TY  - JOUR
AU  - Stanković, Ivana
AU  - Zečević, Katarina
AU  - Delibašić, Goran
AU  - Jović, Jelena
AU  - Toševski, Ivo
AU  - Krstić, Branka
PY  - 2022
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6155
AB  - Grapevine rupestris stem pitting-associated virus (GRSPaV), a member of the genus Foveavirus, is a commonly detected
grapevine virus around the world. Despite the broad and global distribution of GRSPaV, no information on its presence in
Serbia had been available before this study. In October 2018, 3-year-old grapevine plants of ‘Cabernet Sauvignon’ showing
reduced growth and leaf reddening were observed in the vicinity of Krnjevo locality. The results of an mRT-PCR test showed
that only samples prepared from cortical scrapings of mature symptomatic grapevine canes yielded an amplicon of 155 bp
that confrmed the presence of GRSPaV. No amplifcation products were recorded in samples prepared from basal leaves
or petioles. A serological assay confrmed the presence of GRSPaV in all tested mature cane samples. For further analyses,
the CP (coat protein) genes of two selected isolates (VL8 and VL15) of naturally infected grapevine canes were partially
sequenced and six sequence variants were determined, showing a certain variability among them. The constructed phylogenetic tree revealed the grouping of Serbian sequence variants into separate subgroups within the same phylogenetic lineage.
This is the frst report of GRSPaV present in Serbia. Further monitoring and in
PB  - Springer
T2  - Journal of Plant Diseases and Protection
T1  - Grapevine rupestris stem pitting virus: a new pathogen of grapevine in Serbia
IS  - 1
VL  - 1
DO  - 10.1007/s41348-022-00656-x
ER  - 

@article{
author = "Stanković, Ivana and Zečević, Katarina and Delibašić, Goran and Jović, Jelena and Toševski, Ivo and Krstić, Branka",
year = "2022",
abstract = "Grapevine rupestris stem pitting-associated virus (GRSPaV), a member of the genus Foveavirus, is a commonly detected
grapevine virus around the world. Despite the broad and global distribution of GRSPaV, no information on its presence in
Serbia had been available before this study. In October 2018, 3-year-old grapevine plants of ‘Cabernet Sauvignon’ showing
reduced growth and leaf reddening were observed in the vicinity of Krnjevo locality. The results of an mRT-PCR test showed
that only samples prepared from cortical scrapings of mature symptomatic grapevine canes yielded an amplicon of 155 bp
that confrmed the presence of GRSPaV. No amplifcation products were recorded in samples prepared from basal leaves
or petioles. A serological assay confrmed the presence of GRSPaV in all tested mature cane samples. For further analyses,
the CP (coat protein) genes of two selected isolates (VL8 and VL15) of naturally infected grapevine canes were partially
sequenced and six sequence variants were determined, showing a certain variability among them. The constructed phylogenetic tree revealed the grouping of Serbian sequence variants into separate subgroups within the same phylogenetic lineage.
This is the frst report of GRSPaV present in Serbia. Further monitoring and in",
publisher = "Springer",
journal = "Journal of Plant Diseases and Protection",
title = "Grapevine rupestris stem pitting virus: a new pathogen of grapevine in Serbia",
number = "1",
volume = "1",
doi = "10.1007/s41348-022-00656-x"
}

Stanković, I., Zečević, K., Delibašić, G., Jović, J., Toševski, I.,& Krstić, B.. (2022). Grapevine rupestris stem pitting virus: a new pathogen of grapevine in Serbia. in Journal of Plant Diseases and Protection
Springer., 1(1).
https://doi.org/10.1007/s41348-022-00656-x

Stanković I, Zečević K, Delibašić G, Jović J, Toševski I, Krstić B. Grapevine rupestris stem pitting virus: a new pathogen of grapevine in Serbia. in Journal of Plant Diseases and Protection. 2022;1(1).
doi:10.1007/s41348-022-00656-x .

Stanković, Ivana, Zečević, Katarina, Delibašić, Goran, Jović, Jelena, Toševski, Ivo, Krstić, Branka, "Grapevine rupestris stem pitting virus: a new pathogen of grapevine in Serbia" in Journal of Plant Diseases and Protection, 1, no. 1 (2022),
https://doi.org/10.1007/s41348-022-00656-x . .

TY  - CONF
AU  - Toševski, Ivo
AU  - Krstić, Oliver
AU  - Jović, Jelena
AU  - Vidović, Biljana
AU  - Petanović, Radmila
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4686
AB  - Territory of the Republic of Serbia, as the central area of the Balkan Peninsula, is of particular interest as a point where biological entities coming from central Europe, Asia Minor, Mediterranean and North Africa encounter. Hence, this area has been declared as a "center of European biodiversity". Natural consequence of Serbia's geographic position and the richness of the flora is the great diversity of insect fauna (Insecta). Observed diversity of entomofauna is the reason why the territory of Serbia is attractive for the research in biological control. Climate change and the intensive exchange of biota as a direct consequence of increasing global trend of trade between the countries is what make this a particularly important issue. Although Serbia has a relatively short history of research on phytophagous insects as biological control agents for Eurasian origin weeds, researchers at the biological laboratory of Institute for plant protection based in Zemun (presently Institute for plant protection and environment, Zemun) can be considered as doyens of biological control research in Europe and even worldwide. The first department for biological control of pests in Europe started working in the Institute for plant protection in Zemun, in 1954. The founder and the first director of the Institute for plant protection, prof. dr. Guido Nonveiller, had a vision of forming a team of interdisciplinary researchers with biological and agronomic BACKGROUND: . In the period from 1967-1974, the project entitled "Project 480: Europe: Yugoslavia E30-Ent-12 - Distribution and natural enemies of the weeds Dalmatian toadflax (Linaria dalmatica Mill) and Mediterranean sage (Salvia aethiopsis L.) in Yugoslavia", has been carried out for the purpose of biological control of L. dalmatica (Plantaginaceae) and Salvia aethiops (Lamiaceae). Four more projects supported by the USDA followed: Elaboration of protective measures for agricultural crops against parasitic phanerogams belonging to the genera Cuscuta and Orobanche (parasitic herbs - dodders and broomrape) (1968-1971), Biological control of terrestrial and aquatic weeds and pests of crop plants (1977-1983), Biological control of insects and weeds (1981-1986), Biocontrol of Yugoslavian and North American weeds with insects, pathogens and other biotic agents (1988-1990, 1991). Research on potential phytophagous insects against invasive weeds in North America included, beside a forementioned plants, Myriophyllum spicatum L. (Haloragaceae), Carduus nutans, Carduus acanthoides, Cirsium arvense, Centaurea solstitialis, Centaurea maculosa (Asteraceae), Euphorbia cyparissias and Euphorbia virgata (Euphorbaceae). Classical biological control (i.e. intentional release of natural enemies of exotic pests for the purpose of their control) represents a very important tool in the integrated pest management. Globalization and intensive trade between countries have led to exchange of organisms from different zoogeographical regions. Should those organisms adapt to the new environment, they could potentially represent threat to local ecosystems. This is the reason why, according to Agenda 21 (Chapter 14), biological control has been recognized as a method of choice in pest control. Until 1992, the research within USDA Project 480 resulted in introduction of several insect species in the area of North America. Introduced species were: Sphenoptera jugoslavica for the purpose of controlling Centaurea diffusa (Asteraceae), Hylas euphorbiae, Oberea erythrocephala, Pegomya euphorbiae and P. curticornis (Diptera: Anthomyiidae) for the control of invasive spurge species Euphorbia esula, E. cyparissias and E. virgata. Species from the genus Aphthona (Coleoptera, Chrysomelidae), A. lacertosa, A. nigris-cutis and A. flava, were introduced for the same reason. Further, two weevil species, Larinus obtusus and L. minutus (Coleoptera, Curculionidae), were used against Centaurea diffusa and Centaurea maculosa. Lastly, defoliator species Calophasia lunula (Lepidoptera: Noctuidae) was introduced for the control of Linaria dalmatica and L. vulgaris (Plantaginaceae). During the period of 1992-1999, introduced species were Chamaesphecia hungarica and Ch. crassicornis for the control of Euphorbia virgata and Chamaesphecia hungarica for the control of Euphorbia esula. Two moth species, Eteobalea serratella and E. intermediella (Cosmopterigidae), were introduced for the control of north American populations of Linaria vulgaris and L. dalmatica. Finally, after extensive host specificity tests, a weevil species Mogulones cruciger was introduced for the purpose of controlling Cynoglossum officinale (Boraginaceae) in North America. Advances in molecular techniques in the 21st century have led to turn over in biological control research. New knowledge on specific interactions within the insect-plant system has placed in the focus population-genetic properties of the biological control agent. Molecular techniques also enabled understanding biological properties of local insect's populations as well as cryptic speciation, which consequently led to overall better safety and precision in biological control research. The research, since 2000, has been focused on invasive plant species of the genus Linaria and weevils of the genus Mecinus and Rhinusa. New research enabled introduction and successful development of gallicol weevil species Rhinusapilosa in north-west Canada. Furthermore, cryptic species Mecinus janthiniformis was described within the Mecinus jathinus. Within Mecinus heydenii complex of species, one cryptic species were described, Mecinus peterharrisi in association with L. dalmatica and one subspecies Mecinus laeviceps ssp. meridionalis association with L. genistifolia from East Serbia and Bulgaria. One new gallforming weevil species was also described, namely Rhinusa rara in association with L. genistifolia and L. dalmatica from East Serbia, Hungary and South Russia. All newly described species and subspecies are in the research process of suitability assessment as the biological control agents. Besides insects many species of eriophyoid mites, an obligatory plant feeders, are considered to be among the most promising candidate agents for classical biological control of weeds due to their biology and host-plant relationship. Eriophyoid mites have high degree of host specificity, about 80% of currently known species have been recorded on a one host plant. Among approximately 5000 known species about 50 are considered as potential weed control agents in the world. More than 50% of these species have been discovered in Serbia. Investigations in this field started in early 1980's. In the period 1990-2000's in the framework of different projects three new species for science were described on Geranium dissectum L. and four on Euphorbia spp. At the beginning of 2000's, especially detailed studies of Aceria anthocoptes (Nal.) and Leipothrix dipsacivagus Petanovic. et Rector have been carried out from the different points of view. During the last years few eriophyoid mites were investigated within the framework of different European programs of classical biological control of weeds. Aceria acroptiloni Kovalev et Shevchenko was studied as agent of biocontrol of Rhaponticum repens (L.) Hidalgo, Metaculus spp. were studied for biological control of Lepidium latifolium L., Isatis tinctoria and Diplotaxis tenuifolia, as well as two Aceria spp. for biological control of Eleagnus angustifolia L. Moreover two potential biocontrol agents Aceria artemisiifoliae Vidovic & Petanovic, and Aculops mosiniensis Ripka against Ambrosia artemisifolia L. and Ailanthus altissima (Mill) Swingle respectively have been nominated recently. More or less integrative approach in species delimitation has been applied using combined techniques of phase contrast light microscopy, pseudo dark field, differential interference contrast microscopy, confocal laser scanning microscopy, scanning electron microscopy and sequencing of barcoding region of mt COI DNA. Traditional morphological analysis was accomplished with morphometric, (linear and/or geometric) analysis because it helps in understanding intraspecific variability, including races adapted to the host plants and /or cryptic species. Studying potential agents for the biological control of invasive weeds is tightly linked with floristic and entomo-acaro-faunistic diversity. Because of its position, the territory of Serbia represents a meeting point for floristic and faunistic elements coming from central Europe, Asia Minor, Mediterranean and North Africa. The complex floristic and faunistic diversity is also a consequence of numerous colonizations and recolonizations of biota during the periods of glaciation and interglaciation. These unique aspects have made the territory of Serbia an excellent starting point for the research in biological control.
PB  - Serbian Acad Sciences Arts, Belgrade
C3  - Ecological and Economic Significance of Fauna of Serbia
T1  - Insects and mites in the fauna of Serbia - importance for the classical biological control of weeds
EP  - 365
SP  - 341
VL  - 171
UR  - https://hdl.handle.net/21.15107/rcub_dais_10838
ER  - 

