TY  - JOUR
AU  - Tamindžić, Gordana
AU  - Ignjatov, Maja
AU  - Milošević, Dragana
AU  - Nikolić, Zorica
AU  - Kostić Kravljanac, Ljiljana
AU  - Jovičić, Dušica
AU  - Dolijanović, Željko
AU  - Savić, Jasna
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5931
AB  - Delivery of micronutrients to plants through seed priming improves seedling vigour and increases crops yields. Two-year filed trial was conducted in Pančevo, Serbia, with aim to study the effect of seed priming with zinc (Zn) on field performance of three maize hybrids on calcareous chernozem deficient in plant available Zn. Seed priming treatments were: control (without priming), water priming and priming with 4 mM zinc sulphate water solu-tion. Seed priming had significant effect on early plant growth, plant height, yield components, grain yield and grain Zn concentra-tion. Zn-priming promoted plant growth and increased final plant height. Across two growing seasons with contrasting precipitation and three tested maize hybrids, Zn-priming resulted in an average increase of grain yield by about 18% compared to control, and by about 8.4% compared to water priming. A significant relationship between plant growth parameters, grain yield components and grain yield was detected. Grain Zn concentration was increased by Zn-priming in two hybrids in the season with less precipitation and in one hybrid in the second season. The results imply that using the seeds with elevated Zn content can improve overall field performance of maize grown on calcareous chernozem.
PB  - Page Press Publications
T2  - Italian Journal of Agronomy
T1  - Seed priming with zinc improves field performance of maize hybrids grown on calcareous chernozem
IS  - 3
SP  - 1795
VL  - 16
DO  - 10.4081/ija.2021.1795
ER  - 

@article{
author = "Tamindžić, Gordana and Ignjatov, Maja and Milošević, Dragana and Nikolić, Zorica and Kostić Kravljanac, Ljiljana and Jovičić, Dušica and Dolijanović, Željko and Savić, Jasna",
year = "2021",
abstract = "Delivery of micronutrients to plants through seed priming improves seedling vigour and increases crops yields. Two-year filed trial was conducted in Pančevo, Serbia, with aim to study the effect of seed priming with zinc (Zn) on field performance of three maize hybrids on calcareous chernozem deficient in plant available Zn. Seed priming treatments were: control (without priming), water priming and priming with 4 mM zinc sulphate water solu-tion. Seed priming had significant effect on early plant growth, plant height, yield components, grain yield and grain Zn concentra-tion. Zn-priming promoted plant growth and increased final plant height. Across two growing seasons with contrasting precipitation and three tested maize hybrids, Zn-priming resulted in an average increase of grain yield by about 18% compared to control, and by about 8.4% compared to water priming. A significant relationship between plant growth parameters, grain yield components and grain yield was detected. Grain Zn concentration was increased by Zn-priming in two hybrids in the season with less precipitation and in one hybrid in the second season. The results imply that using the seeds with elevated Zn content can improve overall field performance of maize grown on calcareous chernozem.",
publisher = "Page Press Publications",
journal = "Italian Journal of Agronomy",
title = "Seed priming with zinc improves field performance of maize hybrids grown on calcareous chernozem",
number = "3",
pages = "1795",
volume = "16",
doi = "10.4081/ija.2021.1795"
}

Tamindžić, G., Ignjatov, M., Milošević, D., Nikolić, Z., Kostić Kravljanac, L., Jovičić, D., Dolijanović, Ž.,& Savić, J.. (2021). Seed priming with zinc improves field performance of maize hybrids grown on calcareous chernozem. in Italian Journal of Agronomy
Page Press Publications., 16(3), 1795.
https://doi.org/10.4081/ija.2021.1795

Tamindžić G, Ignjatov M, Milošević D, Nikolić Z, Kostić Kravljanac L, Jovičić D, Dolijanović Ž, Savić J. Seed priming with zinc improves field performance of maize hybrids grown on calcareous chernozem. in Italian Journal of Agronomy. 2021;16(3):1795.
doi:10.4081/ija.2021.1795 .

Tamindžić, Gordana, Ignjatov, Maja, Milošević, Dragana, Nikolić, Zorica, Kostić Kravljanac, Ljiljana, Jovičić, Dušica, Dolijanović, Željko, Savić, Jasna, "Seed priming with zinc improves field performance of maize hybrids grown on calcareous chernozem" in Italian Journal of Agronomy, 16, no. 3 (2021):1795,
https://doi.org/10.4081/ija.2021.1795 . .

TY  - JOUR
AU  - Tamindžić, Gordana
AU  - Ignjatov, Maja
AU  - Milošević, Dragana
AU  - Nikolić, Zorica
AU  - Nastasić, Aleksandra
AU  - Jovičić, Dušica
AU  - Savić, Jasna
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5868
AB  - Good crop establishment is essential for achieving high yield and constraints to good establishment include untimely sowing and low seed quality combined with various adverse growing conditions after sowing. Seed priming is a pre-sowing technique used for the improvement of germination, reduction of the time from sowing to emergence and improvement of emergence uniformity. Various seed priming techniques, such as hydropriming and priming with zinc, are used nowadays to improve crop establishment. The importance of seed priming with zinc for better germination, improved stand establishment, and higher maize yield are well documented. However, there is still a lack of results on the effects of seed priming with water and zinc on seed quality and viability, given that maize seed can be kept in storage for many years without a significant reduction in germination. The study was aimed to evaluate the effects of seed priming with water and Zn on the quality and viability of the maize seed. In order to evaluate the response of four maize hybrids to priming with water (hydropriming) and 4 mM zinc sulphate, primed seeds were subjected to laboratory tests, namely to the germination test, the cold test, and the accelerated aging test. Both priming treatments increased the seed quality, but the beneficial effect of Zn-priming maintained to a larger extent than hydropriming in cold-treated and aged seeds. The negative effects of hydropriming on the viability of the aged seed of hybrid NS 4023 imply a possible limitation to deferred sowing of primed maize seed.
PB  - Institute of Field and Vegetable Crops
T2  - Ratarstvo i Povrtarstvo
T1  - Assessment of quality and viability of primed maize seed [Ocena kvaliteta i životne sposobnosti semena kukuruza nakon prajminga]
EP  - 92
IS  - 3
SP  - 87
VL  - 57
DO  - 10.5937/ratpov57-26575
ER  - 

