Bactericidal activity of Cu-, Zn-, and Ag-containing zeolites toward Escherichia coli isolates
Abstract
Two types of zeolites-natural clinoptilolite (NZ) and synthetic zeolite A (A)-were enriched with approx. 0.25 mmol of Cu(II), Zn(II), or Ag(I) ions, and the obtained materials (M-Z) were tested against three different isolates of Escherichia coli. Two isolates were environmental isolates from waters in Serbia whereas the third one was DSM 498. Antibacterial activity was studied in different water media-nutrient-rich media (peptone water), water from Sava Lake, and commercially available spring water. The Ag-containing zeolites showed bactericidal activity in the nutrient-rich peptone water after 1 h of contact. Cu- and Zn-containing zeolites showed bactericidal activity in real water samples. Antibacterial activity of the M-Z decreases in all three examined water media in the following order: Ag-NZ ae Ag-A > Cu-NZ ae Cu-A > Zn-NZ >>> Zn-A, suggesting that mainly the metal type and not the zeolite type have a role in the antibacterial activity. Leaching experiments showed small amounts ...of the leached Cu(II) and Zn(II) ions, indicating that the antibacterial activity is not due to the metal ions but should be attributed to the M-Z itself. However, leached amounts of Ag(I) from Ag-NZ and Ag-A in peptone water indicate that the released Ag(I) could be mainly responsible for the bactericidal effect of the Ag(I)-containing zeolites. Since no loss of cellular material was found, the antibacterial activity is not attributed to cytoplasmic membrane damage.
Keywords:
Clinoptilolite / Zeolite A / Copper / Zinc / Silver / Escherichia coli / Alternative disinfectantsSource:
Environmental Science and Pollution Research, 2017, 24, 25, 20273-20281Publisher:
- Springer Heidelberg, Heidelberg
Funding / projects:
- Croatian Science Foundation [IP-2014-09-5656]
- Oxide-based environmentally-friendly porous materials for genotoxic substances removal (RS-MESTD-Basic Research (BR or ON)-172018)
- Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
DOI: 10.1007/s11356-017-9643-8
ISSN: 0944-1344
PubMed: 28702914
WoS: 000408698700012
Scopus: 2-s2.0-85023161386
Collections
Institution/Community
Poljoprivredni fakultetTY - JOUR AU - Milenković, Jelena AU - Hrenović, Jasna AU - Matijasević, Danka AU - Nikšić, Miomir AU - Rajić, Nevenka PY - 2017 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/4455 AB - Two types of zeolites-natural clinoptilolite (NZ) and synthetic zeolite A (A)-were enriched with approx. 0.25 mmol of Cu(II), Zn(II), or Ag(I) ions, and the obtained materials (M-Z) were tested against three different isolates of Escherichia coli. Two isolates were environmental isolates from waters in Serbia whereas the third one was DSM 498. Antibacterial activity was studied in different water media-nutrient-rich media (peptone water), water from Sava Lake, and commercially available spring water. The Ag-containing zeolites showed bactericidal activity in the nutrient-rich peptone water after 1 h of contact. Cu- and Zn-containing zeolites showed bactericidal activity in real water samples. Antibacterial activity of the M-Z decreases in all three examined water media in the following order: Ag-NZ ae Ag-A > Cu-NZ ae Cu-A > Zn-NZ >>> Zn-A, suggesting that mainly the metal type and not the zeolite type have a role in the antibacterial activity. Leaching experiments showed small amounts of the leached Cu(II) and Zn(II) ions, indicating that the antibacterial activity is not due to the metal ions but should be attributed to the M-Z itself. However, leached amounts of Ag(I) from Ag-NZ and Ag-A in peptone water indicate that the released Ag(I) could be mainly responsible for the bactericidal effect of the Ag(I)-containing zeolites. Since no loss of cellular material was found, the antibacterial activity is not attributed to cytoplasmic membrane damage. PB - Springer Heidelberg, Heidelberg T2 - Environmental Science and Pollution Research T1 - Bactericidal activity of Cu-, Zn-, and Ag-containing zeolites toward Escherichia coli isolates EP - 20281 IS - 25 SP - 20273 VL - 24 DO - 10.1007/s11356-017-9643-8 ER -
@article{ author = "Milenković, Jelena and Hrenović, Jasna and Matijasević, Danka and Nikšić, Miomir and Rajić, Nevenka", year = "2017", abstract = "Two types of zeolites-natural clinoptilolite (NZ) and synthetic zeolite A (A)-were enriched with approx. 0.25 mmol of Cu(II), Zn(II), or Ag(I) ions, and the obtained materials (M-Z) were tested against three different isolates of Escherichia coli. Two isolates were environmental isolates from waters in Serbia whereas the third one was DSM 498. Antibacterial activity was studied in different water media-nutrient-rich media (peptone water), water from Sava Lake, and commercially available spring water. The Ag-containing zeolites showed bactericidal activity in the nutrient-rich peptone water after 1 h of contact. Cu- and Zn-containing zeolites showed bactericidal activity in real water samples. Antibacterial activity of the M-Z decreases in all three examined water media in the following order: Ag-NZ ae Ag-A > Cu-NZ ae Cu-A > Zn-NZ >>> Zn-A, suggesting that mainly the metal type and not the zeolite type have a role in the antibacterial activity. Leaching experiments showed small amounts of the leached Cu(II) and Zn(II) ions, indicating that the antibacterial activity is not due to the metal ions but should be attributed to the M-Z itself. However, leached amounts of Ag(I) from Ag-NZ and Ag-A in peptone water indicate that the released Ag(I) could be mainly responsible for the bactericidal effect of the Ag(I)-containing zeolites. Since no loss of cellular material was found, the antibacterial activity is not attributed to cytoplasmic membrane damage.", publisher = "Springer Heidelberg, Heidelberg", journal = "Environmental Science and Pollution Research", title = "Bactericidal activity of Cu-, Zn-, and Ag-containing zeolites toward Escherichia coli isolates", pages = "20281-20273", number = "25", volume = "24", doi = "10.1007/s11356-017-9643-8" }
Milenković, J., Hrenović, J., Matijasević, D., Nikšić, M.,& Rajić, N.. (2017). Bactericidal activity of Cu-, Zn-, and Ag-containing zeolites toward Escherichia coli isolates. in Environmental Science and Pollution Research Springer Heidelberg, Heidelberg., 24(25), 20273-20281. https://doi.org/10.1007/s11356-017-9643-8
Milenković J, Hrenović J, Matijasević D, Nikšić M, Rajić N. Bactericidal activity of Cu-, Zn-, and Ag-containing zeolites toward Escherichia coli isolates. in Environmental Science and Pollution Research. 2017;24(25):20273-20281. doi:10.1007/s11356-017-9643-8 .
Milenković, Jelena, Hrenović, Jasna, Matijasević, Danka, Nikšić, Miomir, Rajić, Nevenka, "Bactericidal activity of Cu-, Zn-, and Ag-containing zeolites toward Escherichia coli isolates" in Environmental Science and Pollution Research, 24, no. 25 (2017):20273-20281, https://doi.org/10.1007/s11356-017-9643-8 . .