Different toxicity of the strobilurin fungicides kresoxim-methyl and trifloxistrobin to Venturia inaequalis isolates from Serbia
Апстракт
Sensitivity of monosporial isolates of V. inaequalis, originated from apple orchards previously exposed (commercial and experimental) and those originated from location where fungicides had never been used (wild type), to strobilurin fungicides kresoxim-methyl and trifloxistrobin was investigated in this study. The experiments were carried out in laboratory using in vitro and in vivo methods. Mycelia growth inhibition on PDA medium with kresoxim-methyl and trifloxistrobin was monitored and sensitivity parameters (EC50 values) were determined using probit analysis. The isolates sensitivity in vivo was tested on apple seedlings using inoculation method. The EC50 values were ranging from 0.066-2.033 and 0.011-0.323 mu g mL(-1) for kresoxim-methyl and trifloxistrobin, respectively. The wild type of isolates showed a normal susceptibility to kresoxim-methyl and trifloxistrobin, while isolates originated from experimental and commercial orchard where those fungicides had been used extensivel...y, showed significantly lower sensitivity to the both strobilurin fungicides. The isolate that showed the resistance to kresoxim-methyl and trifloxistrobin in vitro test, also showed reduced sensitivity in vivo test and could not been controlled satisfactory by fungicide concentrations that are commercially used, as well as by double higher, after apple seedlings inoculation.
Кључне речи:
Venturia inaequalis / trifloxistrobin / kresoxim-methyl / sensitivity / resistanceИзвор:
Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Was, 2015, 50, 9, 633-637Издавач:
- Taylor & Francis Inc, Philadelphia
DOI: 10.1080/03601234.2015.1038952
ISSN: 0360-1234
PubMed: 26079336
WoS: 000356340700001
Scopus: 2-s2.0-84931059042
Институција/група
Poljoprivredni fakultetTY - JOUR AU - Stević, Milan AU - Tamaš, Nenad AU - Miletić, Novica AU - Vukša, Petar PY - 2015 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/3835 AB - Sensitivity of monosporial isolates of V. inaequalis, originated from apple orchards previously exposed (commercial and experimental) and those originated from location where fungicides had never been used (wild type), to strobilurin fungicides kresoxim-methyl and trifloxistrobin was investigated in this study. The experiments were carried out in laboratory using in vitro and in vivo methods. Mycelia growth inhibition on PDA medium with kresoxim-methyl and trifloxistrobin was monitored and sensitivity parameters (EC50 values) were determined using probit analysis. The isolates sensitivity in vivo was tested on apple seedlings using inoculation method. The EC50 values were ranging from 0.066-2.033 and 0.011-0.323 mu g mL(-1) for kresoxim-methyl and trifloxistrobin, respectively. The wild type of isolates showed a normal susceptibility to kresoxim-methyl and trifloxistrobin, while isolates originated from experimental and commercial orchard where those fungicides had been used extensively, showed significantly lower sensitivity to the both strobilurin fungicides. The isolate that showed the resistance to kresoxim-methyl and trifloxistrobin in vitro test, also showed reduced sensitivity in vivo test and could not been controlled satisfactory by fungicide concentrations that are commercially used, as well as by double higher, after apple seedlings inoculation. PB - Taylor & Francis Inc, Philadelphia T2 - Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Was T1 - Different toxicity of the strobilurin fungicides kresoxim-methyl and trifloxistrobin to Venturia inaequalis isolates from Serbia EP - 637 IS - 9 SP - 633 VL - 50 DO - 10.1080/03601234.2015.1038952 ER -
@article{ author = "Stević, Milan and Tamaš, Nenad and Miletić, Novica and Vukša, Petar", year = "2015", abstract = "Sensitivity of monosporial isolates of V. inaequalis, originated from apple orchards previously exposed (commercial and experimental) and those originated from location where fungicides had never been used (wild type), to strobilurin fungicides kresoxim-methyl and trifloxistrobin was investigated in this study. The experiments were carried out in laboratory using in vitro and in vivo methods. Mycelia growth inhibition on PDA medium with kresoxim-methyl and trifloxistrobin was monitored and sensitivity parameters (EC50 values) were determined using probit analysis. The isolates sensitivity in vivo was tested on apple seedlings using inoculation method. The EC50 values were ranging from 0.066-2.033 and 0.011-0.323 mu g mL(-1) for kresoxim-methyl and trifloxistrobin, respectively. The wild type of isolates showed a normal susceptibility to kresoxim-methyl and trifloxistrobin, while isolates originated from experimental and commercial orchard where those fungicides had been used extensively, showed significantly lower sensitivity to the both strobilurin fungicides. The isolate that showed the resistance to kresoxim-methyl and trifloxistrobin in vitro test, also showed reduced sensitivity in vivo test and could not been controlled satisfactory by fungicide concentrations that are commercially used, as well as by double higher, after apple seedlings inoculation.", publisher = "Taylor & Francis Inc, Philadelphia", journal = "Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Was", title = "Different toxicity of the strobilurin fungicides kresoxim-methyl and trifloxistrobin to Venturia inaequalis isolates from Serbia", pages = "637-633", number = "9", volume = "50", doi = "10.1080/03601234.2015.1038952" }
Stević, M., Tamaš, N., Miletić, N.,& Vukša, P.. (2015). Different toxicity of the strobilurin fungicides kresoxim-methyl and trifloxistrobin to Venturia inaequalis isolates from Serbia. in Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Was Taylor & Francis Inc, Philadelphia., 50(9), 633-637. https://doi.org/10.1080/03601234.2015.1038952
Stević M, Tamaš N, Miletić N, Vukša P. Different toxicity of the strobilurin fungicides kresoxim-methyl and trifloxistrobin to Venturia inaequalis isolates from Serbia. in Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Was. 2015;50(9):633-637. doi:10.1080/03601234.2015.1038952 .
Stević, Milan, Tamaš, Nenad, Miletić, Novica, Vukša, Petar, "Different toxicity of the strobilurin fungicides kresoxim-methyl and trifloxistrobin to Venturia inaequalis isolates from Serbia" in Journal of Environmental Science and Health Part B-Pesticides Food Contaminants and Agricultural Was, 50, no. 9 (2015):633-637, https://doi.org/10.1080/03601234.2015.1038952 . .