TY  - JOUR
AU  - Popović, Vera
AU  - Sikora, Vladimir
AU  - Berenji, Janoš
AU  - Filipović, Vladimir
AU  - Dolijanović, Željko
AU  - Ikanović, Jela
AU  - Dončić, Dalibor
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3587
AB  - During the period 2010-2011 about 2.113 million ha of buckwheat was sown annually worldwide. Average yield of buckwheat during the monitored period was 913 kg ha-1. Areas and average yield have a rising tendency. The most significant producers of buckwheat in the world are: China, Russia and Ukraine. In Serbia buckwheat is produced on small areas. The paper presents results of testing of four buckwheat varieties, produced on plots of the Institute for crops and vegetables as follows: Novosadska, Godijevo, Bamby and Češka. Analysis of average yield has shown that Novosadska variety produced statistically significant higher yield (2626 kg ha-1) compared to the other varieties tested (p  lt 0.05). From the results shown we can see that buckwheat yield in Serbia is significantly higher compared with the world average yield, which tells us that this plant can be successfully produced in our agro-ecological conditions of growing.
AB  - U svetu je, u periodu 2010-2011., pod usevom heljde bilo zasejano godišnje u proseku oko 2,113 miliona ha. Prosečni prinosi heljde u posmatranom periodu iznosili su 913 kg ha-1. Površine i prinosi beleže trend rasta po stopi od 22,46 % i 17,12% godišnje. Najznačajniji proizvođači heljde u svetu su: Kina, Rusija i Ukrajina. U Srbiji se heljda proizvodi na malim površinama. U radu su prikazani i rezultati ispitivanja četiri sorte heljde, proizvedene na parcelama Instituta za ratarstvo i povrtarstvo: Novosadska, Godijevo, Bamby i Češka. Analiza prosečnih prinosa pokazala je da je sorta Novosadska ostvarila statistički značajno viši prinos (2626 kg ha-1) u odnosu na ostale ispitivane sorte (p  lt 0,05). Iz prikazanih rezultata vidimo da su prinosi heljde u Srbiji značajno viši u odnosu na prosečne svetske prinose što nam govori da se ova gajena biljka može uspešno proizvoditi i u našim agroekološkim uslovima gajenja.
PB  - Naučno društvo agrarnih ekonomista Balkana, Beograd, Institut za ekonomiku poljoprivrede, Beograd i Akademija ekonomskih nauka, Bukurešt
T2  - Ekonomika poljoprivrede
T1  - Analysis of buckwheat production in the world and Serbia
T1  - Analiza proizvodnje heljde u svetu i u Srbiji
EP  - 62
IS  - 1
SP  - 53
VL  - 61
DO  - 10.5937/ekoPolj1401053P
ER  - 

@article{
author = "Popović, Vera and Sikora, Vladimir and Berenji, Janoš and Filipović, Vladimir and Dolijanović, Željko and Ikanović, Jela and Dončić, Dalibor",
year = "2014",
abstract = "During the period 2010-2011 about 2.113 million ha of buckwheat was sown annually worldwide. Average yield of buckwheat during the monitored period was 913 kg ha-1. Areas and average yield have a rising tendency. The most significant producers of buckwheat in the world are: China, Russia and Ukraine. In Serbia buckwheat is produced on small areas. The paper presents results of testing of four buckwheat varieties, produced on plots of the Institute for crops and vegetables as follows: Novosadska, Godijevo, Bamby and Češka. Analysis of average yield has shown that Novosadska variety produced statistically significant higher yield (2626 kg ha-1) compared to the other varieties tested (p  lt 0.05). From the results shown we can see that buckwheat yield in Serbia is significantly higher compared with the world average yield, which tells us that this plant can be successfully produced in our agro-ecological conditions of growing., U svetu je, u periodu 2010-2011., pod usevom heljde bilo zasejano godišnje u proseku oko 2,113 miliona ha. Prosečni prinosi heljde u posmatranom periodu iznosili su 913 kg ha-1. Površine i prinosi beleže trend rasta po stopi od 22,46 % i 17,12% godišnje. Najznačajniji proizvođači heljde u svetu su: Kina, Rusija i Ukrajina. U Srbiji se heljda proizvodi na malim površinama. U radu su prikazani i rezultati ispitivanja četiri sorte heljde, proizvedene na parcelama Instituta za ratarstvo i povrtarstvo: Novosadska, Godijevo, Bamby i Češka. Analiza prosečnih prinosa pokazala je da je sorta Novosadska ostvarila statistički značajno viši prinos (2626 kg ha-1) u odnosu na ostale ispitivane sorte (p  lt 0,05). Iz prikazanih rezultata vidimo da su prinosi heljde u Srbiji značajno viši u odnosu na prosečne svetske prinose što nam govori da se ova gajena biljka može uspešno proizvoditi i u našim agroekološkim uslovima gajenja.",
publisher = "Naučno društvo agrarnih ekonomista Balkana, Beograd, Institut za ekonomiku poljoprivrede, Beograd i Akademija ekonomskih nauka, Bukurešt",
journal = "Ekonomika poljoprivrede",
title = "Analysis of buckwheat production in the world and Serbia, Analiza proizvodnje heljde u svetu i u Srbiji",
pages = "62-53",
number = "1",
volume = "61",
doi = "10.5937/ekoPolj1401053P"
}

Popović, V., Sikora, V., Berenji, J., Filipović, V., Dolijanović, Ž., Ikanović, J.,& Dončić, D.. (2014). Analysis of buckwheat production in the world and Serbia. in Ekonomika poljoprivrede
Naučno društvo agrarnih ekonomista Balkana, Beograd, Institut za ekonomiku poljoprivrede, Beograd i Akademija ekonomskih nauka, Bukurešt., 61(1), 53-62.
https://doi.org/10.5937/ekoPolj1401053P

Popović V, Sikora V, Berenji J, Filipović V, Dolijanović Ž, Ikanović J, Dončić D. Analysis of buckwheat production in the world and Serbia. in Ekonomika poljoprivrede. 2014;61(1):53-62.
doi:10.5937/ekoPolj1401053P .

Popović, Vera, Sikora, Vladimir, Berenji, Janoš, Filipović, Vladimir, Dolijanović, Željko, Ikanović, Jela, Dončić, Dalibor, "Analysis of buckwheat production in the world and Serbia" in Ekonomika poljoprivrede, 61, no. 1 (2014):53-62,
https://doi.org/10.5937/ekoPolj1401053P . .

TY  - JOUR
AU  - Rabrenović, Biljana
AU  - Dimić, Etelka
AU  - Berenji, Janoš
AU  - Vujasinović, Vesna B.
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2939
AB  - The technico-technological and chemical characteristics of seed of 8 free-fertilized and hybrid sorts of oil pumpkin seed with and without hull and two commercial samples of oil pumpkin were investigated. Liter mass and mass of 1000 grains were determined and the percentage of hull in seed samples with hull. The chemical composition was determined investigating the content of moisture, oil, proteins, mineral matter, fiber and total carbohydrates. Regarding the technico-technological characteristics, no significant differences between seeds of free-fertilized and F1 hybrids of oil pumpkih hull-less seed and seed with hull were found. The self-fertilized sorts and F1 hybrids of oil pumpkin, both hull-less and seed with hull, are characterized by high oil content, in the range of 43,27 to 54,78%, calculated on dry matter. The protein content was also high, from 34,97 to 36,61%, on dry matter.
AB  - Ispitivane su tehničko-tehnološke i hemijske karakteristike semena 8 slobodnooplodnih i hibridnih sorti uljane tikve golice i sa ljuskom i dva komercijalna uzorka uljanih tikvi. Od tehničko-tehnoloških karakteristika semena određene su litarska masa, masa 1000 semena i određen je procenat ljuske kod uzoraka semena sa ljuskom. Hemijski sastav semena određen je ispitivanjem sadržaja vlage, ulja, proteina, mineralnih materija, celuloze i ukupnih ugljenih hidrata. U pogledu tehničko-tehnoloških karakteristika nisu postojale značajne razlike između semena slobodnooplodnih i F1 hibrida uljanih tikvi golica i sa ljuskom, kao ni komercijalnih uzoraka, što ukazuje da poreklo semena nije imalo uticaja na ove karakteristike. Samooplodne sorte i F1 hibridi uljanih tikvi sa semenom golica i semenom sa ljuskom, odlikovale su se visokim sadržajem ulja koji se kretao od 43,27do 54,78% računato na suvu materiju, a karakterističan je bio i visok sadržaj proteina koji se kretao od 34,97 do 36,61%, računato na suvu materiju.
PB  - Univerzitet u Novom Sadu - Tehnološki fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Uljarstvo
T1  - Technological characteristics and quality of seed of freefertilized nd hybrid oil pumpkin sorts
T1  - Tehnološke karakteristike i kvalitet semena slobodnooplodnih i hibridnih sorti uljane tikve
EP  - 31
IS  - 1-2
SP  - 25
VL  - 43
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_2939
ER  - 

@article{
author = "Rabrenović, Biljana and Dimić, Etelka and Berenji, Janoš and Vujasinović, Vesna B.",
year = "2012",
abstract = "The technico-technological and chemical characteristics of seed of 8 free-fertilized and hybrid sorts of oil pumpkin seed with and without hull and two commercial samples of oil pumpkin were investigated. Liter mass and mass of 1000 grains were determined and the percentage of hull in seed samples with hull. The chemical composition was determined investigating the content of moisture, oil, proteins, mineral matter, fiber and total carbohydrates. Regarding the technico-technological characteristics, no significant differences between seeds of free-fertilized and F1 hybrids of oil pumpkih hull-less seed and seed with hull were found. The self-fertilized sorts and F1 hybrids of oil pumpkin, both hull-less and seed with hull, are characterized by high oil content, in the range of 43,27 to 54,78%, calculated on dry matter. The protein content was also high, from 34,97 to 36,61%, on dry matter., Ispitivane su tehničko-tehnološke i hemijske karakteristike semena 8 slobodnooplodnih i hibridnih sorti uljane tikve golice i sa ljuskom i dva komercijalna uzorka uljanih tikvi. Od tehničko-tehnoloških karakteristika semena određene su litarska masa, masa 1000 semena i određen je procenat ljuske kod uzoraka semena sa ljuskom. Hemijski sastav semena određen je ispitivanjem sadržaja vlage, ulja, proteina, mineralnih materija, celuloze i ukupnih ugljenih hidrata. U pogledu tehničko-tehnoloških karakteristika nisu postojale značajne razlike između semena slobodnooplodnih i F1 hibrida uljanih tikvi golica i sa ljuskom, kao ni komercijalnih uzoraka, što ukazuje da poreklo semena nije imalo uticaja na ove karakteristike. Samooplodne sorte i F1 hibridi uljanih tikvi sa semenom golica i semenom sa ljuskom, odlikovale su se visokim sadržajem ulja koji se kretao od 43,27do 54,78% računato na suvu materiju, a karakterističan je bio i visok sadržaj proteina koji se kretao od 34,97 do 36,61%, računato na suvu materiju.",
publisher = "Univerzitet u Novom Sadu - Tehnološki fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Uljarstvo",
title = "Technological characteristics and quality of seed of freefertilized nd hybrid oil pumpkin sorts, Tehnološke karakteristike i kvalitet semena slobodnooplodnih i hibridnih sorti uljane tikve",
pages = "31-25",
number = "1-2",
volume = "43",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_2939"
}

Rabrenović, B., Dimić, E., Berenji, J.,& Vujasinović, V. B.. (2012). Technological characteristics and quality of seed of freefertilized nd hybrid oil pumpkin sorts. in Uljarstvo
Univerzitet u Novom Sadu - Tehnološki fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad., 43(1-2), 25-31.
https://hdl.handle.net/21.15107/rcub_agrospace_2939

Rabrenović B, Dimić E, Berenji J, Vujasinović VB. Technological characteristics and quality of seed of freefertilized nd hybrid oil pumpkin sorts. in Uljarstvo. 2012;43(1-2):25-31.
https://hdl.handle.net/21.15107/rcub_agrospace_2939 .

Rabrenović, Biljana, Dimić, Etelka, Berenji, Janoš, Vujasinović, Vesna B., "Technological characteristics and quality of seed of freefertilized nd hybrid oil pumpkin sorts" in Uljarstvo, 43, no. 1-2 (2012):25-31,
https://hdl.handle.net/21.15107/rcub_agrospace_2939 .

TY  - JOUR
AU  - Vučurović, Ana
AU  - Bulajić, Aleksandra
AU  - Stanković, Ivana
AU  - Ristić, Danijela
AU  - Nikolić, D.
AU  - Berenji, Janoš
AU  - Krstić, Branka
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3000
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Zucchini yellow mosaic virus in Watermelon in Serbia
EP  - 149
IS  - 1
SP  - 149
VL  - 96
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_3000
ER  - 

@article{
author = "Vučurović, Ana and Bulajić, Aleksandra and Stanković, Ivana and Ristić, Danijela and Nikolić, D. and Berenji, Janoš and Krstić, Branka",
year = "2012",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Zucchini yellow mosaic virus in Watermelon in Serbia",
pages = "149-149",
number = "1",
volume = "96",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_3000"
}

Vučurović, A., Bulajić, A., Stanković, I., Ristić, D., Nikolić, D., Berenji, J.,& Krstić, B.. (2012). First Report of Zucchini yellow mosaic virus in Watermelon in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 96(1), 149-149.
https://hdl.handle.net/21.15107/rcub_agrospace_3000

Vučurović A, Bulajić A, Stanković I, Ristić D, Nikolić D, Berenji J, Krstić B. First Report of Zucchini yellow mosaic virus in Watermelon in Serbia. in Plant Disease. 2012;96(1):149-149.
https://hdl.handle.net/21.15107/rcub_agrospace_3000 .

