Presence and characterization of Zucchini yellow mosaic virus in watermelon in Serbia
Prisustvo i karakterizacija virusa žutog mozaika cukinija u usevu lubenice u Srbiji
2012
Authors
Vučurović, AnaBulajić, Aleksandra
Milojević, Katarina
Stanković, Ivana
Ristić, Danijela
Berenji, Janoš
Krstić, Branka
Article (Published version)
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The presence of Zucchini yellow mosaic virus (ZYMV) in two out of seven watermelon production localities in Serbia during 2011 was investigated by analyzing leaves sampled from symptomatic and asymptomatic watermelon plants and utilizing DAS-ELISA test. In the locality of Gornji Tavankut, ZYMV was detected in 23.08% of tested plants in single infections, and in the locality of Silbas it was detected in 35.29% of tested plants in mixed infections with Cucumber mosaic virus and Alfalfa mosaic virus. ZYMV was successfully mechanically transmitted from naturally infected watermelon plants to Cucurbita pepo 'Ezra F1'. Molecular detection was performed by RT-PCR and amplification of part of the gene for nuclear inclusions, gene of coat protein and part of 3' non-coding region, which confirmed the identification of the ZYMV isolates. Phylogenetic analysis revealed grouping of the isolate originating from watermelon with other isolates from Serbia and Central Europe within A-I subgroup. Analys...is of amino acid sequences of the N terminal end of the CP gene revealed that isolate 550-11 belongs to the Central European branch.
Prisustvo virusa žutog mozaika cukinija (Zucchini yellow mosaic virus, ZYMV) na dva od sedam lokaliteta gajenja lubenice u Srbiji tokom 2011. godine utvrđeno je analizom uzoraka lišća simptomatičnih i asimptomatičnih biljaka lubenice primenom DAS-ELISA metode. Na lokalitetu Gornji Tavankut, ZYMV je dokazan u 23,08% testiranih biljaka u pojedinačnim infekcijama, a na lokalitetu Silbaš u 35,29% testiranih biljaka u mešanim infekcijama sa virusom mozaika krastavca i virusom mozaika lubenice. ZYMV je uspešno mehanički prenesen sa prirodno zaraženih biljaka lubenice na Cucurbita pepo 'Ezra F1'. Molekularna detekcija obavljena je RT-PCR metodom umnožavanjem dela gena za nuklearne inkluzije, gena za protein omotača i dela 3' neprepisujućeg regiona, čime je potvrđena identifikacija izolata. Filogenetske analize pokazale su grupisanje izolata poreklom iz lubenice sa drugim izolatima iz Srbije i Centralne Evrope u okviru A-I podgrupe. Analize aminokiselinske sekvence N-terminalnog kraja CP gena,... takođe su pokazale pripadnost izolata 550-11 centralnoevropskoj grani.
Keywords:
isolates / watermelons / Zucchini yellow mosaic virus / izolati / lubenica / virus žutog mozaika cukinijaSource:
Ratarstvo i povrtarstvo, 2012, 49, 2, 151-159Publisher:
- Institut za ratarstvo i povrtarstvo, Novi Sad
Funding / projects:
- Agrobiodiversity and land-use change in Serbia: an integrated biodiversity assessment of key functional groups of arthropods and plant pathogens (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-43001)
- Development of new varieties and production technology improvement of oil crops for different purposes (RS-MESTD-Technological Development (TD or TR)-31025)
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Institution/Community
Poljoprivredni fakultetTY - JOUR AU - Vučurović, Ana AU - Bulajić, Aleksandra AU - Milojević, Katarina AU - Stanković, Ivana AU - Ristić, Danijela AU - Berenji, Janoš AU - Krstić, Branka PY - 2012 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/2935 AB - The presence of Zucchini yellow mosaic virus (ZYMV) in two out of seven watermelon production localities in Serbia during 2011 was investigated by analyzing leaves sampled from symptomatic and asymptomatic watermelon plants and utilizing DAS-ELISA test. In the locality of Gornji Tavankut, ZYMV was detected in 23.08% of tested plants in single infections, and in the locality of Silbas it was detected in 35.29% of tested plants in mixed infections with Cucumber mosaic virus and Alfalfa mosaic virus. ZYMV was successfully mechanically transmitted from naturally infected watermelon plants to Cucurbita pepo 'Ezra F1'. Molecular detection was performed by RT-PCR and amplification of part of the gene for nuclear inclusions, gene of coat protein and part of 3' non-coding region, which confirmed the identification of the ZYMV isolates. Phylogenetic analysis revealed grouping of the isolate originating from watermelon with other isolates from Serbia and Central Europe within A-I subgroup. Analysis of amino acid sequences of the N terminal end of the CP gene revealed that isolate 550-11 belongs to the Central European branch. AB - Prisustvo virusa žutog mozaika cukinija (Zucchini yellow mosaic virus, ZYMV) na dva od sedam lokaliteta gajenja lubenice u Srbiji tokom 2011. godine utvrđeno je analizom uzoraka