Hepatoprotective effect of fullerenol/doxorubicin nanocomposite in acute treatment of healthy rats
Samo za registrovane korisnike
2018
Autori
Petrović, DanijelaSeke, Mariana
Labudović-Borović, Milica
Jović, Danica
Borisev, Ivana
Srdjenović, Branislava
Rakocević, Zlatko
Pavlović, Vladimir
Djordjević, Aleksandar
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
In our recent studies we have designed fullerenol/doxorubicin nanocomposite (FNP/DOX) as the new drug nanocarrier. This research has demonstrated that this novel nanocomposite has had better implications on the liver tissue in vivo (Wistar rats treated intraperitoneally), than treatment based only on DOX. FNP/DOX has been characterised by DLS, TEM and AFM measurements which have shown that DOX loaded onto FNP did not influence fullerenol nanoparticle's size. FNP/DOX affected oxidative status in blood causing a significant decrease of catalase and SOD activity in comparison to DOX, implicating the reduction in oxidative stress. qRT-PCR results on the mRNA level of antioxidative enzymes (catalase and MnSOD) revealed that the effect of oxidative stress is significantly reduced by the treatment with FNP/DOX (p lt .05). The ultrastructural analysis of the liver tissue has revealed that FNP/DOX nanocomposite generated considerably less damage in the liver tissue, than DOX applied at the same... dose. Hence, our results have indicated that FNP, within FNP/DOX nanocomposite, exhibits protective effects to the liver tissue of the healthy rats.
Ključne reči:
Fullerenol/doxorubicin nanocomposite / Drug delivery / AFM / qRT-PCR / Ultrastructural analysisIzvor:
Experimental and Molecular Pathology, 2018, 104, 3, 199-211Izdavač:
- Academic Press Inc Elsevier Science, San Diego
Finansiranje / projekti:
DOI: 10.1016/j.yexmp.2018.04.005
ISSN: 0014-4800
PubMed: 29727604
WoS: 000434907600005
Scopus: 2-s2.0-85046811167
Institucija/grupa
Poljoprivredni fakultetTY - JOUR AU - Petrović, Danijela AU - Seke, Mariana AU - Labudović-Borović, Milica AU - Jović, Danica AU - Borisev, Ivana AU - Srdjenović, Branislava AU - Rakocević, Zlatko AU - Pavlović, Vladimir AU - Djordjević, Aleksandar PY - 2018 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/4794 AB - In our recent studies we have designed fullerenol/doxorubicin nanocomposite (FNP/DOX) as the new drug nanocarrier. This research has demonstrated that this novel nanocomposite has had better implications on the liver tissue in vivo (Wistar rats treated intraperitoneally), than treatment based only on DOX. FNP/DOX has been characterised by DLS, TEM and AFM measurements which have shown that DOX loaded onto FNP did not influence fullerenol nanoparticle's size. FNP/DOX affected oxidative status in blood causing a significant decrease of catalase and SOD activity in comparison to DOX, implicating the reduction in oxidative stress. qRT-PCR results on the mRNA level of antioxidative enzymes (catalase and MnSOD) revealed that the effect of oxidative stress is significantly reduced by the treatment with FNP/DOX (p lt .05). The ultrastructural analysis of the liver tissue has revealed that FNP/DOX nanocomposite generated considerably less damage in the liver tissue, than DOX applied at the same dose. Hence, our results have indicated that FNP, within FNP/DOX nanocomposite, exhibits protective effects to the liver tissue of the healthy rats. PB - Academic Press Inc Elsevier Science, San Diego T2 - Experimental and Molecular Pathology T1 - Hepatoprotective effect of fullerenol/doxorubicin nanocomposite in acute treatment of healthy rats EP - 211 IS - 3 SP - 199 VL - 104 DO - 10.1016/j.yexmp.2018.04.005 ER -
@article{ author = "Petrović, Danijela and Seke, Mariana and Labudović-Borović, Milica and Jović, Danica and Borisev, Ivana and Srdjenović, Branislava and Rakocević, Zlatko and Pavlović, Vladimir and Djordjević, Aleksandar", year = "2018", abstract = "In our recent studies we have designed fullerenol/doxorubicin nanocomposite (FNP/DOX) as the new drug nanocarrier. This research has demonstrated that this novel nanocomposite has had better implications on the liver tissue in vivo (Wistar rats treated intraperitoneally), than treatment based only on DOX. FNP/DOX has been characterised by DLS, TEM and AFM measurements which have shown that DOX loaded onto FNP did not influence fullerenol nanoparticle's size. FNP/DOX affected oxidative status in blood causing a significant decrease of catalase and SOD activity in comparison to DOX, implicating the reduction in oxidative stress. qRT-PCR results on the mRNA level of antioxidative enzymes (catalase and MnSOD) revealed that the effect of oxidative stress is significantly reduced by the treatment with FNP/DOX (p lt .05). The ultrastructural analysis of the liver tissue has revealed that FNP/DOX nanocomposite generated considerably less damage in the liver tissue, than DOX applied at the same dose. Hence, our results have indicated that FNP, within FNP/DOX nanocomposite, exhibits protective effects to the liver tissue of the healthy rats.", publisher = "Academic Press Inc Elsevier Science, San Diego", journal = "Experimental and Molecular Pathology", title = "Hepatoprotective effect of fullerenol/doxorubicin nanocomposite in acute treatment of healthy rats", pages = "211-199", number = "3", volume = "104", doi = "10.1016/j.yexmp.2018.04.005" }
Petrović, D., Seke, M., Labudović-Borović, M., Jović, D., Borisev, I., Srdjenović, B., Rakocević, Z., Pavlović, V.,& Djordjević, A.. (2018). Hepatoprotective effect of fullerenol/doxorubicin nanocomposite in acute treatment of healthy rats. in Experimental and Molecular Pathology Academic Press Inc Elsevier Science, San Diego., 104(3), 199-211. https://doi.org/10.1016/j.yexmp.2018.04.005
Petrović D, Seke M, Labudović-Borović M, Jović D, Borisev I, Srdjenović B, Rakocević Z, Pavlović V, Djordjević A. Hepatoprotective effect of fullerenol/doxorubicin nanocomposite in acute treatment of healthy rats. in Experimental and Molecular Pathology. 2018;104(3):199-211. doi:10.1016/j.yexmp.2018.04.005 .
Petrović, Danijela, Seke, Mariana, Labudović-Borović, Milica, Jović, Danica, Borisev, Ivana, Srdjenović, Branislava, Rakocević, Zlatko, Pavlović, Vladimir, Djordjević, Aleksandar, "Hepatoprotective effect of fullerenol/doxorubicin nanocomposite in acute treatment of healthy rats" in Experimental and Molecular Pathology, 104, no. 3 (2018):199-211, https://doi.org/10.1016/j.yexmp.2018.04.005 . .