Vučurović, Ana, Bulajić, Aleksandra, Stanković, Ivana, Ristić, Danijela, Nikolić, D., Berenji, Janoš, Krstić, Branka, "First Report of Zucchini yellow mosaic virus in Watermelon in Serbia" in Plant Disease, 96, no. 1 (2012):149-149,
https://hdl.handle.net/21.15107/rcub_agrospace_3000 .

TY  - JOUR
AU  - Vučurović, Ana
AU  - Bulajić, Aleksandra
AU  - Stanković, Ivana
AU  - Ristić, Danijela
AU  - Berenji, Janoš
AU  - Jović, Jelena
AU  - Krstić, Branka
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2961
AB  - Cucurbit species grown in the Vojvodina Province, Serbia, were surveyed for the incidence of Zucchini yellow mosaic virus (ZYMV), Watermelon mosaic virus (WMV), Cucumber mosaic virus (CMV), Squash mosaic virus (SqMV), Papaya ringspot virus (PRSV) and Tobacco ringspot virus (TRSV) from 2007 to 2009. Samples from more than 700 pumpkin, squash and bottle gourd plants with virus-like symptoms were analyzed by double-antibody sandwich (DAS)-ELISA. ZYMV, WMV and CMV were detected in 79.2, 32.2, and 12.8% of tested samples, respectively. WMV was prevalent in 2007 and ZYMV in 2008-09. Mixed infections were the most frequent type in 2007-08 in contrast to 2009 when single infection of ZYMV prevailed. ZYMV was the most widespread being found in 33 out of 39 inspected fields. Virus species identification was confirmed in selected samples by conventional reverse transcription-polymerase chain reaction (RT-PCR) and sequencing of their coat protein genes. By comparing the obtained virus isolate sequences with those available in GenBank, the identification of serologically detected viruses was confirmed. Phylogenetic analysis based on complete coat protein (CP) sequences highlighted that Serbian ZYMV isolates were closely related to other Central European ZYMV isolates. Finally, additional testing of ELISA-negative samples by RT-PCR using primers specific to six other mosaic viruses revealed the presence of Tomato spotted wilt virus (TSWV) in winter (Cucurbita maxima) and summer (C. pepo 'Beogradska') squash. This is the first report of TSWV natural occurrence on cucurbits in Serbia and on winter squash worldwide.
PB  - Springer, Dordrecht
T2  - European Journal of Plant Pathology
T1  - Non-persistently aphid-borne viruses infecting pumpkin and squash in Serbia and partial characterization of Zucchini yellow mosaic virus isolates
EP  - 947
IS  - 4
SP  - 935
VL  - 133
DO  - 10.1007/s10658-012-9964-x
ER  - 

@article{
author = "Vučurović, Ana and Bulajić, Aleksandra and Stanković, Ivana and Ristić, Danijela and Berenji, Janoš and Jović, Jelena and Krstić, Branka",
year = "2012",
abstract = "Cucurbit species grown in the Vojvodina Province, Serbia, were surveyed for the incidence of Zucchini yellow mosaic virus (ZYMV), Watermelon mosaic virus (WMV), Cucumber mosaic virus (CMV), Squash mosaic virus (SqMV), Papaya ringspot virus (PRSV) and Tobacco ringspot virus (TRSV) from 2007 to 2009. Samples from more than 700 pumpkin, squash and bottle gourd plants with virus-like symptoms were analyzed by double-antibody sandwich (DAS)-ELISA. ZYMV, WMV and CMV were detected in 79.2, 32.2, and 12.8% of tested samples, respectively. WMV was prevalent in 2007 and ZYMV in 2008-09. Mixed infections were the most frequent type in 2007-08 in contrast to 2009 when single infection of ZYMV prevailed. ZYMV was the most widespread being found in 33 out of 39 inspected fields. Virus species identification was confirmed in selected samples by conventional reverse transcription-polymerase chain reaction (RT-PCR) and sequencing of their coat protein genes. By comparing the obtained virus isolate sequences with those available in GenBank, the identification of serologically detected viruses was confirmed. Phylogenetic analysis based on complete coat protein (CP) sequences highlighted that Serbian ZYMV isolates were closely related to other Central European ZYMV isolates. Finally, additional testing of ELISA-negative samples by RT-PCR using primers specific to six other mosaic viruses revealed the presence of Tomato spotted wilt virus (TSWV) in winter (Cucurbita maxima) and summer (C. pepo 'Beogradska') squash. This is the first report of TSWV natural occurrence on cucurbits in Serbia and on winter squash worldwide.",
publisher = "Springer, Dordrecht",
journal = "European Journal of Plant Pathology",
title = "Non-persistently aphid-borne viruses infecting pumpkin and squash in Serbia and partial characterization of Zucchini yellow mosaic virus isolates",
pages = "947-935",
number = "4",
volume = "133",
doi = "10.1007/s10658-012-9964-x"
}

Vučurović, A., Bulajić, A., Stanković, I., Ristić, D., Berenji, J., Jović, J.,& Krstić, B.. (2012). Non-persistently aphid-borne viruses infecting pumpkin and squash in Serbia and partial characterization of Zucchini yellow mosaic virus isolates. in European Journal of Plant Pathology
Springer, Dordrecht., 133(4), 935-947.
https://doi.org/10.1007/s10658-012-9964-x

Vučurović A, Bulajić A, Stanković I, Ristić D, Berenji J, Jović J, Krstić B. Non-persistently aphid-borne viruses infecting pumpkin and squash in Serbia and partial characterization of Zucchini yellow mosaic virus isolates. in European Journal of Plant Pathology. 2012;133(4):935-947.
doi:10.1007/s10658-012-9964-x .

Vučurović, Ana, Bulajić, Aleksandra, Stanković, Ivana, Ristić, Danijela, Berenji, Janoš, Jović, Jelena, Krstić, Branka, "Non-persistently aphid-borne viruses infecting pumpkin and squash in Serbia and partial characterization of Zucchini yellow mosaic virus isolates" in European Journal of Plant Pathology, 133, no. 4 (2012):935-947,
https://doi.org/10.1007/s10658-012-9964-x . .

TY  - JOUR
AU  - Ristić, Danijela
AU  - Stanković, Ivana
AU  - Vučurović, Ana
AU  - Berenji, Janoš
AU  - Krnjajić, Slobodan
AU  - Krstić, Branka
AU  - Bulajić, Aleksandra
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2936
AB  - Sixteen samples of sorghum seed (Sorghum bicolor (L.) Moench.) 'Alba', 'Gold', 'Prima' and 'Reform' were analyzed in the localities of Bački Petrovac and Čantavir in the period 2009-2011. Tipresence of species belonging to the genera Epicoccum, Fusarium, Alternaria, Aspergillus and Penicillium was established in single and mixed infections. From the infected sorghum seed, monosporial cultures identified as Epicoccum nigrum based on morphology, proved their pathogenicity on artificially inoculated sorghum seedlings. Molecular identification was performed by PCR and amplification of the ITS region of ribosomal DNA. Gene sequences of selected isolates 291-09 (JQ619838) and 315-09 (JQ619839) exhibited 99-100% nucleotide identity with the sequences of 31 isolates of E. nigrum deposited in the GenBank. It obtained results represent the first detailed characterization of E. nigrum in Serbia. The presence of a large number of phytopathogenic fungi on sorghum seed should be further investigated in order to clarify their relationships and relative significance.
AB  - U periodu 2009-2011. godine na lokalitetima Bački Petrovac i Čantavir prikupljeno je i analizirano 16 uzoraka zaraženog semena gajenog sirka (Sorghum bicolor (L.) Moench.) sorti Alba, Gold, Prima i Reform na prisustvo fitopatogenih gljiva. U pojedinačnim i mešanim zarazama ustanovljeno je prisustvo vrsta iz rodova Epicoccum, Fusarium, Alternaria, Aspergillus i Penicillium. Iz zaraženog semena izolovane su monosporne kulture i na osnovu morfoloških osobina identifikovane su kao Epicoccum nigrum. Patogenost izolata ove gljive potvrđena je pojavom simptoma na veštački inokulisanim sejancima sirka. Molekularna identifikacija obavljena je primenom PCR i amplifikacije ITS regiona ribozomalne DNK. Sekvence gena odabranih izolata 291-09 (JQ619838) i 315-09 (JQ619839) pokazale su 99-100% nukleotidne identičnosti sa sekvencama 31 izolata E. nigrum deponovanihu GenBank bazi podataka. Dobijeni rezultati predstavljaju prvu detaljnu karakterizaciju E. nigrum u Srbiji. Prisustvo većeg broja vrsta gljiva na semenu sirka zahteva dalja ispitivanja njihovih međusobnih odnosa i značaja.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Epicoccum nigrum the new pathogen of sorghum seed in Serbia
T1  - Epicoccum nigrum novi patogen semena sirka u Srbiji
EP  - 166
IS  - 2
SP  - 160
VL  - 49
DO  - 10.5937/ratpov49-1793
ER  - 

@article{
author = "Ristić, Danijela and Stanković, Ivana and Vučurović, Ana and Berenji, Janoš and Krnjajić, Slobodan and Krstić, Branka and Bulajić, Aleksandra",
year = "2012",
abstract = "Sixteen samples of sorghum seed (Sorghum bicolor (L.) Moench.) 'Alba', 'Gold', 'Prima' and 'Reform' were analyzed in the localities of Bački Petrovac and Čantavir in the period 2009-2011. Tipresence of species belonging to the genera Epicoccum, Fusarium, Alternaria, Aspergillus and Penicillium was established in single and mixed infections. From the infected sorghum seed, monosporial cultures identified as Epicoccum nigrum based on morphology, proved their pathogenicity on artificially inoculated sorghum seedlings. Molecular identification was performed by PCR and amplification of the ITS region of ribosomal DNA. Gene sequences of selected isolates 291-09 (JQ619838) and 315-09 (JQ619839) exhibited 99-100% nucleotide identity with the sequences of 31 isolates of E. nigrum deposited in the GenBank. It obtained results represent the first detailed characterization of E. nigrum in Serbia. The presence of a large number of phytopathogenic fungi on sorghum seed should be further investigated in order to clarify their relationships and relative significance., U periodu 2009-2011. godine na lokalitetima Bački Petrovac i Čantavir prikupljeno je i analizirano 16 uzoraka zaraženog semena gajenog sirka (Sorghum bicolor (L.) Moench.) sorti Alba, Gold, Prima i Reform na prisustvo fitopatogenih gljiva. U pojedinačnim i mešanim zarazama ustanovljeno je prisustvo vrsta iz rodova Epicoccum, Fusarium, Alternaria, Aspergillus i Penicillium. Iz zaraženog semena izolovane su monosporne kulture i na osnovu morfoloških osobina identifikovane su kao Epicoccum nigrum. Patogenost izolata ove gljive potvrđena je pojavom simptoma na veštački inokulisanim sejancima sirka. Molekularna identifikacija obavljena je primenom PCR i amplifikacije ITS regiona ribozomalne DNK. Sekvence gena odabranih izolata 291-09 (JQ619838) i 315-09 (JQ619839) pokazale su 99-100% nukleotidne identičnosti sa sekvencama 31 izolata E. nigrum deponovanihu GenBank bazi podataka. Dobijeni rezultati predstavljaju prvu detaljnu karakterizaciju E. nigrum u Srbiji. Prisustvo većeg broja vrsta gljiva na semenu sirka zahteva dalja ispitivanja njihovih međusobnih odnosa i značaja.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Epicoccum nigrum the new pathogen of sorghum seed in Serbia, Epicoccum nigrum novi patogen semena sirka u Srbiji",
pages = "166-160",
number = "2",
volume = "49",
doi = "10.5937/ratpov49-1793"
}

Ristić, D., Stanković, I., Vučurović, A., Berenji, J., Krnjajić, S., Krstić, B.,& Bulajić, A.. (2012). Epicoccum nigrum the new pathogen of sorghum seed in Serbia. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 49(2), 160-166.
https://doi.org/10.5937/ratpov49-1793

Ristić D, Stanković I, Vučurović A, Berenji J, Krnjajić S, Krstić B, Bulajić A. Epicoccum nigrum the new pathogen of sorghum seed in Serbia. in Ratarstvo i povrtarstvo. 2012;49(2):160-166.
doi:10.5937/ratpov49-1793 .

Ristić, Danijela, Stanković, Ivana, Vučurović, Ana, Berenji, Janoš, Krnjajić, Slobodan, Krstić, Branka, Bulajić, Aleksandra, "Epicoccum nigrum the new pathogen of sorghum seed in Serbia" in Ratarstvo i povrtarstvo, 49, no. 2 (2012):160-166,
https://doi.org/10.5937/ratpov49-1793 . .

