Studies on genetic control of low-molecular-weight glutenin subunits in wheat endosperm by A-PAGE analysis
Апстракт
Genetic control of low-molecular-weight glutenin subunits (LMW-GS) of nullisomictetrasomic (NT) and ditelocentric (DT) lines of Chinese Spring, and substitution lines of Cheyenne-Wichiita were investigated using acid-polyacrylamide gel electrophoresis (A-PAGE) analysis. The results showed that genes coding for about 20 LMW-GS could be localized on the short arms (Glu-3 loci) of homologous group 1 chromosomes. On the basis of linkages between Gli-1 loci and Glu-3 loci, several LMW-GS were showed to be coded by different Glu-3 loci by the analysis of F3 generation of Sana x Francuska hybrid. Of 8 LMW glutenin subunits analyzed, 2, 3 and 3 LMW-GS were controlled by Glu-A3, Glu-B3 and Glu-D3 locus, respectively. A-PAGE was capable of rapid, high-resolution separation of glutenin subunits. This system, thus, may become a more efficient alternative to routine SDS-PAGE for investigating composition and genetic control of wheat glutenin subunits.
Кључне речи:
A-PAGE / Genetic control / LMW-GS / WheatИзвор:
Cereal Research Communications, 1999, 27, 3, 251-257Институција/група
Poljoprivredni fakultetTY - JOUR AU - Yan, Y. AU - Prodanović, Slaven AU - Mladenov, N. AU - Milovanović, M. PY - 1999 UR - http://aspace.agrif.bg.ac.rs/handle/123456789/147 AB - Genetic control of low-molecular-weight glutenin subunits (LMW-GS) of nullisomictetrasomic (NT) and ditelocentric (DT) lines of Chinese Spring, and substitution lines of Cheyenne-Wichiita were investigated using acid-polyacrylamide gel electrophoresis (A-PAGE) analysis. The results showed that genes coding for about 20 LMW-GS could be localized on the short arms (Glu-3 loci) of homologous group 1 chromosomes. On the basis of linkages between Gli-1 loci and Glu-3 loci, several LMW-GS were showed to be coded by different Glu-3 loci by the analysis of F3 generation of Sana x Francuska hybrid. Of 8 LMW glutenin subunits analyzed, 2, 3 and 3 LMW-GS were controlled by Glu-A3, Glu-B3 and Glu-D3 locus, respectively. A-PAGE was capable of rapid, high-resolution separation of glutenin subunits. This system, thus, may become a more efficient alternative to routine SDS-PAGE for investigating composition and genetic control of wheat glutenin subunits. T2 - Cereal Research Communications T1 - Studies on genetic control of low-molecular-weight glutenin subunits in wheat endosperm by A-PAGE analysis EP - 257 IS - 3 SP - 251 VL - 27 DO - 10.1007/bf03543533 ER -
@article{ author = "Yan, Y. and Prodanović, Slaven and Mladenov, N. and Milovanović, M.", year = "1999", abstract = "Genetic control of low-molecular-weight glutenin subunits (LMW-GS) of nullisomictetrasomic (NT) and ditelocentric (DT) lines of Chinese Spring, and substitution lines of Cheyenne-Wichiita were investigated using acid-polyacrylamide gel electrophoresis (A-PAGE) analysis. The results showed that genes coding for about 20 LMW-GS could be localized on the short arms (Glu-3 loci) of homologous group 1 chromosomes. On the basis of linkages between Gli-1 loci and Glu-3 loci, several LMW-GS were showed to be coded by different Glu-3 loci by the analysis of F3 generation of Sana x Francuska hybrid. Of 8 LMW glutenin subunits analyzed, 2, 3 and 3 LMW-GS were controlled by Glu-A3, Glu-B3 and Glu-D3 locus, respectively. A-PAGE was capable of rapid, high-resolution separation of glutenin subunits. This system, thus, may become a more efficient alternative to routine SDS-PAGE for investigating composition and genetic control of wheat glutenin subunits.", journal = "Cereal Research Communications", title = "Studies on genetic control of low-molecular-weight glutenin subunits in wheat endosperm by A-PAGE analysis", pages = "257-251", number = "3", volume = "27", doi = "10.1007/bf03543533" }
Yan, Y., Prodanović, S., Mladenov, N.,& Milovanović, M.. (1999). Studies on genetic control of low-molecular-weight glutenin subunits in wheat endosperm by A-PAGE analysis. in Cereal Research Communications, 27(3), 251-257. https://doi.org/10.1007/bf03543533
Yan Y, Prodanović S, Mladenov N, Milovanović M. Studies on genetic control of low-molecular-weight glutenin subunits in wheat endosperm by A-PAGE analysis. in Cereal Research Communications. 1999;27(3):251-257. doi:10.1007/bf03543533 .
Yan, Y., Prodanović, Slaven, Mladenov, N., Milovanović, M., "Studies on genetic control of low-molecular-weight glutenin subunits in wheat endosperm by A-PAGE analysis" in Cereal Research Communications, 27, no. 3 (1999):251-257, https://doi.org/10.1007/bf03543533 . .