A Comparative Study of DNA Binding and Cell Cycle Phase Perturbation by the Dinuclear Complex of Cd(II) with the Condensation Product of 2-Acetylpyridine and Malonic Acid Dihydrazide N ',N ' 2-bis[(1E)-1-(2-pyridyl) ethylidene] propanedihydrazide
Само за регистроване кориснике
2011
Аутори
Vujcić, MiroslavaLazić, Milan
Milenković, Milica
Sladić, Dušan M.
Radulović, Siniša
Filipović, Nenad
Andjelković, Katarina
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Organometallic Cd(II) compounds have recently attracted attention for their anticancer activity. The interaction of the dinuclear complex of Cd(II) with the condensation product of 2-acetylpyridine and malonic acid dihydrazide, N',N'(2)-bis[(1E)-1-(2pyridyl) ethylidene] propanedihydrazide (Cd(II)H(2)L), with calf thymus DNA (CT-DNA) was monitored by blue shift in UV-vis spectra of the complex. The binding constant of Cd(II) H2L complex with CT-DNA was determined (K(B) = 1.8 x 10(4) M(-1)) and was indicative of minor groove binding. Agarose gel electrophoretic changes in mobility of supercoiled and circular forms of pBR322 and pUC18 plasmids in the presence of the complex suggest that conformational changes in the plasmids occur upon binding of the Cd(II) H2L complex. The Cd(II) H2L complex induced perturbation of the cell cycle phase distribution and an increase in the percentage of cells in the sub-G1 phase of human cervical cancer HeLa cell line and murine melanoma B16 cell line. Imm...unoblotting analysis showed the overexpression of Bcl-2 protein with the Cd(II)H(2)L complex.
Кључне речи:
DNA Binding / Electrophoretic Analysis / Cell Cycle Phase PerturbationИзвор:
Journal of Biochemical and Molecular Toxicology, 2011, 25, 3, 175-182Издавач:
- Wiley-Blackwell, Malden
Финансирање / пројекти:
- Интеракције природних производа и њихових аналога са протеинима и нуклеинским киселинама (RS-MESTD-MPN2006-2010-142026)
- Фармакодинамска и фармакогенетска истраживања нових лекова и предиктивна/прогностичка вредност фармакотерапије у онкологији (RS-MESTD-MPN2006-2010-145035)
DOI: 10.1002/jbt.20374
ISSN: 1095-6670
PubMed: 21671309
WoS: 000291562100007
Scopus: 2-s2.0-79958856469
Институција/група
Poljoprivredni fakultetTY - JOUR AU - Vujcić, Miroslava AU - Lazić, Milan AU - Milenković, Milica AU - Sladić, Dušan M. AU - Radulović, Siniša AU - Filipović, Nenad AU - Andjelković, Katarina PY - 2011 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/2641 AB - Organometallic Cd(II) compounds have recently attracted attention for their anticancer activity. The interaction of the dinuclear complex of Cd(II) with the condensation product of 2-acetylpyridine and malonic acid dihydrazide, N',N'(2)-bis[(1E)-1-(2pyridyl) ethylidene] propanedihydrazide (Cd(II)H(2)L), with calf thymus DNA (CT-DNA) was monitored by blue shift in UV-vis spectra of the complex. The binding constant of Cd(II) H2L complex with CT-DNA was determined (K(B) = 1.8 x 10(4) M(-1)) and was indicative of minor groove binding. Agarose gel electrophoretic changes in mobility of supercoiled and circular forms of pBR322 and pUC18 plasmids in the presence of the complex suggest that conformational changes in the plasmids occur upon binding of the Cd(II) H2L complex. The Cd(II) H2L complex induced perturbation of the cell cycle phase distribution and an increase in the percentage of cells in the sub-G1 phase of human cervical cancer HeLa cell line and murine melanoma B16 cell line. Immunoblotting analysis showed the overexpression of Bcl-2 protein with the Cd(II)H(2)L complex. PB - Wiley-Blackwell, Malden T2 - Journal of Biochemical and Molecular Toxicology T1 - A Comparative Study of DNA Binding and Cell Cycle Phase Perturbation by the Dinuclear Complex of Cd(II) with the Condensation Product of 2-Acetylpyridine and Malonic Acid Dihydrazide N ',N ' 2-bis[(1E)-1-(2-pyridyl) ethylidene] propanedihydrazide EP - 182 IS - 3 SP - 175 VL - 25 DO - 10.1002/jbt.20374 ER -