@article{
author = "Tamindžić, Gordana and Ignjatov, Maja and Milošević, Dragana and Nikolić, Zorica and Nastasić, Aleksandra and Jovičić, Dušica and Savić, Jasna",
year = "2021",
abstract = "Good crop establishment is essential for achieving high yield and constraints to good establishment include untimely sowing and low seed quality combined with various adverse growing conditions after sowing. Seed priming is a pre-sowing technique used for the improvement of germination, reduction of the time from sowing to emergence and improvement of emergence uniformity. Various seed priming techniques, such as hydropriming and priming with zinc, are used nowadays to improve crop establishment. The importance of seed priming with zinc for better germination, improved stand establishment, and higher maize yield are well documented. However, there is still a lack of results on the effects of seed priming with water and zinc on seed quality and viability, given that maize seed can be kept in storage for many years without a significant reduction in germination. The study was aimed to evaluate the effects of seed priming with water and Zn on the quality and viability of the maize seed. In order to evaluate the response of four maize hybrids to priming with water (hydropriming) and 4 mM zinc sulphate, primed seeds were subjected to laboratory tests, namely to the germination test, the cold test, and the accelerated aging test. Both priming treatments increased the seed quality, but the beneficial effect of Zn-priming maintained to a larger extent than hydropriming in cold-treated and aged seeds. The negative effects of hydropriming on the viability of the aged seed of hybrid NS 4023 imply a possible limitation to deferred sowing of primed maize seed.",
publisher = "Institute of Field and Vegetable Crops",
journal = "Ratarstvo i Povrtarstvo",
title = "Assessment of quality and viability of primed maize seed [Ocena kvaliteta i životne sposobnosti semena kukuruza nakon prajminga]",
pages = "92-87",
number = "3",
volume = "57",
doi = "10.5937/ratpov57-26575"
}

Tamindžić, G., Ignjatov, M., Milošević, D., Nikolić, Z., Nastasić, A., Jovičić, D.,& Savić, J.. (2021). Assessment of quality and viability of primed maize seed [Ocena kvaliteta i životne sposobnosti semena kukuruza nakon prajminga]. in Ratarstvo i Povrtarstvo
Institute of Field and Vegetable Crops., 57(3), 87-92.
https://doi.org/10.5937/ratpov57-26575

Tamindžić G, Ignjatov M, Milošević D, Nikolić Z, Nastasić A, Jovičić D, Savić J. Assessment of quality and viability of primed maize seed [Ocena kvaliteta i životne sposobnosti semena kukuruza nakon prajminga]. in Ratarstvo i Povrtarstvo. 2021;57(3):87-92.
doi:10.5937/ratpov57-26575 .

Tamindžić, Gordana, Ignjatov, Maja, Milošević, Dragana, Nikolić, Zorica, Nastasić, Aleksandra, Jovičić, Dušica, Savić, Jasna, "Assessment of quality and viability of primed maize seed [Ocena kvaliteta i životne sposobnosti semena kukuruza nakon prajminga]" in Ratarstvo i Povrtarstvo, 57, no. 3 (2021):87-92,
https://doi.org/10.5937/ratpov57-26575 . .

TY  - JOUR
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Marjanović-Jeromela, Ana
AU  - Nikolić, Zorica
AU  - Tamindžić, Gordana
AU  - Miljaković, Dragana
AU  - Stanković, Ivana
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5867
AB  - Safflower (Carthamus tinctorius L.) is an important oilseed crop belonging to the family Asteraceae. A total of 46 safflower samples were collected from Srbobran locality (South Bačka District) in Serbia in 2015 and analysed for the presence of cucumber mosaic virus (CMV), alfalfa mosaic virus (AMV), and lettuce mosaic virus (LMV), using commercial double-antibody sandwich (DAS)-ELISA kits. Both viruses, CMV and AMV, were detected serologically in the collected samples. None of the analysed samples was found to be positive for LMV. The presence of CMV was further confirmed by mechanical transmission to test the plants including Chenopodium quinoa, C. amaranticolor, Nicotiana glutinosa, and Datura stramonium as well as C. tinctorius, confirming the infectious nature of the disease. Molecular detection of CMV was performed by amplification of a 871 bp fragment in all the tested samples, using the specific primers CMVCPfwd/CMVCPrev that amplify the entire coat protein (CP) gene and part of 3'- and 5'-UTRs of CMV RNA 3. The RT-PCR products derived from the isolates 290Saff and 294Saff were sequenced (MH577791 and MH577792, respectively) and compared with the CMV sequences available in GenBank. Phylogenetic analysis based on CP gene sequences showed clustering of the selected isolates into three subgroups: IA, IB and II. Serbian CMV isolates found in safflower belong to subgroup II. To our knowledge, this is the first report on CMV infection of safflower in Serbia, which has the potential to cause substantial damage to safflower production and pose a threat to other economic crops grown in Serbia.
PB  - Institute of Field and Vegetable Crops
T2  - Ratarstvo i Povrtarstvo
T1  - Presence and molecular characterization of cucumber mosaic virus on safflower in Serbia [Prisustvo i molekularna karakterizacija virusa mozaika krastavca u usevu šafranike u Srbiji]
EP  - 54
IS  - 2
SP  - 49
VL  - 57
DO  - 10.5937/ratpov57-25745
ER  - 

@article{
author = "Milošević, Dragana and Ignjatov, Maja and Marjanović-Jeromela, Ana and Nikolić, Zorica and Tamindžić, Gordana and Miljaković, Dragana and Stanković, Ivana",
year = "2021",
abstract = "Safflower (Carthamus tinctorius L.) is an important oilseed crop belonging to the family Asteraceae. A total of 46 safflower samples were collected from Srbobran locality (South Bačka District) in Serbia in 2015 and analysed for the presence of cucumber mosaic virus (CMV), alfalfa mosaic virus (AMV), and lettuce mosaic virus (LMV), using commercial double-antibody sandwich (DAS)-ELISA kits. Both viruses, CMV and AMV, were detected serologically in the collected samples. None of the analysed samples was found to be positive for LMV. The presence of CMV was further confirmed by mechanical transmission to test the plants including Chenopodium quinoa, C. amaranticolor, Nicotiana glutinosa, and Datura stramonium as well as C. tinctorius, confirming the infectious nature of the disease. Molecular detection of CMV was performed by amplification of a 871 bp fragment in all the tested samples, using the specific primers CMVCPfwd/CMVCPrev that amplify the entire coat protein (CP) gene and part of 3'- and 5'-UTRs of CMV RNA 3. The RT-PCR products derived from the isolates 290Saff and 294Saff were sequenced (MH577791 and MH577792, respectively) and compared with the CMV sequences available in GenBank. Phylogenetic analysis based on CP gene sequences showed clustering of the selected isolates into three subgroups: IA, IB and II. Serbian CMV isolates found in safflower belong to subgroup II. To our knowledge, this is the first report on CMV infection of safflower in Serbia, which has the potential to cause substantial damage to safflower production and pose a threat to other economic crops grown in Serbia.",
publisher = "Institute of Field and Vegetable Crops",
journal = "Ratarstvo i Povrtarstvo",
title = "Presence and molecular characterization of cucumber mosaic virus on safflower in Serbia [Prisustvo i molekularna karakterizacija virusa mozaika krastavca u usevu šafranike u Srbiji]",
pages = "54-49",
number = "2",
volume = "57",
doi = "10.5937/ratpov57-25745"
}