@conference{
author = "Toševski, Ivo and Krstić, Oliver and Jović, Jelena and Vidović, Biljana and Petanović, Radmila",
year = "2018",
abstract = "Territory of the Republic of Serbia, as the central area of the Balkan Peninsula, is of particular interest as a point where biological entities coming from central Europe, Asia Minor, Mediterranean and North Africa encounter. Hence, this area has been declared as a "center of European biodiversity". Natural consequence of Serbia's geographic position and the richness of the flora is the great diversity of insect fauna (Insecta). Observed diversity of entomofauna is the reason why the territory of Serbia is attractive for the research in biological control. Climate change and the intensive exchange of biota as a direct consequence of increasing global trend of trade between the countries is what make this a particularly important issue. Although Serbia has a relatively short history of research on phytophagous insects as biological control agents for Eurasian origin weeds, researchers at the biological laboratory of Institute for plant protection based in Zemun (presently Institute for plant protection and environment, Zemun) can be considered as doyens of biological control research in Europe and even worldwide. The first department for biological control of pests in Europe started working in the Institute for plant protection in Zemun, in 1954. The founder and the first director of the Institute for plant protection, prof. dr. Guido Nonveiller, had a vision of forming a team of interdisciplinary researchers with biological and agronomic BACKGROUND: . In the period from 1967-1974, the project entitled "Project 480: Europe: Yugoslavia E30-Ent-12 - Distribution and natural enemies of the weeds Dalmatian toadflax (Linaria dalmatica Mill) and Mediterranean sage (Salvia aethiopsis L.) in Yugoslavia", has been carried out for the purpose of biological control of L. dalmatica (Plantaginaceae) and Salvia aethiops (Lamiaceae). Four more projects supported by the USDA followed: Elaboration of protective measures for agricultural crops against parasitic phanerogams belonging to the genera Cuscuta and Orobanche (parasitic herbs - dodders and broomrape) (1968-1971), Biological control of terrestrial and aquatic weeds and pests of crop plants (1977-1983), Biological control of insects and weeds (1981-1986), Biocontrol of Yugoslavian and North American weeds with insects, pathogens and other biotic agents (1988-1990, 1991). Research on potential phytophagous insects against invasive weeds in North America included, beside a forementioned plants, Myriophyllum spicatum L. (Haloragaceae), Carduus nutans, Carduus acanthoides, Cirsium arvense, Centaurea solstitialis, Centaurea maculosa (Asteraceae), Euphorbia cyparissias and Euphorbia virgata (Euphorbaceae). Classical biological control (i.e. intentional release of natural enemies of exotic pests for the purpose of their control) represents a very important tool in the integrated pest management. Globalization and intensive trade between countries have led to exchange of organisms from different zoogeographical regions. Should those organisms adapt to the new environment, they could potentially represent threat to local ecosystems. This is the reason why, according to Agenda 21 (Chapter 14), biological control has been recognized as a method of choice in pest control. Until 1992, the research within USDA Project 480 resulted in introduction of several insect species in the area of North America. Introduced species were: Sphenoptera jugoslavica for the purpose of controlling Centaurea diffusa (Asteraceae), Hylas euphorbiae, Oberea erythrocephala, Pegomya euphorbiae and P. curticornis (Diptera: Anthomyiidae) for the control of invasive spurge species Euphorbia esula, E. cyparissias and E. virgata. Species from the genus Aphthona (Coleoptera, Chrysomelidae), A. lacertosa, A. nigris-cutis and A. flava, were introduced for the same reason. Further, two weevil species, Larinus obtusus and L. minutus (Coleoptera, Curculionidae), were used against Centaurea diffusa and Centaurea maculosa. Lastly, defoliator species Calophasia lunula (Lepidoptera: Noctuidae) was introduced for the control of Linaria dalmatica and L. vulgaris (Plantaginaceae). During the period of 1992-1999, introduced species were Chamaesphecia hungarica and Ch. crassicornis for the control of Euphorbia virgata and Chamaesphecia hungarica for the control of Euphorbia esula. Two moth species, Eteobalea serratella and E. intermediella (Cosmopterigidae), were introduced for the control of north American populations of Linaria vulgaris and L. dalmatica. Finally, after extensive host specificity tests, a weevil species Mogulones cruciger was introduced for the purpose of controlling Cynoglossum officinale (Boraginaceae) in North America. Advances in molecular techniques in the 21st century have led to turn over in biological control research. New knowledge on specific interactions within the insect-plant system has placed in the focus population-genetic properties of the biological control agent. Molecular techniques also enabled understanding biological properties of local insect's populations as well as cryptic speciation, which consequently led to overall better safety and precision in biological control research. The research, since 2000, has been focused on invasive plant species of the genus Linaria and weevils of the genus Mecinus and Rhinusa. New research enabled introduction and successful development of gallicol weevil species Rhinusapilosa in north-west Canada. Furthermore, cryptic species Mecinus janthiniformis was described within the Mecinus jathinus. Within Mecinus heydenii complex of species, one cryptic species were described, Mecinus peterharrisi in association with L. dalmatica and one subspecies Mecinus laeviceps ssp. meridionalis association with L. genistifolia from East Serbia and Bulgaria. One new gallforming weevil species was also described, namely Rhinusa rara in association with L. genistifolia and L. dalmatica from East Serbia, Hungary and South Russia. All newly described species and subspecies are in the research process of suitability assessment as the biological control agents. Besides insects many species of eriophyoid mites, an obligatory plant feeders, are considered to be among the most promising candidate agents for classical biological control of weeds due to their biology and host-plant relationship. Eriophyoid mites have high degree of host specificity, about 80% of currently known species have been recorded on a one host plant. Among approximately 5000 known species about 50 are considered as potential weed control agents in the world. More than 50% of these species have been discovered in Serbia. Investigations in this field started in early 1980's. In the period 1990-2000's in the framework of different projects three new species for science were described on Geranium dissectum L. and four on Euphorbia spp. At the beginning of 2000's, especially detailed studies of Aceria anthocoptes (Nal.) and Leipothrix dipsacivagus Petanovic. et Rector have been carried out from the different points of view. During the last years few eriophyoid mites were investigated within the framework of different European programs of classical biological control of weeds. Aceria acroptiloni Kovalev et Shevchenko was studied as agent of biocontrol of Rhaponticum repens (L.) Hidalgo, Metaculus spp. were studied for biological control of Lepidium latifolium L., Isatis tinctoria and Diplotaxis tenuifolia, as well as two Aceria spp. for biological control of Eleagnus angustifolia L. Moreover two potential biocontrol agents Aceria artemisiifoliae Vidovic & Petanovic, and Aculops mosiniensis Ripka against Ambrosia artemisifolia L. and Ailanthus altissima (Mill) Swingle respectively have been nominated recently. More or less integrative approach in species delimitation has been applied using combined techniques of phase contrast light microscopy, pseudo dark field, differential interference contrast microscopy, confocal laser scanning microscopy, scanning electron microscopy and sequencing of barcoding region of mt COI DNA. Traditional morphological analysis was accomplished with morphometric, (linear and/or geometric) analysis because it helps in understanding intraspecific variability, including races adapted to the host plants and /or cryptic species. Studying potential agents for the biological control of invasive weeds is tightly linked with floristic and entomo-acaro-faunistic diversity. Because of its position, the territory of Serbia represents a meeting point for floristic and faunistic elements coming from central Europe, Asia Minor, Mediterranean and North Africa. The complex floristic and faunistic diversity is also a consequence of numerous colonizations and recolonizations of biota during the periods of glaciation and interglaciation. These unique aspects have made the territory of Serbia an excellent starting point for the research in biological control.",
publisher = "Serbian Acad Sciences Arts, Belgrade",
journal = "Ecological and Economic Significance of Fauna of Serbia",
title = "Insects and mites in the fauna of Serbia - importance for the classical biological control of weeds",
pages = "365-341",
volume = "171",
url = "https://hdl.handle.net/21.15107/rcub_dais_10838"
}

Toševski, I., Krstić, O., Jović, J., Vidović, B.,& Petanović, R.. (2018). Insects and mites in the fauna of Serbia - importance for the classical biological control of weeds. in Ecological and Economic Significance of Fauna of Serbia
Serbian Acad Sciences Arts, Belgrade., 171, 341-365.
https://hdl.handle.net/21.15107/rcub_dais_10838

Toševski I, Krstić O, Jović J, Vidović B, Petanović R. Insects and mites in the fauna of Serbia - importance for the classical biological control of weeds. in Ecological and Economic Significance of Fauna of Serbia. 2018;171:341-365.
https://hdl.handle.net/21.15107/rcub_dais_10838 .

Toševski, Ivo, Krstić, Oliver, Jović, Jelena, Vidović, Biljana, Petanović, Radmila, "Insects and mites in the fauna of Serbia - importance for the classical biological control of weeds" in Ecological and Economic Significance of Fauna of Serbia, 171 (2018):341-365,
https://hdl.handle.net/21.15107/rcub_dais_10838 .

TY  - JOUR
AU  - Hamidović, Saud
AU  - Teodorović, Smilja
AU  - Lalević, Blažo
AU  - Jovičić-Petrović, Jelena
AU  - Jović, Jelena
AU  - Kiković, Dragan
AU  - Raičević, Vera
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4103
AB  - Coal and lignite play a major energy supply role in many European countries, including Bosnia and Herzegovina. Yet mining activities are a heavy source of ecosystem contamination, posing significant environmental threats. The primary goal of this study was to isolate and identify autochthonous lignite mine spoil bacteria and evaluate their potential in bioremediation of these polluted soils. Two Bacillus species, Bacillus simplex and a Bacillus cereus group member, were identified using conventional, molecular, and bioinformatics approaches. This represents, to our knowledge, the first microbial characterization of mine overburden in Bosnia and Herzegovina. A co-inoculum of autochthonous bacterial populations was used to treat unvegetated as well as oat- and lettuce-vegetated lignite overburden samples. Our results illustrate the potential of recovered native species to enrich soil fertility and productivity through plant growth promotion.
PB  - Hard, Olsztyn 5
T2  - Polish Journal of Environmental Studies
T1  - Bioremediation Potential Assessment of Plant Growth-Promoting Autochthonous Bacteria: a Lignite Mine Case Study
EP  - 119
IS  - 1
SP  - 113
VL  - 25
DO  - 10.15244/pjoes/59465
ER  - 

@article{
author = "Hamidović, Saud and Teodorović, Smilja and Lalević, Blažo and Jovičić-Petrović, Jelena and Jović, Jelena and Kiković, Dragan and Raičević, Vera",
year = "2016",
abstract = "Coal and lignite play a major energy supply role in many European countries, including Bosnia and Herzegovina. Yet mining activities are a heavy source of ecosystem contamination, posing significant environmental threats. The primary goal of this study was to isolate and identify autochthonous lignite mine spoil bacteria and evaluate their potential in bioremediation of these polluted soils. Two Bacillus species, Bacillus simplex and a Bacillus cereus group member, were identified using conventional, molecular, and bioinformatics approaches. This represents, to our knowledge, the first microbial characterization of mine overburden in Bosnia and Herzegovina. A co-inoculum of autochthonous bacterial populations was used to treat unvegetated as well as oat- and lettuce-vegetated lignite overburden samples. Our results illustrate the potential of recovered native species to enrich soil fertility and productivity through plant growth promotion.",
publisher = "Hard, Olsztyn 5",
journal = "Polish Journal of Environmental Studies",
title = "Bioremediation Potential Assessment of Plant Growth-Promoting Autochthonous Bacteria: a Lignite Mine Case Study",
pages = "119-113",
number = "1",
volume = "25",
doi = "10.15244/pjoes/59465"
}

Hamidović, S., Teodorović, S., Lalević, B., Jovičić-Petrović, J., Jović, J., Kiković, D.,& Raičević, V.. (2016). Bioremediation Potential Assessment of Plant Growth-Promoting Autochthonous Bacteria: a Lignite Mine Case Study. in Polish Journal of Environmental Studies
Hard, Olsztyn 5., 25(1), 113-119.
https://doi.org/10.15244/pjoes/59465

Hamidović S, Teodorović S, Lalević B, Jovičić-Petrović J, Jović J, Kiković D, Raičević V. Bioremediation Potential Assessment of Plant Growth-Promoting Autochthonous Bacteria: a Lignite Mine Case Study. in Polish Journal of Environmental Studies. 2016;25(1):113-119.
doi:10.15244/pjoes/59465 .