lišća simptomatičnih i asimptomatičnih biljaka lubenice primenom DAS-ELISA metode. Na lokalitetu Gornji Tavankut, ZYMV je dokazan u 23,08% testiranih biljaka u pojedinačnim infekcijama, a na lokalitetu Silbaš u 35,29% testiranih biljaka u mešanim infekcijama sa virusom mozaika krastavca i virusom mozaika lubenice. ZYMV je uspešno mehanički prenesen sa prirodno zaraženih biljaka lubenice na Cucurbita pepo 'Ezra F1'. Molekularna detekcija obavljena je RT-PCR metodom umnožavanjem dela gena za nuklearne inkluzije, gena za protein omotača i dela 3' neprepisujućeg regiona, čime je potvrđena identifikacija izolata. Filogenetske analize pokazale su grupisanje izolata poreklom iz lubenice sa drugim izolatima iz Srbije i Centralne Evrope u okviru A-I podgrupe. Analize aminokiselinske sekvence N-terminalnog kraja CP gena, takođe su pokazale pripadnost izolata 550-11 centralnoevropskoj grani. PB - Institut za ratarstvo i povrtarstvo, Novi Sad T2 - Ratarstvo i povrtarstvo T1 - Presence and characterization of Zucchini yellow mosaic virus in watermelon in Serbia T1 - Prisustvo i karakterizacija virusa žutog mozaika cukinija u usevu lubenice u Srbiji EP - 159 IS - 2 SP - 151 VL - 49 DO - 10.5937/ratpov49-1773 ER -
@article{ author = "Vučurović, Ana and Bulajić, Aleksandra and Milojević, Katarina and Stanković, Ivana and Ristić, Danijela and Berenji, Janoš and Krstić, Branka", year = "2012", abstract = "The presence of Zucchini yellow mosaic virus (ZYMV) in two out of seven watermelon production localities in Serbia during 2011 was investigated by analyzing leaves sampled from symptomatic and asymptomatic watermelon plants and utilizing DAS-ELISA test. In the locality of Gornji Tavankut, ZYMV was detected in 23.08% of tested plants in single infections, and in the locality of Silbas it was detected in 35.29% of tested plants in mixed infections with Cucumber mosaic virus and Alfalfa mosaic virus. ZYMV was successfully mechanically transmitted from naturally infected watermelon plants to Cucurbita pepo 'Ezra F1'. Molecular detection was performed by RT-PCR and amplification of part of the gene for nuclear inclusions, gene of coat protein and part of 3' non-coding region, which confirmed the identification of the ZYMV isolates. Phylogenetic analysis revealed grouping of the isolate originating from watermelon with other isolates from Serbia and Central Europe within A-I subgroup. Analysis of amino acid sequences of the N terminal end of the CP gene revealed that isolate 550-11 belongs to the Central European branch., Prisustvo virusa žutog mozaika cukinija (Zucchini yellow mosaic virus, ZYMV) na dva od sedam lokaliteta gajenja lubenice u Srbiji tokom 2011. godine utvrđeno je analizom uzoraka lišća simptomatičnih i asimptomatičnih biljaka lubenice primenom DAS-ELISA metode. Na lokalitetu Gornji Tavankut, ZYMV je dokazan u 23,08% testiranih biljaka u pojedinačnim infekcijama, a na lokalitetu Silbaš u 35,29% testiranih biljaka u mešanim infekcijama sa virusom mozaika krastavca i virusom mozaika lubenice. ZYMV je uspešno mehanički prenesen sa prirodno zaraženih biljaka lubenice na Cucurbita pepo 'Ezra F1'. Molekularna detekcija obavljena je RT-PCR metodom umnožavanjem dela gena za nuklearne inkluzije, gena za protein omotača i dela 3' neprepisujućeg regiona, čime je potvrđena identifikacija izolata. Filogenetske analize pokazale su grupisanje izolata poreklom iz lubenice sa drugim izolatima iz Srbije i Centralne Evrope u okviru A-I podgrupe. Analize aminokiselinske sekvence N-terminalnog kraja CP gena, takođe su pokazale pripadnost izolata 550-11 centralnoevropskoj grani.", publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad", journal = "Ratarstvo i povrtarstvo", title = "Presence and characterization of Zucchini yellow mosaic virus in watermelon in Serbia, Prisustvo i karakterizacija virusa žutog mozaika cukinija u usevu lubenice u Srbiji", pages = "159-151", number = "2", volume = "49", doi = "10.5937/ratpov49-1773" }
Vučurović, A., Bulajić, A., Milojević, K., Stanković, I., Ristić, D., Berenji, J.,& Krstić, B.. (2012). Presence and characterization of Zucchini yellow mosaic virus in watermelon in Serbia. in Ratarstvo i povrtarstvo Institut za ratarstvo i povrtarstvo, Novi Sad., 49(2), 151-159. https://doi.org/10.5937/ratpov49-1773
Vučurović A, Bulajić A, Milojević K, Stanković I, Ristić D, Berenji J, Krstić B. Presence and characterization of Zucchini yellow mosaic virus in watermelon in Serbia. in Ratarstvo i povrtarstvo. 2012;49(2):151-159. doi:10.5937/ratpov49-1773 .
Vučurović, Ana, Bulajić, Aleksandra, Milojević, Katarina, Stanković, Ivana, Ristić, Danijela, Berenji, Janoš, Krstić, Branka, "Presence and characterization of Zucchini yellow mosaic virus in watermelon in Serbia" in Ratarstvo i povrtarstvo, 49, no. 2 (2012):151-159, https://doi.org/10.5937/ratpov49-1773 . .