Milošević, D., Ignjatov, M., Marjanović-Jeromela, A., Nikolić, Z., Tamindžić, G., Miljaković, D.,& Stanković, I.. (2021). Presence and molecular characterization of cucumber mosaic virus on safflower in Serbia [Prisustvo i molekularna karakterizacija virusa mozaika krastavca u usevu šafranike u Srbiji]. in Ratarstvo i Povrtarstvo
Institute of Field and Vegetable Crops., 57(2), 49-54.
https://doi.org/10.5937/ratpov57-25745

Milošević D, Ignjatov M, Marjanović-Jeromela A, Nikolić Z, Tamindžić G, Miljaković D, Stanković I. Presence and molecular characterization of cucumber mosaic virus on safflower in Serbia [Prisustvo i molekularna karakterizacija virusa mozaika krastavca u usevu šafranike u Srbiji]. in Ratarstvo i Povrtarstvo. 2021;57(2):49-54.
doi:10.5937/ratpov57-25745 .

Milošević, Dragana, Ignjatov, Maja, Marjanović-Jeromela, Ana, Nikolić, Zorica, Tamindžić, Gordana, Miljaković, Dragana, Stanković, Ivana, "Presence and molecular characterization of cucumber mosaic virus on safflower in Serbia [Prisustvo i molekularna karakterizacija virusa mozaika krastavca u usevu šafranike u Srbiji]" in Ratarstvo i Povrtarstvo, 57, no. 2 (2021):49-54,
https://doi.org/10.5937/ratpov57-25745 . .

TY  - JOUR
AU  - Ivanović, Dragana
AU  - Dodig, Dejan
AU  - Đurić, Nenad
AU  - Kandić, Vesna
AU  - Tamindžić, Gordana
AU  - Nikolić, Nina
AU  - Savić, Jasna
PY  - 2021
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5968
AB  - Wheat grain Zn concentration has important implications for Zn dietary intake in humans. Zn biofortification of wheat by Zn foliar application has been recognized as an efficient tool to enhance grain Zn level. In Serbia, wheat is a staple crop, and no attention is paid to wheat grain as a source of Zn. For the first time, we investigated the effect of Zn foliar application on grain Zn concentration and yield of high-yielding local bread wheat cultivars in Serbia as potential to improve Zn intake in local population. Field experiments were conducted over two growing seasons at two sites located in Vojvodina Province. Zn was applied by spraying of 0.5% water solution of ZnSO4 at the end of flowering stage. Zn foliar application had no significant effect on grain yield. Significant increases of Zn grain concentration resulted from Zn treatment in all of five cultivars tested, ranging from 15 to 49%. No significant difference between the two sites was found in the mean increase in grain Zn concentration by Zn foliar application compared with the control. No correlation was found between grain yield and grain Zn concentration, implying that high grain yields common in intensive wheat production in Serbia do not limit Zn biofortification of grain by Zn foliar application. Zn biofortification of wheat grain by a single foliar spray at the end of the flowering stage can be recommended as an efficient tool to increase Zn intake in human population and prevent low dietary Zn intake.
PB  - Akademiai Kiado ZRt.
T2  - Cereal Research Communications
T1  - Zinc biofortification of bread winter wheat grain by single zinc foliar application
EP  - 679
IS  - 4
SP  - 673
VL  - 49
DO  - 10.1007/s42976-021-00144-2
ER  - 

@article{
author = "Ivanović, Dragana and Dodig, Dejan and Đurić, Nenad and Kandić, Vesna and Tamindžić, Gordana and Nikolić, Nina and Savić, Jasna",
year = "2021",
abstract = "Wheat grain Zn concentration has important implications for Zn dietary intake in humans. Zn biofortification of wheat by Zn foliar application has been recognized as an efficient tool to enhance grain Zn level. In Serbia, wheat is a staple crop, and no attention is paid to wheat grain as a source of Zn. For the first time, we investigated the effect of Zn foliar application on grain Zn concentration and yield of high-yielding local bread wheat cultivars in Serbia as potential to improve Zn intake in local population. Field experiments were conducted over two growing seasons at two sites located in Vojvodina Province. Zn was applied by spraying of 0.5% water solution of ZnSO4 at the end of flowering stage. Zn foliar application had no significant effect on grain yield. Significant increases of Zn grain concentration resulted from Zn treatment in all of five cultivars tested, ranging from 15 to 49%. No significant difference between the two sites was found in the mean increase in grain Zn concentration by Zn foliar application compared with the control. No correlation was found between grain yield and grain Zn concentration, implying that high grain yields common in intensive wheat production in Serbia do not limit Zn biofortification of grain by Zn foliar application. Zn biofortification of wheat grain by a single foliar spray at the end of the flowering stage can be recommended as an efficient tool to increase Zn intake in human population and prevent low dietary Zn intake.",
publisher = "Akademiai Kiado ZRt.",
journal = "Cereal Research Communications",
title = "Zinc biofortification of bread winter wheat grain by single zinc foliar application",
pages = "679-673",
number = "4",
volume = "49",
doi = "10.1007/s42976-021-00144-2"
}

Ivanović, D., Dodig, D., Đurić, N., Kandić, V., Tamindžić, G., Nikolić, N.,& Savić, J.. (2021). Zinc biofortification of bread winter wheat grain by single zinc foliar application. in Cereal Research Communications
Akademiai Kiado ZRt.., 49(4), 673-679.
https://doi.org/10.1007/s42976-021-00144-2

Ivanović D, Dodig D, Đurić N, Kandić V, Tamindžić G, Nikolić N, Savić J. Zinc biofortification of bread winter wheat grain by single zinc foliar application. in Cereal Research Communications. 2021;49(4):673-679.
doi:10.1007/s42976-021-00144-2 .

