Coliform bacteria in waters of experimental field 'Radmilovac'

Abstract

Potential risk for human infection are vegetables and fruits, which are contaminated with pathogenic bacteria. Potentional source this contamination are waters for irrigation, underground waters, surface waters or waste disposal waters. Experimental results all over the world confirm concept that each water is not good for irrigation, and it is need to do microbiological analyses of water which is used for irrigation. The main goal of this investigation is determination quality waters in O.Š.D. "Radmilovac" which are used for irrigation. Samples were taken in waters in O.Š.D. "Radmilovac" at two uncovered wells, covered well, stream "Šugavac" (up-stream and down­stream) and public drink-fountain. Samples were taken during the Martch and April. It were determinate total number bacteria, number of total coliform and fecal coliform bacteria, presence of Salmonella sp,, Aeromonas sp. and Listeria sp. Total number bacteria was determinated on Nutrient agar, number of total and fecal coliform on MacConkey broth with method most probably number (MPN), Salmonella sp. was determinated on SS Agar, E. coli on EMB Agaru, Aeromonas sp. on Aeromonas Selective Agar, Listeria sp. on Palcam Agar. Isolated pathogenic bacteria were idetified by API 20E (Biomerieux, France). Results of investigations show that total number bacteria was 10 x 102 - 8.8 x 104 in 1ml. Total coliform bacteria were established in all samples, and fecal coliform bacteria were not found out only in uncovered well 1 and public drink-fountain. Salmonella sp. was established in Šugavac and uncovered wells, E.coli in Šugavac and covered well, Aeromonas sp. was in presence in all samples and Listeria sp. was not established.

Potencijalni rizik za humanu infekciju predstavljaju povrće i voće, kontaminirano patogenim bakterijama. Mogući izvor njihove kontaminacije, su vode za navodnjavanje, podzemne, površinske ili otpadne vode. Ekperimentalni rezultati u svetu potvrđuju koncept da svaka voda nije dobra za navodnjavanje i da je neophodna mikrobiološka analiza vode koja se koristi u ovu svrhu. Cilj ovog rada je ispitivanje kvaliteta vode na O.Š.D. "Radmilovac" koje se koriste za navodnjavanje. Uzorci su uzeti u vodama na O.Š.D. "Radmilovac" i to iz dva otvorena bunara, jednog zatvorenog bunara, potoka "Šugavac" (ulaz i izlaz) i javne česme. Uzorci su uzeti tokom aprila i maja meseca. Određen je ukupan broj bakterija, prisustvo ukupnih i fekalnih koliformnih bakterija, Salmonella sp., Aeromonas sp. i Listeria sp. Ukupan broj je određen na hranljivom agaru, ukupne i fekalne koloformne na MacConkey bujonu metodom verovatnog broja (MPN). Salmonella sp. je određena na SS agaru, E. coli na EMB agaru, Aeromonas sp. na Aeromonas selektivnom agaru, Listeria sp. na Palcam agaru. Izolovane patogene bakterije su identifikovane pomoći API 20E (Biomerieux, France). Rezultati istraživanja su pokazali da se ukupan broj bakterija kretao od 10 x 102 do 8.8 x 104 u l ml. Ukupne koliformne bakterija su konstatovane u svim uzorcima, a fekalne koliformne nisu konstatovane samo u otvorenom bunaru l i javnoj česmi.