Приказ основних података о документу

dc.creatorJurick, Wayne M., II
dc.creatorVico, Ivana
dc.creatorGaskins, Verneta L.
dc.creatorGarrett, Wesley M.
dc.creatorWhitaker, Bruce D.
dc.creatorJanisiewicz, Wojciech
dc.creatorConway, William S.
dc.date.accessioned2020-12-17T19:54:15Z
dc.date.available2020-12-17T19:54:15Z
dc.date.issued2010
dc.identifier.issn0031-949X
dc.identifier.urihttp://aspace.agrif.bg.ac.rs/handle/123456789/2375
dc.description.abstractA polygalacturonase (PG) was extracted and purified from decayed tissue of 'Anjou' pear fruit inoculated with Penicillium expansum. Ammonium sulfate precipitation, gel filtration, and cation exchange chromatography were used to purify the enzyme. Both chromatographic methods revealed a single peak corresponding to PG activity. PG enzyme activity from healthy and wounded pear tissue was undetectable, which supports the claim that the purified PG is of fungal origin. The purified enzyme had a molecular mass of 41 kDa and a pI of 7.8. Activity of the PG was not associated with a glycosylated protein. The enzyme was active over a broad pH range from 3 to 6, with optimal activity at 4.5 in sodium citrate and sodium acetate buffers. The optimal temperature for activity was 37 degrees C but the enzyme was also active at 0, 5, 10, 20, and 50 degrees C. Thin-layer chromatographic analysis of PG hydrolysis products showed that the enzyme exhibits endo- and exo-activity. The purified enzyme macerated tissue in vitro causing approximate to 30% reduction in mass of pear plugs compared with approximate to 17% reduction for apple. Additionally, it produced 1.5-fold more soluble polyuronides on pear than apple tissue. This work shows for the first time the production of a PG by P. expansum during postharvest decay of pear fruit is different from the previously described PG produced in decayed apple fruit by the same pathogen.en
dc.publisherAmer Phytopathological Soc, St Paul
dc.relationUSDAUnited States Department of Agriculture (USDA) [1275-42430-008-00D]
dc.rightsopenAccess
dc.sourcePhytopathology
dc.subjecthost specificityen
dc.subjectmacerationen
dc.titlePurification and Biochemical Characterization of Polygalacturonase Produced by Penicillium expansum During Postharvest Decay of 'Anjou' Pearen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage48
dc.citation.issue1
dc.citation.other100(1): 42-48
dc.citation.rankM21
dc.citation.spage42
dc.citation.volume100
dc.identifier.doi10.1094/PHYTO-100-1-0042
dc.identifier.fulltexthttp://aspace.agrif.bg.ac.rs/bitstream/id/1171/2372.pdf
dc.identifier.scopus2-s2.0-75649087353
dc.identifier.pmid19968548
dc.identifier.wos000272496500004
dc.type.versionpublishedVersion


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Приказ основних података о документу