Ivanović, Dragana, Dodig, Dejan, Đurić, Nenad, Kandić, Vesna, Tamindžić, Gordana, Nikolić, Nina, Savić, Jasna, "Zinc biofortification of bread winter wheat grain by single zinc foliar application" in Cereal Research Communications, 49, no. 4 (2021):673-679,
https://doi.org/10.1007/s42976-021-00144-2 . .

TY  - JOUR
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Marjanović-Jeromela, Ana
AU  - Nikolić, Zorica
AU  - Tamindžić, Gordana
AU  - Miljaković, Dragana
AU  - Stanković, Ivana
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5495
AB  - Safflower (Carthamus tinctorius L.) is an important oilseed crop belonging to the family Asteraceae. A total of 46 safflower samples were collected from Srbobran locality (South Bačka District) in Serbia in 2015 and analysed for the presence of cucumber mosaic virus (CMV), alfalfa mosaic virus (AMV), and lettuce mosaic virus (LMV), using commercial double-antibody sandwich (DAS)-ELISA kits. Both viruses, CMV and AMV, were detected serologically in the collected samples. None of the analysed samples was found to be positive for LMV. The presence of CMV was further confirmed by mechanical transmission to test the plants including Chenopodium quinoa, C. amaranticolor, Nicotiana glutinosa, and Datura stramonium as well as C. tinctorius, confirming the infectious nature of the disease. Molecular detection of CMV was performed by amplification of a 871 bp fragment in all the tested samples, using the specific primers CMVCPfwd/CMVCPrev that amplify the entire coat protein (CP) gene and part of 3'and 5'-UTRs of CMV RNA 3. The RT-PCR products derived from the isolates 290Saff and 294Saff were sequenced (MH577791 and MH577792, respectively) and compared with the CMV sequences available in GenBank. Phylogenetic analysis based on CP gene sequences showed clustering of the selected isolates into three subgroups: IA, IB and II. Serbian CMV isolates found in safflower belong to subgroup II. To our knowledge, this is the first report on CMV infection of safflower in Serbia, which has the potential to cause substantial damage to safflower production and pose a threat to other economic crops grown in Serbia.
AB  - Šafranika (Carthamus tinctorius L.) predstavlja jednu od važnih uljanih kultura koja pripada familiji Asteraceae. Tokom 2015. godine, prikupljeno je 46 uzoraka biljaka šafranike poreklom iz Srbobrana koji su serološki testirani na prisustvo virusa mozaika krastavca (cucumber mosaic virus, CMV), virusa mozaika lucerke (alfalfa mosaic virus, AMV) i virusa mozaika salate (lettuce mosaic virus, LMV) korišćenjem komercijalnih kitova za DAS-ELISA test. U prikupljenim uzorcima dokazano je prisustvo CMV i AMV, dok prisustvo LMV nije dokazano ni u jednom od testiranih uzoraka. Prisustvo CMV je dalje potvrđeno mehaničkim inokulacijama test biljaka Chenopodium quinoa, C. amaranticolor, Nicotianaglutinosa i Datura stramonium kao i na C. tinctorius, čime je potvrđena infektivna priroda oboljenja. Molekularna detekcija CMV obavljena je amplifikacijom fragmenta dužine 871 bp kod svih ispitivanih izolata korišćenjem specifičnih prajmera CMVCPfwd/CMVCPrev koji omogućavaju umnožavanje celog CP gena kao i delove 5' i 3' UTR. U cilju dalje identifikacije, RT-PCR produkti izolata 290Saff i 294Saff su sekvencionisani (MH577791 i MH577792) i upoređeni sa CMV sekvencama dostupnim u GenBank bazi podataka. Filogenetska analiza na osnovu sekvence CP gena pokazala je grupisanje odabranih izolata u tri podgrupe, IA, IB i II. Izolati CMV iz šafranike poreklom iz Srbije grupisali su se u podgrupu II. Prema našim saznanjima, ovo je prvi izveštaj o prisustvu CMV na biljkama šafranike u Srbiji koji može da nanese veliku štetu u proizvodnji ove biljne vrste, a takođe predstavlja pretnju drugim ekonomski značajnim gajenim biljnim vrstama u Srbiji.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Presence and molecular characterization of cucumber mosaic virus on safflower in Serbia
T1  - Prisustvo i molekularna karakterizacija virusa mozaika krastavca u usevu šafranike u Srbiji
EP  - 54
IS  - 2
SP  - 49
VL  - 57
DO  - 10.5937/ratpov57-25745
ER  - 