Hamidović, Saud, Teodorović, Smilja, Lalević, Blažo, Jovičić-Petrović, Jelena, Jović, Jelena, Kiković, Dragan, Raičević, Vera, "Bioremediation Potential Assessment of Plant Growth-Promoting Autochthonous Bacteria: a Lignite Mine Case Study" in Polish Journal of Environmental Studies, 25, no. 1 (2016):113-119,
https://doi.org/10.15244/pjoes/59465 . .

TY  - JOUR
AU  - Milosavljević, A.
AU  - Pfaf-Dolovac, Erika
AU  - Mitrović, M.
AU  - Jović, Jelena
AU  - Toševski, Ivo
AU  - Duduk, Nataša
AU  - Trkulja, Nenad
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3424
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Cercospora carotae, Causal Agent of Cercospora Leaf Spot of Carrot, in Serbia.
EP  - 1153
IS  - 8
SP  - 1153
VL  - 98
DO  - 10.1094/PDIS-08-13-0858-PDN
ER  - 

@article{
author = "Milosavljević, A. and Pfaf-Dolovac, Erika and Mitrović, M. and Jović, Jelena and Toševski, Ivo and Duduk, Nataša and Trkulja, Nenad",
year = "2014",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Cercospora carotae, Causal Agent of Cercospora Leaf Spot of Carrot, in Serbia.",
pages = "1153-1153",
number = "8",
volume = "98",
doi = "10.1094/PDIS-08-13-0858-PDN"
}

Milosavljević, A., Pfaf-Dolovac, E., Mitrović, M., Jović, J., Toševski, I., Duduk, N.,& Trkulja, N.. (2014). First Report of Cercospora carotae, Causal Agent of Cercospora Leaf Spot of Carrot, in Serbia.. in Plant Disease
Amer Phytopathological Soc, St Paul., 98(8), 1153-1153.
https://doi.org/10.1094/PDIS-08-13-0858-PDN

Milosavljević A, Pfaf-Dolovac E, Mitrović M, Jović J, Toševski I, Duduk N, Trkulja N. First Report of Cercospora carotae, Causal Agent of Cercospora Leaf Spot of Carrot, in Serbia.. in Plant Disease. 2014;98(8):1153-1153.
doi:10.1094/PDIS-08-13-0858-PDN .

Milosavljević, A., Pfaf-Dolovac, Erika, Mitrović, M., Jović, Jelena, Toševski, Ivo, Duduk, Nataša, Trkulja, Nenad, "First Report of Cercospora carotae, Causal Agent of Cercospora Leaf Spot of Carrot, in Serbia." in Plant Disease, 98, no. 8 (2014):1153-1153,
https://doi.org/10.1094/PDIS-08-13-0858-PDN . .

TY  - JOUR
AU  - Milosavljević, A.
AU  - Pfaf-Dolovac, Erika
AU  - Mitrović, M.
AU  - Jović, Jelena
AU  - Toševski, Ivo
AU  - Duduk, Nataša
AU  - Trkulja, Nenad
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3425
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Cercospora apii, Causal Agent of Cercospora Early Blight of Celery, in Serbia
EP  - 1157
IS  - 8
SP  - 1157
VL  - 98
DO  - 10.1094/PDIS-02-14-0135-PDN
ER  - 

@article{
author = "Milosavljević, A. and Pfaf-Dolovac, Erika and Mitrović, M. and Jović, Jelena and Toševski, Ivo and Duduk, Nataša and Trkulja, Nenad",
year = "2014",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Cercospora apii, Causal Agent of Cercospora Early Blight of Celery, in Serbia",
pages = "1157-1157",
number = "8",
volume = "98",
doi = "10.1094/PDIS-02-14-0135-PDN"
}

Milosavljević, A., Pfaf-Dolovac, E., Mitrović, M., Jović, J., Toševski, I., Duduk, N.,& Trkulja, N.. (2014). First Report of Cercospora apii, Causal Agent of Cercospora Early Blight of Celery, in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 98(8), 1157-1157.
https://doi.org/10.1094/PDIS-02-14-0135-PDN

Milosavljević A, Pfaf-Dolovac E, Mitrović M, Jović J, Toševski I, Duduk N, Trkulja N. First Report of Cercospora apii, Causal Agent of Cercospora Early Blight of Celery, in Serbia. in Plant Disease. 2014;98(8):1157-1157.
doi:10.1094/PDIS-02-14-0135-PDN .

Milosavljević, A., Pfaf-Dolovac, Erika, Mitrović, M., Jović, Jelena, Toševski, Ivo, Duduk, Nataša, Trkulja, Nenad, "First Report of Cercospora apii, Causal Agent of Cercospora Early Blight of Celery, in Serbia" in Plant Disease, 98, no. 8 (2014):1157-1157,
https://doi.org/10.1094/PDIS-02-14-0135-PDN . .

TY  - JOUR
AU  - Milosavljević, Anja
AU  - Pfaf-Dolovac, Erika
AU  - Mitrović, Milana
AU  - Jović, Jelena
AU  - Toševski, Ivo
AU  - Duduk, Nataša
AU  - Trkulja, Nenad
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3621
AB  - C. apii is one of the most damaging plant pathogenic fungi of celery. During the 2012, samples of celery with typical symptoms of leaf blight were collected on the territory of Veliko Grdaište and their identification is performed using morphological and molecular methods. The pathogenicity test was conducted on healthy plants and all tested isolates caused typical symptoms after inoculation. Conidiophores arising from the stromata formed dense fascicles or single, straight to slightly curved, brown. Conidia are solitary, hyaline and septate (5-13 septa), cylindrical to obclavate, or straight to slightly curved, obtuse at the apex. Truncated and thickened at the base. Fragments of the expected length for the internal transcribed spacer (ITS1/ITS4), calmodulin (CAL-228F/CAL2Rd) and histone (CYLH3F/ CYLH3R) gene were amplified and the obtained sequences showed 100% identity with the C. apii sequences deposited in GenBank.
AB  - C. apii predstavlja jednu od najštetnijih fitopatogenih gljiva celera. Tokom 2012. god na teritoriji Velikog Gradišta su prikupljeni uzorci celera sa karakterističnim simptomima pegavosti lista i njihova identifikacija je izvršena pomoću morfoloških i molekularnih metoda. Test patogenosti je obavljen na zdravim biljkama i svi ispitivani izolati prouzrokuju karakteristične simptome nakon inokulacije. Konidiofore iz stroma formiraju zbijene fascikule ili su pojedinačne, prave do blago zakrivljene, smeđe boje. Konidije su pojedinačne, hijalinske i septirane (5-13 septi), cilindrične do blago sužene na jednoj strani ili prave do blago zakrivljene i zatupaste na vrhu. U osnovi su zasečene, skoro kvadratnog oblika i stanjene. Amplifikovani su fragmenti očekivanih dužina za internal transcribed spacer (ITS1/ITS4), kalmodulin (CAL-228F/CAL2Rd) i histon (CYLH3F/CYLH3R) genima i dobijene sekvence su pokazale 100% identitet sa sekvencama C. apii u bazi gena.
PB  - Institut za zaštitu bilja i životnu sredinu, Beograd
T2  - Zaštita bilja
T1  - Morphological and molecular identification of Cercospora apii on celery in Serbia
T1  - Morfološka i molekularna identifikacija Cercospora apii na celeru u Srbiji
EP  - 84
IS  - 2
SP  - 77
VL  - 65
DO  - 10.5937/zasbilj1402077M
ER  - 

@article{
author = "Milosavljević, Anja and Pfaf-Dolovac, Erika and Mitrović, Milana and Jović, Jelena and Toševski, Ivo and Duduk, Nataša and Trkulja, Nenad",
year = "2014",
abstract = "C. apii is one of the most damaging plant pathogenic fungi of celery. During the 2012, samples of celery with typical symptoms of leaf blight were collected on the territory of Veliko Grdaište and their identification is performed using morphological and molecular methods. The pathogenicity test was conducted on healthy plants and all tested isolates caused typical symptoms after inoculation. Conidiophores arising from the stromata formed dense fascicles or single, straight to slightly curved, brown. Conidia are solitary, hyaline and septate (5-13 septa), cylindrical to obclavate, or straight to slightly curved, obtuse at the apex. Truncated and thickened at the base. Fragments of the expected length for the internal transcribed spacer (ITS1/ITS4), calmodulin (CAL-228F/CAL2Rd) and histone (CYLH3F/ CYLH3R) gene were amplified and the obtained sequences showed 100% identity with the C. apii sequences deposited in GenBank., C. apii predstavlja jednu od najštetnijih fitopatogenih gljiva celera. Tokom 2012. god na teritoriji Velikog Gradišta su prikupljeni uzorci celera sa karakterističnim simptomima pegavosti lista i njihova identifikacija je izvršena pomoću morfoloških i molekularnih metoda. Test patogenosti je obavljen na zdravim biljkama i svi ispitivani izolati prouzrokuju karakteristične simptome nakon inokulacije. Konidiofore iz stroma formiraju zbijene fascikule ili su pojedinačne, prave do blago zakrivljene, smeđe boje. Konidije su pojedinačne, hijalinske i septirane (5-13 septi), cilindrične do blago sužene na jednoj strani ili prave do blago zakrivljene i zatupaste na vrhu. U osnovi su zasečene, skoro kvadratnog oblika i stanjene. Amplifikovani su fragmenti očekivanih dužina za internal transcribed spacer (ITS1/ITS4), kalmodulin (CAL-228F/CAL2Rd) i histon (CYLH3F/CYLH3R) genima i dobijene sekvence su pokazale 100% identitet sa sekvencama C. apii u bazi gena.",
publisher = "Institut za zaštitu bilja i životnu sredinu, Beograd",
journal = "Zaštita bilja",
title = "Morphological and molecular identification of Cercospora apii on celery in Serbia, Morfološka i molekularna identifikacija Cercospora apii na celeru u Srbiji",
pages = "84-77",
number = "2",
volume = "65",
doi = "10.5937/zasbilj1402077M"
}

Milosavljević, A., Pfaf-Dolovac, E., Mitrović, M., Jović, J., Toševski, I., Duduk, N.,& Trkulja, N.. (2014). Morphological and molecular identification of Cercospora apii on celery in Serbia. in Zaštita bilja
Institut za zaštitu bilja i životnu sredinu, Beograd., 65(2), 77-84.
https://doi.org/10.5937/zasbilj1402077M

Milosavljević A, Pfaf-Dolovac E, Mitrović M, Jović J, Toševski I, Duduk N, Trkulja N. Morphological and molecular identification of Cercospora apii on celery in Serbia. in Zaštita bilja. 2014;65(2):77-84.
doi:10.5937/zasbilj1402077M .

Milosavljević, Anja, Pfaf-Dolovac, Erika, Mitrović, Milana, Jović, Jelena, Toševski, Ivo, Duduk, Nataša, Trkulja, Nenad, "Morphological and molecular identification of Cercospora apii on celery in Serbia" in Zaštita bilja, 65, no. 2 (2014):77-84,
https://doi.org/10.5937/zasbilj1402077M . .

