The Antibacterial Activity of Coriolus versicolor Methanol Extract and Its Effect on Ultrastructural Changes of Staphylococcus aureus and Salmonella Enteritidis
2016
Authors
Matijasević, DankaPantić, Milena
Rašković, Božidar
Pavlović, Vladimir
Miletić, Dunja
Sknepnek, Aleksandra
Nikšić, Miomir
Article (Published version)
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The antibacterial activity of methanol extract obtained from fruiting body of industrially grown basidiomycete Coriolus versicolor was examined. The Minimum Inhibitory Concentration (MIC) values against various bacteria ranged from 0.625 to 20 mg mL(-1). versicolor expressed bactericidal activity against both Gram-positive and Gram-negative bacteria. The growth curves of Staphylococcus aureus and Salmonella enterica serovar Enteritidis, measured at 630 nm, and confirmed with macrodilution method showed that the obtained extract could inhibit the growth of tested bacteria. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), and the loss of 260-nm-absorbing material were used to examine the ultrastructural changes in bacteria induced by the extract. When S. aureus was exposed to the MIC of C. versicolor, elongated and malformed cells were observed by SEM, while S. Enteritidis treated cells appeared shorter and aggregated with ruptured cell walls. TEM revealed the ...formation of non-membrane-enclosed bodies and depleted inner content of S. aureus. Larger and irregular periplasmic space and deformed and scattered components of the cell envelope were observed in treated S. Enteritidis. The loss of 260-nm-absorbing material indicated that the disruptive action of the extract on cytoplasmic membrane was more pronounced in S. aureus than in S. Enteritidis treated cells. The UV and FTIR spectrophotometric analyses revealed diverse composition of C. versicolor extract and high content of total phenolics. Altogether, mushroom extracts could be used to develop nutraceuticals or drugs effective against pathogenic microorganisms.
Keywords:
antibacterial activity / natural preservatives / foodborne pathogens / mushroom extract / Coriolus versicolor / electron microscopySource:
Frontiers in Microbiology, 2016, 7Publisher:
- Frontiers Media Sa, Lausanne
Funding / projects:
- Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
- Directed synthesis, structure and properties of multifunctional materials (RS-MESTD-Basic Research (BR or ON)-172057)
DOI: 10.3389/fmicb.2016.01226
ISSN: 1664-302X
PubMed: 27540376
WoS: 000380662900001
Scopus: 2-s2.0-84988882813
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Poljoprivredni fakultetTY - JOUR AU - Matijasević, Danka AU - Pantić, Milena AU - Rašković, Božidar AU - Pavlović, Vladimir AU - Miletić, Dunja AU - Sknepnek, Aleksandra AU - Nikšić, Miomir PY - 2016 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/4017 AB - The antibacterial activity of methanol extract obtained from fruiting body of industrially grown basidiomycete Coriolus versicolor was examined. The Minimum Inhibitory Concentration (MIC) values against various bacteria ranged from 0.625 to 20 mg mL(-1). versicolor expressed bactericidal activity against both Gram-positive and Gram-negative bacteria. The growth curves of Staphylococcus aureus and Salmonella enterica serovar Enteritidis, measured at 630 nm, and confirmed with macrodilution method showed that the obtained extract could inhibit the growth of tested bacteria. