2021 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/5814 AB - The aim of research was to determine polyphenols bioaccessibility and antioxidant properties of thermally-treated skimmed goat milk enriched with sunflower bee-collected pollen through in vitro digestion. HPLC analysis confirmed that pollen-enriched milk contained flavonols as the main phenolic fraction (80.7–76.2%) followed by phenolic acids (14.2–17.4%). Among individual compounds quercetin-3-O-glucoside (155.1–197.2 μg/L) and p-coumaric acid (29.5–30.7 μg/L) were the main quantified flavonols and phenolic acids, respectively. After digestion of milk/pollen sample, total polyphenols recovery was 30.71% with higher phenolic acids recovery (40.1%) compared to flavonols (28.3%) indicating strong interactions between caprine milk casein micelles and pollen polyphenols. Applied antioxidant assays (phosphomolybdenum, ABTS•+scavenging activity and ferrous-ion-chelating capacity) have confirmed complexity of prepared product- it had high ability to quench ABTS•+ radicals and to form chelating complexes with Fe2+ ions. Digestion provoked 20% reduction in total antioxidant capacity compared to the initial sample. TTSG milk/pollen powder could be good functional ingredient. PB - Elsevier Ltd T2 - Food Chemistry T1 - Polyphenol bioaccessibility and antioxidant properties of in vitro digested spray-dried thermally-treated skimmed goat milk enriched with pollen SP - 129310 VL - 351 DO - 10.1016/j.foodchem.2021.129310 ER -
@article{ author = "Kostić, Aleksandar Ž. and Milinčić, Danijel D. and Stanisavljević, Nemanja S. and Gašić, Uroš M. and Lević, Steva and Kojić, Milan O. and Tešić, Živoslav Lj. and Nedović, Viktor and Barać, Miroljub B. and Pešić, Mirjana B.", year = "2021", abstract = "The aim of research was to determine polyphenols bioaccessibility and antioxidant properties of thermally-treated skimmed goat milk enriched with sunflower bee-collected pollen through in vitro digestion. HPLC analysis confirmed that pollen-enriched milk contained flavonols as the main phenolic fraction (80.7–76.2%) followed by phenolic acids (14.2–17.4%). Among individual compounds quercetin-3-O-glucoside (155.1–197.2 μg/L) and p-coumaric acid (29.5–30.7 μg/L) were the main quantified flavonols and phenolic acids, respectively. After digestion of milk/pollen sample, total polyphenols recovery was 30.71% with higher phenolic acids recovery (40.1%) compared to flavonols (28.3%) indicating strong interactions between caprine milk casein micelles and pollen polyphenols. Applied antioxidant assays (phosphomolybdenum, ABTS•+scavenging activity and ferrous-ion-chelating capacity) have confirmed complexity of prepared product- it had high ability to quench ABTS•+ radicals and to form chelating complexes with Fe2+ ions. Digestion provoked 20% reduction in total antioxidant capacity compared to the initial sample. TTSG milk/pollen powder could be good functional ingredient.", publisher = "Elsevier Ltd", journal = "Food Chemistry", title = "Polyphenol bioaccessibility and antioxidant properties of in vitro digested spray-dried thermally-treated skimmed goat milk enriched with pollen", pages = "129310", volume = "351", doi = "10.1016/j.foodchem.2021.129310" }
Kostić, A. Ž., Milinčić, D. D., Stanisavljević, N. S., Gašić, U. M., Lević, S., Kojić, M. O., Tešić, Ž. Lj., Nedović, V., Barać, M. B.,& Pešić, M. B.. (2021). Polyphenol bioaccessibility and antioxidant properties of in vitro digested spray-dried thermally-treated skimmed goat milk enriched with pollen. in Food Chemistry Elsevier Ltd., 351, 129310. https://doi.org/10.1016/j.foodchem.2021.129310
Kostić AŽ, Milinčić DD, Stanisavljević NS, Gašić UM, Lević S, Kojić MO, Tešić ŽL, Nedović V, Barać MB, Pešić MB. Polyphenol bioaccessibility and antioxidant properties of in vitro digested spray-dried thermally-treated skimmed goat milk enriched with pollen. in Food Chemistry. 2021;351:129310. doi:10.1016/j.foodchem.2021.129310 .
Kostić, Aleksandar Ž., Milinčić, Danijel D., Stanisavljević, Nemanja S., Gašić, Uroš M., Lević, Steva, Kojić, Milan O., Tešić, Živoslav Lj., Nedović, Viktor, Barać, Miroljub B., Pešić, Mirjana B., "Polyphenol bioaccessibility and antioxidant properties of in vitro digested spray-dried thermally-treated skimmed goat milk enriched with pollen" in Food Chemistry, 351 (2021):129310, https://doi.org/10.1016/j.foodchem.2021.129310 . .