Examining self-compatibility in plum (Prunus domestica L.) by fluorescence microscopy

Abstract

Self-compatibility in 18 European plum cultivars was examined using the method of fluorescence microscopy. According to selfcompatibility, cultivars were divided into two groups: self-compatible and self-incompatible. In self-compatible cultivars the number of pistils, where pollen tubes reached the base of the style varied from 32.00% (Anna Späth) to 91.18% (Wangenheims Frühzwetsche). Mean number of pollen tubes at the base of style in these cultivars ranged from 0.52 to 3.97. Cultivars were considered self-incompatible if pollen tubes stopped their growth in the style along with forming characteristic swellings at their tips. Of the studied cultivars, 13 were found to be self-compatible: Wangenheims Frühzwetsche, Cacanska Lepotica, Valjevka, California Blue, Cacanska Rodna, Italian Prune, Stanley, Požegaca, Herman, Bluefre, Jelica, Ruth Gerstetter and Anna Späth, while 5 were found to be self-incompatible: Cacanska Rana, Zimmers Frühzwetsche, Cacanska Najbolja, Pacific and President.

Kod 18 sorti domaće šljive ispitivana je samooplodnost metodom fluorescentne mikroskopije. Na osnovu samooplodnosti sorte su podeljene u dve grupe: autokompatibilne i autoinkompatibilne. Kod autokompatibilnih sorti broj tučkova kod kojih su polenove cevčice stigle do osnove stubića varirao je od 32,00% (Ana Špet) do 91,18% (Vangenhajmova). Prosečan broj polenovih cevčica u osnovi stubića kod ovih sorti je bio od 0,52 do 3,97. Sorte su smatrane autoinkompatibilnim ako su polenove cevčice zaustavljale rast u stubiću tucka uz formiranje karakterističnih zadebljanja na njihovim krajevima. Među proučavanim sortama 13 su se pokazale kao autokompatibilne: Vangenhajmova, Čačanska lepotica, Valjevka, Kalifornijska plava, Čačanska rodna, Italijanka, Stenli, Požegača, Herman, Blufri, Jelica, Rut geršteter i Ana Špet, a 5 kao autoinkompatibilne: Čačanska rana, Cimerova rana, ČaČanska najbolja, Pacifik i Prezident.