TY  - JOUR
AU  - Vučurović, Ana
AU  - Bulajić, Aleksandra
AU  - Milojević, Katarina
AU  - Stanković, Ivana
AU  - Ristić, Danijela
AU  - Berenji, Janoš
AU  - Krstić, Branka
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2935
AB  - The presence of Zucchini yellow mosaic virus (ZYMV) in two out of seven watermelon production localities in Serbia during 2011 was investigated by analyzing leaves sampled from symptomatic and asymptomatic watermelon plants and utilizing DAS-ELISA test. In the locality of Gornji Tavankut, ZYMV was detected in 23.08% of tested plants in single infections, and in the locality of Silbas it was detected in 35.29% of tested plants in mixed infections with Cucumber mosaic virus and Alfalfa mosaic virus. ZYMV was successfully mechanically transmitted from naturally infected watermelon plants to Cucurbita pepo 'Ezra F1'. Molecular detection was performed by RT-PCR and amplification of part of the gene for nuclear inclusions, gene of coat protein and part of 3' non-coding region, which confirmed the identification of the ZYMV isolates. Phylogenetic analysis revealed grouping of the isolate originating from watermelon with other isolates from Serbia and Central Europe within A-I subgroup. Analysis of amino acid sequences of the N terminal end of the CP gene revealed that isolate 550-11 belongs to the Central European branch.
AB  - Prisustvo virusa žutog mozaika cukinija (Zucchini yellow mosaic virus, ZYMV) na dva od sedam lokaliteta gajenja lubenice u Srbiji tokom 2011. godine utvrđeno je analizom uzoraka lišća simptomatičnih i asimptomatičnih biljaka lubenice primenom DAS-ELISA metode. Na lokalitetu Gornji Tavankut, ZYMV je dokazan u 23,08% testiranih biljaka u pojedinačnim infekcijama, a na lokalitetu Silbaš u 35,29% testiranih biljaka u mešanim infekcijama sa virusom mozaika krastavca i virusom mozaika lubenice. ZYMV je uspešno mehanički prenesen sa prirodno zaraženih biljaka lubenice na Cucurbita pepo 'Ezra F1'. Molekularna detekcija obavljena je RT-PCR metodom umnožavanjem dela gena za nuklearne inkluzije, gena za protein omotača i dela 3' neprepisujućeg regiona, čime je potvrđena identifikacija izolata. Filogenetske analize pokazale su grupisanje izolata poreklom iz lubenice sa drugim izolatima iz Srbije i Centralne Evrope u okviru A-I podgrupe. Analize aminokiselinske sekvence N-terminalnog kraja CP gena, takođe su pokazale pripadnost izolata 550-11 centralnoevropskoj grani.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Presence and characterization of Zucchini yellow mosaic virus in watermelon in Serbia
T1  - Prisustvo i karakterizacija virusa žutog mozaika cukinija u usevu lubenice u Srbiji
EP  - 159
IS  - 2
SP  - 151
VL  - 49
DO  - 10.5937/ratpov49-1773
ER  - 

@article{
author = "Vučurović, Ana and Bulajić, Aleksandra and Milojević, Katarina and Stanković, Ivana and Ristić, Danijela and Berenji, Janoš and Krstić, Branka",
year = "2012",
abstract = "The presence of Zucchini yellow mosaic virus (ZYMV) in two out of seven watermelon production localities in Serbia during 2011 was investigated by analyzing leaves sampled from symptomatic and asymptomatic watermelon plants and utilizing DAS-ELISA test. In the locality of Gornji Tavankut, ZYMV was detected in 23.08% of tested plants in single infections, and in the locality of Silbas it was detected in 35.29% of tested plants in mixed infections with Cucumber mosaic virus and Alfalfa mosaic virus. ZYMV was successfully mechanically transmitted from naturally infected watermelon plants to Cucurbita pepo 'Ezra F1'. Molecular detection was performed by RT-PCR and amplification of part of the gene for nuclear inclusions, gene of coat protein and part of 3' non-coding region, which confirmed the identification of the ZYMV isolates. Phylogenetic analysis revealed grouping of the isolate originating from watermelon with other isolates from Serbia and Central Europe within A-I subgroup. Analysis of amino acid sequences of the N terminal end of the CP gene revealed that isolate 550-11 belongs to the Central European branch., Prisustvo virusa žutog mozaika cukinija (Zucchini yellow mosaic virus, ZYMV) na dva od sedam lokaliteta gajenja lubenice u Srbiji tokom 2011. godine utvrđeno je analizom uzoraka lišća simptomatičnih i asimptomatičnih biljaka lubenice primenom DAS-ELISA metode. Na lokalitetu Gornji Tavankut, ZYMV je dokazan u 23,08% testiranih biljaka u pojedinačnim infekcijama, a na lokalitetu Silbaš u 35,29% testiranih biljaka u mešanim infekcijama sa virusom mozaika krastavca i virusom mozaika lubenice. ZYMV je uspešno mehanički prenesen sa prirodno zaraženih biljaka lubenice na Cucurbita pepo 'Ezra F1'. Molekularna detekcija obavljena je RT-PCR metodom umnožavanjem dela gena za nuklearne inkluzije, gena za protein omotača i dela 3' neprepisujućeg regiona, čime je potvrđena identifikacija izolata. Filogenetske analize pokazale su grupisanje izolata poreklom iz lubenice sa drugim izolatima iz Srbije i Centralne Evrope u okviru A-I podgrupe. Analize aminokiselinske sekvence N-terminalnog kraja CP gena, takođe su pokazale pripadnost izolata 550-11 centralnoevropskoj grani.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Presence and characterization of Zucchini yellow mosaic virus in watermelon in Serbia, Prisustvo i karakterizacija virusa žutog mozaika cukinija u usevu lubenice u Srbiji",
pages = "159-151",
number = "2",
volume = "49",
doi = "10.5937/ratpov49-1773"
}

Vučurović, A., Bulajić, A., Milojević, K., Stanković, I., Ristić, D., Berenji, J.,& Krstić, B.. (2012). Presence and characterization of Zucchini yellow mosaic virus in watermelon in Serbia. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 49(2), 151-159.
https://doi.org/10.5937/ratpov49-1773

Vučurović A, Bulajić A, Milojević K, Stanković I, Ristić D, Berenji J, Krstić B. Presence and characterization of Zucchini yellow mosaic virus in watermelon in Serbia. in Ratarstvo i povrtarstvo. 2012;49(2):151-159.
doi:10.5937/ratpov49-1773 .

Vučurović, Ana, Bulajić, Aleksandra, Milojević, Katarina, Stanković, Ivana, Ristić, Danijela, Berenji, Janoš, Krstić, Branka, "Presence and characterization of Zucchini yellow mosaic virus in watermelon in Serbia" in Ratarstvo i povrtarstvo, 49, no. 2 (2012):151-159,
https://doi.org/10.5937/ratpov49-1773 . .

TY  - JOUR
AU  - Vučurović, Ana
AU  - Bulajić, Aleksandra
AU  - Stanković, Ivana
AU  - Ristić, Danijela
AU  - Berenji, Janoš
AU  - Jović, Jelena
AU  - Krstić, Branka
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2663
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of the Occurrence of Cucurbit aphid-borne yellows virus on Oilseed Pumpkin in Serbia
EP  - 1035
IS  - 8
SP  - 1035
VL  - 95
DO  - 10.1094/PDIS-02-11-0147
ER  - 

@article{
author = "Vučurović, Ana and Bulajić, Aleksandra and Stanković, Ivana and Ristić, Danijela and Berenji, Janoš and Jović, Jelena and Krstić, Branka",
year = "2011",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of the Occurrence of Cucurbit aphid-borne yellows virus on Oilseed Pumpkin in Serbia",
pages = "1035-1035",
number = "8",
volume = "95",
doi = "10.1094/PDIS-02-11-0147"
}

Vučurović, A., Bulajić, A., Stanković, I., Ristić, D., Berenji, J., Jović, J.,& Krstić, B.. (2011). First Report of the Occurrence of Cucurbit aphid-borne yellows virus on Oilseed Pumpkin in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 95(8), 1035-1035.
https://doi.org/10.1094/PDIS-02-11-0147

Vučurović A, Bulajić A, Stanković I, Ristić D, Berenji J, Jović J, Krstić B. First Report of the Occurrence of Cucurbit aphid-borne yellows virus on Oilseed Pumpkin in Serbia. in Plant Disease. 2011;95(8):1035-1035.
doi:10.1094/PDIS-02-11-0147 .

Vučurović, Ana, Bulajić, Aleksandra, Stanković, Ivana, Ristić, Danijela, Berenji, Janoš, Jović, Jelena, Krstić, Branka, "First Report of the Occurrence of Cucurbit aphid-borne yellows virus on Oilseed Pumpkin in Serbia" in Plant Disease, 95, no. 8 (2011):1035-1035,
https://doi.org/10.1094/PDIS-02-11-0147 . .

TY  - JOUR
AU  - Stanković, Ivana
AU  - Bulajić, Aleksandra
AU  - Vučurović, Ana
AU  - Ristić, Danijela
AU  - Milojević, Katarina
AU  - Berenji, Janoš
AU  - Krstić, Branka
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2614
AB  - In a four-year survey to determine the presence and distribution of viruses in tobacco crops at 17 localities of the Vojvodina Province and Central Serbia, 380 samples were collected and analyzed by DAS-ELISA. Out of the seven viruses tested, tomato spotted wilt virus (TSWV), potato virus Y (PVY), tobacco mosaic virus (TMV), cucumber mosaic virus (CMV), and alfalfa mosaic virus (AMV) were detected in 37.9, 33.4, 28.7, 23.9, and 15.5% of the total tested samples, respectively. TSWV was the most frequently found virus at the localities of Central Serbia, while PVY and CMV were the most frequent viruses in the Vojvodina Province. Single infections were prevalent in years 2005-2007 and the most frequent were those of PVY A triple combination of those viruses was most frequent mixed infection type in 2008. The presence of all five detected viruses was confirmed in selected ELISA-positive samples by RT-PCR and sequencing. The comparisons of obtained virus isolate sequences with those available in NCBI, confirmed the authenticity of serologically detected viruses. Phylogenetic analysis based on partial nucleocapsid gene sequences revealed a joint clustering of Serbian, Bulgarian and Montenegrin TSWV isolates into one geographic subpopulation, which was distinct from the other subpopulation of TSWV isolates from the rest of the European countries. The high incidence of viruses in Serbian tobacco crops highlights the importance of enhancing farmers knowledge towards better implementation of control strategies for preventing serious losses.
PB  - Aepress Sro, Bratislava
T2  - Acta Virologica
T1  - Status of tobacco viruses in Serbia and molecular characterization of tomato spotted wilt virus isolates
EP  - 347
IS  - 4
SP  - 337
VL  - 55
DO  - 10.4149/av_2011_04_337
ER  - 

@article{
author = "Stanković, Ivana and Bulajić, Aleksandra and Vučurović, Ana and Ristić, Danijela and Milojević, Katarina and Berenji, Janoš and Krstić, Branka",
year = "2011",
abstract = "In a four-year survey to determine the presence and distribution of viruses in tobacco crops at 17 localities of the Vojvodina Province and Central Serbia, 380 samples were collected and analyzed by DAS-ELISA. Out of the seven viruses tested, tomato spotted wilt virus (TSWV), potato virus Y (PVY), tobacco mosaic virus (TMV), cucumber mosaic virus (CMV), and alfalfa mosaic virus (AMV) were detected in 37.9, 33.4, 28.7, 23.9, and 15.5% of the total tested samples, respectively. TSWV was the most frequently found virus at the localities of Central Serbia, while PVY and CMV were the most frequent viruses in the Vojvodina Province. Single infections were prevalent in years 2005-2007 and the most frequent were those of PVY A triple combination of those viruses was most frequent mixed infection type in 2008. The presence of all five detected viruses was confirmed in selected ELISA-positive samples by RT-PCR and sequencing. The comparisons of obtained virus isolate sequences with those available in NCBI, confirmed the authenticity of serologically detected viruses. Phylogenetic analysis based on partial nucleocapsid gene sequences revealed a joint clustering of Serbian, Bulgarian and Montenegrin TSWV isolates into one geographic subpopulation, which was distinct from the other subpopulation of TSWV isolates from the rest of the European countries. The high incidence of viruses in Serbian tobacco crops highlights the importance of enhancing farmers knowledge towards better implementation of control strategies for preventing serious losses.",
publisher = "Aepress Sro, Bratislava",
journal = "Acta Virologica",
title = "Status of tobacco viruses in Serbia and molecular characterization of tomato spotted wilt virus isolates",
pages = "347-337",
number = "4",
volume = "55",
doi = "10.4149/av_2011_04_337"
}

Stanković, I., Bulajić, A., Vučurović, A., Ristić, D., Milojević, K., Berenji, J.,& Krstić, B.. (2011). Status of tobacco viruses in Serbia and molecular characterization of tomato spotted wilt virus isolates. in Acta Virologica
Aepress Sro, Bratislava., 55(4), 337-347.
https://doi.org/10.4149/av_2011_04_337

Stanković I, Bulajić A, Vučurović A, Ristić D, Milojević K, Berenji J, Krstić B. Status of tobacco viruses in Serbia and molecular characterization of tomato spotted wilt virus isolates. in Acta Virologica. 2011;55(4):337-347.
doi:10.4149/av_2011_04_337 .

Stanković, Ivana, Bulajić, Aleksandra, Vučurović, Ana, Ristić, Danijela, Milojević, Katarina, Berenji, Janoš, Krstić, Branka, "Status of tobacco viruses in Serbia and molecular characterization of tomato spotted wilt virus isolates" in Acta Virologica, 55, no. 4 (2011):337-347,
https://doi.org/10.4149/av_2011_04_337 . .