@article{ author = "Vujcić, Miroslava and Lazić, Milan and Milenković, Milica and Sladić, Dušan M. and Radulović, Siniša and Filipović, Nenad and Andjelković, Katarina", year = "2011", abstract = "Organometallic Cd(II) compounds have recently attracted attention for their anticancer activity. The interaction of the dinuclear complex of Cd(II) with the condensation product of 2-acetylpyridine and malonic acid dihydrazide, N',N'(2)-bis[(1E)-1-(2pyridyl) ethylidene] propanedihydrazide (Cd(II)H(2)L), with calf thymus DNA (CT-DNA) was monitored by blue shift in UV-vis spectra of the complex. The binding constant of Cd(II) H2L complex with CT-DNA was determined (K(B) = 1.8 x 10(4) M(-1)) and was indicative of minor groove binding. Agarose gel electrophoretic changes in mobility of supercoiled and circular forms of pBR322 and pUC18 plasmids in the presence of the complex suggest that conformational changes in the plasmids occur upon binding of the Cd(II) H2L complex. The Cd(II) H2L complex induced perturbation of the cell cycle phase distribution and an increase in the percentage of cells in the sub-G1 phase of human cervical cancer HeLa cell line and murine melanoma B16 cell line. Immunoblotting analysis showed the overexpression of Bcl-2 protein with the Cd(II)H(2)L complex.", publisher = "Wiley-Blackwell, Malden", journal = "Journal of Biochemical and Molecular Toxicology", title = "A Comparative Study of DNA Binding and Cell Cycle Phase Perturbation by the Dinuclear Complex of Cd(II) with the Condensation Product of 2-Acetylpyridine and Malonic Acid Dihydrazide N ',N ' 2-bis[(1E)-1-(2-pyridyl) ethylidene] propanedihydrazide", pages = "182-175", number = "3", volume = "25", doi = "10.1002/jbt.20374" }
Vujcić, M., Lazić, M., Milenković, M., Sladić, D. M., Radulović, S., Filipović, N.,& Andjelković, K.. (2011). A Comparative Study of DNA Binding and Cell Cycle Phase Perturbation by the Dinuclear Complex of Cd(II) with the Condensation Product of 2-Acetylpyridine and Malonic Acid Dihydrazide N ',N ' 2-bis[(1E)-1-(2-pyridyl) ethylidene] propanedihydrazide. in Journal of Biochemical and Molecular Toxicology Wiley-Blackwell, Malden., 25(3), 175-182. https://doi.org/10.1002/jbt.20374
Vujcić M, Lazić M, Milenković M, Sladić DM, Radulović S, Filipović N, Andjelković K. A Comparative Study of DNA Binding and Cell Cycle Phase Perturbation by the Dinuclear Complex of Cd(II) with the Condensation Product of 2-Acetylpyridine and Malonic Acid Dihydrazide N ',N ' 2-bis[(1E)-1-(2-pyridyl) ethylidene] propanedihydrazide. in Journal of Biochemical and Molecular Toxicology. 2011;25(3):175-182. doi:10.1002/jbt.20374 .
Vujcić, Miroslava, Lazić, Milan, Milenković, Milica, Sladić, Dušan M., Radulović, Siniša, Filipović, Nenad, Andjelković, Katarina, "A Comparative Study of DNA Binding and Cell Cycle Phase Perturbation by the Dinuclear Complex of Cd(II) with the Condensation Product of 2-Acetylpyridine and Malonic Acid Dihydrazide N ',N ' 2-bis[(1E)-1-(2-pyridyl) ethylidene] propanedihydrazide" in Journal of Biochemical and Molecular Toxicology, 25, no. 3 (2011):175-182, https://doi.org/10.1002/jbt.20374 . .