@article{
author = "Milošević, Dragana and Ignjatov, Maja and Marjanović-Jeromela, Ana and Nikolić, Zorica and Tamindžić, Gordana and Miljaković, Dragana and Stanković, Ivana",
year = "2020",
abstract = "Safflower (Carthamus tinctorius L.) is an important oilseed crop belonging to the family Asteraceae. A total of 46 safflower samples were collected from Srbobran locality (South Bačka District) in Serbia in 2015 and analysed for the presence of cucumber mosaic virus (CMV), alfalfa mosaic virus (AMV), and lettuce mosaic virus (LMV), using commercial double-antibody sandwich (DAS)-ELISA kits. Both viruses, CMV and AMV, were detected serologically in the collected samples. None of the analysed samples was found to be positive for LMV. The presence of CMV was further confirmed by mechanical transmission to test the plants including Chenopodium quinoa, C. amaranticolor, Nicotiana glutinosa, and Datura stramonium as well as C. tinctorius, confirming the infectious nature of the disease. Molecular detection of CMV was performed by amplification of a 871 bp fragment in all the tested samples, using the specific primers CMVCPfwd/CMVCPrev that amplify the entire coat protein (CP) gene and part of 3'and 5'-UTRs of CMV RNA 3. The RT-PCR products derived from the isolates 290Saff and 294Saff were sequenced (MH577791 and MH577792, respectively) and compared with the CMV sequences available in GenBank. Phylogenetic analysis based on CP gene sequences showed clustering of the selected isolates into three subgroups: IA, IB and II. Serbian CMV isolates found in safflower belong to subgroup II. To our knowledge, this is the first report on CMV infection of safflower in Serbia, which has the potential to cause substantial damage to safflower production and pose a threat to other economic crops grown in Serbia., Šafranika (Carthamus tinctorius L.) predstavlja jednu od važnih uljanih kultura koja pripada familiji Asteraceae. Tokom 2015. godine, prikupljeno je 46 uzoraka biljaka šafranike poreklom iz Srbobrana koji su serološki testirani na prisustvo virusa mozaika krastavca (cucumber mosaic virus, CMV), virusa mozaika lucerke (alfalfa mosaic virus, AMV) i virusa mozaika salate (lettuce mosaic virus, LMV) korišćenjem komercijalnih kitova za DAS-ELISA test. U prikupljenim uzorcima dokazano je prisustvo CMV i AMV, dok prisustvo LMV nije dokazano ni u jednom od testiranih uzoraka. Prisustvo CMV je dalje potvrđeno mehaničkim inokulacijama test biljaka Chenopodium quinoa, C. amaranticolor, Nicotianaglutinosa i Datura stramonium kao i na C. tinctorius, čime je potvrđena infektivna priroda oboljenja. Molekularna detekcija CMV obavljena je amplifikacijom fragmenta dužine 871 bp kod svih ispitivanih izolata korišćenjem specifičnih prajmera CMVCPfwd/CMVCPrev koji omogućavaju umnožavanje celog CP gena kao i delove 5' i 3' UTR. U cilju dalje identifikacije, RT-PCR produkti izolata 290Saff i 294Saff su sekvencionisani (MH577791 i MH577792) i upoređeni sa CMV sekvencama dostupnim u GenBank bazi podataka. Filogenetska analiza na osnovu sekvence CP gena pokazala je grupisanje odabranih izolata u tri podgrupe, IA, IB i II. Izolati CMV iz šafranike poreklom iz Srbije grupisali su se u podgrupu II. Prema našim saznanjima, ovo je prvi izveštaj o prisustvu CMV na biljkama šafranike u Srbiji koji može da nanese veliku štetu u proizvodnji ove biljne vrste, a takođe predstavlja pretnju drugim ekonomski značajnim gajenim biljnim vrstama u Srbiji.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Presence and molecular characterization of cucumber mosaic virus on safflower in Serbia, Prisustvo i molekularna karakterizacija virusa mozaika krastavca u usevu šafranike u Srbiji",
pages = "54-49",
number = "2",
volume = "57",
doi = "10.5937/ratpov57-25745"
}

Milošević, D., Ignjatov, M., Marjanović-Jeromela, A., Nikolić, Z., Tamindžić, G., Miljaković, D.,& Stanković, I.. (2020). Presence and molecular characterization of cucumber mosaic virus on safflower in Serbia. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 57(2), 49-54.
https://doi.org/10.5937/ratpov57-25745

Milošević D, Ignjatov M, Marjanović-Jeromela A, Nikolić Z, Tamindžić G, Miljaković D, Stanković I. Presence and molecular characterization of cucumber mosaic virus on safflower in Serbia. in Ratarstvo i povrtarstvo. 2020;57(2):49-54.
doi:10.5937/ratpov57-25745 .

Milošević, Dragana, Ignjatov, Maja, Marjanović-Jeromela, Ana, Nikolić, Zorica, Tamindžić, Gordana, Miljaković, Dragana, Stanković, Ivana, "Presence and molecular characterization of cucumber mosaic virus on safflower in Serbia" in Ratarstvo i povrtarstvo, 57, no. 2 (2020):49-54,
https://doi.org/10.5937/ratpov57-25745 . .

TY  - JOUR
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Marjanović-Jeromela, Ana
AU  - Stanković, Ivana
AU  - Nikolić, Zorica
AU  - Tamindžić, Gordana
AU  - Krstić, Branka
PY  - 2019
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5234
AB  - In 2018, a total of 58 mustard samples from two different localities in Serbia (Rimski Šančevi and Senta) were collected and tested for the presence of Turnip yellow virus (TuYV), Cauliflower mosaic virus (CaMV) and Turnip mosaic virus (TuMV) by DAS-ELTSA. TuYV was serologically detected in 42 tested samples while all collected samples were negative for CaMV and TuMV. By using aphid transmission tests, five test plants were inoculated with two ELTSApositive TuYV samples of naturally infected mustard plants. Virus species identification was performed by amplification of a 780 bp fragment in all tested samples using the specific primers TuYVorf0F/TuYVorf0R. The RTPCR products from two isolates 88Sal (MK144816) and 98Bni (MK144817) were sequenced and compared with the GenBank sequences of TuYV. Serbian isolates showed the highest identity with Poland TuYV isolate (EU022489). Phylogenetic analysis showed that TuYV isolates from Serbia were clustered with other TuYV sequences retrieved from the GenBank.
AB  - Tokom 2018. godine, sa dva lokaliteta gajenja slačice, ukupno je sakupljeno 58 uzoraka koji su serološki testirani na prisustvo Turnip yellow virus (TuYV), Cauliflower mosaic virus (CaMV) i Turnip mosaic virus (TuMV) korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo TuYV serološki je dokazano u 42 sakupljena uzorka slačice, dok prisustvo CaMV i TuMV nije dokazano ni u jednom od testiranih uzoraka. Za dalja istraživanja odabrana su dva izolata TuYV prirodno zaraženih biljaka slačice, koji su uspešno preneti vašima na pet različitih test biljaka, čime je potvrđena infektivna priroda oboljenja. Molekularna detekcija obavljena je amplifikacijom fragmenta dužine 780 bp kod četiri ispitivana izolata korišćenjem specifičnih prajmera TuYVorf0F i TuYVorfOR. RT-PCR produkti izolata 88Sal i 98Bni su sekvencirani (MK144816 i MK144817) i upoređeni sa TuYV sekvencama dostupnim u GenBank bazi podataka. Izolati iz Srbije su pokazali najviši stepen nukleotidne sličnosti od 98,9% i 99% (100% aminokiselinska sličnost) sa izolatom TuYV Br iz Poljske (EU022489). Filogenetska analiza pokazala je grupisanje TuYV izolata iz Srbije zajedno sa ostalim TuYV izolatima iz GenBank baze podataka.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Molecular characterization of Turnip yellows virus: A new pathogen of mustards in Serbia
T1  - Molekularna karakterizacija Turnip yellows virus - novog patogena slačice u Srbiji
EP  - 87
IS  - 3
SP  - 82
VL  - 56
DO  - 10.5937/ratpov56-23514
ER  - 

