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Investigations of cell immobilization in alginate: rheological and electrostatic extrusion studies

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2006
1257.pdf (134.7Kb)
Authors
Manojlović, Verica
Djonlagić, Jasna
Obradović, B
Nedović, Viktor
Bugarski, Branko
Conference object (Published version)
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Abstract
In this study, the process of electrostatic extrusion as a method for cell immobilization was investigated. We have assessed the effects of concentrations of yeast cells (as a model cell type) and Na alginate on the size of the resulting microbeads and attempted to rationalize the obtained findings by rheological characterization of the cell-alginate suspensions. Under the investigated conditions, microbeads, 50-600 mu m in diameter, were produced and the increase in both alginate and cell concentrations resulted in larger microbeads with their sizes having higher standard deviations. Rheological characterization revealed non-Newtonian, pseudoplastic behavior of cell-alginate suspensions with higher viscosities at higher alginate concentrations. However, the presence of cells even at high concentrations (5 x 10(8) and 1 x 10(9) cells mL(-1)) did not significantly affect the rheological properties of the Na alginate solution. Finally, we have investigated the kinetics of alginate gelati...on with respect to the quantity of Ca2+ ions and the presence of cells. The molar ratio of alpha-L-guluronic acid units to Ca2+ ions of 4:1 provided complete crosslinking. The presence of cells decreased the rate of network formation as well as the strength of the obtained Ca alginate hydrogel.

Keywords:
cell immobilization / rheology / alginate / electrostatic extrusion
Source:
Journal of Chemical Technology and Biotechnology, 2006, 81, 4, 505-510
Publisher:
  • Wiley, Hoboken

DOI: 10.1002/jctb.1465

ISSN: 0268-2575

WoS: 000236946500005

Scopus: 2-s2.0-33646205685
[ Google Scholar ]
22
24
URI
http://aspace.agrif.bg.ac.rs/handle/123456789/1260
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Poljoprivredni fakultet
TY  - CONF
AU  - Manojlović, Verica
AU  - Djonlagić, Jasna
AU  - Obradović, B
AU  - Nedović, Viktor
AU  - Bugarski, Branko
PY  - 2006
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1260
AB  - In this study, the process of electrostatic extrusion as a method for cell immobilization was investigated. We have assessed the effects of concentrations of yeast cells (as a model cell type) and Na alginate on the size of the resulting microbeads and attempted to rationalize the obtained findings by rheological characterization of the cell-alginate suspensions. Under the investigated conditions, microbeads, 50-600 mu m in diameter, were produced and the increase in both alginate and cell concentrations resulted in larger microbeads with their sizes having higher standard deviations. Rheological characterization revealed non-Newtonian, pseudoplastic behavior of cell-alginate suspensions with higher viscosities at higher alginate concentrations. However, the presence of cells even at high concentrations (5 x 10(8) and 1 x 10(9) cells mL(-1)) did not significantly affect the rheological properties of the Na alginate solution. Finally, we have investigated the kinetics of alginate gelation with respect to the quantity of Ca2+ ions and the presence of cells. The molar ratio of alpha-L-guluronic acid units to Ca2+ ions of 4:1 provided complete crosslinking. The presence of cells decreased the rate of network formation as well as the strength of the obtained Ca alginate hydrogel.
PB  - Wiley, Hoboken
C3  - Journal of Chemical Technology and Biotechnology
T1  - Investigations of cell immobilization in alginate: rheological and electrostatic extrusion studies
EP  - 510
IS  - 4
SP  - 505
VL  - 81
DO  - 10.1002/jctb.1465
UR  - conv_4017
ER  - 
@conference{
author = "Manojlović, Verica and Djonlagić, Jasna and Obradović, B and Nedović, Viktor and Bugarski, Branko",
year = "2006",
abstract = "In this study, the process of electrostatic extrusion as a method for cell immobilization was investigated. We have assessed the effects of concentrations of yeast cells (as a model cell type) and Na alginate on the size of the resulting microbeads and attempted to rationalize the obtained findings by rheological characterization of the cell-alginate suspensions. Under the investigated conditions, microbeads, 50-600 mu m in diameter, were produced and the increase in both alginate and cell concentrations resulted in larger microbeads with their sizes having higher standard deviations. Rheological characterization revealed non-Newtonian, pseudoplastic behavior of cell-alginate suspensions with higher viscosities at higher alginate concentrations. However, the presence of cells even at high concentrations (5 x 10(8) and 1 x 10(9) cells mL(-1)) did not significantly affect the rheological properties of the Na alginate solution. Finally, we have investigated the kinetics of alginate gelation with respect to the quantity of Ca2+ ions and the presence of cells. The molar ratio of alpha-L-guluronic acid units to Ca2+ ions of 4:1 provided complete crosslinking. The presence of cells decreased the rate of network formation as well as the strength of the obtained Ca alginate hydrogel.",
publisher = "Wiley, Hoboken",
journal = "Journal of Chemical Technology and Biotechnology",
title = "Investigations of cell immobilization in alginate: rheological and electrostatic extrusion studies",
pages = "510-505",
number = "4",
volume = "81",
doi = "10.1002/jctb.1465",
url = "conv_4017"
}
Manojlović, V., Djonlagić, J., Obradović, B., Nedović, V.,& Bugarski, B.. (2006). Investigations of cell immobilization in alginate: rheological and electrostatic extrusion studies. in Journal of Chemical Technology and Biotechnology
Wiley, Hoboken., 81(4), 505-510.
https://doi.org/10.1002/jctb.1465
conv_4017
Manojlović V, Djonlagić J, Obradović B, Nedović V, Bugarski B. Investigations of cell immobilization in alginate: rheological and electrostatic extrusion studies. in Journal of Chemical Technology and Biotechnology. 2006;81(4):505-510.
doi:10.1002/jctb.1465
conv_4017 .
Manojlović, Verica, Djonlagić, Jasna, Obradović, B, Nedović, Viktor, Bugarski, Branko, "Investigations of cell immobilization in alginate: rheological and electrostatic extrusion studies" in Journal of Chemical Technology and Biotechnology, 81, no. 4 (2006):505-510,
https://doi.org/10.1002/jctb.1465 .,
conv_4017 .

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