TY  - JOUR
AU  - Lalević, Blažo
AU  - Raičević, Vera
AU  - Kiković, Dragan
AU  - Jovanović, L.
AU  - Šurlan-Momirović, Gordana
AU  - Jović, Jelena
AU  - Talaie, A.R.
AU  - Morina, Filis
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2816
AB  - Methyl tertiary butyl ether (MTBE) belongs to the group of gasoline oxygenates and persistent environment contaminants, and shows potential for biodegradation in aerobic and anaerobic conditions, through application of pure microbial cultures. Presented research shows that indigenous bacterial isolates 6sy and 24p, selected from oil hydrocarbons-contaminated environments, were capable of utilizing MTBE as sole carbon and energy source. Based on 16S rDNA sequence analysis, bacterial isolates 6sy and 24p were identified as Staphylococcus saprophyticus subsp. saprophyticus and Pseudomonas sp., respectively. The MTBE biodegradation rate was affected by longevity of incubation period and initial MTBE concentration. After 3 weeks of incubation at 25°C in a dark, the removal rates of initial 25 and 125 ppm MTBE concentrations by Staphylococcus saprophyticus 6sy were found to be 97, and 63%, respectively, while efficiency of Pseudomonas sp. in degradation of indicated concentrations was 96, and 40%, respectively. Both bacterial isolates were able to grow in MTBE-containing growth medium. Highest growth rate of bacterial isolates was observed at the end of incubation period. The presented results indicated the potential of these bacterial isolates in bioremediation of MTBE-contaminated environments.
T2  - International Journal of Environmental Research
T1  - Biodegradation of MTBE by bacteria isolated from oil hydrocarbons - Contaminated environments
EP  - 86
IS  - 1
SP  - 81
VL  - 6
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_2816
ER  - 

@article{
author = "Lalević, Blažo and Raičević, Vera and Kiković, Dragan and Jovanović, L. and Šurlan-Momirović, Gordana and Jović, Jelena and Talaie, A.R. and Morina, Filis",
year = "2012",
abstract = "Methyl tertiary butyl ether (MTBE) belongs to the group of gasoline oxygenates and persistent environment contaminants, and shows potential for biodegradation in aerobic and anaerobic conditions, through application of pure microbial cultures. Presented research shows that indigenous bacterial isolates 6sy and 24p, selected from oil hydrocarbons-contaminated environments, were capable of utilizing MTBE as sole carbon and energy source. Based on 16S rDNA sequence analysis, bacterial isolates 6sy and 24p were identified as Staphylococcus saprophyticus subsp. saprophyticus and Pseudomonas sp., respectively. The MTBE biodegradation rate was affected by longevity of incubation period and initial MTBE concentration. After 3 weeks of incubation at 25°C in a dark, the removal rates of initial 25 and 125 ppm MTBE concentrations by Staphylococcus saprophyticus 6sy were found to be 97, and 63%, respectively, while efficiency of Pseudomonas sp. in degradation of indicated concentrations was 96, and 40%, respectively. Both bacterial isolates were able to grow in MTBE-containing growth medium. Highest growth rate of bacterial isolates was observed at the end of incubation period. The presented results indicated the potential of these bacterial isolates in bioremediation of MTBE-contaminated environments.",
journal = "International Journal of Environmental Research",
title = "Biodegradation of MTBE by bacteria isolated from oil hydrocarbons - Contaminated environments",
pages = "86-81",
number = "1",
volume = "6",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_2816"
}

Lalević, B., Raičević, V., Kiković, D., Jovanović, L., Šurlan-Momirović, G., Jović, J., Talaie, A.R.,& Morina, F.. (2012). Biodegradation of MTBE by bacteria isolated from oil hydrocarbons - Contaminated environments. in International Journal of Environmental Research, 6(1), 81-86.
https://hdl.handle.net/21.15107/rcub_agrospace_2816

Lalević B, Raičević V, Kiković D, Jovanović L, Šurlan-Momirović G, Jović J, Talaie A, Morina F. Biodegradation of MTBE by bacteria isolated from oil hydrocarbons - Contaminated environments. in International Journal of Environmental Research. 2012;6(1):81-86.
https://hdl.handle.net/21.15107/rcub_agrospace_2816 .

Lalević, Blažo, Raičević, Vera, Kiković, Dragan, Jovanović, L., Šurlan-Momirović, Gordana, Jović, Jelena, Talaie, A.R., Morina, Filis, "Biodegradation of MTBE by bacteria isolated from oil hydrocarbons - Contaminated environments" in International Journal of Environmental Research, 6, no. 1 (2012):81-86,
https://hdl.handle.net/21.15107/rcub_agrospace_2816 .

TY  - JOUR
AU  - Lalević, Blažo
AU  - Jović, Jelena
AU  - Raičević, Vera
AU  - Kljujev, Igor
AU  - Kiković, Dragan
AU  - Hamidović, Saud
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2922
AB  - Methyl-tert-butyl ether (MTBE) has been used to replace the toxic compounds from gasoline and to reduce emission of air pollutants. Due to its intensive use, MTBE has become one of the most important environment pollutants. The aim of this study was the isolation and identification of the bacteria from wastewater sample of 'HIP Petrohemija', Pančevo (Serbia), capable of MTBE biodegradation. The results of the investigation showed that only the bacterial isolate 27/1 was capable of growth on MTBE. The result of sequence analyzes of 16S rDNA showed that this bacterial isolate belongs to the Kocuria sp. After the incubation period of 86 days, the degradation rates of initial MTBE concentration of 25 and 125 μg/ml were 55 and 36%, respectively. These results indicated that bacteria Kocuria sp. is successfully adapted on MTBE and can be potentially used in bioremediation of soils and waters contaminated with MTBE.
AB  - Metil tercijarni butil etar (MTBE) uveden je u proizvodnju s ciljem zamene toksičnih komponenti iz benzina, povećanja oktanskog broja goriva i redukcije aerozagađenja. Usled intenzivne primene, perzistentnosti i mobilnosti, MTBE je posle svega nekoliko godina postao značajan polutant u životnoj sredini. Cilj ovog rada je izolacija i identifikacija bakterija iz uzorka otpadne vode poreklom iz 'HIP Petrohemije', Pančevo, sposobnih da vrše degradaciju MTBE-a. Od 11 bakterijskih izolata, svrstanih u tri grupe po morfološkim osobinama, samo je jedan izolat, označen kao 27/1, pokazao sposobnost rasta na MTBE-u kao jedinstvenom izvoru ugljenika i energije i odabran je za dalja istraživanja. Ovaj izolat ima sitne crvenkaste kolonije na podlozi 0,1×TSA. Ćelije su okruglastog oblika, ne stvaraju spore a po Gramu se boje pozitivno. Primenom API i APIWEB sistema, ovaj izolat pokazuje najveći stepen sličnosti sa bakterijskom vrstom Kocuria rosea. Sekvenca izolata 27/1 dobijena sekvencionom 16S rDNA analizom se sastoji od 1407 nukleotida, na osnovu čega je ovaj izolat identifikovan kao Kocuria sp. Nakon inkubacije od 86 dana, stepen degradacije početnih koncentracija MTBE-a od 25 i 125 μg/ml iznosio je 55, odnosno 36%. Pri početnoj koncentraciji od 25 μg/ml optička gustina i brojnost bakterija se smanjuju u prvih 15 dana inkubacije, dok je pri početnoj koncentraciji od 125 μg/ml zabeleženo smanjenje optičke gustine i broja bakterija u prvih 30 dana. Nakon ovog perioda, stepen bakterijskog rasta se povećava. U početnim fazama Inkubacije, stepen degradacije MTBE-a nije visok, dok je krajem inkubacionog perioda konstatovan najveći stepen degradacije. Ovi rezultati ukazuju na uspešnu adaptaciju bakterije Kocuria sp. na prisustvo MTBE-a, što opravdava njenu primenu u bioremedijaciji zemljišta i voda kontaminiranih MTBE-om.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska industrija
T1  - Biodegradation of methyl-tert-butyl ether by Kocuria sp.
T1  - Biodegradacija metil tercijarnog butil etra pomoću Kocuria sp.
EP  - 722
IS  - 5
SP  - 717
VL  - 66
DO  - 10.2298/HEMIND120110019L
ER  - 

@article{
author = "Lalević, Blažo and Jović, Jelena and Raičević, Vera and Kljujev, Igor and Kiković, Dragan and Hamidović, Saud",
year = "2012",
abstract = "Methyl-tert-butyl ether (MTBE) has been used to replace the toxic compounds from gasoline and to reduce emission of air pollutants. Due to its intensive use, MTBE has become one of the most important environment pollutants. The aim of this study was the isolation and identification of the bacteria from wastewater sample of 'HIP Petrohemija', Pančevo (Serbia), capable of MTBE biodegradation. The results of the investigation showed that only the bacterial isolate 27/1 was capable of growth on MTBE. The result of sequence analyzes of 16S rDNA showed that this bacterial isolate belongs to the Kocuria sp. After the incubation period of 86 days, the degradation rates of initial MTBE concentration of 25 and 125 μg/ml were 55 and 36%, respectively. These results indicated that bacteria Kocuria sp. is successfully adapted on MTBE and can be potentially used in bioremediation of soils and waters contaminated with MTBE., Metil tercijarni butil etar (MTBE) uveden je u proizvodnju s ciljem zamene toksičnih komponenti iz benzina, povećanja oktanskog broja goriva i redukcije aerozagađenja. Usled intenzivne primene, perzistentnosti i mobilnosti, MTBE je posle svega nekoliko godina postao značajan polutant u životnoj sredini. Cilj ovog rada je izolacija i identifikacija bakterija iz uzorka otpadne vode poreklom iz 'HIP Petrohemije', Pančevo, sposobnih da vrše degradaciju MTBE-a. Od 11 bakterijskih izolata, svrstanih u tri grupe po morfološkim osobinama, samo je jedan izolat, označen kao 27/1, pokazao sposobnost rasta na MTBE-u kao jedinstvenom izvoru ugljenika i energije i odabran je za dalja istraživanja. Ovaj izolat ima sitne crvenkaste kolonije na podlozi 0,1×TSA. Ćelije su okruglastog oblika, ne stvaraju spore a po Gramu se boje pozitivno. Primenom API i APIWEB sistema, ovaj izolat pokazuje najveći stepen sličnosti sa bakterijskom vrstom Kocuria rosea. Sekvenca izolata 27/1 dobijena sekvencionom 16S rDNA analizom se sastoji od 1407 nukleotida, na osnovu čega je ovaj izolat identifikovan kao Kocuria sp. Nakon inkubacije od 86 dana, stepen degradacije početnih koncentracija MTBE-a od 25 i 125 μg/ml iznosio je 55, odnosno 36%. Pri početnoj koncentraciji od 25 μg/ml optička gustina i brojnost bakterija se smanjuju u prvih 15 dana inkubacije, dok je pri početnoj koncentraciji od 125 μg/ml zabeleženo smanjenje optičke gustine i broja bakterija u prvih 30 dana. Nakon ovog perioda, stepen bakterijskog rasta se povećava. U početnim fazama Inkubacije, stepen degradacije MTBE-a nije visok, dok je krajem inkubacionog perioda konstatovan najveći stepen degradacije. Ovi rezultati ukazuju na uspešnu adaptaciju bakterije Kocuria sp. na prisustvo MTBE-a, što opravdava njenu primenu u bioremedijaciji zemljišta i voda kontaminiranih MTBE-om.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Biodegradation of methyl-tert-butyl ether by Kocuria sp., Biodegradacija metil tercijarnog butil etra pomoću Kocuria sp.",
pages = "722-717",
number = "5",
volume = "66",
doi = "10.2298/HEMIND120110019L"
}

Lalević, B., Jović, J., Raičević, V., Kljujev, I., Kiković, D.,& Hamidović, S.. (2012). Biodegradation of methyl-tert-butyl ether by Kocuria sp.. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 66(5), 717-722.
https://doi.org/10.2298/HEMIND120110019L

Lalević B, Jović J, Raičević V, Kljujev I, Kiković D, Hamidović S. Biodegradation of methyl-tert-butyl ether by Kocuria sp.. in Hemijska industrija. 2012;66(5):717-722.
doi:10.2298/HEMIND120110019L .