TY  - JOUR
AU  - Ristić, Danijela
AU  - Vučurović, Ana
AU  - Stanković, Ivana
AU  - Nikolić, Dušan
AU  - Berenji, Janoš
AU  - Krstić, Branka
AU  - Bulajić, Aleksandra
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2608
AB  - A total of 39 samples of sorghum (Sorghum bicolor) with symptoms of stem and root rot were collected and analyzed during 2009-2011 in Bački Petrovac and Čantavir, Serbia. Monosporic cultures were isolated from stem tissue, their pathogenicity was confirmed by the development of symptoms on artificially inoculated sorghum plants, and they were identified on the basis of macroscopic and microscopic morphological features as Fusarium graminearum. Molecular identification was performed utilizing polimerase chain reaction (PCR) with primer pair ef1/ ef2 and by amplification of protein coding TEF 1-alpha gen. Sequence of TEF gene from the selected isolate 535- 10 (JF747146) showed 98-99% nucleotide identity with sequences of 63 Gibberella zeae isolates deposited in NCBI GenBank. Amplification of the barcoding region of F. graminearum genome of sorghum isolate, contributes to the fast and accurate identification and characterization of Fusarium species in Serbia.
AB  - U periodu 2009-2011. na lokalitetima u Bačkom Petrovcu i Čantaviru prikupljeno je i analizirano 39 uzoraka biljaka gajenog sirka (Sorghum bicolor) sa simptomima truleži prizemnog dela stabla. Iz biljnog tkiva izolovane su monosporne kulture, čija je patogenost potvrđena pojavom simptoma na veštački inokulisanim biljkama sirka, a na osnovu morfoloških makroskopskih i mikroskopskih osobina identifikovan je Fusarium graminearum. Molekularna identifikacija obavljena je primenom lančane reakcije polimeraze (PCR, Polymerase Chain Reaction) uz korišćenje prajmera ef1/ef2 i amplifikaciju kodirajućeg proteinskog gena TEF 1-alfa. Sekvenca TEF gena odabranog izolata 535-10 (JF747146) je pokazala 98% do 99% nukleotidne identičnosti sa sekvencama 63 izolata Gibberella zeae deponovanih u NCBI bazi podataka. Amplifikacijom barkoding dela genoma F. graminearum izolata iz sirka dat je doprinos bržoj i preciznijoj identifikaciji i karakterizaciji vrsta roda Fusarium u Srbiji.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Molecular identification of Fusarium graminearum, sorghum pathogen in Serbia
T1  - Molekularna identifikacija izolata Fusarium graminearum, patogena sirka u Srbiji
EP  - 352
IS  - 2
SP  - 347
VL  - 48
DO  - 10.5937/ratpov1102347R
ER  - 

@article{
author = "Ristić, Danijela and Vučurović, Ana and Stanković, Ivana and Nikolić, Dušan and Berenji, Janoš and Krstić, Branka and Bulajić, Aleksandra",
year = "2011",
abstract = "A total of 39 samples of sorghum (Sorghum bicolor) with symptoms of stem and root rot were collected and analyzed during 2009-2011 in Bački Petrovac and Čantavir, Serbia. Monosporic cultures were isolated from stem tissue, their pathogenicity was confirmed by the development of symptoms on artificially inoculated sorghum plants, and they were identified on the basis of macroscopic and microscopic morphological features as Fusarium graminearum. Molecular identification was performed utilizing polimerase chain reaction (PCR) with primer pair ef1/ ef2 and by amplification of protein coding TEF 1-alpha gen. Sequence of TEF gene from the selected isolate 535- 10 (JF747146) showed 98-99% nucleotide identity with sequences of 63 Gibberella zeae isolates deposited in NCBI GenBank. Amplification of the barcoding region of F. graminearum genome of sorghum isolate, contributes to the fast and accurate identification and characterization of Fusarium species in Serbia., U periodu 2009-2011. na lokalitetima u Bačkom Petrovcu i Čantaviru prikupljeno je i analizirano 39 uzoraka biljaka gajenog sirka (Sorghum bicolor) sa simptomima truleži prizemnog dela stabla. Iz biljnog tkiva izolovane su monosporne kulture, čija je patogenost potvrđena pojavom simptoma na veštački inokulisanim biljkama sirka, a na osnovu morfoloških makroskopskih i mikroskopskih osobina identifikovan je Fusarium graminearum. Molekularna identifikacija obavljena je primenom lančane reakcije polimeraze (PCR, Polymerase Chain Reaction) uz korišćenje prajmera ef1/ef2 i amplifikaciju kodirajućeg proteinskog gena TEF 1-alfa. Sekvenca TEF gena odabranog izolata 535-10 (JF747146) je pokazala 98% do 99% nukleotidne identičnosti sa sekvencama 63 izolata Gibberella zeae deponovanih u NCBI bazi podataka. Amplifikacijom barkoding dela genoma F. graminearum izolata iz sirka dat je doprinos bržoj i preciznijoj identifikaciji i karakterizaciji vrsta roda Fusarium u Srbiji.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Molecular identification of Fusarium graminearum, sorghum pathogen in Serbia, Molekularna identifikacija izolata Fusarium graminearum, patogena sirka u Srbiji",
pages = "352-347",
number = "2",
volume = "48",
doi = "10.5937/ratpov1102347R"
}

Ristić, D., Vučurović, A., Stanković, I., Nikolić, D., Berenji, J., Krstić, B.,& Bulajić, A.. (2011). Molecular identification of Fusarium graminearum, sorghum pathogen in Serbia. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 48(2), 347-352.
https://doi.org/10.5937/ratpov1102347R

Ristić D, Vučurović A, Stanković I, Nikolić D, Berenji J, Krstić B, Bulajić A. Molecular identification of Fusarium graminearum, sorghum pathogen in Serbia. in Ratarstvo i povrtarstvo. 2011;48(2):347-352.
doi:10.5937/ratpov1102347R .

Ristić, Danijela, Vučurović, Ana, Stanković, Ivana, Nikolić, Dušan, Berenji, Janoš, Krstić, Branka, Bulajić, Aleksandra, "Molecular identification of Fusarium graminearum, sorghum pathogen in Serbia" in Ratarstvo i povrtarstvo, 48, no. 2 (2011):347-352,
https://doi.org/10.5937/ratpov1102347R . .

TY  - JOUR
AU  - Vučurović, Ana
AU  - Bulajić, Aleksandra
AU  - Stanković, Ivana
AU  - Ristić, Danijela
AU  - Berenji, Janoš
AU  - Krstić, Branka
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2539
AB  - Cucumber mosaic virus (CMV) is considered one of the most economically important plant viruses and has a worldwide distribution and a very wide host range including plants from family Cucurbitaceae. In Serbia, on cucurbits CMV was detected in single and mixed infections with Zucchini yellow mosaic virus (ZYMV) and Watermelon mosaic virus (WMV). Viruses, including CMV, are constantly present in cucurbit crops, but their frequency changes by year and locality. Surveys and sample collections were conducted in cucurbit crops in the period from 2008 to 2009 at 15 localities in Vojvodina province, and sample testing was carried out using the DAS-ELISA method and commercially available antisera for six economically most important cucurbit viruses. In 2008, a total of 51 samples were collected from 13 cucurbit crops of oilseed pumpkin Olinka variety, squash, and bottle gourd and CMV was detected in a total of 55% of tested samples with symptoms of viral infection. The most common infectious type was mixed infection with ZYMV and WMV (35.3%), and then mixed infection with ZYMV (17.7%) and WMV (2%). A total of 599 symptomatic samples of oilseed pumpkin Olinka variety, zucchini squash varieties Beogradska and Tosca, squash, and winter squash were collected in 15 cucurbits crops in 2009. CMV was present in 4.4% of total collected samples, in single infections in 1.3%, and in mixed with WMV or ZYMV in 1.3%, and 1.8%. Five CMV isolates were obtained by mechanical inoculations of N. glutinosa and one of them was selected for further biological characterization. Test plants which were described to be hosts of CMV expressed symptoms characteristic for those caused by CMV after inoculations by isolate 115-08. CMV specific primers Au1u/Au2d were used to amplify an 850 bp fragment using RT-PCR method. Amplified fragment encodes the entire viral coat protein (CP) gene and partial 5' and 3' UTRs of two selected CMV isolates. Amplified fragments were sequenced and deposited in the NCBI, where they were assigned accession numbers, HM065510 (115-08) and HM065509 (151-08). The sequences of CMV isolates from Serbia shared the highest nucleotide and amino acid identity with isolates from subgroup IA, from 99.5 to 97.4% and 99.1 to 97.4%, and the lowest identities were with the subgroup II isolates from 66.9 to 64, 5%, from 75.8 to 74.1%.
AB  - Virus mozaika krastavca (Cucumber mosaic virus, CMV) je veoma široko rasprostranjen i jedan je od ekonomski najznačajnijih virusa za poljoprivrednu proizvodnju, sa veoma širokim krugom domaćina u koji spada i veliki broj gajenih biljaka, među kojima i predstavnici familije Cucurbitaceae. Epidemiologija ovog virusa je veoma složena, zbog čega je i njegova kontrola otežana. Na tikvama u Srbiji CMV je detektovan u pojedinačnim i mešanim infekcijama sa virusom žutog mozaika cukinija (Zucchini yellow mosaic virus, ZYMV) i virusom mozaika lubenice (Watermelon mosaic virus, WMV). Virusi, pa tako i CMV, su stalno prisutni u usevima tikava, ali se njihova učestalost menja po godinama i lokalitetima. Pregledi terena i sakupljanje uzoraka tikava obavljeni su u periodu 2008-2009. godine na 15 lokaliteta gajenja tikava u Vojvodini, a testiranje sakupljenih uzoraka izvršeno je primenom DAS-ELISA metode i komercijalno dostupnih antiseruma za šest ekonomski najznačajnih virusa tikava u svetu. Tokom 2008. godine u 13 useva gajenih tikava sakupljen je ukupno 51 uzorak uljane tikve sorte Olinka, bundeve i vrga, a CMV je detektovan u ukupno 55% testiranih uzoraka sa simptomima virusnih zaraza. CMV je najčešće bio detektovan u mešanoj infekciji sa ZYMV i WMV (35,3%), a zatim u mešanoj infekciji sa ZYMV (17,7%) i sa WMV (2%). Ukupno 599 simptomatičnih uzoraka uljane tikve sorte Olinka, tikvica sorti Beogradska i Toska, bundeve i muskatne tikve sakupljeno je u 15 useva tokom 2009. godine. U sakupljenim uzorcima CMV je bio prisutan u ukupno 4,4% testiranih uzoraka. U pojedinačnim infekcijama u 1,3%, a u mešanim sa WMV ili ZYMV u 1,3%, odnosno 1,8% testiranih uzoraka. Mehaničkim inokulacijama N. glutinosa dobijeno je pet izolata CMV od kojih je jedan izabran za dalju biološku karakterizaciju. Odabrani izolat (115-08) izazvao je na test biljkama koje su opisane kao domaćini CMV pojavu karakterističnih simptoma. Specifičnim prajmerima CMV Au1u/Au2d uz upotrebu RT-PCR metode umnožen je deo genoma od oko 850 bp koji kodira ceo protein omotača virusa i delove 5' i 3' neprepisujućih regiona dva odabrana izolata CMV. Amplifikovani fragmenti su sekvencionirani i prijavljeni u NCBI, gde su im dodeljeni pristupni brojevi, HM065510 (115-08) i HM065509 (151-08). Sekvence CMV izolata iz Srbije dele najveću nukleotidnu i aminokiselinsku sličnost sa izolatima iz podgrupe IA, od 99,5 do 97,4%, odnosno 99,1 do 97,4%, a najmanju sa izolatima II podgrupe od 66,9 do 64,5%, odnosno od 75,8 do 74,1%.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Characterization of cucumber mosaic virus originating from cucurbits in Serbia
T1  - Karakterizacija virusa mozaika krastavca poreklom iz tikava u Srbiji
EP  - 336
IS  - 4
SP  - 325
VL  - 26
DO  - 10.2298/PIF1104325V
ER  - 

