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Širenje virusa mozaika lucerke u usevu lavande u Hrvatskoj

dc.creatorStanković, Ivana
dc.creatorVrandečić, Karolina
dc.creatorĆosić, Jasenka
dc.creatorMilojević, Katarina
dc.creatorBulajić, Aleksandra
dc.creatorKrstić, Branka
dc.date.accessioned2020-12-17T21:09:38Z
dc.date.available2020-12-17T21:09:38Z
dc.date.issued2014
dc.identifier.issn1820-3949
dc.identifier.urihttp://aspace.agrif.bg.ac.rs/handle/123456789/3630
dc.description.abstractA survey was conducted in 2012 and 2013 to detect the presence and distribution of Alfalfa mosaic virus (AMV) in lavandin crops growing in continental parts of Croatia. A total of 73 lavandin samples from six crops in different localities were collected and analyzed for the presence of AMV and Cucumber mosaic virus (CMV) using commercial double-antibody sandwich (DAS)-ELISA kits. AMV was detected serologically in 62 samples collected at three different localities, and none of the samples tested positive for CMV. For further analyses, six selected samples of naturally infected lavandin plants originating from different localities were mechanically transmitted to test plants: Chenopodium quinoa, C. amaranticolor, Nicotiana benthamiana and Ocimum basilicum, confirming the infectious nature of the disease. Molecular detection was performed by amplification of a 751 bp fragment in all tested samples, using the specific primers CP AMV1/CP AMV2 that amplify the part of the coat protein (CP) gene and 3'-UTR. The RT-PCR products derived from the isolates 371-13 and 373-13 were sequenced (KJ504107 and KJ504108, respectively) and compared with the AMV sequences available in GenBank. CP sequence analysis, conducted using the MEGA5 software, revealed that the isolate 371-13 had the highest nucleotide identity of 99.5% (100% amino acid identity) with an isolate from Argentina originating from Medicago sativa (KC881010), while the sequence of isolate 373-13 had the highest identity with an Italian AMV isolate from Lavandula stoechas (FN667967) of 98.6% (99% amino acid identity). Phylogenetic analysis revealed the clustering of selected isolates into four molecular groups and the lavandin AMV isolates from Croatia grouped into two distinct groups, implying a significant variability within the AMV lavandin population.en
dc.description.abstractTokom 2012. i 2013. godine sprovedena su istraživanja u cilju utvrđivanja prisustva i rasprostranjenosti virusa mozaika lucerke (Alfalfa mosaic virus, AMV) u usevu lavande u kontinentalnom delu Hrvatske. Sa šest lokaliteta gajenja ukupno je sakupljeno 73 uzorka lavande koji su serološki testirani na prisustvo AMV i virusa mozaika krastavca (Cucumber mosaic virus, CMV) korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo AMV dokazano je u 62 uzorka lavande sakupljena sa tri različita lokaliteta, dok prisustvo CMV nije dokazano ni u jednom od testiranih uzoraka. Za dalja istraživanja odabrano je šest uzoraka prirodno zaraženih biljaka lavande poreklom iz različitih lokaliteta, koji su uspešno preneti mehaničkim inokulacijama na test biljke Chenopodium quinoa, C. amaranticolor, Nicotiana benthamiana, i Ocimum basilicum, čime je potvrđena infektivna priroda oboljenja. Molekularna detekcija obavljena je amplifikacijom fragmenta dužine 751 bp kod svih ispitivanih izolata korišćenjem specifičnih prajmera CP AMV1/CP AMV2 koji omogućavaju umnožavanje dela gena za proteinski omotač i 3' neprepisujućeg regiona. U cilju dalje identifikacije, RT-PCR produkti izolata 371-13 i 373-13 su sekvencionisani (KJ504107 i KJ504108) i upoređeni sa AMV sekvencama dostupnim u GenBank bazi podataka. Analizom sekvenci gena za protein omotača, korišćenjem MEGA5 softverskog paketa, najviši stepen nukleotidne sličnosti od 99.7% (100% aminokiselinska sličnost) izolat 371-13 pokazao je sa izolatom AMV iz Argentine poreklom iz Medicago sativa (KC881010), dok sekvenca izolata 373-13 najveću sličnost od 98.6% (99% aminokiselinska sličnost) deli sa italijanskim izolatom AMV iz Lavandula stoechas (FN667967). Filogenetska analiza pokazala je grupisanje izolata u četiri molekularne grupe, a izolati AMV iz lavande iz Hrvatske grupišu se u dve odvojene grupe, ukazujući na postojanje značajne varijabilnosti u populaciji AMV poreklom iz lavande.sr
dc.publisherInstitut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43001/RS//
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/316004/EU//
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-sa/4.0/
dc.sourcePesticidi i fitomedicina
dc.subjectAlfalfa mosaic virusen
dc.subjectLavandinen
dc.subjectMolecular detectionen
dc.subjectPhylogenetic analysisen
dc.subjectCroatiaen
dc.subjectVirus mozaika lucerkesr
dc.subjectlavandasr
dc.subjectmolekularna detekcijasr
dc.subjectfilogenetska analizasr
dc.subjectHrvatskasr
dc.titleThe spreading of alfalfa mosaic virus in lavandin in Croatiaen
dc.titleŠirenje virusa mozaika lucerke u usevu lavande u Hrvatskojsr
dc.typearticle
dc.rights.licenseBY-SA
dc.citation.epage122
dc.citation.issue2
dc.citation.other29(2): 115-122
dc.citation.rankM51
dc.citation.spage115
dc.citation.volume29
dc.identifier.doi10.2298/PIF1402115S
dc.identifier.fulltexthttp://aspace.agrif.bg.ac.rs/bitstream/id/2209/3627.pdf
dc.identifier.rcubconv_2318
dc.type.versionpublishedVersion


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