Expression of bacteriocin LsbB is dependent on a transcription terminator
Apstrakt
The production of LsbB, leaderless class II bacteriocin, is encoded by genes (lsbB and lmrB) located on plasmid pMN5 in Lactococcus lactis BGMN1-5. Heterologous expression of the lsbB gene using the pAZIL vector (pAZIL-lsbB) in L. lactis subsp. cremoris MG7284 resulted in a significant reduction (more than 30 times) of bacteriocin LsbB expression. Subcloning and deletion experiments with plasmid pMN5 revealed that full expression of LsbB requires the presence of a complete transcription terminator located downstream of the lsbB gene. RNA stability analysis revealed that the presence of a transcription terminator increased the RNA stability by three times and the expression of LsbB by 30 times. The study of the influence of transcription terminator on the expression of other bacteriocin genes (lcnB, for lactococcin B production) indicated that this translational terminator likely functions in a lsbB-specific manner rather than in a general manner.
Ključne reči:
LsbB baceriocin / Transcription terminator regulation / Bacteriocin expressionIzvor:
Microbiological Research, 2015, 179, 45-53Izdavač:
- Elsevier Gmbh, Urban & Fischer Verlag, Jena
Finansiranje / projekti:
- Izučavanje gena i molekularnih mehanizama u osnovi probiotičke aktivnosti bakterija mlečne kiseline izolovanih sa područja zapadnog Balkana (RS-MESTD-Basic Research (BR or ON)-173019)
DOI: 10.1016/j.micres.2015.06.011
ISSN: 0944-5013
PubMed: 26411894
WoS: 000362923100006
Scopus: 2-s2.0-84942091660
Institucija/grupa
Poljoprivredni fakultetTY - JOUR AU - Uzelac, Gordana AU - Miljković, Marija AU - Lozo, Jelena AU - Radulović, Zorica AU - Tosić, Nataša AU - Kojić, Milan PY - 2015 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/3689 AB - The production of LsbB, leaderless class II bacteriocin, is encoded by genes (lsbB and lmrB) located on plasmid pMN5 in Lactococcus lactis BGMN1-5. Heterologous expression of the lsbB gene using the pAZIL vector (pAZIL-lsbB) in L. lactis subsp. cremoris MG7284 resulted in a significant reduction (more than 30 times) of bacteriocin LsbB expression. Subcloning and deletion experiments with plasmid pMN5 revealed that full expression of LsbB requires the presence of a complete transcription terminator located downstream of the lsbB gene. RNA stability analysis revealed that the presence of a transcription terminator increased the RNA stability by three times and the expression of LsbB by 30 times. The study of the influence of transcription terminator on the expression of other bacteriocin genes (lcnB, for lactococcin B production) indicated that this translational terminator likely functions in a lsbB-specific manner rather than in a general manner. PB - Elsevier Gmbh, Urban & Fischer Verlag, Jena T2 - Microbiological Research T1 - Expression of bacteriocin LsbB is dependent on a transcription terminator EP - 53 SP - 45 VL - 179 DO - 10.1016/j.micres.2015.06.011 ER -
@article{ author = "Uzelac, Gordana and Miljković, Marija and Lozo, Jelena and Radulović, Zorica and Tosić, Nataša and Kojić, Milan", year = "2015", abstract = "The production of LsbB, leaderless class II bacteriocin, is encoded by genes (lsbB and lmrB) located on plasmid pMN5 in Lactococcus lactis BGMN1-5. Heterologous expression of the lsbB gene using the pAZIL vector (pAZIL-lsbB) in L. lactis subsp. cremoris MG7284 resulted in a significant reduction (more than 30 times) of bacteriocin LsbB expression. Subcloning and deletion experiments with plasmid pMN5 revealed that full expression of LsbB requires the presence of a complete transcription terminator located downstream of the lsbB gene. RNA stability analysis revealed that the presence of a transcription terminator increased the RNA stability by three times and the expression of LsbB by 30 times. The study of the influence of transcription terminator on the expression of other bacteriocin genes (lcnB, for lactococcin B production) indicated that this translational terminator likely functions in a lsbB-specific manner rather than in a general manner.", publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena", journal = "Microbiological Research", title = "Expression of bacteriocin LsbB is dependent on a transcription terminator", pages = "53-45", volume = "179", doi = "10.1016/j.micres.2015.06.011" }
Uzelac, G., Miljković, M., Lozo, J., Radulović, Z., Tosić, N.,& Kojić, M.. (2015). Expression of bacteriocin LsbB is dependent on a transcription terminator. in Microbiological Research Elsevier Gmbh, Urban & Fischer Verlag, Jena., 179, 45-53. https://doi.org/10.1016/j.micres.2015.06.011
Uzelac G, Miljković M, Lozo J, Radulović Z, Tosić N, Kojić M. Expression of bacteriocin LsbB is dependent on a transcription terminator. in Microbiological Research. 2015;179:45-53. doi:10.1016/j.micres.2015.06.011 .
Uzelac, Gordana, Miljković, Marija, Lozo, Jelena, Radulović, Zorica, Tosić, Nataša, Kojić, Milan, "Expression of bacteriocin LsbB is dependent on a transcription terminator" in Microbiological Research, 179 (2015):45-53, https://doi.org/10.1016/j.micres.2015.06.011 . .