Cell immobilisation by electrostatic droplet generation
Nema prikaza
Autori
Nedović, ViktorObradović, B
Poncelet, Denis
Goosen, MFA
Leskošek-Čukalović, Ida
Bugarski, Branko
Konferencijski prilog (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
This paper reviews the feasibility of electrostatic droplet generation for the production of uniform hydrogel microbeads and applications of this technique for cell immobilization. This is a novel extrusion technique that uses electrostatic forces to disrupt a liquid surface at the capillary/needle tip and form a charged stream of small droplets. Experimental parameters which are critical for production of polymer microbeads (in the range of 0.1 to 1 mm in diameter), as well as mechanisms of alginate droplet formation are presented here. It was shown that microbead size was a function of applied potential, polymer surface tension, needle size and electrode geometry. In addition, this technique was applied for immobilization of several cell types (yeast, mammalian and plant cells). There was no detectable loss in viability of these cell cultures after exposure to high electrostatic potentials. Cultivation studies of cells immobilized by electrostatic droplet generation showed good maint...enance of cell viability and activity, indicating broad potential of this technique for the immobilization of a variety of cell types for applications in different fields of biotechnology, pharamceuticals and medicine.
Ključne reči:
electrostatic droplet generation / cell immobilization / alginate carrier / microbeadIzvor:
Practical Aspects of Encapsulation Technologies, 2002, 11-17Izdavač:
- Bundesforschungsanstalt Landwirtschaft (Fal), Braunschweig
Institucija/grupa
Poljoprivredni fakultetTY - CONF AU - Nedović, Viktor AU - Obradović, B AU - Poncelet, Denis AU - Goosen, MFA AU - Leskošek-Čukalović, Ida AU - Bugarski, Branko PY - 2002 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/506 AB - This paper reviews the feasibility of electrostatic droplet generation for the production of uniform hydrogel microbeads and applications of this technique for cell immobilization. This is a novel extrusion technique that uses electrostatic forces to disrupt a liquid surface at the capillary/needle tip and form a charged stream of small droplets. Experimental parameters which are critical for production of polymer microbeads (in the range of 0.1 to 1 mm in diameter), as well as mechanisms of alginate droplet formation are presented here. It was shown that microbead size was a function of applied potential, polymer surface tension, needle size and electrode geometry. In addition, this technique was applied for immobilization of several cell types (yeast, mammalian and plant cells). There was no detectable loss in viability of these cell cultures after exposure to high electrostatic potentials. Cultivation studies of cells immobilized by electrostatic droplet generation showed good maintenance of cell viability and activity, indicating broad potential of this technique for the immobilization of a variety of cell types for applications in different fields of biotechnology, pharamceuticals and medicine. PB - Bundesforschungsanstalt Landwirtschaft (Fal), Braunschweig C3 - Practical Aspects of Encapsulation Technologies T1 - Cell immobilisation by electrostatic droplet generation EP - 17 SP - 11 UR - https://hdl.handle.net/21.15107/rcub_agrospace_506 ER -
@conference{ author = "Nedović, Viktor and Obradović, B and Poncelet, Denis and Goosen, MFA and Leskošek-Čukalović, Ida and Bugarski, Branko", year = "2002", abstract = "This paper reviews the feasibility of electrostatic droplet generation for the production of uniform hydrogel microbeads and applications of this technique for cell immobilization. This is a novel extrusion technique that uses electrostatic forces to disrupt a liquid surface at the capillary/needle tip and form a charged stream of small droplets. Experimental parameters which are critical for production of polymer microbeads (in the range of 0.1 to 1 mm in diameter), as well as mechanisms of alginate droplet formation are presented here. It was shown that microbead size was a function of applied potential, polymer surface tension, needle size and electrode geometry. In addition, this technique was applied for immobilization of several cell types (yeast, mammalian and plant cells). There was no detectable loss in viability of these cell cultures after exposure to high electrostatic potentials. Cultivation studies of cells immobilized by electrostatic droplet generation showed good maintenance of cell viability and activity, indicating broad potential of this technique for the immobilization of a variety of cell types for applications in different fields of biotechnology, pharamceuticals and medicine.", publisher = "Bundesforschungsanstalt Landwirtschaft (Fal), Braunschweig", journal = "Practical Aspects of Encapsulation Technologies", title = "Cell immobilisation by electrostatic droplet generation", pages = "17-11", url = "https://hdl.handle.net/21.15107/rcub_agrospace_506" }
Nedović, V., Obradović, B., Poncelet, D., Goosen, M., Leskošek-Čukalović, I.,& Bugarski, B.. (2002). Cell immobilisation by electrostatic droplet generation. in Practical Aspects of Encapsulation Technologies Bundesforschungsanstalt Landwirtschaft (Fal), Braunschweig., 11-17. https://hdl.handle.net/21.15107/rcub_agrospace_506
Nedović V, Obradović B, Poncelet D, Goosen M, Leskošek-Čukalović I, Bugarski B. Cell immobilisation by electrostatic droplet generation. in Practical Aspects of Encapsulation Technologies. 2002;:11-17. https://hdl.handle.net/21.15107/rcub_agrospace_506 .
Nedović, Viktor, Obradović, B, Poncelet, Denis, Goosen, MFA, Leskošek-Čukalović, Ida, Bugarski, Branko, "Cell immobilisation by electrostatic droplet generation" in Practical Aspects of Encapsulation Technologies (2002):11-17, https://hdl.handle.net/21.15107/rcub_agrospace_506 .