Agrobacterium arsernijevicii sp nov., isolated from crown gall tumors on raspberry and cherry plum
Само за регистроване кориснике
2015
Аутори
Kuzmanović, NemanjaPulawska, Joanna
Prokić, Andjelka
Ivanović, Milan
Zlatković, Nevena
Jones, Jeffrey B.
Obradović, Aleksa
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Two plant-tumorigenic strains KFB 330(T) and KFB 335 isolated from galls on raspberry (Rubus idaeus) in Serbia, and a non-pathogenic strain AL51.1 recovered from a cherry plum (Prunus cerasifera) tumor in Poland, were genotypically and phenotypically characterized. Phylogenetic reconstruction based on 16S rDNA placed them within the genus Agrobacterium, with A nepotum as their closest relative. Multilocus sequence analysis (MLSA) based on the partial sequences of atpD,glnA, gyrB, recA and rpoB housekeeping genes suggested that these three strains represent a new Agrobacterium species, that clustered with type strains of A. nepotum, A. radiobacter, "A fabrum" and A. pusense. This was further supported by average nucleotide identity values ( lt 92%) between the whole genome sequences of strain KFB 3301 and related Agrobacterium species. The major cellular fatty acids of the novel strains were 18:1 w7c (72.8-77.87%) and 16:0 (6.82-8.58%). Phenotypic features allowed their differentiation ...from closely related species. Polyphasic characterization showed that the three strains represent a novel species of the genus Agrobacterium, for which the name Agrobacterium arsenijevicii sp. nov. is proposed. The type strain ofA. arsenijevicii is KFB 330T (=CFBP 8308(T) = LMG 28674(T)).
Кључне речи:
Agrobacterium / Crown gall / 16S rDNA / MLSA / ANI / TaxonomyИзвор:
Systematic and Applied Microbiology, 2015, 38, 6, 373-378Издавач:
- Elsevier Gmbh, Munich
Финансирање / пројекти:
- Развој интегрисаних система управљања штетним организмима у биљној производњи са циљем превазилажења резистентности и унапређења квалитета и безбедности хране (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46008)
- Advancing research in agricultural and food sciences at Faculty of Agriculture, University of Belgrade (EU-FP7-316004)
- National Science Centre, PolandNational Science Center, PolandNational Science Centre, Poland [DEC-2013/08/M/NZ9/00138]
DOI: 10.1016/j.syapm.2015.06.001
ISSN: 0723-2020
PubMed: 26117193
WoS: 000368557400001
Scopus: 2-s2.0-84941260176
Институција/група
Poljoprivredni fakultetTY - JOUR AU - Kuzmanović, Nemanja AU - Pulawska, Joanna AU - Prokić, Andjelka AU - Ivanović, Milan AU - Zlatković, Nevena AU - Jones, Jeffrey B. AU - Obradović, Aleksa PY - 2015 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/3716 AB - Two plant-tumorigenic strains KFB 330(T) and KFB 335 isolated from galls on raspberry (Rubus idaeus) in Serbia, and a non-pathogenic strain AL51.1 recovered from a cherry plum (Prunus cerasifera) tumor in Poland, were genotypically and phenotypically characterized. Phylogenetic reconstruction based on 16S rDNA placed them within the genus Agrobacterium, with A nepotum as their closest relative. Multilocus sequence analysis (MLSA) based on the partial sequences of atpD,glnA, gyrB, recA and rpoB housekeeping genes suggested that these three strains represent a new Agrobacterium species, that clustered with type strains of A. nepotum, A. radiobacter, "A fabrum" and A. pusense. This was further supported by average nucleotide identity values ( lt 92%) between the whole genome sequences of strain KFB 3301 and related Agrobacterium species. The major cellular fatty acids of the novel strains were 18:1 w7c (72.8-77.87%) and 16:0 (6.82-8.58%). Phenotypic features allowed their differentiation from closely related species. Polyphasic characterization showed that the three strains represent a novel species of the genus Agrobacterium, for which the name Agrobacterium arsenijevicii sp. nov. is proposed. The type strain ofA. arsenijevicii is KFB 330T (=CFBP 8308(T) = LMG 28674(T)). PB - Elsevier Gmbh, Munich T2 - Systematic and Applied Microbiology T1 - Agrobacterium arsernijevicii sp nov., isolated from crown gall tumors on raspberry and cherry plum EP - 378 IS - 6 SP - 373 VL - 38 DO - 10.1016/j.syapm.2015.06.001 ER -
@article{ author = "Kuzmanović, Nemanja and Pulawska, Joanna and Prokić, Andjelka and Ivanović, Milan and Zlatković, Nevena and Jones, Jeffrey B. and Obradović, Aleksa", year = "2015", abstract = "Two plant-tumorigenic strains KFB 330(T) and KFB 335 isolated from galls on raspberry (Rubus idaeus) in Serbia, and a non-pathogenic strain AL51.1 recovered from a cherry plum (Prunus cerasifera) tumor in Poland, were genotypically and phenotypically characterized. Phylogenetic reconstruction based on 16S rDNA placed them within the genus Agrobacterium, with A nepotum as their closest relative. Multilocus sequence analysis (MLSA) based on the partial sequences of atpD,glnA, gyrB, recA and rpoB housekeeping genes suggested that these three strains represent a new Agrobacterium species, that clustered with type strains of A. nepotum, A. radiobacter, "A fabrum" and A. pusense. This was further supported by average nucleotide identity values ( lt 92%) between the whole genome sequences of strain KFB 3301 and related Agrobacterium species. The major cellular fatty acids of the novel strains were 18:1 w7c (72.8-77.87%) and 16:0 (6.82-8.58%). Phenotypic features allowed their differentiation from closely related species. Polyphasic characterization showed that the three strains represent a novel species of the genus Agrobacterium, for which the name Agrobacterium arsenijevicii sp. nov. is proposed. The type strain ofA. arsenijevicii is KFB 330T (=CFBP 8308(T) = LMG 28674(T)).", publisher = "Elsevier Gmbh, Munich", journal = "Systematic and Applied Microbiology", title = "Agrobacterium arsernijevicii sp nov., isolated from crown gall tumors on raspberry and cherry plum", pages = "378-373", number = "6", volume = "38", doi = "10.1016/j.syapm.2015.06.001" }
Kuzmanović, N., Pulawska, J., Prokić, A., Ivanović, M., Zlatković, N., Jones, J. B.,& Obradović, A.. (2015). Agrobacterium arsernijevicii sp nov., isolated from crown gall tumors on raspberry and cherry plum. in Systematic and Applied Microbiology Elsevier Gmbh, Munich., 38(6), 373-378. https://doi.org/10.1016/j.syapm.2015.06.001
Kuzmanović N, Pulawska J, Prokić A, Ivanović M, Zlatković N, Jones JB, Obradović A. Agrobacterium arsernijevicii sp nov., isolated from crown gall tumors on raspberry and cherry plum. in Systematic and Applied Microbiology. 2015;38(6):373-378. doi:10.1016/j.syapm.2015.06.001 .
Kuzmanović, Nemanja, Pulawska, Joanna, Prokić, Andjelka, Ivanović, Milan, Zlatković, Nevena, Jones, Jeffrey B., Obradović, Aleksa, "Agrobacterium arsernijevicii sp nov., isolated from crown gall tumors on raspberry and cherry plum" in Systematic and Applied Microbiology, 38, no. 6 (2015):373-378, https://doi.org/10.1016/j.syapm.2015.06.001 . .