Matrix resistance stress reduction-prerequisite for achieving higher concentration of immobilized cells
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Pajić-Lijaković, IvanaMilivojević, M.
Lević, Steva
Trifković, Kata
Balanc, Bojana
Nedović, Viktor
Dajić-Stevanović, Zora
Radošević, Radenko
Bugarski, Branko
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Some of the main rheological properties that Ca-alginate hydrogel matrix should satisfy for biomedical and biotechnological applications are the matrix viscoelasticity and the ability of stress relaxation. Although alginate satisfies both of them, experimental data note that cell growth is significantly reduced by microenvironmental effects. Microenvironmental restriction effects are connected to matrix resistance stress accumulation. Matrix stress is generated within the boundary layers around the cell aggregates under compression caused by cell rearrangement and growth. Simultaneously induced relaxation phenomena of both subsystems: (1) immobilized cells and (2) hydrogel matrix occur at three time scales through successive relaxation cycles. Complex dynamics of matrix compression intensifies mechanical and electrostatic cell-matrix interactions. Minimizing of the resistance stress is the strategy for improving the matrix performances. Cell action could be simulated in the experiments... without cells during repeated stress relaxation cycles. We consider the physical and chemical modifications of Ca-alginate hydrogel.
Keywords:
Bioprocess optimization / Hydrogel matrix / Immobilized cells / Mathematical modeling / Matrix resistance stressSource:
Materials for Biomedical Engineering: Bioactive Materials, Properties, and Applications, 2019, 281-306Publisher:
- Elsevier
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Poljoprivredni fakultetTY - CHAP AU - Pajić-Lijaković, Ivana AU - Milivojević, M. AU - Lević, Steva AU - Trifković, Kata AU - Balanc, Bojana AU - Nedović, Viktor AU - Dajić-Stevanović, Zora AU - Radošević, Radenko AU - Bugarski, Branko PY - 2019 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/5142 AB - Some of the main rheological properties that Ca-alginate hydrogel matrix should satisfy for biomedical and biotechnological applications are the matrix viscoelasticity and the ability of stress relaxation. Although alginate satisfies both of them, experimental data note that cell growth is significantly reduced by microenvironmental effects. Microenvironmental restriction effects are connected to matrix resistance stress accumulation. Matrix stress is generated within the boundary layers around the cell aggregates under compression caused by cell rearrangement and growth. Simultaneously induced relaxation phenomena of both subsystems: (1) immobilized cells and (2) hydrogel matrix occur at three time scales through successive relaxation cycles. Complex dynamics of matrix compression intensifies mechanical and electrostatic cell-matrix interactions. Minimizing of the resistance stress is the strategy for improving the matrix performances. Cell action could be simulated in the experiments without cells during repeated stress relaxation cycles. We consider the physical and chemical modifications of Ca-alginate hydrogel. PB - Elsevier T2 - Materials for Biomedical Engineering: Bioactive Materials, Properties, and Applications T1 - Matrix resistance stress reduction-prerequisite for achieving higher concentration of immobilized cells EP - 306 SP - 281 DO - 10.1016/B978-0-12-818431-8.00009-X ER -
@inbook{ author = "Pajić-Lijaković, Ivana and Milivojević, M. and Lević, Steva and Trifković, Kata and Balanc, Bojana and Nedović, Viktor and Dajić-Stevanović, Zora and Radošević, Radenko and Bugarski, Branko", year = "2019", abstract = "Some of the main rheological properties that Ca-alginate hydrogel matrix should satisfy for biomedical and biotechnological applications are the matrix viscoelasticity and the ability of stress relaxation. Although alginate satisfies both of them, experimental data note that cell growth is significantly reduced by microenvironmental effects. Microenvironmental restriction effects are connected to matrix resistance stress accumulation. Matrix stress is generated within the boundary layers around the cell aggregates under compression caused by cell rearrangement and growth. Simultaneously induced relaxation phenomena of both subsystems: (1) immobilized cells and (2) hydrogel matrix occur at three time scales through successive relaxation cycles. Complex dynamics of matrix compression intensifies mechanical and electrostatic cell-matrix interactions. Minimizing of the resistance stress is the strategy for improving the matrix performances. Cell action could be simulated in the experiments without cells during repeated stress relaxation cycles. We consider the physical and chemical modifications of Ca-alginate hydrogel.", publisher = "Elsevier", journal = "Materials for Biomedical Engineering: Bioactive Materials, Properties, and Applications", booktitle = "Matrix resistance stress reduction-prerequisite for achieving higher concentration of immobilized cells", pages = "306-281", doi = "10.1016/B978-0-12-818431-8.00009-X" }
Pajić-Lijaković, I., Milivojević, M., Lević, S., Trifković, K., Balanc, B., Nedović, V., Dajić-Stevanović, Z., Radošević, R.,& Bugarski, B.. (2019). Matrix resistance stress reduction-prerequisite for achieving higher concentration of immobilized cells. in Materials for Biomedical Engineering: Bioactive Materials, Properties, and Applications Elsevier., 281-306. https://doi.org/10.1016/B978-0-12-818431-8.00009-X
Pajić-Lijaković I, Milivojević M, Lević S, Trifković K, Balanc B, Nedović V, Dajić-Stevanović Z, Radošević R, Bugarski B. Matrix resistance stress reduction-prerequisite for achieving higher concentration of immobilized cells. in Materials for Biomedical Engineering: Bioactive Materials, Properties, and Applications. 2019;:281-306. doi:10.1016/B978-0-12-818431-8.00009-X .
Pajić-Lijaković, Ivana, Milivojević, M., Lević, Steva, Trifković, Kata, Balanc, Bojana, Nedović, Viktor, Dajić-Stevanović, Zora, Radošević, Radenko, Bugarski, Branko, "Matrix resistance stress reduction-prerequisite for achieving higher concentration of immobilized cells" in Materials for Biomedical Engineering: Bioactive Materials, Properties, and Applications (2019):281-306, https://doi.org/10.1016/B978-0-12-818431-8.00009-X . .