Lalević, Blažo, Jović, Jelena, Raičević, Vera, Kljujev, Igor, Kiković, Dragan, Hamidović, Saud, "Biodegradation of methyl-tert-butyl ether by Kocuria sp." in Hemijska industrija, 66, no. 5 (2012):717-722,
https://doi.org/10.2298/HEMIND120110019L . .

TY  - JOUR
AU  - Vučurović, Ana
AU  - Bulajić, Aleksandra
AU  - Stanković, Ivana
AU  - Ristić, Danijela
AU  - Berenji, Janoš
AU  - Jović, Jelena
AU  - Krstić, Branka
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2961
AB  - Cucurbit species grown in the Vojvodina Province, Serbia, were surveyed for the incidence of Zucchini yellow mosaic virus (ZYMV), Watermelon mosaic virus (WMV), Cucumber mosaic virus (CMV), Squash mosaic virus (SqMV), Papaya ringspot virus (PRSV) and Tobacco ringspot virus (TRSV) from 2007 to 2009. Samples from more than 700 pumpkin, squash and bottle gourd plants with virus-like symptoms were analyzed by double-antibody sandwich (DAS)-ELISA. ZYMV, WMV and CMV were detected in 79.2, 32.2, and 12.8% of tested samples, respectively. WMV was prevalent in 2007 and ZYMV in 2008-09. Mixed infections were the most frequent type in 2007-08 in contrast to 2009 when single infection of ZYMV prevailed. ZYMV was the most widespread being found in 33 out of 39 inspected fields. Virus species identification was confirmed in selected samples by conventional reverse transcription-polymerase chain reaction (RT-PCR) and sequencing of their coat protein genes. By comparing the obtained virus isolate sequences with those available in GenBank, the identification of serologically detected viruses was confirmed. Phylogenetic analysis based on complete coat protein (CP) sequences highlighted that Serbian ZYMV isolates were closely related to other Central European ZYMV isolates. Finally, additional testing of ELISA-negative samples by RT-PCR using primers specific to six other mosaic viruses revealed the presence of Tomato spotted wilt virus (TSWV) in winter (Cucurbita maxima) and summer (C. pepo 'Beogradska') squash. This is the first report of TSWV natural occurrence on cucurbits in Serbia and on winter squash worldwide.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Non-persistently aphid-borne viruses infecting pumpkin and squash in Serbia and partial characterization of Zucchini yellow mosaic virus isolates
EP  - 947
IS  - 4
SP  - 935
VL  - 133
DO  - 10.1007/s10658-012-9964-x
ER  - 

@article{
author = "Vučurović, Ana and Bulajić, Aleksandra and Stanković, Ivana and Ristić, Danijela and Berenji, Janoš and Jović, Jelena and Krstić, Branka",
year = "2012",
abstract = "Cucurbit species grown in the Vojvodina Province, Serbia, were surveyed for the incidence of Zucchini yellow mosaic virus (ZYMV), Watermelon mosaic virus (WMV), Cucumber mosaic virus (CMV), Squash mosaic virus (SqMV), Papaya ringspot virus (PRSV) and Tobacco ringspot virus (TRSV) from 2007 to 2009. Samples from more than 700 pumpkin, squash and bottle gourd plants with virus-like symptoms were analyzed by double-antibody sandwich (DAS)-ELISA. ZYMV, WMV and CMV were detected in 79.2, 32.2, and 12.8% of tested samples, respectively. WMV was prevalent in 2007 and ZYMV in 2008-09. Mixed infections were the most frequent type in 2007-08 in contrast to 2009 when single infection of ZYMV prevailed. ZYMV was the most widespread being found in 33 out of 39 inspected fields. Virus species identification was confirmed in selected samples by conventional reverse transcription-polymerase chain reaction (RT-PCR) and sequencing of their coat protein genes. By comparing the obtained virus isolate sequences with those available in GenBank, the identification of serologically detected viruses was confirmed. Phylogenetic analysis based on complete coat protein (CP) sequences highlighted that Serbian ZYMV isolates were closely related to other Central European ZYMV isolates. Finally, additional testing of ELISA-negative samples by RT-PCR using primers specific to six other mosaic viruses revealed the presence of Tomato spotted wilt virus (TSWV) in winter (Cucurbita maxima) and summer (C. pepo 'Beogradska') squash. This is the first report of TSWV natural occurrence on cucurbits in Serbia and on winter squash worldwide.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Non-persistently aphid-borne viruses infecting pumpkin and squash in Serbia and partial characterization of Zucchini yellow mosaic virus isolates",
pages = "947-935",
number = "4",
volume = "133",
doi = "10.1007/s10658-012-9964-x"
}

Vučurović, A., Bulajić, A., Stanković, I., Ristić, D., Berenji, J., Jović, J.,& Krstić, B.. (2012). Non-persistently aphid-borne viruses infecting pumpkin and squash in Serbia and partial characterization of Zucchini yellow mosaic virus isolates. in European Journal of Plant Pathology
Springer, Dordrecht., 133(4), 935-947.
https://doi.org/10.1007/s10658-012-9964-x

Vučurović A, Bulajić A, Stanković I, Ristić D, Berenji J, Jović J, Krstić B. Non-persistently aphid-borne viruses infecting pumpkin and squash in Serbia and partial characterization of Zucchini yellow mosaic virus isolates. in European Journal of Plant Pathology. 2012;133(4):935-947.
doi:10.1007/s10658-012-9964-x .

Vučurović, Ana, Bulajić, Aleksandra, Stanković, Ivana, Ristić, Danijela, Berenji, Janoš, Jović, Jelena, Krstić, Branka, "Non-persistently aphid-borne viruses infecting pumpkin and squash in Serbia and partial characterization of Zucchini yellow mosaic virus isolates" in European Journal of Plant Pathology, 133, no. 4 (2012):935-947,
https://doi.org/10.1007/s10658-012-9964-x . .

TY  - JOUR
AU  - Lalević, Blažo
AU  - Raičević, Vera
AU  - Kiković, Dragan
AU  - Jovanović, L.
AU  - Šurlan-Momirović, Gordana
AU  - Jović, Jelena
AU  - Talaie, A.R.
AU  - Morina, Filis
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2442
AB  - Methyl tertiary butyl ether (MTBE) belongs to the group of gasoline oxygenates and persistent environment contaminants, and shows potential for biodegradation in aerobic and anaerobic conditions, through application of pure microbial cultures. Presented research shows that indigenous bacterial isolates 6sy and 24p, selected from oil hydrocarbons-contaminated environments, were capable of utilizing MTBE as sole carbon and energy source. Based on 16S rDNA sequence analysis, bacterial isolates 6sy and 24p were identified as Staphylococcus saprophyticus subsp. saprophyticus and Pseudomonas sp., respectively. The MTBE biodegradation rate was affected by longevity of incubation period and initial MTBE concentration. After 3 weeks of incubation at 25 degrees C in a dark, the removal rates of initial 25 and 125 ppm MTBE concentrations by Staphylococcus saprophyticus 6sy were found to be 97, and 63%, respectively, while efficiency of Pseudomonas sp. in degradation of indicated concentrations was 96, and 40%, respectively. Both bacterial isolates were able to grow in MTBE-containing growth medium. Highest growth rate of bacterial isolates was observed at the end of incubation period. The presented results indicated the potential of these bacterial isolates in bioremediation of MTBE-contaminated environments.
T2  - International Journal of Environmental Research
T1  - Biodegradation of MTBE by Bacteria Isolated from oil Hydrocarbons-Contaminated Environments
EP  - 832
IS  - 4
SP  - 827
VL  - 5
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_2442
ER  - 

@article{
author = "Lalević, Blažo and Raičević, Vera and Kiković, Dragan and Jovanović, L. and Šurlan-Momirović, Gordana and Jović, Jelena and Talaie, A.R. and Morina, Filis",
year = "2011",
abstract = "Methyl tertiary butyl ether (MTBE) belongs to the group of gasoline oxygenates and persistent environment contaminants, and shows potential for biodegradation in aerobic and anaerobic conditions, through application of pure microbial cultures. Presented research shows that indigenous bacterial isolates 6sy and 24p, selected from oil hydrocarbons-contaminated environments, were capable of utilizing MTBE as sole carbon and energy source. Based on 16S rDNA sequence analysis, bacterial isolates 6sy and 24p were identified as Staphylococcus saprophyticus subsp. saprophyticus and Pseudomonas sp., respectively. The MTBE biodegradation rate was affected by longevity of incubation period and initial MTBE concentration. After 3 weeks of incubation at 25 degrees C in a dark, the removal rates of initial 25 and 125 ppm MTBE concentrations by Staphylococcus saprophyticus 6sy were found to be 97, and 63%, respectively, while efficiency of Pseudomonas sp. in degradation of indicated concentrations was 96, and 40%, respectively. Both bacterial isolates were able to grow in MTBE-containing growth medium. Highest growth rate of bacterial isolates was observed at the end of incubation period. The presented results indicated the potential of these bacterial isolates in bioremediation of MTBE-contaminated environments.",
journal = "International Journal of Environmental Research",
title = "Biodegradation of MTBE by Bacteria Isolated from oil Hydrocarbons-Contaminated Environments",
pages = "832-827",
number = "4",
volume = "5",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_2442"
}

Lalević, B., Raičević, V., Kiković, D., Jovanović, L., Šurlan-Momirović, G., Jović, J., Talaie, A.R.,& Morina, F.. (2011). Biodegradation of MTBE by Bacteria Isolated from oil Hydrocarbons-Contaminated Environments. in International Journal of Environmental Research, 5(4), 827-832.
https://hdl.handle.net/21.15107/rcub_agrospace_2442

Lalević B, Raičević V, Kiković D, Jovanović L, Šurlan-Momirović G, Jović J, Talaie A, Morina F. Biodegradation of MTBE by Bacteria Isolated from oil Hydrocarbons-Contaminated Environments. in International Journal of Environmental Research. 2011;5(4):827-832.
https://hdl.handle.net/21.15107/rcub_agrospace_2442 .

Lalević, Blažo, Raičević, Vera, Kiković, Dragan, Jovanović, L., Šurlan-Momirović, Gordana, Jović, Jelena, Talaie, A.R., Morina, Filis, "Biodegradation of MTBE by Bacteria Isolated from oil Hydrocarbons-Contaminated Environments" in International Journal of Environmental Research, 5, no. 4 (2011):827-832,
https://hdl.handle.net/21.15107/rcub_agrospace_2442 .

TY  - JOUR
AU  - Bulajić, A.
AU  - Vučurović, A.
AU  - Stanković, I.
AU  - Ristić, D.
AU  - Jović, J.
AU  - Stojković, B.
AU  - Krstić, B.
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2724
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Plasmopara obducens on Impatiens walleriana in Serbia
EP  - 491
IS  - 4
SP  - 491
VL  - 95
DO  - 10.1094/PDIS-10-10-0731
ER  - 

@article{
author = "Bulajić, A. and Vučurović, A. and Stanković, I. and Ristić, D. and Jović, J. and Stojković, B. and Krstić, B.",
year = "2011",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Plasmopara obducens on Impatiens walleriana in Serbia",
pages = "491-491",
number = "4",
volume = "95",
doi = "10.1094/PDIS-10-10-0731"
}

Bulajić, A., Vučurović, A., Stanković, I., Ristić, D., Jović, J., Stojković, B.,& Krstić, B.. (2011). First Report of Plasmopara obducens on Impatiens walleriana in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 95(4), 491-491.
https://doi.org/10.1094/PDIS-10-10-0731

Bulajić A, Vučurović A, Stanković I, Ristić D, Jović J, Stojković B, Krstić B. First Report of Plasmopara obducens on Impatiens walleriana in Serbia. in Plant Disease. 2011;95(4):491-491.
doi:10.1094/PDIS-10-10-0731 .