@article{
author = "Vučurović, Ana and Bulajić, Aleksandra and Stanković, Ivana and Ristić, Danijela and Berenji, Janoš and Krstić, Branka",
year = "2011",
abstract = "Cucumber mosaic virus (CMV) is considered one of the most economically important plant viruses and has a worldwide distribution and a very wide host range including plants from family Cucurbitaceae. In Serbia, on cucurbits CMV was detected in single and mixed infections with Zucchini yellow mosaic virus (ZYMV) and Watermelon mosaic virus (WMV). Viruses, including CMV, are constantly present in cucurbit crops, but their frequency changes by year and locality. Surveys and sample collections were conducted in cucurbit crops in the period from 2008 to 2009 at 15 localities in Vojvodina province, and sample testing was carried out using the DAS-ELISA method and commercially available antisera for six economically most important cucurbit viruses. In 2008, a total of 51 samples were collected from 13 cucurbit crops of oilseed pumpkin Olinka variety, squash, and bottle gourd and CMV was detected in a total of 55% of tested samples with symptoms of viral infection. The most common infectious type was mixed infection with ZYMV and WMV (35.3%), and then mixed infection with ZYMV (17.7%) and WMV (2%). A total of 599 symptomatic samples of oilseed pumpkin Olinka variety, zucchini squash varieties Beogradska and Tosca, squash, and winter squash were collected in 15 cucurbits crops in 2009. CMV was present in 4.4% of total collected samples, in single infections in 1.3%, and in mixed with WMV or ZYMV in 1.3%, and 1.8%. Five CMV isolates were obtained by mechanical inoculations of N. glutinosa and one of them was selected for further biological characterization. Test plants which were described to be hosts of CMV expressed symptoms characteristic for those caused by CMV after inoculations by isolate 115-08. CMV specific primers Au1u/Au2d were used to amplify an 850 bp fragment using RT-PCR method. Amplified fragment encodes the entire viral coat protein (CP) gene and partial 5' and 3' UTRs of two selected CMV isolates. Amplified fragments were sequenced and deposited in the NCBI, where they were assigned accession numbers, HM065510 (115-08) and HM065509 (151-08). The sequences of CMV isolates from Serbia shared the highest nucleotide and amino acid identity with isolates from subgroup IA, from 99.5 to 97.4% and 99.1 to 97.4%, and the lowest identities were with the subgroup II isolates from 66.9 to 64, 5%, from 75.8 to 74.1%., Virus mozaika krastavca (Cucumber mosaic virus, CMV) je veoma široko rasprostranjen i jedan je od ekonomski najznačajnijih virusa za poljoprivrednu proizvodnju, sa veoma širokim krugom domaćina u koji spada i veliki broj gajenih biljaka, među kojima i predstavnici familije Cucurbitaceae. Epidemiologija ovog virusa je veoma složena, zbog čega je i njegova kontrola otežana. Na tikvama u Srbiji CMV je detektovan u pojedinačnim i mešanim infekcijama sa virusom žutog mozaika cukinija (Zucchini yellow mosaic virus, ZYMV) i virusom mozaika lubenice (Watermelon mosaic virus, WMV). Virusi, pa tako i CMV, su stalno prisutni u usevima tikava, ali se njihova učestalost menja po godinama i lokalitetima. Pregledi terena i sakupljanje uzoraka tikava obavljeni su u periodu 2008-2009. godine na 15 lokaliteta gajenja tikava u Vojvodini, a testiranje sakupljenih uzoraka izvršeno je primenom DAS-ELISA metode i komercijalno dostupnih antiseruma za šest ekonomski najznačajnih virusa tikava u svetu. Tokom 2008. godine u 13 useva gajenih tikava sakupljen je ukupno 51 uzorak uljane tikve sorte Olinka, bundeve i vrga, a CMV je detektovan u ukupno 55% testiranih uzoraka sa simptomima virusnih zaraza. CMV je najčešće bio detektovan u mešanoj infekciji sa ZYMV i WMV (35,3%), a zatim u mešanoj infekciji sa ZYMV (17,7%) i sa WMV (2%). Ukupno 599 simptomatičnih uzoraka uljane tikve sorte Olinka, tikvica sorti Beogradska i Toska, bundeve i muskatne tikve sakupljeno je u 15 useva tokom 2009. godine. U sakupljenim uzorcima CMV je bio prisutan u ukupno 4,4% testiranih uzoraka. U pojedinačnim infekcijama u 1,3%, a u mešanim sa WMV ili ZYMV u 1,3%, odnosno 1,8% testiranih uzoraka. Mehaničkim inokulacijama N. glutinosa dobijeno je pet izolata CMV od kojih je jedan izabran za dalju biološku karakterizaciju. Odabrani izolat (115-08) izazvao je na test biljkama koje su opisane kao domaćini CMV pojavu karakterističnih simptoma. Specifičnim prajmerima CMV Au1u/Au2d uz upotrebu RT-PCR metode umnožen je deo genoma od oko 850 bp koji kodira ceo protein omotača virusa i delove 5' i 3' neprepisujućih regiona dva odabrana izolata CMV. Amplifikovani fragmenti su sekvencionirani i prijavljeni u NCBI, gde su im dodeljeni pristupni brojevi, HM065510 (115-08) i HM065509 (151-08). Sekvence CMV izolata iz Srbije dele najveću nukleotidnu i aminokiselinsku sličnost sa izolatima iz podgrupe IA, od 99,5 do 97,4%, odnosno 99,1 do 97,4%, a najmanju sa izolatima II podgrupe od 66,9 do 64,5%, odnosno od 75,8 do 74,1%.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Characterization of cucumber mosaic virus originating from cucurbits in Serbia, Karakterizacija virusa mozaika krastavca poreklom iz tikava u Srbiji",
pages = "336-325",
number = "4",
volume = "26",
doi = "10.2298/PIF1104325V"
}

Vučurović, A., Bulajić, A., Stanković, I., Ristić, D., Berenji, J.,& Krstić, B.. (2011). Characterization of cucumber mosaic virus originating from cucurbits in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 26(4), 325-336.
https://doi.org/10.2298/PIF1104325V

Vučurović A, Bulajić A, Stanković I, Ristić D, Berenji J, Krstić B. Characterization of cucumber mosaic virus originating from cucurbits in Serbia. in Pesticidi i fitomedicina. 2011;26(4):325-336.
doi:10.2298/PIF1104325V .

Vučurović, Ana, Bulajić, Aleksandra, Stanković, Ivana, Ristić, Danijela, Berenji, Janoš, Krstić, Branka, "Characterization of cucumber mosaic virus originating from cucurbits in Serbia" in Pesticidi i fitomedicina, 26, no. 4 (2011):325-336,
https://doi.org/10.2298/PIF1104325V . .

TY  - JOUR
AU  - Stanković, Ivana
AU  - Vučurović, Ana
AU  - Bulajić, Aleksandra
AU  - Ristić, Danijela
AU  - Berenji, Janoš
AU  - Krstić, Branka
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2532
AB  - Three-year investigation of the presence and distribution of tobacco viruses in Serbia revealed that Alfalfa mosaic virus (AMV) appeared every year with different frequency in tobacco crops. During 2008, the presence of AMV was detected in most of the tested samples (58.82%) and it was the second most common compared to all other viruses which presence was confirmed in Serbia. In 2006 and 2007, AMV was detected in a significantly lower percentage (2.80% and 13.64%, respectively). This study showed that Alfalfa mosaic virus was more commonly found in multiple infections with two, three or even four detected viruses. Single infections were detected only in 2006, in one tobacco field in the locality of Futog. During this investigation, a rapid and simple protocol was optimized and developed for molecular detection of AMV in tobacco leaves, using primers CPAMV1/CPAMV2 and commercially available kits for total RNA extraction as well as for RT-PCR (reverse transcription - polymerase chain reaction). Using RT-PCR and these primers that flank the AMV coat protein gene, a DNA fragment of 751 bp was amplified, sequenced, and compared with the sequences available in GenBank database. The sequence of isolate 196-08 (GenBank Acc. No. FJ527749) proved to be identical at the nucleotide level of 99 to 93% with those from other parts of the world. Phylogenetic analysis of 27 isolates based on 528 bp sequences of the coat protein gene did not show correlation of the isolates with their geographic origin or plant host and showed that these isolates fall into four molecular groups of strains. Serbian AMV isolate from tobacco belongs to group IV, the group that includes most of the isolates selected for phylogenetic analysis.
AB  - Trogodišnjim proučavanjima pojave i rasprostranjenosti virusa u usevu duvana u Srbiji utvrđeno je da se virus mozaika lucerke (Alfalfa mosaic virus, AMV) javlja svake godine sa različitom učestalošću. Prisustvo AMV ustanovljeno je u najvećem broju testiranih uzoraka (58,82%) tokom 2008. godine kada je bio drugi po zastupljenosti u odnosu na sve ostale viruse čije je prisustvo utvrđeno u Srbiji. AMV je detektovan u znatno nižem procentu 2006. (2,80%) i 2007. godine (13,64%). Tokom ovih istraživanja virus mozaika lucerke češće je bio prisutan u mešanim infekcijama sa dva, tri ili čak četiri detektovana virusa. Prisustvo pojedinačne zaraze utvrđeno je samo 2006. godine u jednom usevu duvana na lokalitetu Futog. U toku ovih istraživanja razvijen je i optimiziran brz i precizan protokol za molekularnu detekciju AMV u lišću duvana primenom specifičnih prajmera CPAMV1/CPAMV2 i komercijalnih kitova za ekstrakciju ukupnih RNK i RT-PCR (reverzna transkripcija praćena lančanom reakcijom polimeraze). Korišćenjem ovih prajmera, koji omogućavaju umnožavanje gena za protein omotača, umnožen je fragment dužine 751 bp, sekvencioniran i upoređen sa sekvencama dostupnim u GenBank bazi podataka. Dobijena sekvenca izolata 196-08 (GenBank Acc. No. FJ527749) pokazala je 99-93% nukleotidne identičnosti sa izolatima AMV iz drugih delova sveta. Filogenetske analize 27 izolata, na osnovu 528 bp sekvence gena za protein omotača, nisu ukazale na korelaciju sa geografskim poreklom izolata ili biljkom domaćinom, a pokazale su grupisanje izolata u četiri molekularne grupe sojeva. Izolat AMV iz duvana iz Srbije pripada grupi IV, koja obuhvata većinu izolata odabranih za filogenetsku analizu.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Presence and molecular characterization of alfalfa mosaic virus on tobacco in Serbia
T1  - Prisustvo i molekularna karakterizacija virusa mozaika lucerke u usevu duvana u Srbiji
EP  - 243
IS  - 3
SP  - 229
VL  - 26
DO  - 10.2298/PIF1103229S
ER  - 

@article{
author = "Stanković, Ivana and Vučurović, Ana and Bulajić, Aleksandra and Ristić, Danijela and Berenji, Janoš and Krstić, Branka",
year = "2011",
abstract = "Three-year investigation of the presence and distribution of tobacco viruses in Serbia revealed that Alfalfa mosaic virus (AMV) appeared every year with different frequency in tobacco crops. During 2008, the presence of AMV was detected in most of the tested samples (58.82%) and it was the second most common compared to all other viruses which presence was confirmed in Serbia. In 2006 and 2007, AMV was detected in a significantly lower percentage (2.80% and 13.64%, respectively). This study showed that Alfalfa mosaic virus was more commonly found in multiple infections with two, three or even four detected viruses. Single infections were detected only in 2006, in one tobacco field in the locality of Futog. During this investigation, a rapid and simple protocol was optimized and developed for molecular detection of AMV in tobacco leaves, using primers CPAMV1/CPAMV2 and commercially available kits for total RNA extraction as well as for RT-PCR (reverse transcription - polymerase chain reaction). Using RT-PCR and these primers that flank the AMV coat protein gene, a DNA fragment of 751 bp was amplified, sequenced, and compared with the sequences available in GenBank database. The sequence of isolate 196-08 (GenBank Acc. No. FJ527749) proved to be identical at the nucleotide level of 99 to 93% with those from other parts of the world. Phylogenetic analysis of 27 isolates based on 528 bp sequences of the coat protein gene did not show correlation of the isolates with their geographic origin or plant host and showed that these isolates fall into four molecular groups of strains. Serbian AMV isolate from tobacco belongs to group IV, the group that includes most of the isolates selected for phylogenetic analysis., Trogodišnjim proučavanjima pojave i rasprostranjenosti virusa u usevu duvana u Srbiji utvrđeno je da se virus mozaika lucerke (Alfalfa mosaic virus, AMV) javlja svake godine sa različitom učestalošću. Prisustvo AMV ustanovljeno je u najvećem broju testiranih uzoraka (58,82%) tokom 2008. godine kada je bio drugi po zastupljenosti u odnosu na sve ostale viruse čije je prisustvo utvrđeno u Srbiji. AMV je detektovan u znatno nižem procentu 2006. (2,80%) i 2007. godine (13,64%). Tokom ovih istraživanja virus mozaika lucerke češće je bio prisutan u mešanim infekcijama sa dva, tri ili čak četiri detektovana virusa. Prisustvo pojedinačne zaraze utvrđeno je samo 2006. godine u jednom usevu duvana na lokalitetu Futog. U toku ovih istraživanja razvijen je i optimiziran brz i precizan protokol za molekularnu detekciju AMV u lišću duvana primenom specifičnih prajmera CPAMV1/CPAMV2 i komercijalnih kitova za ekstrakciju ukupnih RNK i RT-PCR (reverzna transkripcija praćena lančanom reakcijom polimeraze). Korišćenjem ovih prajmera, koji omogućavaju umnožavanje gena za protein omotača, umnožen je fragment dužine 751 bp, sekvencioniran i upoređen sa sekvencama dostupnim u GenBank bazi podataka. Dobijena sekvenca izolata 196-08 (GenBank Acc. No. FJ527749) pokazala je 99-93% nukleotidne identičnosti sa izolatima AMV iz drugih delova sveta. Filogenetske analize 27 izolata, na osnovu 528 bp sekvence gena za protein omotača, nisu ukazale na korelaciju sa geografskim poreklom izolata ili biljkom domaćinom, a pokazale su grupisanje izolata u četiri molekularne grupe sojeva. Izolat AMV iz duvana iz Srbije pripada grupi IV, koja obuhvata većinu izolata odabranih za filogenetsku analizu.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Presence and molecular characterization of alfalfa mosaic virus on tobacco in Serbia, Prisustvo i molekularna karakterizacija virusa mozaika lucerke u usevu duvana u Srbiji",
pages = "243-229",
number = "3",
volume = "26",
doi = "10.2298/PIF1103229S"
}

Stanković, I., Vučurović, A., Bulajić, A., Ristić, D., Berenji, J.,& Krstić, B.. (2011). Presence and molecular characterization of alfalfa mosaic virus on tobacco in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 26(3), 229-243.
https://doi.org/10.2298/PIF1103229S

Stanković I, Vučurović A, Bulajić A, Ristić D, Berenji J, Krstić B. Presence and molecular characterization of alfalfa mosaic virus on tobacco in Serbia. in Pesticidi i fitomedicina. 2011;26(3):229-243.
doi:10.2298/PIF1103229S .

Stanković, Ivana, Vučurović, Ana, Bulajić, Aleksandra, Ristić, Danijela, Berenji, Janoš, Krstić, Branka, "Presence and molecular characterization of alfalfa mosaic virus on tobacco in Serbia" in Pesticidi i fitomedicina, 26, no. 3 (2011):229-243,
https://doi.org/10.2298/PIF1103229S . .

