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dc.creatorStanković, Ivana
dc.creatorBulajić, Aleksandra
dc.creatorVučurović, Ana
dc.creatorRistić, Danijela
dc.creatorMilojević, Katarina
dc.creatorBerenji, Janoš
dc.creatorKrstić, Branka
dc.date.accessioned2020-12-17T20:08:52Z
dc.date.available2020-12-17T20:08:52Z
dc.date.issued2011
dc.identifier.issn0001-723X
dc.identifier.urihttp://aspace.agrif.bg.ac.rs/handle/123456789/2614
dc.description.abstractIn a four-year survey to determine the presence and distribution of viruses in tobacco crops at 17 localities of the Vojvodina Province and Central Serbia, 380 samples were collected and analyzed by DAS-ELISA. Out of the seven viruses tested, tomato spotted wilt virus (TSWV), potato virus Y (PVY), tobacco mosaic virus (TMV), cucumber mosaic virus (CMV), and alfalfa mosaic virus (AMV) were detected in 37.9, 33.4, 28.7, 23.9, and 15.5% of the total tested samples, respectively. TSWV was the most frequently found virus at the localities of Central Serbia, while PVY and CMV were the most frequent viruses in the Vojvodina Province. Single infections were prevalent in years 2005-2007 and the most frequent were those of PVY A triple combination of those viruses was most frequent mixed infection type in 2008. The presence of all five detected viruses was confirmed in selected ELISA-positive samples by RT-PCR and sequencing. The comparisons of obtained virus isolate sequences with those available in NCBI, confirmed the authenticity of serologically detected viruses. Phylogenetic analysis based on partial nucleocapsid gene sequences revealed a joint clustering of Serbian, Bulgarian and Montenegrin TSWV isolates into one geographic subpopulation, which was distinct from the other subpopulation of TSWV isolates from the rest of the European countries. The high incidence of viruses in Serbian tobacco crops highlights the importance of enhancing farmers knowledge towards better implementation of control strategies for preventing serious losses.en
dc.publisherAepress Sro, Bratislava
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/43001/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31025/RS//
dc.rightsopenAccess
dc.sourceActa Virologica
dc.subjecttobaccoen
dc.subjectsurveyen
dc.subjectvirusesen
dc.subjectDAS-ELISAen
dc.subjectRT-PCRen
dc.subjectphylogenetic analysisen
dc.titleStatus of tobacco viruses in Serbia and molecular characterization of tomato spotted wilt virus isolatesen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage347
dc.citation.issue4
dc.citation.other55(4): 337-347
dc.citation.rankM23
dc.citation.spage337
dc.citation.volume55
dc.identifier.doi10.4149/av_2011_04_337
dc.identifier.fulltexthttp://aspace.agrif.bg.ac.rs/bitstream/id/1373/2611.pdf
dc.identifier.scopus2-s2.0-84855932944
dc.identifier.pmid22149499
dc.identifier.wos000302266100007
dc.type.versionpublishedVersion


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Приказ основних података о документу