Bulajić, A., Vučurović, A., Stanković, I., Ristić, D., Jović, J., Stojković, B., Krstić, B., "First Report of Plasmopara obducens on Impatiens walleriana in Serbia" in Plant Disease, 95, no. 4 (2011):491-491,
https://doi.org/10.1094/PDIS-10-10-0731 . .

TY  - JOUR
AU  - Stanković, Ivana
AU  - Bulajić, Aleksandra
AU  - Vučurović, Ana
AU  - Ristić, Danijela
AU  - Jović, Jelena
AU  - Krstić, Branka
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2685
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Tomato spotted wilt virus on Gerbera hybrida in Serbia.
EP  - 226
IS  - 2
SP  - 226
VL  - 95
DO  - 10.1094/PDIS-10-10-0704
ER  - 

@article{
author = "Stanković, Ivana and Bulajić, Aleksandra and Vučurović, Ana and Ristić, Danijela and Jović, Jelena and Krstić, Branka",
year = "2011",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Tomato spotted wilt virus on Gerbera hybrida in Serbia.",
pages = "226-226",
number = "2",
volume = "95",
doi = "10.1094/PDIS-10-10-0704"
}

Stanković, I., Bulajić, A., Vučurović, A., Ristić, D., Jović, J.,& Krstić, B.. (2011). First Report of Tomato spotted wilt virus on Gerbera hybrida in Serbia.. in Plant Disease
Amer Phytopathological Soc, St Paul., 95(2), 226-226.
https://doi.org/10.1094/PDIS-10-10-0704

Stanković I, Bulajić A, Vučurović A, Ristić D, Jović J, Krstić B. First Report of Tomato spotted wilt virus on Gerbera hybrida in Serbia.. in Plant Disease. 2011;95(2):226-226.
doi:10.1094/PDIS-10-10-0704 .

Stanković, Ivana, Bulajić, Aleksandra, Vučurović, Ana, Ristić, Danijela, Jović, Jelena, Krstić, Branka, "First Report of Tomato spotted wilt virus on Gerbera hybrida in Serbia." in Plant Disease, 95, no. 2 (2011):226-226,
https://doi.org/10.1094/PDIS-10-10-0704 . .

TY  - JOUR
AU  - Vučurović, Ana
AU  - Bulajić, Aleksandra
AU  - Stanković, Ivana
AU  - Ristić, Danijela
AU  - Berenji, Janoš
AU  - Jović, Jelena
AU  - Krstić, Branka
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2663
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of the Occurrence of Cucurbit aphid-borne yellows virus on Oilseed Pumpkin in Serbia
EP  - 1035
IS  - 8
SP  - 1035
VL  - 95
DO  - 10.1094/PDIS-02-11-0147
ER  - 

@article{
author = "Vučurović, Ana and Bulajić, Aleksandra and Stanković, Ivana and Ristić, Danijela and Berenji, Janoš and Jović, Jelena and Krstić, Branka",
year = "2011",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of the Occurrence of Cucurbit aphid-borne yellows virus on Oilseed Pumpkin in Serbia",
pages = "1035-1035",
number = "8",
volume = "95",
doi = "10.1094/PDIS-02-11-0147"
}

Vučurović, A., Bulajić, A., Stanković, I., Ristić, D., Berenji, J., Jović, J.,& Krstić, B.. (2011). First Report of the Occurrence of Cucurbit aphid-borne yellows virus on Oilseed Pumpkin in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 95(8), 1035-1035.
https://doi.org/10.1094/PDIS-02-11-0147

Vučurović A, Bulajić A, Stanković I, Ristić D, Berenji J, Jović J, Krstić B. First Report of the Occurrence of Cucurbit aphid-borne yellows virus on Oilseed Pumpkin in Serbia. in Plant Disease. 2011;95(8):1035-1035.
doi:10.1094/PDIS-02-11-0147 .

Vučurović, Ana, Bulajić, Aleksandra, Stanković, Ivana, Ristić, Danijela, Berenji, Janoš, Jović, Jelena, Krstić, Branka, "First Report of the Occurrence of Cucurbit aphid-borne yellows virus on Oilseed Pumpkin in Serbia" in Plant Disease, 95, no. 8 (2011):1035-1035,
https://doi.org/10.1094/PDIS-02-11-0147 . .

TY  - JOUR
AU  - Ristić, Danijela
AU  - Vučurović, Ana
AU  - Stanković, Ivana
AU  - Jović, Jelena
AU  - Milojević, Katarina
AU  - Bulajić, Aleksandra
AU  - Krstić, Branka
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2518
AB  - During 2010, Impatiens walleriana plants with symptoms of downy mildew were collected in a greenhouse in the vicinity of Mionica, Kolubara District. Disease incidence was extremely high, approaching 100%, and wilting and collapse of affected plants was very rapid, resulting in losses of more than 90%. White downy growth produced on the lower leaf surface consisted of hyaline, thin-walled sporangiophores with monopodial branching and numerous, ovoid and hyaline sporangia. Apical branchlets of sporangiophores were at right angles to the main axis, with no apical thickening. Pathogenicity tests included inoculation of young I. walleriana plants by spraying with a sporangial suspension, and downy mildew symptoms were observed after 13 to 15 days. The absence of well-defined spots on the infected impatiens leaves and straight sporangiophores indicated that the pathogen was P. obducens, which was further supported by molecular identification, the 5'-end of the nuclear DNA coding for the large ribosomal subunit (LSU rDNA) was amplified by PCR, using primers NL1 and NL4. A representative isolate, 28-10, was sequenced and phylogenetic analysis showed its grouping with other P. obducens isolates of different origin. Considering that impatiens downy mildew in Serbia is proved to be caused by P. obducens it is necessary to employ adequate phytosanitary measures to prevent further spread of the pathogen.
AB  - Tokom 2010. godine biljke Impatiens walleriana sa simptomima plamenjače sakupljene su u stakleniku u okolini Mionice, Kolubarski okrug. Učestalost oboljenja bila je izuzetno visoka, blizu 100%, a sušenje i propadanje zaraženih biljaka veoma brzo, što je dovelo do šteta većih od 90%. Bela prevlaka patogena koja se razvijala na naličju listova sastojala se od hijalinskih monopodijalno razgranatih sporangiofora sa tankim zidovima i brojnih, ovoidnih i hijalinskih sporangija. Vrhovi grana sporangiofora granali su se pod pravim uglom u odnosu na glavnu osu, bez vršnog zadebljanja. Testovi patogenosti uključili su inokulacije mladih biljaka I. walleriana prskanjem suspenzijom sporangija, a simptomi plamenjače razvili su se nakon 13-15 dana. Odsustvo definisanih pega na zaraženim listovima impatiensa i prisustvo pravih sporangiofora ukazalo je da je prouzrokovač P. obducens, što je dalje potvrđeno molekularnom identifikacijom. Primenom lančane reakcije polimeraze (polymerase chain reaction, PCR), pomoću prajmera NL1 i NL4, umnožen je kodirajući 5'-kraj DNA velike ribozomalne podjedinice (LSU rDNA). Amplifikovani produkt odabranog izolata 28-10 je sekvencioniran, a filogenetske analize pokazale su njegovo grupisanje sa ostalim izolatima P. obducens različitog porekla. Uzimajući u obzir da je ovim istraživanjima dokazano da je prouzrokovač plamenjače na Impatiens walleriana u Srbiji P. obducens, neophodno je preduzimanje odgovarajućih fitosanitarnih mera u cilju sprečavanja daljeg širenja patogena u našoj zemlji.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Plasmopara obducens: A new threat to the production of Impatiens Walleriana in Serbia
T1  - Plasmopara obducens - nova pretnja proizvodnji Impatiens Walleriana u Srbiji
EP  - 53
IS  - 1
SP  - 43
VL  - 26
DO  - 10.2298/PIF1101043R
ER  - 

@article{
author = "Ristić, Danijela and Vučurović, Ana and Stanković, Ivana and Jović, Jelena and Milojević, Katarina and Bulajić, Aleksandra and Krstić, Branka",
year = "2011",
abstract = "During 2010, Impatiens walleriana plants with symptoms of downy mildew were collected in a greenhouse in the vicinity of Mionica, Kolubara District. Disease incidence was extremely high, approaching 100%, and wilting and collapse of affected plants was very rapid, resulting in losses of more than 90%. White downy growth produced on the lower leaf surface consisted of hyaline, thin-walled sporangiophores with monopodial branching and numerous, ovoid and hyaline sporangia. Apical branchlets of sporangiophores were at right angles to the main axis, with no apical thickening. Pathogenicity tests included inoculation of young I. walleriana plants by spraying with a sporangial suspension, and downy mildew symptoms were observed after 13 to 15 days. The absence of well-defined spots on the infected impatiens leaves and straight sporangiophores indicated that the pathogen was P. obducens, which was further supported by molecular identification, the 5'-end of the nuclear DNA coding for the large ribosomal subunit (LSU rDNA) was amplified by PCR, using primers NL1 and NL4. A representative isolate, 28-10, was sequenced and phylogenetic analysis showed its grouping with other P. obducens isolates of different origin. Considering that impatiens downy mildew in Serbia is proved to be caused by P. obducens it is necessary to employ adequate phytosanitary measures to prevent further spread of the pathogen., Tokom 2010. godine biljke Impatiens walleriana sa simptomima plamenjače sakupljene su u stakleniku u okolini Mionice, Kolubarski okrug. Učestalost oboljenja bila je izuzetno visoka, blizu 100%, a sušenje i propadanje zaraženih biljaka veoma brzo, što je dovelo do šteta većih od 90%. Bela prevlaka patogena koja se razvijala na naličju listova sastojala se od hijalinskih monopodijalno razgranatih sporangiofora sa tankim zidovima i brojnih, ovoidnih i hijalinskih sporangija. Vrhovi grana sporangiofora granali su se pod pravim uglom u odnosu na glavnu osu, bez vršnog zadebljanja. Testovi patogenosti uključili su inokulacije mladih biljaka I. walleriana prskanjem suspenzijom sporangija, a simptomi plamenjače razvili su se nakon 13-15 dana. Odsustvo definisanih pega na zaraženim listovima impatiensa i prisustvo pravih sporangiofora ukazalo je da je prouzrokovač P. obducens, što je dalje potvrđeno molekularnom identifikacijom. Primenom lančane reakcije polimeraze (polymerase chain reaction, PCR), pomoću prajmera NL1 i NL4, umnožen je kodirajući 5'-kraj DNA velike ribozomalne podjedinice (LSU rDNA). Amplifikovani produkt odabranog izolata 28-10 je sekvencioniran, a filogenetske analize pokazale su njegovo grupisanje sa ostalim izolatima P. obducens različitog porekla. Uzimajući u obzir da je ovim istraživanjima dokazano da je prouzrokovač plamenjače na Impatiens walleriana u Srbiji P. obducens, neophodno je preduzimanje odgovarajućih fitosanitarnih mera u cilju sprečavanja daljeg širenja patogena u našoj zemlji.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Plasmopara obducens: A new threat to the production of Impatiens Walleriana in Serbia, Plasmopara obducens - nova pretnja proizvodnji Impatiens Walleriana u Srbiji",
pages = "53-43",
number = "1",
volume = "26",
doi = "10.2298/PIF1101043R"
}

Ristić, D., Vučurović, A., Stanković, I., Jović, J., Milojević, K., Bulajić, A.,& Krstić, B.. (2011). Plasmopara obducens: A new threat to the production of Impatiens Walleriana in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 26(1), 43-53.
https://doi.org/10.2298/PIF1101043R

Ristić D, Vučurović A, Stanković I, Jović J, Milojević K, Bulajić A, Krstić B. Plasmopara obducens: A new threat to the production of Impatiens Walleriana in Serbia. in Pesticidi i fitomedicina. 2011;26(1):43-53.
doi:10.2298/PIF1101043R .