@article{
author = "Milošević, Dragana and Ignjatov, Maja and Marjanović-Jeromela, Ana and Stanković, Ivana and Nikolić, Zorica and Tamindžić, Gordana and Krstić, Branka",
year = "2019",
abstract = "In 2018, a total of 58 mustard samples from two different localities in Serbia (Rimski Šančevi and Senta) were collected and tested for the presence of Turnip yellow virus (TuYV), Cauliflower mosaic virus (CaMV) and Turnip mosaic virus (TuMV) by DAS-ELTSA. TuYV was serologically detected in 42 tested samples while all collected samples were negative for CaMV and TuMV. By using aphid transmission tests, five test plants were inoculated with two ELTSApositive TuYV samples of naturally infected mustard plants. Virus species identification was performed by amplification of a 780 bp fragment in all tested samples using the specific primers TuYVorf0F/TuYVorf0R. The RTPCR products from two isolates 88Sal (MK144816) and 98Bni (MK144817) were sequenced and compared with the GenBank sequences of TuYV. Serbian isolates showed the highest identity with Poland TuYV isolate (EU022489). Phylogenetic analysis showed that TuYV isolates from Serbia were clustered with other TuYV sequences retrieved from the GenBank., Tokom 2018. godine, sa dva lokaliteta gajenja slačice, ukupno je sakupljeno 58 uzoraka koji su serološki testirani na prisustvo Turnip yellow virus (TuYV), Cauliflower mosaic virus (CaMV) i Turnip mosaic virus (TuMV) korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo TuYV serološki je dokazano u 42 sakupljena uzorka slačice, dok prisustvo CaMV i TuMV nije dokazano ni u jednom od testiranih uzoraka. Za dalja istraživanja odabrana su dva izolata TuYV prirodno zaraženih biljaka slačice, koji su uspešno preneti vašima na pet različitih test biljaka, čime je potvrđena infektivna priroda oboljenja. Molekularna detekcija obavljena je amplifikacijom fragmenta dužine 780 bp kod četiri ispitivana izolata korišćenjem specifičnih prajmera TuYVorf0F i TuYVorfOR. RT-PCR produkti izolata 88Sal i 98Bni su sekvencirani (MK144816 i MK144817) i upoređeni sa TuYV sekvencama dostupnim u GenBank bazi podataka. Izolati iz Srbije su pokazali najviši stepen nukleotidne sličnosti od 98,9% i 99% (100% aminokiselinska sličnost) sa izolatom TuYV Br iz Poljske (EU022489). Filogenetska analiza pokazala je grupisanje TuYV izolata iz Srbije zajedno sa ostalim TuYV izolatima iz GenBank baze podataka.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Molecular characterization of Turnip yellows virus: A new pathogen of mustards in Serbia, Molekularna karakterizacija Turnip yellows virus - novog patogena slačice u Srbiji",
pages = "87-82",
number = "3",
volume = "56",
doi = "10.5937/ratpov56-23514"
}

Milošević, D., Ignjatov, M., Marjanović-Jeromela, A., Stanković, I., Nikolić, Z., Tamindžić, G.,& Krstić, B.. (2019). Molecular characterization of Turnip yellows virus: A new pathogen of mustards in Serbia. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 56(3), 82-87.
https://doi.org/10.5937/ratpov56-23514

Milošević D, Ignjatov M, Marjanović-Jeromela A, Stanković I, Nikolić Z, Tamindžić G, Krstić B. Molecular characterization of Turnip yellows virus: A new pathogen of mustards in Serbia. in Ratarstvo i povrtarstvo. 2019;56(3):82-87.
doi:10.5937/ratpov56-23514 .

Milošević, Dragana, Ignjatov, Maja, Marjanović-Jeromela, Ana, Stanković, Ivana, Nikolić, Zorica, Tamindžić, Gordana, Krstić, Branka, "Molecular characterization of Turnip yellows virus: A new pathogen of mustards in Serbia" in Ratarstvo i povrtarstvo, 56, no. 3 (2019):82-87,
https://doi.org/10.5937/ratpov56-23514 . .

TY  - JOUR
AU  - Tamindžić, Gordana
AU  - Nikolić, Zorica T.
AU  - Savić, Jasna
AU  - Milošević, Dragana
AU  - Petrović, Gordana R.
AU  - Ivanović, Dragana
AU  - Ignjatov, Maja
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4219
AB  - Seed treatment is a common way of fungicide and insecticide use nowadays, since this way of pesticide application can provide the best protection in the vicinity of the future plant. The aim of research was to evaluate the effects of different seed treatments on germination and seedling growth in three maize inbred lines. The research included the seed treatment with several combinations of a fungicide Maxim XL 035-FS (a.i. metalaxil-M + fludioxonil) and neonicotinoid insecticides Gaucho 600-FS (a.i. imidacloprid) and Cruiser 350-FS (a.i. thiamethoxam), as well as untreated seed (control). The results indicated that inbred lines 21202 x 21101 NS and 317659 NS had a highly vigourous seed which was not affected by the seed treatments. The seed treatments with Maxim XL 035- FS+Gaucho 600-FS and Maxim XL 035-FS+Cruiser 350-FS led to a decrease in germination (90.25% and 89.50%, respectively) of maize inbred line 306081 NS as well as a decrease in root length (126.75 mm and 125.25 mm, respectively) and dry root weight (0.135 g and 0.1875 g, respectively) of maize inbred line 21202 x 21101 NS. All seed treatments had positive effects on root growth, as well as on fresh root weight of maize inbred lines 306081 NS and 317659 NS.
AB  - Tretiranje semena fungicidima i insekticidima je uobičajen način pripreme semena pre setve, s obzirom na to da ovaj način primene pesticida može da pruži najbolju zaštitu mlade biljke od patogena i štetočina u zemljištu. Cilj ovog istraživanja bio je da se ispita uticaj različitih tretmana semena pesticidima na klijanje semena i početni porast izdanaka različitih inbred linija kukuruza. Istraživanje je obuhvatilo testiranje tretmana semena inbred linija kukuruza sa nekoliko kombinacija fungicida (Maxim XL 035-FS, fludioksonil, 25 g/l + metalaksil-M, 10 g/l) i insekticida (Gaucho 600-FS, imidakloprid 600 g/l i Cruiser 350-FS, tiametoksam, 350 g/l). Netretirano seme je uzeto za kontrolu. Rezultati istraživanja su pokazali da inbred linije 21202 x 21101 NS i 317659 NS imaju visoko vigorozno seme, na koje nisu uticali testirani preparati. Testirane kombinacije preparata pesticida Maxim XL 035-FS+Gaucho 600-FS i Maxim XL 035-FS+Cruiser 350-FS dovele su do smanjenja klijavosti semena inbred linije 306081 NS (90,25%, odnosno 89,50%), kao i do smanjenja dužine korena (126,75 mm, odnosno 125,25 mm) i mase suvog korena (0,135 g, odnosno 0,1875 g) izdanaka inbred linije 21202 x 21101 NS. Svi testirani tretmani imali su pozitivan uticaj na porast korena izdanaka, kao i na masu svežeg korena izdanaka inbred linija 306081 NS i 317659 NS.
PB  - Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd
T2  - Journal of Agricultural Sciences (Belgrade)
T1  - Seedling growth of maize (Zea mays L.) inbred lines affected by seed treatment with pesticides
T1  - Porast izdanaka inbred linija kukuruza (Zea mays L.) pod uticajem tretmana semena pesticidima
EP  - 235
IS  - 3
SP  - 227
VL  - 61
DO  - 10.2298/JAS1603227T
ER  - 