TY  - JOUR
AU  - Bulajić, Aleksandra
AU  - Vučurović, Ana
AU  - Stanković, Ivana
AU  - Ristić, Danijela
AU  - Berenji, Janoš
AU  - Krstić, Branka
PY  - 2010
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2252
AB  - As there is a growing frequency of viral plant diseases in epidemic proportions, the possibilities for successful control are constantly being explored. Despite the fact that integral and simultaneous employment of numerous control measures may contribute to the decreasing amount of yield losses, especially concerning non-persistently aphid-transmitted viruses, these measures are often not efficient enough. Research into the basis of resistance to viral infection and principles of its inheritance, introduction of sources of resistance in susceptible genotypes, by conventional or genetic manipulations, are very intensive for cucurbit crops, especially pumpkins. Pumpkin crops are being endangered by a great number of different viruses, among which the Zucchini yellow mosaic virus, (ZYMV), Watermelon mosaic virus (WMV) and Cucumber mosaic virus (CMV) are present every year in Serbia, frequently causing epidemics. The majority of pumpkin cultivars are not resistant or tolerant to viral infections, but sources of resistance have been identified in various related species. So far, the identified sources of resistance to the ZYMV are found in Cucurbita moschata and Citrullus lanatus var. lanatus genotypes and consist of one or several major dominant genes of resistance. It is a similar case with WMV, although the sources of dominant major genes are identified in C. lanatus and C. colocynthis. The sources of resistance to CMV in the form of one dominant gene have been identified in the genotype C. moschata, although the introduction of this gene by conventional means proved to be very difficult. Besides the aforementioned, substantial efforts are being made in developing genotypes with multiple resistance against several viruses and even other pathogens, as well as genotypes with resistance to the most significant plant aphid species, through mechanisms of antixenosis or antibiosis. The other way of obtaining resistant genotypes includes genetic manipulation. Genetically modified resistant pumpkins have been among the first successfully developed crops. Genotypes with pathogen derived resistance can already be found in commercially grown pumpkins in some parts of the world, and they have been developed by introducing the coat protein gene of one, two or all three viruses which are the most frequent, ZYMV, WMV and CMV. Yet, this approach to the control of pumpkin viral diseases is related to possible negative consequences, mostly through the already detected gene transfer to wild plants and development of resistant transgenic weeds of unpredictable impact on the environment. Improved host plant genetic resistance to viral infections or biological vectors, developed by conventional or genetic engineering methods, represents the most dynamic and prominent field of research. It is economically and ecologically the most justified approach to the control of pumpkin and other plant diseases caused by viruses non-persistently transmitted by aphids.
AB  - Kako je pojava brojnih viroza gajenih biljaka u epidemijskim razmerama sve češća, mogućnosti za uspešnu kontrolu stalno se ispituju. Integralna i istovremena primena brojnih pojedinačnih mera kontrole, naročito u slučaju virusa koji se prenose biljnim vašima na neperzistentan način, mogu da doprinesu smanjenju šteta, ali često nisu dovoljno efikasne. Proučavanja osnova otpornosti i načina nasleđivanja, unošenje izvora otpornosti u osetljive genotipove, konvencionalnim ili genetičkim manipulacijama, vrlo su intenzivna kod vrežastih kultura, posebno tikve. Tikve ugrožava veliki broj raznorodnih virusa od kojih se u Srbiji svake godine javljaju virus žutog mozaika cukinija (Zucchini yellow mosaic virus, ZYMV), virus mozaika lubenice (Watermelon mosaic virus, WMV) i virus mozaika krastavca (Cucumber mosaic virus, CMV), a pojedinih godina izazivaju epidemije. Većina sorti gajenih tikava ne poseduje otpornost ili tolerantnost na virusne zaraze, ali izvori otpornosti identifikovani su u različitim srodnim vrstama. Do sada identifikovani izvori otpornosti na ZYMV nalaze se u genotipovima Cucurbita moschata i Citrullus lanatus var. lanatus i obuhvataju jedan ili nekoliko major gena rezistentnosti koji se nasleđuju dominantno. Slična situacija je i sa WMV, mada su izvori dominantnih major gena identifikovani u C. lanatus i C. colocynthis. Izvor otpornosti na CMV u vidu jednog dominantnog gena identifikovan je u genotipu C. moschata, mada je unošenje ovog gena konvencionalnim putem bilo veoma teško. Pored toga, veliki napori ulažu se u dobijenje genotipova sa istovremenom otpornošću na više virusa čak i drugih patogena, kao i genotipova koji ispoljavaju otpornost na najznačajnije vrste biljnih vaši, kroz mehanizme antiksenoze ili antibioze. Drugi pravac dobijanja otpornih genotipova podrazumeva genetičke manipulacije. Genetski modifikovane otporne tikve su među prvima, od svih gajenih biljaka, uspešno razvijene. Genotipovi sa PD (pathogen derived) otpornošću već se nalaze u komercijalnoj proizvodnji tikava u nekim delovima sveta i dobijeni su unošenjem gena za CP (coat protein) jednog, dva ili sva tri u svetu najraširenija virusa, ZYMV, WMW i CMV. Ipak, ovaj prilaz kontroli virusnih oboljenja tikava vezan je za uočene moguće negativne posledice, pre svega kroz već registrovan transfer gena u biljke spontane flore i nastanak otpornih transgenih korova nepredvidljivog ponašanja i značaja u prirodi. Poboljšana genetička otpornost biljke domaćina bilo na infekciju virusima, bilo na biološke vektore, dobijena konvencionalnim ili metodama genetičkog inženjeringa predstavlja najdinamičnije i najperspektivnije polje istraživanja kao ekonomski i ekološki najopravdaniji pristup kontroli oboljenja tikava i drugih biljaka koje izazivaju virusi koji se neperzistetno prenose biljnim vašima.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Novel approaches to implementation of pumpkin resistance in control of viral diseases
T1  - Novi pravci primene otpornosti tikava u kontroli virusnih oboljenja
EP  - 211
IS  - 3
SP  - 201
VL  - 25
DO  - 10.2298/PIF1003201B
ER  - 

@article{
author = "Bulajić, Aleksandra and Vučurović, Ana and Stanković, Ivana and Ristić, Danijela and Berenji, Janoš and Krstić, Branka",
year = "2010",
abstract = "As there is a growing frequency of viral plant diseases in epidemic proportions, the possibilities for successful control are constantly being explored. Despite the fact that integral and simultaneous employment of numerous control measures may contribute to the decreasing amount of yield losses, especially concerning non-persistently aphid-transmitted viruses, these measures are often not efficient enough. Research into the basis of resistance to viral infection and principles of its inheritance, introduction of sources of resistance in susceptible genotypes, by conventional or genetic manipulations, are very intensive for cucurbit crops, especially pumpkins. Pumpkin crops are being endangered by a great number of different viruses, among which the Zucchini yellow mosaic virus, (ZYMV), Watermelon mosaic virus (WMV) and Cucumber mosaic virus (CMV) are present every year in Serbia, frequently causing epidemics. The majority of pumpkin cultivars are not resistant or tolerant to viral infections, but sources of resistance have been identified in various related species. So far, the identified sources of resistance to the ZYMV are found in Cucurbita moschata and Citrullus lanatus var. lanatus genotypes and consist of one or several major dominant genes of resistance. It is a similar case with WMV, although the sources of dominant major genes are identified in C. lanatus and C. colocynthis. The sources of resistance to CMV in the form of one dominant gene have been identified in the genotype C. moschata, although the introduction of this gene by conventional means proved to be very difficult. Besides the aforementioned, substantial efforts are being made in developing genotypes with multiple resistance against several viruses and even other pathogens, as well as genotypes with resistance to the most significant plant aphid species, through mechanisms of antixenosis or antibiosis. The other way of obtaining resistant genotypes includes genetic manipulation. Genetically modified resistant pumpkins have been among the first successfully developed crops. Genotypes with pathogen derived resistance can already be found in commercially grown pumpkins in some parts of the world, and they have been developed by introducing the coat protein gene of one, two or all three viruses which are the most frequent, ZYMV, WMV and CMV. Yet, this approach to the control of pumpkin viral diseases is related to possible negative consequences, mostly through the already detected gene transfer to wild plants and development of resistant transgenic weeds of unpredictable impact on the environment. Improved host plant genetic resistance to viral infections or biological vectors, developed by conventional or genetic engineering methods, represents the most dynamic and prominent field of research. It is economically and ecologically the most justified approach to the control of pumpkin and other plant diseases caused by viruses non-persistently transmitted by aphids., Kako je pojava brojnih viroza gajenih biljaka u epidemijskim razmerama sve češća, mogućnosti za uspešnu kontrolu stalno se ispituju. Integralna i istovremena primena brojnih pojedinačnih mera kontrole, naročito u slučaju virusa koji se prenose biljnim vašima na neperzistentan način, mogu da doprinesu smanjenju šteta, ali često nisu dovoljno efikasne. Proučavanja osnova otpornosti i načina nasleđivanja, unošenje izvora otpornosti u osetljive genotipove, konvencionalnim ili genetičkim manipulacijama, vrlo su intenzivna kod vrežastih kultura, posebno tikve. Tikve ugrožava veliki broj raznorodnih virusa od kojih se u Srbiji svake godine javljaju virus žutog mozaika cukinija (Zucchini yellow mosaic virus, ZYMV), virus mozaika lubenice (Watermelon mosaic virus, WMV) i virus mozaika krastavca (Cucumber mosaic virus, CMV), a pojedinih godina izazivaju epidemije. Većina sorti gajenih tikava ne poseduje otpornost ili tolerantnost na virusne zaraze, ali izvori otpornosti identifikovani su u različitim srodnim vrstama. Do sada identifikovani izvori otpornosti na ZYMV nalaze se u genotipovima Cucurbita moschata i Citrullus lanatus var. lanatus i obuhvataju jedan ili nekoliko major gena rezistentnosti koji se nasleđuju dominantno. Slična situacija je i sa WMV, mada su izvori dominantnih major gena identifikovani u C. lanatus i C. colocynthis. Izvor otpornosti na CMV u vidu jednog dominantnog gena identifikovan je u genotipu C. moschata, mada je unošenje ovog gena konvencionalnim putem bilo veoma teško. Pored toga, veliki napori ulažu se u dobijenje genotipova sa istovremenom otpornošću na više virusa čak i drugih patogena, kao i genotipova koji ispoljavaju otpornost na najznačajnije vrste biljnih vaši, kroz mehanizme antiksenoze ili antibioze. Drugi pravac dobijanja otpornih genotipova podrazumeva genetičke manipulacije. Genetski modifikovane otporne tikve su među prvima, od svih gajenih biljaka, uspešno razvijene. Genotipovi sa PD (pathogen derived) otpornošću već se nalaze u komercijalnoj proizvodnji tikava u nekim delovima sveta i dobijeni su unošenjem gena za CP (coat protein) jednog, dva ili sva tri u svetu najraširenija virusa, ZYMV, WMW i CMV. Ipak, ovaj prilaz kontroli virusnih oboljenja tikava vezan je za uočene moguće negativne posledice, pre svega kroz već registrovan transfer gena u biljke spontane flore i nastanak otpornih transgenih korova nepredvidljivog ponašanja i značaja u prirodi. Poboljšana genetička otpornost biljke domaćina bilo na infekciju virusima, bilo na biološke vektore, dobijena konvencionalnim ili metodama genetičkog inženjeringa predstavlja najdinamičnije i najperspektivnije polje istraživanja kao ekonomski i ekološki najopravdaniji pristup kontroli oboljenja tikava i drugih biljaka koje izazivaju virusi koji se neperzistetno prenose biljnim vašima.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Novel approaches to implementation of pumpkin resistance in control of viral diseases, Novi pravci primene otpornosti tikava u kontroli virusnih oboljenja",
pages = "211-201",
number = "3",
volume = "25",
doi = "10.2298/PIF1003201B"
}

Bulajić, A., Vučurović, A., Stanković, I., Ristić, D., Berenji, J.,& Krstić, B.. (2010). Novel approaches to implementation of pumpkin resistance in control of viral diseases. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 25(3), 201-211.
https://doi.org/10.2298/PIF1003201B

Bulajić A, Vučurović A, Stanković I, Ristić D, Berenji J, Krstić B. Novel approaches to implementation of pumpkin resistance in control of viral diseases. in Pesticidi i fitomedicina. 2010;25(3):201-211.
doi:10.2298/PIF1003201B .

Bulajić, Aleksandra, Vučurović, Ana, Stanković, Ivana, Ristić, Danijela, Berenji, Janoš, Krstić, Branka, "Novel approaches to implementation of pumpkin resistance in control of viral diseases" in Pesticidi i fitomedicina, 25, no. 3 (2010):201-211,
https://doi.org/10.2298/PIF1003201B . .

