Effect of gentisic acid on the structural-functional properties of liposomes incorporating beta-sitosterol
Само за регистроване кориснике
2019
Аутори
Jovanović, Aleksandra A.Balanc, Bojana
Djordjević, Verica B.
Ota, Ajda
Skrt, Mihaela
Savikin, Katarina
Bugarski, Branko
Nedović, Viktor
Ulrih, Nataša Poklar
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Multifunctional liposomes incorporating beta-sitosterol were developed for delivery of gentisic acid (GA). The interactions of both compounds with phospholipid bilayer were interpreted viaeffects of different beta-sitosterol content (0, 20 and 50 mol %) and different gentisic acid to lipid ratio (n(GA)/n(lip) from 10(-5) to 1) on membrane fluidity and thermotropic properties. Multilamellar vesicles of phosphatidylcholines (with size range between 1350 and 1900 nm) effectively encapsulated GA (54%) when n(GA)/n(lip) was higher than 0.01. Suppression of lipid peroxidation was directly related to concentration of GA. The resistance to diffusion of gentisic acid from liposomes increased for (similar to)50% in samples incorporating 50 mol % beta-sitosterol compared to sterol-free liposomes. Finally, simulated in vitro gastrointestinal conditions showed that the release was mainly affected by low pH of simulated gastric fluid and the presence of cholates in simulated intestinal fluid, rather... than by enzymes activity.
Кључне речи:
Gentisic acid / Liposomes / beta-sitosterol / Release study / Membrane fluidity / PhosphatidylcholinesИзвор:
Colloids and Surfaces B-Biointerfaces, 2019, 183Издавач:
- Elsevier, Amsterdam
Финансирање / пројекти:
- Slovenian Research AgencySlovenian Research Agency - Slovenia [P4-0121]
- bilateral project Serbia
- bilateral project Slovenia
- Развој нових инкапсулационих и ензимских технологија за производњу биокатализатора и биолошки активних компонената хране у циљу повећања њене конкурентности, квалитета и безбедности (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
- Традиционални и нови производи од плодова гајених и самониклих врста воћака и винове лозе и нус-продуката у преради, са посебним освртом на аутохтоне сорте: хемијска карактеризација и биолошки профил (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46013)
DOI: 10.1016/j.colsurfb.2019.110422
ISSN: 0927-7765
PubMed: 31437609
WoS: 000494048600029
Scopus: 2-s2.0-85070688519
Институција/група
Poljoprivredni fakultetTY - JOUR AU - Jovanović, Aleksandra A. AU - Balanc, Bojana AU - Djordjević, Verica B. AU - Ota, Ajda AU - Skrt, Mihaela AU - Savikin, Katarina AU - Bugarski, Branko AU - Nedović, Viktor AU - Ulrih, Nataša Poklar PY - 2019 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/4992 AB - Multifunctional liposomes incorporating beta-sitosterol were developed for delivery of gentisic acid (GA). The interactions of both compounds with phospholipid bilayer were interpreted viaeffects of different beta-sitosterol content (0, 20 and 50 mol %) and different gentisic acid to lipid ratio (n(GA)/n(lip) from 10(-5) to 1) on membrane fluidity and thermotropic properties. Multilamellar vesicles of phosphatidylcholines (with size range between 1350 and 1900 nm) effectively encapsulated GA (54%) when n(GA)/n(lip) was higher than 0.01. Suppression of lipid peroxidation was directly related to concentration of GA. The resistance to diffusion of gentisic acid from liposomes increased for (similar to)50% in samples incorporating 50 mol % beta-sitosterol compared to sterol-free liposomes. Finally, simulated in vitro gastrointestinal conditions showed that the release was mainly affected by low pH of simulated gastric fluid and the presence of cholates in simulated intestinal fluid, rather than by enzymes activity. PB - Elsevier, Amsterdam T2 - Colloids and Surfaces B-Biointerfaces T1 - Effect of gentisic acid on the structural-functional properties of liposomes incorporating beta-sitosterol VL - 183 DO - 10.1016/j.colsurfb.2019.110422 ER -
@article{ author = "Jovanović, Aleksandra A. and Balanc, Bojana and Djordjević, Verica B. and Ota, Ajda and Skrt, Mihaela and Savikin, Katarina and Bugarski, Branko and Nedović, Viktor and Ulrih, Nataša Poklar", year = "2019", abstract = "Multifunctional liposomes incorporating beta-sitosterol were developed for delivery of gentisic acid (GA). The interactions of both compounds with phospholipid bilayer were interpreted viaeffects of different beta-sitosterol content (0, 20 and 50 mol %) and different gentisic acid to lipid ratio (n(GA)/n(lip) from 10(-5) to 1) on membrane fluidity and thermotropic properties. Multilamellar vesicles of phosphatidylcholines (with size range between 1350 and 1900 nm) effectively encapsulated GA (54%) when n(GA)/n(lip) was higher than 0.01. Suppression of lipid peroxidation was directly related to concentration of GA. The resistance to diffusion of gentisic acid from liposomes increased for (similar to)50% in samples incorporating 50 mol % beta-sitosterol compared to sterol-free liposomes. Finally, simulated in vitro gastrointestinal conditions showed that the release was mainly affected by low pH of simulated gastric fluid and the presence of cholates in simulated intestinal fluid, rather than by enzymes activity.", publisher = "Elsevier, Amsterdam", journal = "Colloids and Surfaces B-Biointerfaces", title = "Effect of gentisic acid on the structural-functional properties of liposomes incorporating beta-sitosterol", volume = "183", doi = "10.1016/j.colsurfb.2019.110422" }
Jovanović, A. A., Balanc, B., Djordjević, V. B., Ota, A., Skrt, M., Savikin, K., Bugarski, B., Nedović, V.,& Ulrih, N. P.. (2019). Effect of gentisic acid on the structural-functional properties of liposomes incorporating beta-sitosterol. in Colloids and Surfaces B-Biointerfaces Elsevier, Amsterdam., 183. https://doi.org/10.1016/j.colsurfb.2019.110422
Jovanović AA, Balanc B, Djordjević VB, Ota A, Skrt M, Savikin K, Bugarski B, Nedović V, Ulrih NP. Effect of gentisic acid on the structural-functional properties of liposomes incorporating beta-sitosterol. in Colloids and Surfaces B-Biointerfaces. 2019;183. doi:10.1016/j.colsurfb.2019.110422 .
Jovanović, Aleksandra A., Balanc, Bojana, Djordjević, Verica B., Ota, Ajda, Skrt, Mihaela, Savikin, Katarina, Bugarski, Branko, Nedović, Viktor, Ulrih, Nataša Poklar, "Effect of gentisic acid on the structural-functional properties of liposomes incorporating beta-sitosterol" in Colloids and Surfaces B-Biointerfaces, 183 (2019), https://doi.org/10.1016/j.colsurfb.2019.110422 . .