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), and the loss of 260-nm-absorbing material were used to examine the ultrastructural changes in bacteria induced by the extract. When S. aureus was exposed to the MIC of C. versicolor, elongated and malformed cells were observed by SEM, while S. Enteritidis treated cells appeared shorter and aggregated with ruptured cell walls. TEM revealed the formation of non-membrane-enclosed bodies and depleted inner content of S. aureus. Larger and irregular periplasmic space and deformed and scattered components of the cell envelope were observed in treated S. Enteritidis. The loss of 260-nm-absorbing material indicated that the disruptive action of the extract on cytoplasmic membrane was more pronounced in S. aureus than in S. Enteritidis treated cells. The UV and FTIR spectrophotometric analyses revealed diverse composition of C. versicolor extract and high content of total phenolics. Altogether, mushroom extracts could be used to develop nutraceuticals or drugs effective against pathogenic microorganisms. PB - Frontiers Media Sa, Lausanne T2 - Frontiers in Microbiology T1 - The Antibacterial Activity of Coriolus versicolor Methanol Extract and Its Effect on Ultrastructural Changes of Staphylococcus aureus and Salmonella Enteritidis VL - 7 DO - 10.3389/fmicb.2016.01226 ER -
@article{ author = "Matijasević, Danka and Pantić, Milena and Rašković, Božidar and Pavlović, Vladimir and Miletić, Dunja and Sknepnek, Aleksandra and Nikšić, Miomir", year = "2016", abstract = "The antibacterial activity of methanol extract obtained from fruiting body of industrially grown basidiomycete Coriolus versicolor was examined. The Minimum Inhibitory Concentration (MIC) values against various bacteria ranged from 0.625 to 20 mg mL(-1). versicolor expressed bactericidal activity against both Gram-positive and Gram-negative bacteria. The growth curves of Staphylococcus aureus and Salmonella enterica serovar Enteritidis, measured at 630 nm, and confirmed with macrodilution method showed that the obtained extract could inhibit the growth of tested bacteria. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), and the loss of 260-nm-absorbing material were used to examine the ultrastructural changes in bacteria induced by the extract. When S. aureus was exposed to the MIC of C. versicolor, elongated and malformed cells were observed by SEM, while S. Enteritidis treated cells appeared shorter and aggregated with ruptured cell walls. TEM revealed the formation of non-membrane-enclosed bodies and depleted inner content of S. aureus. Larger and irregular periplasmic space and deformed and scattered components of the cell envelope were observed in treated S. Enteritidis. The loss of 260-nm-absorbing material indicated that the disruptive action of the extract on cytoplasmic membrane was more pronounced in S. aureus than in S. Enteritidis treated cells. The UV and FTIR spectrophotometric analyses revealed diverse composition of C. versicolor extract and high content of total phenolics. Altogether, mushroom extracts could be used to develop nutraceuticals or drugs effective against pathogenic microorganisms.", publisher = "Frontiers Media Sa, Lausanne", journal = "Frontiers in Microbiology", title = "The Antibacterial Activity of Coriolus versicolor Methanol Extract and Its Effect on Ultrastructural Changes of Staphylococcus aureus and Salmonella Enteritidis", volume = "7", doi = "10.3389/fmicb.2016.01226" }
Matijasević, D., Pantić, M., Rašković, B., Pavlović, V., Miletić, D., Sknepnek, A.,& Nikšić, M.. (2016). The Antibacterial Activity of Coriolus versicolor Methanol Extract and Its Effect on Ultrastructural Changes of Staphylococcus aureus and Salmonella Enteritidis. in Frontiers in Microbiology Frontiers Media Sa, Lausanne., 7. https://doi.org/10.3389/fmicb.2016.01226
Matijasević D, Pantić M, Rašković B, Pavlović V, Miletić D, Sknepnek A, Nikšić M. The Antibacterial Activity of Coriolus versicolor Methanol Extract and Its Effect on Ultrastructural Changes of Staphylococcus aureus and Salmonella Enteritidis. in Frontiers in Microbiology. 2016;7. doi:10.3389/fmicb.2016.01226 .
Matijasević, Danka, Pantić, Milena, Rašković, Božidar, Pavlović, Vladimir, Miletić, Dunja, Sknepnek, Aleksandra, Nikšić, Miomir, "The Antibacterial Activity of Coriolus versicolor Methanol Extract and Its Effect on Ultrastructural Changes of Staphylococcus aureus and Salmonella Enteritidis" in Frontiers in Microbiology, 7 (2016), https://doi.org/10.3389/fmicb.2016.01226 . .