TY  - JOUR
AU  - Vučurović, Ana
AU  - Bulajić, Aleksandra
AU  - Stanković, Ivana
AU  - Ristić, Danijela
AU  - Berenji, Janoš
AU  - Krstić, Branka
PY  - 2010
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2251
AB  - Watermelon mosaic virus (WMV) is widespread in cucurbit crops, most commonly occuring in temperate and Mediterranean regions. In Serbia WMV has been detected in single and mixed infections with Zucchini yellow mosaic virus and Cucumber mosaic virus in field-grown pumpkin and squash crops. Among pumpkin-affecting viruses WMV is the most frequent one, both by the number of localities and its incidence at each location. During the growing season of 2009, samples from 583 plants of Cucurbita pepo cvs. Olinka, Belgrade zucchini and Tosca (Zucchini group), as well as from C. maxima and C. moschata showing symptoms of virus infection were collected from 12 commercial fields at eight localities and analyzed by DAS-ELISA using polyclonal antisera specific to six most important cucurbit viruses. Interestingly, WMV was detected at fewer sites and had lower incidence rate than in two previous years. In single infections, WMV was found in 11% of tested plants in three fields; in mixed infections with ZYMV, it was recorded in 9.9% of plants in five fields and with CMV in only 0.2% in one field. The partial coat protein gene and 3' non-translated region from two representative isolates of WMV originating from different localities and host plant species were amplified by RT-PCR, sequenced, and compared with the sequences available in GenBank database. The PCR-amplified fragment of predicted size of approximately 1017 bp was obtained. The sequences of isolates 137-08 (Acc. No. GQ259958) and 159-08 (GU144020) proved to be 94-99% identical at the nucleotide level with those from other parts of the world. The sequences of these two isolates differed from each other only at two nucleotide positions, without any amino acid substitution. Phylogenetic analysis of 57 isolates based on 750 bp sequences of the coat protein gene showed no correlation between isolates and their geographic origin, and it also indicated that these isolates fell into three molecular groups of strains. Serbian WMV isolates belong to group I, the most widespread group of isolates of this virus in the world.
AB  - Virus mozaika lubenice (Watermelon mosaic virus, WMV) široko je rasprostranjen u usevima vrežastih kultura, mada se najčešće javlja na Mediteranu i regionima sa umerenom klimom. U Srbiji, WMV je detektovan u pojedinačnim i mešanim infekcijama sa virusom žutog mozaika cukinija (Zucchini yellow mosaic virus, ZYMV) i virusom mozaika krastavca (Cucumber mosaic virus, CMV) u usevima tikava i bundeva gajenim na otvorenom polju. Od virusa tikava, WMV je često prevalentan virus, kako po broju lokaliteta u kojima je dokazan, tako i po učestalosti u samom usevu. Iz ukupno 12 komercijalnih useva na osam lokaliteta, 2009. godine sakupljeno je 583 biljaka Cucurbita pepo cvs. Olinka, Beogradska tikvica i Tosca (cukini forma), kao i C. maxima i C. moschata, sa simptomima virusnih zaraza, koje su analizirane na prisustvo šest najznačajnijih virusa tikava primenom DAS-ELISA metode. WMV je detektovan na manjem broju lokaliteta i u manjoj učestalosti u poređenju sa prethodne dve vegetacije. U pojedinačnim infekcijama, WMV je detektovan u 11% testiranih biljaka u tri useva, a u mešanim sa ZYMV u 9,9% testiranih biljaka u pet useva i sa CMV u 0,2% testiranih biljaka u jednom usevu. Deo gena za protein omotača virusa i deo 3' neprepisujućeg regiona dva odabrana izolata WMV poreklom iz različitih lokaliteta i biljaka domaćina umnožen je primenom RT-PCR metode, sekvencioniran i upoređen sa sekvencama dostupnim u GenBank bazi podataka. Dobijen je PCR produkt očekivane veličine od 1017 bp. Sekvence izolata 137-08 (Acc. No. GQ259958) i 159-08 (GU144020) pokazale su 94-99% nukleotidne identičnosti sa izolatima WMV iz drugih delova sveta. Sekvence ova dva izolata razlikovala su se jedna od druge samo u dva nukleotidna mesta što nije imalo za posledicu aminokiselinske supstitucije. Filogenetske analize 57 izolata, na osnovu 750 bp sekvence gena za protein omotača, nisu ukazale na korelaciju sa geografskim poreklom izolata, a pokazala su grupisanje izolata u tri molekularne grupe sojeva. Izolati WMV iz Srbije pripadaju grupi I, grupi koja obuhvata najrasprostranjenije izolate ovog virusa u svetu.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Frequency and molecular characterization of watermelon mosaic virus from Serbia
T1  - Učestalost pojave i molekularna karakterizacija virusa mozaika lubenice poreklom iz Srbije
EP  - 230
IS  - 3
SP  - 213
VL  - 25
DO  - 10.2298/PIF1003213V
ER  - 

@article{
author = "Vučurović, Ana and Bulajić, Aleksandra and Stanković, Ivana and Ristić, Danijela and Berenji, Janoš and Krstić, Branka",
year = "2010",
abstract = "Watermelon mosaic virus (WMV) is widespread in cucurbit crops, most commonly occuring in temperate and Mediterranean regions. In Serbia WMV has been detected in single and mixed infections with Zucchini yellow mosaic virus and Cucumber mosaic virus in field-grown pumpkin and squash crops. Among pumpkin-affecting viruses WMV is the most frequent one, both by the number of localities and its incidence at each location. During the growing season of 2009, samples from 583 plants of Cucurbita pepo cvs. Olinka, Belgrade zucchini and Tosca (Zucchini group), as well as from C. maxima and C. moschata showing symptoms of virus infection were collected from 12 commercial fields at eight localities and analyzed by DAS-ELISA using polyclonal antisera specific to six most important cucurbit viruses. Interestingly, WMV was detected at fewer sites and had lower incidence rate than in two previous years. In single infections, WMV was found in 11% of tested plants in three fields; in mixed infections with ZYMV, it was recorded in 9.9% of plants in five fields and with CMV in only 0.2% in one field. The partial coat protein gene and 3' non-translated region from two representative isolates of WMV originating from different localities and host plant species were amplified by RT-PCR, sequenced, and compared with the sequences available in GenBank database. The PCR-amplified fragment of predicted size of approximately 1017 bp was obtained. The sequences of isolates 137-08 (Acc. No. GQ259958) and 159-08 (GU144020) proved to be 94-99% identical at the nucleotide level with those from other parts of the world. The sequences of these two isolates differed from each other only at two nucleotide positions, without any amino acid substitution. Phylogenetic analysis of 57 isolates based on 750 bp sequences of the coat protein gene showed no correlation between isolates and their geographic origin, and it also indicated that these isolates fell into three molecular groups of strains. Serbian WMV isolates belong to group I, the most widespread group of isolates of this virus in the world., Virus mozaika lubenice (Watermelon mosaic virus, WMV) široko je rasprostranjen u usevima vrežastih kultura, mada se najčešće javlja na Mediteranu i regionima sa umerenom klimom. U Srbiji, WMV je detektovan u pojedinačnim i mešanim infekcijama sa virusom žutog mozaika cukinija (Zucchini yellow mosaic virus, ZYMV) i virusom mozaika krastavca (Cucumber mosaic virus, CMV) u usevima tikava i bundeva gajenim na otvorenom polju. Od virusa tikava, WMV je često prevalentan virus, kako po broju lokaliteta u kojima je dokazan, tako i po učestalosti u samom usevu. Iz ukupno 12 komercijalnih useva na osam lokaliteta, 2009. godine sakupljeno je 583 biljaka Cucurbita pepo cvs. Olinka, Beogradska tikvica i Tosca (cukini forma), kao i C. maxima i C. moschata, sa simptomima virusnih zaraza, koje su analizirane na prisustvo šest najznačajnijih virusa tikava primenom DAS-ELISA metode. WMV je detektovan na manjem broju lokaliteta i u manjoj učestalosti u poređenju sa prethodne dve vegetacije. U pojedinačnim infekcijama, WMV je detektovan u 11% testiranih biljaka u tri useva, a u mešanim sa ZYMV u 9,9% testiranih biljaka u pet useva i sa CMV u 0,2% testiranih biljaka u jednom usevu. Deo gena za protein omotača virusa i deo 3' neprepisujućeg regiona dva odabrana izolata WMV poreklom iz različitih lokaliteta i biljaka domaćina umnožen je primenom RT-PCR metode, sekvencioniran i upoređen sa sekvencama dostupnim u GenBank bazi podataka. Dobijen je PCR produkt očekivane veličine od 1017 bp. Sekvence izolata 137-08 (Acc. No. GQ259958) i 159-08 (GU144020) pokazale su 94-99% nukleotidne identičnosti sa izolatima WMV iz drugih delova sveta. Sekvence ova dva izolata razlikovala su se jedna od druge samo u dva nukleotidna mesta što nije imalo za posledicu aminokiselinske supstitucije. Filogenetske analize 57 izolata, na osnovu 750 bp sekvence gena za protein omotača, nisu ukazale na korelaciju sa geografskim poreklom izolata, a pokazala su grupisanje izolata u tri molekularne grupe sojeva. Izolati WMV iz Srbije pripadaju grupi I, grupi koja obuhvata najrasprostranjenije izolate ovog virusa u svetu.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Frequency and molecular characterization of watermelon mosaic virus from Serbia, Učestalost pojave i molekularna karakterizacija virusa mozaika lubenice poreklom iz Srbije",
pages = "230-213",
number = "3",
volume = "25",
doi = "10.2298/PIF1003213V"
}

Vučurović, A., Bulajić, A., Stanković, I., Ristić, D., Berenji, J.,& Krstić, B.. (2010). Frequency and molecular characterization of watermelon mosaic virus from Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 25(3), 213-230.
https://doi.org/10.2298/PIF1003213V

Vučurović A, Bulajić A, Stanković I, Ristić D, Berenji J, Krstić B. Frequency and molecular characterization of watermelon mosaic virus from Serbia. in Pesticidi i fitomedicina. 2010;25(3):213-230.
doi:10.2298/PIF1003213V .

Vučurović, Ana, Bulajić, Aleksandra, Stanković, Ivana, Ristić, Danijela, Berenji, Janoš, Krstić, Branka, "Frequency and molecular characterization of watermelon mosaic virus from Serbia" in Pesticidi i fitomedicina, 25, no. 3 (2010):213-230,
https://doi.org/10.2298/PIF1003213V . .

TY  - JOUR
AU  - Vučurović, Ana
AU  - Bulajić, Aleksandra
AU  - Djekić, Ivana
AU  - Ristić, Danijela
AU  - Berenji, Janoš
AU  - Krstić, Branka
PY  - 2009
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1953
AB  - Zucchini yellow mosaic virus (ZYMV) has become an important pathogen of cucurbits over the past 20 years. In our country, this virus also causes significant losses, especially in oil pumpkin production. Although ZYMV variability has been extensively studied worldwide by biological and molecular characterization, such information is still limited for the European region. Herein, we present the first analysis of phenotypic variations of ZYMV isolates sampled from oil pumpkin cv. Olinka from different locations in Serbia during 2008. Twelve isolates were obtained by mechanical inoculation on the C. pepo hybrid Ezra F1, and four of them were chosen for further detailed biological study. These investigated isolates exhibited different phenotypical features and thus represented different variants, concerning their host range, and type and severity of symptoms. A previously developed primer pair, ZYMV CPfwd/CPrev, was tested to determine its suitability for detection of Serbian ZYMV isolates. Amplification of Serbian ZYMV isolates of different origin, in single and mixed infection, resulted in one distinctive band. The used primer pair proved to be useful not only for routine molecular detection, but also for further molecular characterization of Serbian ZYMV isolates because it amplifies the hypervariable genomic region of ZYMV. The result obtained in this study is a contribution to investigation of genetic diversity of Serbian ZYMV isolates and their molecular relationship with ZYMV isolates from other parts of the world.
AB  - Poslednjih 20 godina virus žutog mozaika cukinija (Zucchini yellow mosaic virus, ZYMV) se javlja kao značajan patogen biljaka iz familije tikava. Kod nas ovaj virus, takođe, izaziva značajne gubitke, naročito u proizvodnji uljane tikve. Iako je biološka i molekularna varijabilnost ZYMV dosta ispitivana, još uvek nema dovoljno informacija o varijabilnosti evropskih izolata ovog virusa. Ovaj rad predstavlja prvu analizu fenotipske varijabilnosti izolata ZYMV poreklom iz Srbije, koji su tokom 2008. godine sakupljeni iz različitih lokaliteta gajenja uljane tikve sorte Olinka. Mehaničkim inokulacijama C. pepo hibrid Ezra F1, dobijeno je 12 izolata, od kojih su četiri odabrana za dalju biološku karakterizaciju. Na osnovu različitog kruga domaćina, tipa i jačine simptoma na test-biljkama, utvrđena je fenotipska varijabilnost ispitivanih izolata. Par prajmera ZYMV CPfwd/Cprev testiran je radi utvrđivanja pogodnosti za rutinsku detekciju izolata ZYMV poreklom iz Srbije. Na osnovu pojave traka očekivane veličine u gelu, ZYMV je detektovan i u pojedinačnim i mešanim infekcijama u biljkama sa različitih lokaliteta, što dokazuje pogodnost ovih prajmera. Kako ovi prajmeri amplifikuju hipervarijabilni deo genoma virusa, pogodni su ne samo za rutinsku molekularnu detekciju, već i za molekularnu karakterizaciju izolata ZYMV iz Srbije. Rezultati dobijeni u ovom radu omogućiće dalja proučavanja genetičke varijabilnosti izolata ZYMV iz Srbije, kao i njihove evolutivne povezanosti sa izolatima iz drugih delova sveta.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Biological variability of zucchini yellow mosaic virus in Serbia
T1  - Biološka varijabilnost virusa žutog mozaika cukinija u Srbiji
EP  - 280
IS  - 4
SP  - 271
VL  - 24
DO  - 10.2298/PIF0904271V
ER  - 

