Primary proteolysis of white brined cheese prepared from raw cow milk monitored by high-molarity Tris buffer SDS-PAGE system
Апстракт
The aim of this work was to investigate primary proteolysis of white brined cow cheese prepared from raw milk by SDS-electrophoretic method based on high-molarity Tris buffer system and to correlate with the results of other commonly used parameters. Proteolytic changes of white brined cow cheese were monitored by three parameters: total protein, water soluble proteins and degree of proteolysis. Changes of major casein fractions were followed by SDS-electrophoretic system in reducing conditions. The total protein content and moisture content of white brined cow cheese were significantly affected by ripening. Ripening in brine increased water soluble proteins and degree of proteolysis. Major caseins were differently resistant to proteolysis; alpha(s)-CN was more susceptible than beta-CN. The alpha(s)-CN content was highly and negatively correlated with time of ripening and water soluble proteins whereas no significant correlation (p lt 0.05) between beta-CN content and these parameters ...was found. Also, a strong significant correlation (p lt 0.05) between the amount of low molecular weight products and time of ripening, water soluble proteins and alpha(s)-CN content was observed. SDS-PAGE method used in this study could be useful for monitoring the white cow cheese proteolysis.
Кључне речи:
proteolysis / SDS-PAGE / cow cheeseИзвор:
Mljekarstvo, 2014, 64, 2, 102-110Издавач:
- Hrvatska Mljekarska Udruga
Финансирање / пројекти:
- Унапређење и развој хигијенских и технолошких поступака у производњи намирница животињског порекла у циљу добијања квалитетних и безбедних производа конкурентних на светском тржишту (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46009)
Институција/група
Poljoprivredni fakultetTY - JOUR AU - Smiljanić, Milenko AU - Pešić, Mirjana AU - Stanojević, Sladjana AU - Barać, Miroljub PY - 2014 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/3518 AB - The aim of this work was to investigate primary proteolysis of white brined cow cheese prepared from raw milk by SDS-electrophoretic method based on high-molarity Tris buffer system and to correlate with the results of other commonly used parameters. Proteolytic changes of white brined cow cheese were monitored by three parameters: total protein, water soluble proteins and degree of proteolysis. Changes of major casein fractions were followed by SDS-electrophoretic system in reducing conditions. The total protein content and moisture content of white brined cow cheese were significantly affected by ripening. Ripening in brine increased water soluble proteins and degree of proteolysis. Major caseins were differently resistant to proteolysis; alpha(s)-CN was more susceptible than beta-CN. The alpha(s)-CN content was highly and negatively correlated with time of ripening and water soluble proteins whereas no significant correlation (p lt 0.05) between beta-CN content and these parameters was found. Also, a strong significant correlation (p lt 0.05) between the amount of low molecular weight products and time of ripening, water soluble proteins and alpha(s)-CN content was observed. SDS-PAGE method used in this study could be useful for monitoring the white cow cheese proteolysis. PB - Hrvatska Mljekarska Udruga T2 - Mljekarstvo T1 - Primary proteolysis of white brined cheese prepared from raw cow milk monitored by high-molarity Tris buffer SDS-PAGE system EP - 110 IS - 2 SP - 102 VL - 64 UR - https://hdl.handle.net/21.15107/rcub_agrospace_3518 ER -
@article{ author = "Smiljanić, Milenko and Pešić, Mirjana and Stanojević, Sladjana and Barać, Miroljub", year = "2014", abstract = "The aim of this work was to investigate primary proteolysis of white brined cow cheese prepared from raw milk by SDS-electrophoretic method based on high-molarity Tris buffer system and to correlate with the results of other commonly used parameters. Proteolytic changes of white brined cow cheese were monitored by three parameters: total protein, water soluble proteins and degree of proteolysis. Changes of major casein fractions were followed by SDS-electrophoretic system in reducing conditions. The total protein content and moisture content of white brined cow cheese were significantly affected by ripening. Ripening in brine increased water soluble proteins and degree of proteolysis. Major caseins were differently resistant to proteolysis; alpha(s)-CN was more susceptible than beta-CN. The alpha(s)-CN content was highly and negatively correlated with time of ripening and water soluble proteins whereas no significant correlation (p lt 0.05) between beta-CN content and these parameters was found. Also, a strong significant correlation (p lt 0.05) between the amount of low molecular weight products and time of ripening, water soluble proteins and alpha(s)-CN content was observed. SDS-PAGE method used in this study could be useful for monitoring the white cow cheese proteolysis.", publisher = "Hrvatska Mljekarska Udruga", journal = "Mljekarstvo", title = "Primary proteolysis of white brined cheese prepared from raw cow milk monitored by high-molarity Tris buffer SDS-PAGE system", pages = "110-102", number = "2", volume = "64", url = "https://hdl.handle.net/21.15107/rcub_agrospace_3518" }
Smiljanić, M., Pešić, M., Stanojević, S.,& Barać, M.. (2014). Primary proteolysis of white brined cheese prepared from raw cow milk monitored by high-molarity Tris buffer SDS-PAGE system. in Mljekarstvo Hrvatska Mljekarska Udruga., 64(2), 102-110. https://hdl.handle.net/21.15107/rcub_agrospace_3518
Smiljanić M, Pešić M, Stanojević S, Barać M. Primary proteolysis of white brined cheese prepared from raw cow milk monitored by high-molarity Tris buffer SDS-PAGE system. in Mljekarstvo. 2014;64(2):102-110. https://hdl.handle.net/21.15107/rcub_agrospace_3518 .
Smiljanić, Milenko, Pešić, Mirjana, Stanojević, Sladjana, Barać, Miroljub, "Primary proteolysis of white brined cheese prepared from raw cow milk monitored by high-molarity Tris buffer SDS-PAGE system" in Mljekarstvo, 64, no. 2 (2014):102-110, https://hdl.handle.net/21.15107/rcub_agrospace_3518 .