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Cell immobilisation by electrostatic droplet generation

Нема приказа
Аутори
Nedović, Viktor
Obradović, B
Poncelet, Denis
Goosen, MFA
Leskošek-Čukalović, Ida
Bugarski, Branko
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документу
Апстракт
This paper reviews the feasibility of electrostatic droplet generation for the production of uniform hydrogel microbeads and applications of this technique for cell immobilization. This is a novel extrusion technique that uses electrostatic forces to disrupt a liquid surface at the capillary/needle tip and form a charged stream of small droplets. Experimental parameters which are critical for production of polymer microbeads (in the range of 0.1 to 1 mm in diameter), as well as mechanisms of alginate droplet formation are presented here. It was shown that microbead size was a function of applied potential, polymer surface tension, needle size and electrode geometry. In addition, this technique was applied for immobilization of several cell types (yeast, mammalian and plant cells). There was no detectable loss in viability of these cell cultures after exposure to high electrostatic potentials. Cultivation studies of cells immobilized by electrostatic droplet generation showed good maint...enance of cell viability and activity, indicating broad potential of this technique for the immobilization of a variety of cell types for applications in different fields of biotechnology, pharamceuticals and medicine.

Кључне речи:
electrostatic droplet generation / cell immobilization / alginate carrier / microbead
Извор:
Practical Aspects of Encapsulation Technologies, 2002, 11-17
Издавач:
  • Bundesforschungsanstalt Landwirtschaft (Fal), Braunschweig

WoS: 000182532400002

[ Google Scholar ]
12
Handle
https://hdl.handle.net/21.15107/rcub_agrospace_506
URI
http://aspace.agrif.bg.ac.rs/handle/123456789/506
Колекције
  • Radovi istraživača / Researchers’ publications
Институција/група
Poljoprivredni fakultet
TY  - CONF
AU  - Nedović, Viktor
AU  - Obradović, B
AU  - Poncelet, Denis
AU  - Goosen, MFA
AU  - Leskošek-Čukalović, Ida
AU  - Bugarski, Branko
PY  - 2002
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/506
AB  - This paper reviews the feasibility of electrostatic droplet generation for the production of uniform hydrogel microbeads and applications of this technique for cell immobilization. This is a novel extrusion technique that uses electrostatic forces to disrupt a liquid surface at the capillary/needle tip and form a charged stream of small droplets. Experimental parameters which are critical for production of polymer microbeads (in the range of 0.1 to 1 mm in diameter), as well as mechanisms of alginate droplet formation are presented here. It was shown that microbead size was a function of applied potential, polymer surface tension, needle size and electrode geometry. In addition, this technique was applied for immobilization of several cell types (yeast, mammalian and plant cells). There was no detectable loss in viability of these cell cultures after exposure to high electrostatic potentials. Cultivation studies of cells immobilized by electrostatic droplet generation showed good maintenance of cell viability and activity, indicating broad potential of this technique for the immobilization of a variety of cell types for applications in different fields of biotechnology, pharamceuticals and medicine.
PB  - Bundesforschungsanstalt Landwirtschaft (Fal), Braunschweig
C3  - Practical Aspects of Encapsulation Technologies
T1  - Cell immobilisation by electrostatic droplet generation
EP  - 17
SP  - 11
UR  - conv_4120
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_506
ER  - 
@conference{
author = "Nedović, Viktor and Obradović, B and Poncelet, Denis and Goosen, MFA and Leskošek-Čukalović, Ida and Bugarski, Branko",
year = "2002",
abstract = "This paper reviews the feasibility of electrostatic droplet generation for the production of uniform hydrogel microbeads and applications of this technique for cell immobilization. This is a novel extrusion technique that uses electrostatic forces to disrupt a liquid surface at the capillary/needle tip and form a charged stream of small droplets. Experimental parameters which are critical for production of polymer microbeads (in the range of 0.1 to 1 mm in diameter), as well as mechanisms of alginate droplet formation are presented here. It was shown that microbead size was a function of applied potential, polymer surface tension, needle size and electrode geometry. In addition, this technique was applied for immobilization of several cell types (yeast, mammalian and plant cells). There was no detectable loss in viability of these cell cultures after exposure to high electrostatic potentials. Cultivation studies of cells immobilized by electrostatic droplet generation showed good maintenance of cell viability and activity, indicating broad potential of this technique for the immobilization of a variety of cell types for applications in different fields of biotechnology, pharamceuticals and medicine.",
publisher = "Bundesforschungsanstalt Landwirtschaft (Fal), Braunschweig",
journal = "Practical Aspects of Encapsulation Technologies",
title = "Cell immobilisation by electrostatic droplet generation",
pages = "17-11",
url = "conv_4120, https://hdl.handle.net/21.15107/rcub_agrospace_506"
}
Nedović, V., Obradović, B., Poncelet, D., Goosen, M., Leskošek-Čukalović, I.,& Bugarski, B.. (2002). Cell immobilisation by electrostatic droplet generation. in Practical Aspects of Encapsulation Technologies
Bundesforschungsanstalt Landwirtschaft (Fal), Braunschweig., 11-17.
conv_4120
Nedović V, Obradović B, Poncelet D, Goosen M, Leskošek-Čukalović I, Bugarski B. Cell immobilisation by electrostatic droplet generation. in Practical Aspects of Encapsulation Technologies. 2002;:11-17.
conv_4120 .
Nedović, Viktor, Obradović, B, Poncelet, Denis, Goosen, MFA, Leskošek-Čukalović, Ida, Bugarski, Branko, "Cell immobilisation by electrostatic droplet generation" in Practical Aspects of Encapsulation Technologies (2002):11-17,
conv_4120 .

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