Ristić, Danijela, Vučurović, Ana, Stanković, Ivana, Jović, Jelena, Milojević, Katarina, Bulajić, Aleksandra, Krstić, Branka, "Plasmopara obducens: A new threat to the production of Impatiens Walleriana in Serbia" in Pesticidi i fitomedicina, 26, no. 1 (2011):43-53,
https://doi.org/10.2298/PIF1101043R . .

TY  - JOUR
AU  - Bulajić, Aleksandra
AU  - Djekić, Ivana
AU  - Jović, Jelena
AU  - Krnjajić, Slobodan
AU  - Vučurović, Ana
AU  - Krstić, Branka
PY  - 2010
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2314
AB  - In a survey to determine the presence of Phytophthora ramorum in Serbia, ornamentals from garden centers, nurseries, and private and public gardens, as well as imported plant material, were inspected. In total, 577 plant, soil, and potting media samples were tested using various detection methods: lateral flow diagnostic test, enzyme-linked immunosorbent assay, conventional polymerase chain reaction, and isolation, followed by identification based on growth characteristics in culture and morphological features. P. ramorum was not detected in any of the 162 soil or potting media tested by the baiting method. P. ramorum was detected in 12 Rhododendron samples from one private garden in Zemun (City of Belgrade District) exhibiting symptoms of leaf necrosis and blight and petiole necrosis, and in three samples of Pieris spp. from one garden center exhibiting symptoms of leaf necrosis. Eight Phytophthora isolates were obtained from the positive Rhododendron plants and three isolates from Pieris plants, and all were identified as P. ramorum on the basis of their uniform morphological and growth characteristics. P. ramorum conformation was also made by sequencing of the internal transcribed spacer regions for a single isolate taken from one infected rhododendron and one pieris plant. Serbian isolates were determined as A1 mating type, due to formation of a few typical sexual structures when crossed with the A2 mating type of P. cinnamomi and P. cryptogea. Pathogenicity test on non-wounded detached leaves of 19 popular ornamentals, as well as the most frequently imported ones, revealed that 10 host species were susceptible, including Robinia pseudoacacia, which is widely distributed in Serbia. During this study, Cotoneaster horizontalis and C. dammeri were determined to be new experimental hosts of P ramorum. This article provides evidence of P. ramorum introduction into Serbia. Although P. ramorum has not been detected in Serbian production nurseries, its presence outdoors might cause severe damages on susceptible common urban plants in public green and natural ecosystems.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Phytophthora ramorum Occurrence in Ornamentals in Serbia
EP  - 708
IS  - 6
SP  - 703
VL  - 94
DO  - 10.1094/PDIS-94-6-0703
ER  - 

@article{
author = "Bulajić, Aleksandra and Djekić, Ivana and Jović, Jelena and Krnjajić, Slobodan and Vučurović, Ana and Krstić, Branka",
year = "2010",
abstract = "In a survey to determine the presence of Phytophthora ramorum in Serbia, ornamentals from garden centers, nurseries, and private and public gardens, as well as imported plant material, were inspected. In total, 577 plant, soil, and potting media samples were tested using various detection methods: lateral flow diagnostic test, enzyme-linked immunosorbent assay, conventional polymerase chain reaction, and isolation, followed by identification based on growth characteristics in culture and morphological features. P. ramorum was not detected in any of the 162 soil or potting media tested by the baiting method. P. ramorum was detected in 12 Rhododendron samples from one private garden in Zemun (City of Belgrade District) exhibiting symptoms of leaf necrosis and blight and petiole necrosis, and in three samples of Pieris spp. from one garden center exhibiting symptoms of leaf necrosis. Eight Phytophthora isolates were obtained from the positive Rhododendron plants and three isolates from Pieris plants, and all were identified as P. ramorum on the basis of their uniform morphological and growth characteristics. P. ramorum conformation was also made by sequencing of the internal transcribed spacer regions for a single isolate taken from one infected rhododendron and one pieris plant. Serbian isolates were determined as A1 mating type, due to formation of a few typical sexual structures when crossed with the A2 mating type of P. cinnamomi and P. cryptogea. Pathogenicity test on non-wounded detached leaves of 19 popular ornamentals, as well as the most frequently imported ones, revealed that 10 host species were susceptible, including Robinia pseudoacacia, which is widely distributed in Serbia. During this study, Cotoneaster horizontalis and C. dammeri were determined to be new experimental hosts of P ramorum. This article provides evidence of P. ramorum introduction into Serbia. Although P. ramorum has not been detected in Serbian production nurseries, its presence outdoors might cause severe damages on susceptible common urban plants in public green and natural ecosystems.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Phytophthora ramorum Occurrence in Ornamentals in Serbia",
pages = "708-703",
number = "6",
volume = "94",
doi = "10.1094/PDIS-94-6-0703"
}

Bulajić, A., Djekić, I., Jović, J., Krnjajić, S., Vučurović, A.,& Krstić, B.. (2010). Phytophthora ramorum Occurrence in Ornamentals in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 94(6), 703-708.
https://doi.org/10.1094/PDIS-94-6-0703

Bulajić A, Djekić I, Jović J, Krnjajić S, Vučurović A, Krstić B. Phytophthora ramorum Occurrence in Ornamentals in Serbia. in Plant Disease. 2010;94(6):703-708.
doi:10.1094/PDIS-94-6-0703 .

Bulajić, Aleksandra, Djekić, Ivana, Jović, Jelena, Krnjajić, Slobodan, Vučurović, Ana, Krstić, Branka, "Phytophthora ramorum Occurrence in Ornamentals in Serbia" in Plant Disease, 94, no. 6 (2010):703-708,
https://doi.org/10.1094/PDIS-94-6-0703 . .

TY  - JOUR
AU  - Bulajić, Aleksandra
AU  - Djekić, Ivana
AU  - Jović, Jelena
AU  - Krnjajić, Slobodan
AU  - Vučurović, Ana
AU  - Krstić, Branka
PY  - 2009
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2047
AB  - In a survey to determine the presence and distribution of It-is yellows, spot virus (IYSV) in greenhouse ornamentals and onion field crops in 14 districts of Serbia as well as on imported ornamental plants, 1,574 samples were collected and analyzed by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). IYSV was not detected in nearly 1,200 plant samples collected from 39 genera of ornamentals grown in greenhouses in Serbia or imported from other countries during 2005 to 2007. The virus was detected in samples from an onion seed crop in the Sirig locality (South Backa District) that showed symptoms resembling those caused by IYSV and in samples without IYSV-Iike symptoms from an onion bulb crop in the Obrenovac locality (City of Belgrade District). Mechanical transmission of IYSV isolates was difficult, and only the isolate 605-SRB could infect four plant species, but not in all replications. No virus transmission could be demonstrated in 5,000 tested seeds originating from IYSV-infected onion crops. For further confirmation of IYSV, the nucleotide sequence of its nucleocapsid (NC) gene was obtained by reverse transcription-polymerase chain reaction (RT-PCR) in symptomatic onion samples as well as in symptomless leaves of Nicotiana benthamiana. Four previously developed primers were tested to determine their suitability for routine detection of Serbian IYSV isolates. Phylogenetic analysis showed clustering of isolates 605-SRB and 622-SRB from the onion seed crop and isolate 283-SRB front the onion bulb crop into two distant clades. The analysis indicated that Serbian isolates of IYSV do not share a recent common ancestor and that they represent two distinct lineages of IYSV in Serbia. Considering that onion is one of the most important and traditionally grown vegetable crops in Serbia, IYSV represents a potentially devastating pathogen in this country.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Incidence and Distribution of Iris yellow spot virus on Onion in Serbia
EP  - 982
IS  - 10
SP  - 976
VL  - 93
DO  - 10.1094/PDIS-93-10-0976
ER  - 

@article{
author = "Bulajić, Aleksandra and Djekić, Ivana and Jović, Jelena and Krnjajić, Slobodan and Vučurović, Ana and Krstić, Branka",
year = "2009",
abstract = "In a survey to determine the presence and distribution of It-is yellows, spot virus (IYSV) in greenhouse ornamentals and onion field crops in 14 districts of Serbia as well as on imported ornamental plants, 1,574 samples were collected and analyzed by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). IYSV was not detected in nearly 1,200 plant samples collected from 39 genera of ornamentals grown in greenhouses in Serbia or imported from other countries during 2005 to 2007. The virus was detected in samples from an onion seed crop in the Sirig locality (South Backa District) that showed symptoms resembling those caused by IYSV and in samples without IYSV-Iike symptoms from an onion bulb crop in the Obrenovac locality (City of Belgrade District). Mechanical transmission of IYSV isolates was difficult, and only the isolate 605-SRB could infect four plant species, but not in all replications. No virus transmission could be demonstrated in 5,000 tested seeds originating from IYSV-infected onion crops. For further confirmation of IYSV, the nucleotide sequence of its nucleocapsid (NC) gene was obtained by reverse transcription-polymerase chain reaction (RT-PCR) in symptomatic onion samples as well as in symptomless leaves of Nicotiana benthamiana. Four previously developed primers were tested to determine their suitability for routine detection of Serbian IYSV isolates. Phylogenetic analysis showed clustering of isolates 605-SRB and 622-SRB from the onion seed crop and isolate 283-SRB front the onion bulb crop into two distant clades. The analysis indicated that Serbian isolates of IYSV do not share a recent common ancestor and that they represent two distinct lineages of IYSV in Serbia. Considering that onion is one of the most important and traditionally grown vegetable crops in Serbia, IYSV represents a potentially devastating pathogen in this country.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Incidence and Distribution of Iris yellow spot virus on Onion in Serbia",
pages = "982-976",
number = "10",
volume = "93",
doi = "10.1094/PDIS-93-10-0976"
}

Bulajić, A., Djekić, I., Jović, J., Krnjajić, S., Vučurović, A.,& Krstić, B.. (2009). Incidence and Distribution of Iris yellow spot virus on Onion in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 93(10), 976-982.
https://doi.org/10.1094/PDIS-93-10-0976

Bulajić A, Djekić I, Jović J, Krnjajić S, Vučurović A, Krstić B. Incidence and Distribution of Iris yellow spot virus on Onion in Serbia. in Plant Disease. 2009;93(10):976-982.
doi:10.1094/PDIS-93-10-0976 .

Bulajić, Aleksandra, Djekić, Ivana, Jović, Jelena, Krnjajić, Slobodan, Vučurović, Ana, Krstić, Branka, "Incidence and Distribution of Iris yellow spot virus on Onion in Serbia" in Plant Disease, 93, no. 10 (2009):976-982,
https://doi.org/10.1094/PDIS-93-10-0976 . .

TY  - JOUR
AU  - Bulajić, Aleksandra
AU  - Jović, Jelena
AU  - Krnjajić, Slobodan
AU  - Djekić, Ilija
AU  - Krstić, Branka
PY  - 2009
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2007
PB  - Wiley-Blackwell, Hoboken
T2  - Plant Pathology
T1  - First report of Phytophthora ramorum on Rhododendron sp in Serbia
EP  - 804
IS  - 4
SP  - 804
VL  - 58
DO  - 10.1111/j.1365-3059.2009.02033.x
ER  - 

@article{
author = "Bulajić, Aleksandra and Jović, Jelena and Krnjajić, Slobodan and Djekić, Ilija and Krstić, Branka",
year = "2009",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Plant Pathology",
title = "First report of Phytophthora ramorum on Rhododendron sp in Serbia",
pages = "804-804",
number = "4",
volume = "58",
doi = "10.1111/j.1365-3059.2009.02033.x"
}

Bulajić, A., Jović, J., Krnjajić, S., Djekić, I.,& Krstić, B.. (2009). First report of Phytophthora ramorum on Rhododendron sp in Serbia. in Plant Pathology
Wiley-Blackwell, Hoboken., 58(4), 804-804.
https://doi.org/10.1111/j.1365-3059.2009.02033.x

Bulajić A, Jović J, Krnjajić S, Djekić I, Krstić B. First report of Phytophthora ramorum on Rhododendron sp in Serbia. in Plant Pathology. 2009;58(4):804-804.
doi:10.1111/j.1365-3059.2009.02033.x .