@article{
author = "Tamindžić, Gordana and Nikolić, Zorica T. and Savić, Jasna and Milošević, Dragana and Petrović, Gordana R. and Ivanović, Dragana and Ignjatov, Maja",
year = "2016",
abstract = "Seed treatment is a common way of fungicide and insecticide use nowadays, since this way of pesticide application can provide the best protection in the vicinity of the future plant. The aim of research was to evaluate the effects of different seed treatments on germination and seedling growth in three maize inbred lines. The research included the seed treatment with several combinations of a fungicide Maxim XL 035-FS (a.i. metalaxil-M + fludioxonil) and neonicotinoid insecticides Gaucho 600-FS (a.i. imidacloprid) and Cruiser 350-FS (a.i. thiamethoxam), as well as untreated seed (control). The results indicated that inbred lines 21202 x 21101 NS and 317659 NS had a highly vigourous seed which was not affected by the seed treatments. The seed treatments with Maxim XL 035- FS+Gaucho 600-FS and Maxim XL 035-FS+Cruiser 350-FS led to a decrease in germination (90.25% and 89.50%, respectively) of maize inbred line 306081 NS as well as a decrease in root length (126.75 mm and 125.25 mm, respectively) and dry root weight (0.135 g and 0.1875 g, respectively) of maize inbred line 21202 x 21101 NS. All seed treatments had positive effects on root growth, as well as on fresh root weight of maize inbred lines 306081 NS and 317659 NS., Tretiranje semena fungicidima i insekticidima je uobičajen način pripreme semena pre setve, s obzirom na to da ovaj način primene pesticida može da pruži najbolju zaštitu mlade biljke od patogena i štetočina u zemljištu. Cilj ovog istraživanja bio je da se ispita uticaj različitih tretmana semena pesticidima na klijanje semena i početni porast izdanaka različitih inbred linija kukuruza. Istraživanje je obuhvatilo testiranje tretmana semena inbred linija kukuruza sa nekoliko kombinacija fungicida (Maxim XL 035-FS, fludioksonil, 25 g/l + metalaksil-M, 10 g/l) i insekticida (Gaucho 600-FS, imidakloprid 600 g/l i Cruiser 350-FS, tiametoksam, 350 g/l). Netretirano seme je uzeto za kontrolu. Rezultati istraživanja su pokazali da inbred linije 21202 x 21101 NS i 317659 NS imaju visoko vigorozno seme, na koje nisu uticali testirani preparati. Testirane kombinacije preparata pesticida Maxim XL 035-FS+Gaucho 600-FS i Maxim XL 035-FS+Cruiser 350-FS dovele su do smanjenja klijavosti semena inbred linije 306081 NS (90,25%, odnosno 89,50%), kao i do smanjenja dužine korena (126,75 mm, odnosno 125,25 mm) i mase suvog korena (0,135 g, odnosno 0,1875 g) izdanaka inbred linije 21202 x 21101 NS. Svi testirani tretmani imali su pozitivan uticaj na porast korena izdanaka, kao i na masu svežeg korena izdanaka inbred linija 306081 NS i 317659 NS.",
publisher = "Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd",
journal = "Journal of Agricultural Sciences (Belgrade)",
title = "Seedling growth of maize (Zea mays L.) inbred lines affected by seed treatment with pesticides, Porast izdanaka inbred linija kukuruza (Zea mays L.) pod uticajem tretmana semena pesticidima",
pages = "235-227",
number = "3",
volume = "61",
doi = "10.2298/JAS1603227T"
}

Tamindžić, G., Nikolić, Z. T., Savić, J., Milošević, D., Petrović, G. R., Ivanović, D.,& Ignjatov, M.. (2016). Seedling growth of maize (Zea mays L.) inbred lines affected by seed treatment with pesticides. in Journal of Agricultural Sciences (Belgrade)
Univerzitet u Beogradu - Poljoprivredni fakultet, Beograd., 61(3), 227-235.
https://doi.org/10.2298/JAS1603227T

Tamindžić G, Nikolić ZT, Savić J, Milošević D, Petrović GR, Ivanović D, Ignjatov M. Seedling growth of maize (Zea mays L.) inbred lines affected by seed treatment with pesticides. in Journal of Agricultural Sciences (Belgrade). 2016;61(3):227-235.
doi:10.2298/JAS1603227T .

Tamindžić, Gordana, Nikolić, Zorica T., Savić, Jasna, Milošević, Dragana, Petrović, Gordana R., Ivanović, Dragana, Ignjatov, Maja, "Seedling growth of maize (Zea mays L.) inbred lines affected by seed treatment with pesticides" in Journal of Agricultural Sciences (Belgrade), 61, no. 3 (2016):227-235,
https://doi.org/10.2298/JAS1603227T . .

