Establishing the resistance of Myzus persicae (Sulzer) by molecular methods
Utvrđivanje rezistentnosti Myzus persicae (Sulzer) molekularnim metodama
Abstract
In two years of investigating resistance of the peach-potato aphid Myzus persicae (Sulzer) by molecular methods, several types of resistance were established in the majority of individuals from peach and tobacco in Serbia and Montenegro. Most of the tested individuals had the FE4 gene, which encodes production of FE4 esterase. The gene responsible for kdr (knock-down resistance) was found in the majority of individuals, but in the heterozygous state, while resistance based on formation of modified acetlycholinesterase (MACE) was least represented. Also, tests showed aphids from tobacco to be more sensitive to insecticide action than aphids from peach. Three tests were used in these investigations, e.g., the PCR - esterase, PCR - kdr, and RFLP - PCR tests, each for a single type of resistance.
U dvogodišnjim istraživanjima rezistentnosti zelene breskvine vaši, Myzus persicae (Sulzer) molekularnim metodama, ustanovljeno je da je kod većine testiranih jedinki sa breskve i duvana u Srbiji i Crnoj Gori, utvrđen neki od tipova rezistentnosti. Većina testiranih jedinki imala je FE4 gen koji kodira produkciju FE4 karboksilesteraze. Gen odgovoran za kdr (knock-down rezistentnost) nađen je kod većine jedinki, ali u heterozigotnom stanju dok je rezistentnost koja se zasniva na stvaranju modifikovane acetilholinesteraze (MACE) najmanje zastupljena. Takođe, testovi su pokazali da su vaši sa duvana osetljivije na dejstvo insekticida od vašiju sa breskve. U ovim istraživanjima korišćena su tri testa: PCR-esteraza test, PCR-kdr i RFLP-PCR, svaki za po jedan tip rezistentnosti.
Keywords:
Myzus persicae persicae / M. persicae nicotianae / resistance / esterase / kdr / MACE / Serbia / MontenegroSource:
Archives of Biological Sciences, 2008, 60, 3, 493-499Publisher:
- Srpsko biološko društvo, Beograd, i dr.
Funding / projects:
DOI: 10.2298/ABS0803493V
ISSN: 0354-4664
WoS: 000258524700018
Scopus: 2-s2.0-53549093031
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Institution/Community
Poljoprivredni fakultetTY - JOUR AU - Vučetić, Andja AU - Petrović-Obradović, Olivera AU - Margaritopoulos, J. AU - Skouras, P. PY - 2008 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/1831 AB - In two years of investigating resistance of the peach-potato aphid Myzus persicae (Sulzer) by molecular methods, several types of resistance were established in the majority of individuals from peach and tobacco in Serbia and Montenegro. Most of the tested individuals had the FE4 gene, which encodes production of FE4 esterase. The gene responsible for kdr (knock-down resistance) was found in the majority of individuals, but in the heterozygous state, while resistance based on formation of modified acetlycholinesterase (MACE) was least represented. Also, tests showed aphids from tobacco to be more sensitive to insecticide action than aphids from peach. Three tests were used in these investigations, e.g., the PCR - esterase, PCR - kdr, and RFLP - PCR tests, each for a single type of resistance. AB - U dvogodišnjim istraživanjima rezistentnosti zelene breskvine vaši, Myzus persicae (Sulzer) molekularnim metodama, ustanovljeno je da je kod većine testiranih jedinki sa breskve i duvana u Srbiji i Crnoj Gori, utvrđen neki od tipova rezistentnosti. Većina testiranih jedinki imala je FE4 gen koji kodira produkciju FE4 karboksilesteraze. Gen odgovoran za kdr (knock-down rezistentnost) nađen je kod većine jedinki, ali u heterozigotnom stanju dok je rezistentnost koja se zasniva na stvaranju modifikovane acetilholinesteraze (MACE) najmanje zastupljena. Takođe, testovi su pokazali da su vaši sa duvana osetljivije na dejstvo insekticida od vašiju sa breskve. U ovim istraživanjima korišćena su tri testa: PCR-esteraza test, PCR-kdr i RFLP-PCR, svaki za po jedan tip rezistentnosti. PB - Srpsko biološko društvo, Beograd, i dr. T2 - Archives of Biological Sciences T1 - Establishing the resistance of Myzus persicae (Sulzer) by molecular methods T1 - Utvrđivanje rezistentnosti Myzus persicae (Sulzer) molekularnim metodama EP - 499 IS - 3 SP - 493 VL - 60 DO - 10.2298/ABS0803493V ER -
@article{ author = "Vučetić, Andja and Petrović-Obradović, Olivera and Margaritopoulos, J. and Skouras, P.", year = "2008", abstract = "In two years of investigating resistance of the peach-potato aphid Myzus persicae (Sulzer) by molecular methods, several types of resistance were established in the majority of individuals from peach and tobacco in Serbia and Montenegro. Most of the tested individuals had the FE4 gene, which encodes production of FE4 esterase. The gene responsible for kdr (knock-down resistance) was found in the majority of individuals, but in the heterozygous state, while resistance based on formation of modified acetlycholinesterase (MACE) was least represented. Also, tests showed aphids from tobacco to be more sensitive to insecticide action than aphids from peach. Three tests were used in these investigations, e.g., the PCR - esterase, PCR - kdr, and RFLP - PCR tests, each for a single type of resistance., U dvogodišnjim istraživanjima rezistentnosti zelene breskvine vaši, Myzus persicae (Sulzer) molekularnim metodama, ustanovljeno je da je kod većine testiranih jedinki sa breskve i duvana u Srbiji i Crnoj Gori, utvrđen neki od tipova rezistentnosti. Većina testiranih jedinki imala je FE4 gen koji kodira produkciju FE4 karboksilesteraze. Gen odgovoran za kdr (knock-down rezistentnost) nađen je kod većine jedinki, ali u heterozigotnom stanju dok je rezistentnost koja se zasniva na stvaranju modifikovane acetilholinesteraze (MACE) najmanje zastupljena. Takođe, testovi su pokazali da su vaši sa duvana osetljivije na dejstvo insekticida od vašiju sa breskve. U ovim istraživanjima korišćena su tri testa: PCR-esteraza test, PCR-kdr i RFLP-PCR, svaki za po jedan tip rezistentnosti.", publisher = "Srpsko biološko društvo, Beograd, i dr.", journal = "Archives of Biological Sciences", title = "Establishing the resistance of Myzus persicae (Sulzer) by molecular methods, Utvrđivanje rezistentnosti Myzus persicae (Sulzer) molekularnim metodama", pages = "499-493", number = "3", volume = "60", doi = "10.2298/ABS0803493V" }
Vučetić, A., Petrović-Obradović, O., Margaritopoulos, J.,& Skouras, P.. (2008). Establishing the resistance of Myzus persicae (Sulzer) by molecular methods. in Archives of Biological Sciences Srpsko biološko društvo, Beograd, i dr.., 60(3), 493-499. https://doi.org/10.2298/ABS0803493V
Vučetić A, Petrović-Obradović O, Margaritopoulos J, Skouras P. Establishing the resistance of Myzus persicae (Sulzer) by molecular methods. in Archives of Biological Sciences. 2008;60(3):493-499. doi:10.2298/ABS0803493V .
Vučetić, Andja, Petrović-Obradović, Olivera, Margaritopoulos, J., Skouras, P., "Establishing the resistance of Myzus persicae (Sulzer) by molecular methods" in Archives of Biological Sciences, 60, no. 3 (2008):493-499, https://doi.org/10.2298/ABS0803493V . .
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