Application possibilities of Raman spectroscopy in the investigation of milk and dairy products

Abstract

Raman spectroscopy is a vibrational spectroscopic technique based on inelastic light scattering. This non-destructive, non-contact technique provides a number of qualitative and quantitative information about the different samples and has been applied in many fields of research. In combination with an optical microscope this method is ideal for testing of heterogeneous systems. The limitations of this technique include problems with fluorescence, but nowadays they are significantly reduced with developed instrumental solutions. Thanks to the numerous advantages (very weak signal lead, working with concentrated solutions, a wide range of sample types, etc.), Raman spectroscopy has great potential for application in the examination of milk and milk products.

Raman spektroskopija je vibraciona spektroskopska tehnika koja se zasniva na neelastičnom rasejanju svetlosti. Ova nedestruktivna, bezkontaktna tehnika daje brojne kvalitativne i kvantitativne informacije o različitim uzorcima i našla je primenu u mnogim oblastima istraživanja. U kombinaciji sa optičkim mikroskopom idealna je za ispitivanje heterogenih sistema. Od početka primene, ograničenja ove tehnike tiču se problema sa fluorescencijom, koji su se razvojem instrumentalnih rešenja danas značajno smanjili. Zahvaljujući prednostima koje pruža (vrlo slab signal vode, rad sa koncentrovanim rastvorima, širok opseg tipova uzoraka i dr.), Raman spektroskopija ima vrlo veliki potencijal za primenu u ispitivanju mleka i proizvoda od mleka.