Bulajić, Aleksandra, Jović, Jelena, Krnjajić, Slobodan, Djekić, Ilija, Krstić, Branka, "First report of Phytophthora ramorum on Rhododendron sp in Serbia" in Plant Pathology, 58, no. 4 (2009):804-804,
https://doi.org/10.1111/j.1365-3059.2009.02033.x . .

TY  - JOUR
AU  - Bulajić, Aleksandra
AU  - Jović, Jelena
AU  - Krnjajić, Slobodan
AU  - Petrov, M.
AU  - Djekić, Ilija
AU  - Krstić, Branka
PY  - 2008
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1814
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First report of Iris yellow spot virus on Onion (Allium cepa) in Serbia
EP  - 1247
IS  - 8
SP  - 1247
VL  - 92
DO  - 10.1094/PDIS-92-8-1247A
ER  - 

@article{
author = "Bulajić, Aleksandra and Jović, Jelena and Krnjajić, Slobodan and Petrov, M. and Djekić, Ilija and Krstić, Branka",
year = "2008",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First report of Iris yellow spot virus on Onion (Allium cepa) in Serbia",
pages = "1247-1247",
number = "8",
volume = "92",
doi = "10.1094/PDIS-92-8-1247A"
}

Bulajić, A., Jović, J., Krnjajić, S., Petrov, M., Djekić, I.,& Krstić, B.. (2008). First report of Iris yellow spot virus on Onion (Allium cepa) in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 92(8), 1247-1247.
https://doi.org/10.1094/PDIS-92-8-1247A

Bulajić A, Jović J, Krnjajić S, Petrov M, Djekić I, Krstić B. First report of Iris yellow spot virus on Onion (Allium cepa) in Serbia. in Plant Disease. 2008;92(8):1247-1247.
doi:10.1094/PDIS-92-8-1247A .

Bulajić, Aleksandra, Jović, Jelena, Krnjajić, Slobodan, Petrov, M., Djekić, Ilija, Krstić, Branka, "First report of Iris yellow spot virus on Onion (Allium cepa) in Serbia" in Plant Disease, 92, no. 8 (2008):1247-1247,
https://doi.org/10.1094/PDIS-92-8-1247A . .

TY  - JOUR
AU  - Djekić, Ivana
AU  - Bulajić, Aleksandra
AU  - Vučurović, Ana
AU  - Krstić, Branka
AU  - Jović, Jelena
AU  - Krnjajić, Slobodan
AU  - Berenji, Janoš
PY  - 2008
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1779
AB  - Three-year investigation of the presence and distribution of tobacco viruses in Serbia showed that Cucumber mosaic virus (CMV) appeared every year with different frequency. During 2007, in two localities CMV was even prevalent both in single and in mixed infections, comparing to all other economically important tobacco viruses. Due to distinct leaf symptoms and stunting of plants, CMV infection causes great crop losses and leaf quality decrease and for that reason CMV appears to be very important tobacco virus in Serbia. Continuous presence of CMV in tobacco crops and its economically destructive effect implied the necessity for the development of a rapid and reliable protocol for CMV molecular detection which would be applicable in diagnostic laboratories in our country. During this investigation a rapid and simple protocol was optimized and developed for molecular detection of CMV in tobacco leaves, using primers CMVAu1u/CMVAu2d and commercially available kits for total RNA extraction as well as for RT-PCR. In RT-PCR by these primers that flank the CMV capsid protein gene, a DNA fragment of 847 bp was amplified in the samples of infected tobacco leaves. Obtained results show that established molecular detection procedure is suitable for reliable and efficient detection of tobacco CMV isolates originating from Serbia. Although serological methods are still useful for large-scale testing of great number of samples, this protocol is, due to its high sensitivity and specificity, an important improvement in conformation of the results obtained by other methods, in the detection of virus low concentrations and in further characterization of CMV isolates originating from Serbia. .
AB  - Trogodišnjim proučavanjima pojave i rasprostranjenosti virusa duvana u Srbiji utvrđeno je da se virus mozaika krastavca (Cucumber mosaic virus, CMV) javlja svake godine sa različitom učestalošću. Tokom 2007. godine na dva lokaliteta gajenja utvrđena je njegova prevalentnost kako u pojedinačnim tako i u mešanim infekcijama u odnosu na sve ostale ekonomski značajne viruse duvana. Usled promena u vidu izraženih simptoma na lišću i kržljavosti biljaka, CMV značajno utiče na smanjenje prinosa i kvaliteta lišća pa se smatra izuzetno važnim virusom duvana za našu zemlju. Stalno prisustvo CMV u usevu duvana i pripadnost grupi ekonomski štetnih virusa uslovili su potrebu za razvijanjem brzog i pouzdanog protokola za molekularnu detekciju koji bi bio primenljiv u dijagnostičkim laboratorijama u našoj zemlji. U toku ovih istraživanja razvijen je i optimiziran brz i precizan protokol za molekularnu detekciju CMV u lišću duvana primenom specifičnih prajmera CMVAu1u/CMVAu2d i komercijalnih kitova za ekstrakciju totalne RNA i RT-PCR. Korišćenjem ovih prajmera, čije se mesto vezivanja na genomu CMV nalazi na granicama gena za proteinski omotač, umnožen je fragment dužine od 847 bp. Dobijeni rezultati ukazuju da se razvijeni postupak za molekularnu identifikaciju može uspešno koristiti za detekciju CMV izolata iz Srbije u duvanu. Serološki metodi i dalje predstavljaju metod izbora za masovna testiranja velikog broja uzoraka, ali razvijeni protokol molekularne identifikacije predstavlja, zbog svoje izuzetne osetljivosti i specifičnosti, sredstvo za potvrdu rezultata dobijenih drugim metodima, kao i metod za dokazivanje virusa u niskoj koncentraciji i način za karakterizaciju CMV izolata poreklom iz Srbije.
PB  - Naučni institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Bilten za hmelj, sirak i lekovito bilje
T1  - Frequency and molecular detection of cucumber mosaic virus in tobacco crops
T1  - Zastupljenost i molekularna detekcija virusa mozaika krastavca u usevu duvana
EP  - 82
IS  - 81
SP  - 70
VL  - 40
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1779
ER  - 

@article{
author = "Djekić, Ivana and Bulajić, Aleksandra and Vučurović, Ana and Krstić, Branka and Jović, Jelena and Krnjajić, Slobodan and Berenji, Janoš",
year = "2008",
abstract = "Three-year investigation of the presence and distribution of tobacco viruses in Serbia showed that Cucumber mosaic virus (CMV) appeared every year with different frequency. During 2007, in two localities CMV was even prevalent both in single and in mixed infections, comparing to all other economically important tobacco viruses. Due to distinct leaf symptoms and stunting of plants, CMV infection causes great crop losses and leaf quality decrease and for that reason CMV appears to be very important tobacco virus in Serbia. Continuous presence of CMV in tobacco crops and its economically destructive effect implied the necessity for the development of a rapid and reliable protocol for CMV molecular detection which would be applicable in diagnostic laboratories in our country. During this investigation a rapid and simple protocol was optimized and developed for molecular detection of CMV in tobacco leaves, using primers CMVAu1u/CMVAu2d and commercially available kits for total RNA extraction as well as for RT-PCR. In RT-PCR by these primers that flank the CMV capsid protein gene, a DNA fragment of 847 bp was amplified in the samples of infected tobacco leaves. Obtained results show that established molecular detection procedure is suitable for reliable and efficient detection of tobacco CMV isolates originating from Serbia. Although serological methods are still useful for large-scale testing of great number of samples, this protocol is, due to its high sensitivity and specificity, an important improvement in conformation of the results obtained by other methods, in the detection of virus low concentrations and in further characterization of CMV isolates originating from Serbia. ., Trogodišnjim proučavanjima pojave i rasprostranjenosti virusa duvana u Srbiji utvrđeno je da se virus mozaika krastavca (Cucumber mosaic virus, CMV) javlja svake godine sa različitom učestalošću. Tokom 2007. godine na dva lokaliteta gajenja utvrđena je njegova prevalentnost kako u pojedinačnim tako i u mešanim infekcijama u odnosu na sve ostale ekonomski značajne viruse duvana. Usled promena u vidu izraženih simptoma na lišću i kržljavosti biljaka, CMV značajno utiče na smanjenje prinosa i kvaliteta lišća pa se smatra izuzetno važnim virusom duvana za našu zemlju. Stalno prisustvo CMV u usevu duvana i pripadnost grupi ekonomski štetnih virusa uslovili su potrebu za razvijanjem brzog i pouzdanog protokola za molekularnu detekciju koji bi bio primenljiv u dijagnostičkim laboratorijama u našoj zemlji. U toku ovih istraživanja razvijen je i optimiziran brz i precizan protokol za molekularnu detekciju CMV u lišću duvana primenom specifičnih prajmera CMVAu1u/CMVAu2d i komercijalnih kitova za ekstrakciju totalne RNA i RT-PCR. Korišćenjem ovih prajmera, čije se mesto vezivanja na genomu CMV nalazi na granicama gena za proteinski omotač, umnožen je fragment dužine od 847 bp. Dobijeni rezultati ukazuju da se razvijeni postupak za molekularnu identifikaciju može uspešno koristiti za detekciju CMV izolata iz Srbije u duvanu. Serološki metodi i dalje predstavljaju metod izbora za masovna testiranja velikog broja uzoraka, ali razvijeni protokol molekularne identifikacije predstavlja, zbog svoje izuzetne osetljivosti i specifičnosti, sredstvo za potvrdu rezultata dobijenih drugim metodima, kao i metod za dokazivanje virusa u niskoj koncentraciji i način za karakterizaciju CMV izolata poreklom iz Srbije.",
publisher = "Naučni institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Bilten za hmelj, sirak i lekovito bilje",
title = "Frequency and molecular detection of cucumber mosaic virus in tobacco crops, Zastupljenost i molekularna detekcija virusa mozaika krastavca u usevu duvana",
pages = "82-70",
number = "81",
volume = "40",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1779"
}

Djekić, I., Bulajić, A., Vučurović, A., Krstić, B., Jović, J., Krnjajić, S.,& Berenji, J.. (2008). Frequency and molecular detection of cucumber mosaic virus in tobacco crops. in Bilten za hmelj, sirak i lekovito bilje
Naučni institut za ratarstvo i povrtarstvo, Novi Sad., 40(81), 70-82.
https://hdl.handle.net/21.15107/rcub_agrospace_1779

Djekić I, Bulajić A, Vučurović A, Krstić B, Jović J, Krnjajić S, Berenji J. Frequency and molecular detection of cucumber mosaic virus in tobacco crops. in Bilten za hmelj, sirak i lekovito bilje. 2008;40(81):70-82.
https://hdl.handle.net/21.15107/rcub_agrospace_1779 .

Djekić, Ivana, Bulajić, Aleksandra, Vučurović, Ana, Krstić, Branka, Jović, Jelena, Krnjajić, Slobodan, Berenji, Janoš, "Frequency and molecular detection of cucumber mosaic virus in tobacco crops" in Bilten za hmelj, sirak i lekovito bilje, 40, no. 81 (2008):70-82,
https://hdl.handle.net/21.15107/rcub_agrospace_1779 .