TY  - JOUR
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Nikolić, Zorica
AU  - Gvozdanović-Varga, Jelica
AU  - Tamindžić, Gordana
AU  - Stanković, Ivana
AU  - Krstić, Branka
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3947
AB  - During 2014 a total of 67 pot marigold samples from five different localities in the Province in Vojvodina were collected and analysed for the presence of Cucumber mosaic virus (CMV) and Impatiens necrotic spot virus (INSV) using commercial double-antibody sandwich (DAS)-ELISA kits. CMV was detected serologically in all inspected localities in 67.16% collected samples. None of the analysed samples was positive for INSV. The virus was successfully mechanically transmitted to test plants including Chenopodium amaranticolor, C. quinoa, Datura stramonium, Nicotiana tabacum 'Samsun' and N. glutinosa, as well as pot marigold seedlings, confirming the infectious nature of the disease. The presence of CMV in pot marigold plants was further verified by RT-PCR and sequencing, using the specific primers CMV CPfwd/CMVCPrev that amplify coat protein (CP) gene. Phylogenetic analysis based on the CP gene sequences showed clustering of the selected isolates into three subgroups, IA, IB and II, and Serbian CMV isolates from pot marigold belong to subgroup II.
AB  - Tokom 2014. godine, sa pet različitih lokaliteta gajenja nevena u Vojvodini sakupljeno je ukupno 67 uzorka koji su serološki testirani na prisustvo virusa mozaika krastavca (Cucumber mosaic virus, CMV) i virusa nekrotične pegavosti impatiensa (Impatiens necrotic spot virus, INSV), korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo CMV dokazano je na svih pet pregledanih lokaliteta i to u 67,16% prikupljenih uzoraka, dok prisustvo INSV nije dokazano ni u jednom od testiranih uzoraka. Virus je uspešno prenet mehaničkim inokulacijama test biljaka Chenopodium amaranticolor, C. quinoa, Nicotiana tabacum 'Samsun' i N. glutinosa, kao i na sejance nevena, čime je potvrđena infektivna priroda oboljenja. Prisustvo CMV u biljkama nevena potvrđeno je primenom RT-PCR metode i sekvencioniranjem uz korišćenje specifičnih prajmera CMVCPfwd/CMVCPrev koji omogućavaju umnožavanje gena za protein omotača (CP gena). Filogenetska analiza na osnovu sekvence CP gena pokazala je grupisanje izolata u tri podgrupe, IA, IB i II, a izolati CMV iz nevena iz Srbije grupišu se u podgrupu II.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - The presence of cucumber mosaic virus in pot marigold (Calendula officinalis L.) in Serbia
T1  - Prisustvo virusa mozaika krastavca na nevenu (Calendula officinalis L.) u Srbiji
EP  - 73
IS  - 2
SP  - 67
VL  - 52
DO  - 10.5937/ratpov52-7814
ER  - 

@article{
author = "Milošević, Dragana and Ignjatov, Maja and Nikolić, Zorica and Gvozdanović-Varga, Jelica and Tamindžić, Gordana and Stanković, Ivana and Krstić, Branka",
year = "2015",
abstract = "During 2014 a total of 67 pot marigold samples from five different localities in the Province in Vojvodina were collected and analysed for the presence of Cucumber mosaic virus (CMV) and Impatiens necrotic spot virus (INSV) using commercial double-antibody sandwich (DAS)-ELISA kits. CMV was detected serologically in all inspected localities in 67.16% collected samples. None of the analysed samples was positive for INSV. The virus was successfully mechanically transmitted to test plants including Chenopodium amaranticolor, C. quinoa, Datura stramonium, Nicotiana tabacum 'Samsun' and N. glutinosa, as well as pot marigold seedlings, confirming the infectious nature of the disease. The presence of CMV in pot marigold plants was further verified by RT-PCR and sequencing, using the specific primers CMV CPfwd/CMVCPrev that amplify coat protein (CP) gene. Phylogenetic analysis based on the CP gene sequences showed clustering of the selected isolates into three subgroups, IA, IB and II, and Serbian CMV isolates from pot marigold belong to subgroup II., Tokom 2014. godine, sa pet različitih lokaliteta gajenja nevena u Vojvodini sakupljeno je ukupno 67 uzorka koji su serološki testirani na prisustvo virusa mozaika krastavca (Cucumber mosaic virus, CMV) i virusa nekrotične pegavosti impatiensa (Impatiens necrotic spot virus, INSV), korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo CMV dokazano je na svih pet pregledanih lokaliteta i to u 67,16% prikupljenih uzoraka, dok prisustvo INSV nije dokazano ni u jednom od testiranih uzoraka. Virus je uspešno prenet mehaničkim inokulacijama test biljaka Chenopodium amaranticolor, C. quinoa, Nicotiana tabacum 'Samsun' i N. glutinosa, kao i na sejance nevena, čime je potvrđena infektivna priroda oboljenja. Prisustvo CMV u biljkama nevena potvrđeno je primenom RT-PCR metode i sekvencioniranjem uz korišćenje specifičnih prajmera CMVCPfwd/CMVCPrev koji omogućavaju umnožavanje gena za protein omotača (CP gena). Filogenetska analiza na osnovu sekvence CP gena pokazala je grupisanje izolata u tri podgrupe, IA, IB i II, a izolati CMV iz nevena iz Srbije grupišu se u podgrupu II.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "The presence of cucumber mosaic virus in pot marigold (Calendula officinalis L.) in Serbia, Prisustvo virusa mozaika krastavca na nevenu (Calendula officinalis L.) u Srbiji",
pages = "73-67",
number = "2",
volume = "52",
doi = "10.5937/ratpov52-7814"
}

Milošević, D., Ignjatov, M., Nikolić, Z., Gvozdanović-Varga, J., Tamindžić, G., Stanković, I.,& Krstić, B.. (2015). The presence of cucumber mosaic virus in pot marigold (Calendula officinalis L.) in Serbia. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 52(2), 67-73.
https://doi.org/10.5937/ratpov52-7814

Milošević D, Ignjatov M, Nikolić Z, Gvozdanović-Varga J, Tamindžić G, Stanković I, Krstić B. The presence of cucumber mosaic virus in pot marigold (Calendula officinalis L.) in Serbia. in Ratarstvo i povrtarstvo. 2015;52(2):67-73.
doi:10.5937/ratpov52-7814 .

Milošević, Dragana, Ignjatov, Maja, Nikolić, Zorica, Gvozdanović-Varga, Jelica, Tamindžić, Gordana, Stanković, Ivana, Krstić, Branka, "The presence of cucumber mosaic virus in pot marigold (Calendula officinalis L.) in Serbia" in Ratarstvo i povrtarstvo, 52, no. 2 (2015):67-73,
https://doi.org/10.5937/ratpov52-7814 . .