Electroporation Enhances the Metabolic Activity of Lactobacillus plantarum 564
Нема приказа
Аутори
Seratlić, Sanja V.Bugarski, Branko
Radulović, Zorica
Dejmek, Petr
Wadso, Lars
Nedović, Viktor
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The exposure of bacterial cells to pulsed electric fields (PEF) leads to the reversible formation of pores in the cell membrane if an applied energy is below the critical level. Therefore, the effect of electric field pulses with amplitudes below 14 kV/cm and the applied energy up to 12.2 J/cm(3) on the growth of Lactobacillus plantarum 564 cells was investigated. After PEF treatments, the growth of lactobacilli in De Man-Rogosa-Sharpe broth at 37 degrees C was monitored by isothermal calorimetry, absorbance and plate counts. All the applied treatments resulted in a higher growth rate of PEF-treated cells during early and mid-log phase, especially bacterial samples treated with lower field intensities (1.3-5.5 J/cm(3)). The transport of ions and molecules through the cell membrane (which facilitates the growth of electroporated lactobacilli) was particularly evident in the mid-exponential growth phase, where the doubling time was reduced more than 3 times after the exposure to electric... pulses of 5.5 Yore. The heat production rate during the growth of electroporated cells was also higher, indicating the enhanced metabolic activity of PEF-treated cells. Moreover, the electroporated cells had a better acidification ability than the untreated ones. It can be summarized that the applied PEF treatments with an energy input of below 12 J/cm(3) potentially induce reversible electroporation of the cell membrane, which has a positive impact on the growth and metabolic activity of the cells of lactobacilli.
Кључне речи:
Lactobacillus plantarum / pulsed electric fields / isothermal calorimetryИзвор:
Food Technology and Biotechnology, 2013, 51, 4, 446-452Издавач:
- University of Zagreb
Финансирање / пројекти:
- BASI-LEUS
- Развој нових инкапсулационих и ензимских технологија за производњу биокатализатора и биолошки активних компонената хране у циљу повећања њене конкурентности, квалитета и безбедности (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
Институција/група
Poljoprivredni fakultetTY - JOUR AU - Seratlić, Sanja V. AU - Bugarski, Branko AU - Radulović, Zorica AU - Dejmek, Petr AU - Wadso, Lars AU - Nedović, Viktor PY - 2013 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/3155 AB - The exposure of bacterial cells to pulsed electric fields (PEF) leads to the reversible formation of pores in the cell membrane if an applied energy is below the critical level. Therefore, the effect of electric field pulses with amplitudes below 14 kV/cm and the applied energy up to 12.2 J/cm(3) on the growth of Lactobacillus plantarum 564 cells was investigated. After PEF treatments, the growth of lactobacilli in De Man-Rogosa-Sharpe broth at 37 degrees C was monitored by isothermal calorimetry, absorbance and plate counts. All the applied treatments resulted in a higher growth rate of PEF-treated cells during early and mid-log phase, especially bacterial samples treated with lower field intensities (1.3-5.5 J/cm(3)). The transport of ions and molecules through the cell membrane (which facilitates the growth of electroporated lactobacilli) was particularly evident in the mid-exponential growth phase, where the doubling time was reduced more than 3 times after the exposure to electric pulses of 5.5 Yore. The heat production rate during the growth of electroporated cells was also higher, indicating the enhanced metabolic activity of PEF-treated cells. Moreover, the electroporated cells had a better acidification ability than the untreated ones. It can be summarized that the applied PEF treatments with an energy input of below 12 J/cm(3) potentially induce reversible electroporation of the cell membrane, which has a positive impact on the growth and metabolic activity of the cells of lactobacilli. PB - University of Zagreb T2 - Food Technology and Biotechnology T1 - Electroporation Enhances the Metabolic Activity of Lactobacillus plantarum 564 EP - 452 IS - 4 SP - 446 VL - 51 UR - https://hdl.handle.net/21.15107/rcub_agrospace_3155 ER -
@article{ author = "Seratlić, Sanja V. and Bugarski, Branko and Radulović, Zorica and Dejmek, Petr and Wadso, Lars and Nedović, Viktor", year = "2013", abstract = "The exposure of bacterial cells to pulsed electric fields (PEF) leads to the reversible formation of pores in the cell membrane if an applied energy is below the critical level. Therefore, the effect of electric field pulses with amplitudes below 14 kV/cm and the applied energy up to 12.2 J/cm(3) on the growth of Lactobacillus plantarum 564 cells was investigated. After PEF treatments, the growth of lactobacilli in De Man-Rogosa-Sharpe broth at 37 degrees C was monitored by isothermal calorimetry, absorbance and plate counts. All the applied treatments resulted in a higher growth rate of PEF-treated cells during early and mid-log phase, especially bacterial samples treated with lower field intensities (1.3-5.5 J/cm(3)). The transport of ions and molecules through the cell membrane (which facilitates the growth of electroporated lactobacilli) was particularly evident in the mid-exponential growth phase, where the doubling time was reduced more than 3 times after the exposure to electric pulses of 5.5 Yore. The heat production rate during the growth of electroporated cells was also higher, indicating the enhanced metabolic activity of PEF-treated cells. Moreover, the electroporated cells had a better acidification ability than the untreated ones. It can be summarized that the applied PEF treatments with an energy input of below 12 J/cm(3) potentially induce reversible electroporation of the cell membrane, which has a positive impact on the growth and metabolic activity of the cells of lactobacilli.", publisher = "University of Zagreb", journal = "Food Technology and Biotechnology", title = "Electroporation Enhances the Metabolic Activity of Lactobacillus plantarum 564", pages = "452-446", number = "4", volume = "51", url = "https://hdl.handle.net/21.15107/rcub_agrospace_3155" }
Seratlić, S. V., Bugarski, B., Radulović, Z., Dejmek, P., Wadso, L.,& Nedović, V.. (2013). Electroporation Enhances the Metabolic Activity of Lactobacillus plantarum 564. in Food Technology and Biotechnology University of Zagreb., 51(4), 446-452. https://hdl.handle.net/21.15107/rcub_agrospace_3155
Seratlić SV, Bugarski B, Radulović Z, Dejmek P, Wadso L, Nedović V. Electroporation Enhances the Metabolic Activity of Lactobacillus plantarum 564. in Food Technology and Biotechnology. 2013;51(4):446-452. https://hdl.handle.net/21.15107/rcub_agrospace_3155 .
Seratlić, Sanja V., Bugarski, Branko, Radulović, Zorica, Dejmek, Petr, Wadso, Lars, Nedović, Viktor, "Electroporation Enhances the Metabolic Activity of Lactobacillus plantarum 564" in Food Technology and Biotechnology, 51, no. 4 (2013):446-452, https://hdl.handle.net/21.15107/rcub_agrospace_3155 .