@article{
author = "Vučurović, Ana and Bulajić, Aleksandra and Djekić, Ivana and Ristić, Danijela and Berenji, Janoš and Krstić, Branka",
year = "2009",
abstract = "Zucchini yellow mosaic virus (ZYMV) has become an important pathogen of cucurbits over the past 20 years. In our country, this virus also causes significant losses, especially in oil pumpkin production. Although ZYMV variability has been extensively studied worldwide by biological and molecular characterization, such information is still limited for the European region. Herein, we present the first analysis of phenotypic variations of ZYMV isolates sampled from oil pumpkin cv. Olinka from different locations in Serbia during 2008. Twelve isolates were obtained by mechanical inoculation on the C. pepo hybrid Ezra F1, and four of them were chosen for further detailed biological study. These investigated isolates exhibited different phenotypical features and thus represented different variants, concerning their host range, and type and severity of symptoms. A previously developed primer pair, ZYMV CPfwd/CPrev, was tested to determine its suitability for detection of Serbian ZYMV isolates. Amplification of Serbian ZYMV isolates of different origin, in single and mixed infection, resulted in one distinctive band. The used primer pair proved to be useful not only for routine molecular detection, but also for further molecular characterization of Serbian ZYMV isolates because it amplifies the hypervariable genomic region of ZYMV. The result obtained in this study is a contribution to investigation of genetic diversity of Serbian ZYMV isolates and their molecular relationship with ZYMV isolates from other parts of the world., Poslednjih 20 godina virus žutog mozaika cukinija (Zucchini yellow mosaic virus, ZYMV) se javlja kao značajan patogen biljaka iz familije tikava. Kod nas ovaj virus, takođe, izaziva značajne gubitke, naročito u proizvodnji uljane tikve. Iako je biološka i molekularna varijabilnost ZYMV dosta ispitivana, još uvek nema dovoljno informacija o varijabilnosti evropskih izolata ovog virusa. Ovaj rad predstavlja prvu analizu fenotipske varijabilnosti izolata ZYMV poreklom iz Srbije, koji su tokom 2008. godine sakupljeni iz različitih lokaliteta gajenja uljane tikve sorte Olinka. Mehaničkim inokulacijama C. pepo hibrid Ezra F1, dobijeno je 12 izolata, od kojih su četiri odabrana za dalju biološku karakterizaciju. Na osnovu različitog kruga domaćina, tipa i jačine simptoma na test-biljkama, utvrđena je fenotipska varijabilnost ispitivanih izolata. Par prajmera ZYMV CPfwd/Cprev testiran je radi utvrđivanja pogodnosti za rutinsku detekciju izolata ZYMV poreklom iz Srbije. Na osnovu pojave traka očekivane veličine u gelu, ZYMV je detektovan i u pojedinačnim i mešanim infekcijama u biljkama sa različitih lokaliteta, što dokazuje pogodnost ovih prajmera. Kako ovi prajmeri amplifikuju hipervarijabilni deo genoma virusa, pogodni su ne samo za rutinsku molekularnu detekciju, već i za molekularnu karakterizaciju izolata ZYMV iz Srbije. Rezultati dobijeni u ovom radu omogućiće dalja proučavanja genetičke varijabilnosti izolata ZYMV iz Srbije, kao i njihove evolutivne povezanosti sa izolatima iz drugih delova sveta.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Biological variability of zucchini yellow mosaic virus in Serbia, Biološka varijabilnost virusa žutog mozaika cukinija u Srbiji",
pages = "280-271",
number = "4",
volume = "24",
doi = "10.2298/PIF0904271V"
}

Vučurović, A., Bulajić, A., Djekić, I., Ristić, D., Berenji, J.,& Krstić, B.. (2009). Biological variability of zucchini yellow mosaic virus in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 24(4), 271-280.
https://doi.org/10.2298/PIF0904271V

Vučurović A, Bulajić A, Djekić I, Ristić D, Berenji J, Krstić B. Biological variability of zucchini yellow mosaic virus in Serbia. in Pesticidi i fitomedicina. 2009;24(4):271-280.
doi:10.2298/PIF0904271V .

Vučurović, Ana, Bulajić, Aleksandra, Djekić, Ivana, Ristić, Danijela, Berenji, Janoš, Krstić, Branka, "Biological variability of zucchini yellow mosaic virus in Serbia" in Pesticidi i fitomedicina, 24, no. 4 (2009):271-280,
https://doi.org/10.2298/PIF0904271V . .

TY  - JOUR
AU  - Vučurović, Ana
AU  - Bulajić, Aleksandra
AU  - Djekić, Ivana
AU  - Ristić, Danijela
AU  - Berenji, Janoš
AU  - Krstić, Branka
PY  - 2009
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1952
AB  - Over the past decade, intensive spread of virus infections of oilseed pumpkin has resulted in significant economic losses in pumpkin crop production, which is currently expanding in our country. In 2007 and 2008, a survey for the presence and distribution of oilseed pumpkin viruses was carried out in order to identify viruses responsible for epidemics and incidences of very destructive symptoms on cucurbit leaves and fruits. Monitoring and collecting samples of oil pumpkin, as well as other species such as winter and butternut squash and buffalo and bottle gourd with viral infection symptoms, was conducted in several localities of Vojvodina Province. The collected plant samples were tested by DAS-ELISA using polyclonal antisera specific for the detection of six most economically harmful pumpkin viruses: Cucumber mosaic virus (CMV), Zucchini yellow mosaic virus (ZYMV), Watermelon mosaic virus (WMW), Squash mosaic virus (SqMV), Papaya ringspot virus (PRSV) and Tobacco ringspot virus (TRSV) that are included in A1 quarantine list of harmful organisms in Serbia. Identification of viruses in the collected samples indicated the presence of three viruses, ZYMV, WMV and CMV, in individual and mixed infections. Frequency of the identified viruses varied depending on locality and year of investigations. In 2007, WMV was the most frequent virus (94.2%), while ZYMV was prevalent (98.04%) in 2008. High frequency of ZYMV determined in both years of investigation indicated the need for its rapid and reliable molecular detection. During this investigation, a protocol for ZYMV detection was developed and optimized using specific primers CPfwd/Cprev and commercial kits for total RNA extraction, as well as for RT-PCR. In RT-PCR reaction using these primers, a DNA fragment of approximately 1100 bp, which included coat protein gene, was amplified in the samples of infected pumkin leaves. Although serological methods are still useful for large-scale testing of a great number of samples, this protocol, due to its high sensitivity and specificity, is an important improvement in rapid diagnosis of diseases caused by this virus. In addition, the protocol provides a basis for further characterization of ZYMV isolates originating from Serbia.
AB  - Intenzivno širenje virusa infektivnih za uljanu tikvu (Cucurbita pepo), poslednjih deset godina imalo je za posledicu značajne ekonomske gubitke u proizvodnji ove kulture koja se gaji na sve većim površinama u našoj zemlji. Kako bi se identifikovali virusi, odgovorni za epidemijsku pojavu i ispoljavanje veoma destruktivnih simptoma na lišću i plodovima uljane tikve, tokom 2007. i 2008. sprovedeno je ispitivanje njihove pojave i rasprostranjenosti. Pregled i sakupljanje uzoraka uljane tikve, kao i nekih drugih vrsta tikava, kao što su bundeva, muskatna i bizonska tikva i vrg sa simptomima virusnih zaraza, obavljeno je na više različitih lokaliteta gajenja uljane tikve u Vojvodini. Sakupljeni uzorci testirani su DAS-ELISA metodom primenom poliklonalnih antiseruma specifičnih za detekciju u svetu šest ekonomski najznačajnijih virusa tikava: virusa mozaika krastavca (Cucumber mosaic virus, CMV), virusa žutog mozaika cukinija (Zucchini yellow mosaic virus, ZYMV), virusa mozaika lubenice (Watermelon mosaic virus, WMW), virusa mozaika bundeve (Squash mosaic virus, SqMV), virusa prstenaste pegavosti papaje (Papaya ringspot virus, PRSV) i virusa prstenaste pegavosti duvana (Tobacco ringspot virus, TRSV), koji se nalazi na A1 karantinskoj listi štetnih organizama u Srbiji. Identifikacija virusa u sakupljenim uzorcima ukazala je na prisustvo tri virusa, ZYMV, WMV i CMV, koji su se javili u pojedinačnim ili mešanim infekcijama. Njihova učestalost je po pojedinim godinama i lokalitetima bila različita. Tokom 2007. najčešće je dokazan WMV (94,2%), dok je 2008. godine prevalentan virus bio ZYMV (98,04%). Velika učestalost ZYMV u obe godine ispitivanja ukazala je na potrebu za brzom i pouzdanom molekularnom detekcijom ovog virusa. U toku ovih istraživanja razvijen je i optimizovan protokol za detekciju ZYMV primenom specifičnih prajmera CPfwd/CPrev i komercijalnih kitova za ekstrakciju ukupne RNA i RT-PCR. Korišćenjem ovih prajmera, kojim se amplifikuje deo genoma ZYMV kojim je obuhvaćen i gen za proteinski omotač, umnožen je DNK fragment dužine oko 1100 bp iz lišća zaraženih biljaka. Mada serološke metode i dalje imaju veliku prednost u primeni za masovna testiranja velikog broja uzoraka, razvijeni protokol molekularne detekcije, zbog visoke osetljivosti i specifičnosti predstavlja značajno poboljšanje brze dijagnoze oboljenja koja ovaj virus izaziva. Osim toga, ovaj protokol pruža osnovu za dalju karakterizaciju ZYMV izolata poreklom iz Srbije.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Presence and distribution of oilseed pumpkin viruses and molecular detection of Zucchini yellow mosaic virus
T1  - Prisustvo i rasprostranjenost virusa uljane tikve i molekularna detekcija virusa žutog mozaika cukinija
EP  - 94
IS  - 2
SP  - 85
VL  - 24
DO  - 10.2298/PIF0902085V
ER  - 

@article{
author = "Vučurović, Ana and Bulajić, Aleksandra and Djekić, Ivana and Ristić, Danijela and Berenji, Janoš and Krstić, Branka",
year = "2009",
abstract = "Over the past decade, intensive spread of virus infections of oilseed pumpkin has resulted in significant economic losses in pumpkin crop production, which is currently expanding in our country. In 2007 and 2008, a survey for the presence and distribution of oilseed pumpkin viruses was carried out in order to identify viruses responsible for epidemics and incidences of very destructive symptoms on cucurbit leaves and fruits. Monitoring and collecting samples of oil pumpkin, as well as other species such as winter and butternut squash and buffalo and bottle gourd with viral infection symptoms, was conducted in several localities of Vojvodina Province. The collected plant samples were tested by DAS-ELISA using polyclonal antisera specific for the detection of six most economically harmful pumpkin viruses: Cucumber mosaic virus (CMV), Zucchini yellow mosaic virus (ZYMV), Watermelon mosaic virus (WMW), Squash mosaic virus (SqMV), Papaya ringspot virus (PRSV) and Tobacco ringspot virus (TRSV) that are included in A1 quarantine list of harmful organisms in Serbia. Identification of viruses in the collected samples indicated the presence of three viruses, ZYMV, WMV and CMV, in individual and mixed infections. Frequency of the identified viruses varied depending on locality and year of investigations. In 2007, WMV was the most frequent virus (94.2%), while ZYMV was prevalent (98.04%) in 2008. High frequency of ZYMV determined in both years of investigation indicated the need for its rapid and reliable molecular detection. During this investigation, a protocol for ZYMV detection was developed and optimized using specific primers CPfwd/Cprev and commercial kits for total RNA extraction, as well as for RT-PCR. In RT-PCR reaction using these primers, a DNA fragment of approximately 1100 bp, which included coat protein gene, was amplified in the samples of infected pumkin leaves. Although serological methods are still useful for large-scale testing of a great number of samples, this protocol, due to its high sensitivity and specificity, is an important improvement in rapid diagnosis of diseases caused by this virus. In addition, the protocol provides a basis for further characterization of ZYMV isolates originating from Serbia., Intenzivno širenje virusa infektivnih za uljanu tikvu (Cucurbita pepo), poslednjih deset godina imalo je za posledicu značajne ekonomske gubitke u proizvodnji ove kulture koja se gaji na sve većim površinama u našoj zemlji. Kako bi se identifikovali virusi, odgovorni za epidemijsku pojavu i ispoljavanje veoma destruktivnih simptoma na lišću i plodovima uljane tikve, tokom 2007. i 2008. sprovedeno je ispitivanje njihove pojave i rasprostranjenosti. Pregled i sakupljanje uzoraka uljane tikve, kao i nekih drugih vrsta tikava, kao što su bundeva, muskatna i bizonska tikva i vrg sa simptomima virusnih zaraza, obavljeno je na više različitih lokaliteta gajenja uljane tikve u Vojvodini. Sakupljeni uzorci testirani su DAS-ELISA metodom primenom poliklonalnih antiseruma specifičnih za detekciju u svetu šest ekonomski najznačajnijih virusa tikava: virusa mozaika krastavca (Cucumber mosaic virus, CMV), virusa žutog mozaika cukinija (Zucchini yellow mosaic virus, ZYMV), virusa mozaika lubenice (Watermelon mosaic virus, WMW), virusa mozaika bundeve (Squash mosaic virus, SqMV), virusa prstenaste pegavosti papaje (Papaya ringspot virus, PRSV) i virusa prstenaste pegavosti duvana (Tobacco ringspot virus, TRSV), koji se nalazi na A1 karantinskoj listi štetnih organizama u Srbiji. Identifikacija virusa u sakupljenim uzorcima ukazala je na prisustvo tri virusa, ZYMV, WMV i CMV, koji su se javili u pojedinačnim ili mešanim infekcijama. Njihova učestalost je po pojedinim godinama i lokalitetima bila različita. Tokom 2007. najčešće je dokazan WMV (94,2%), dok je 2008. godine prevalentan virus bio ZYMV (98,04%). Velika učestalost ZYMV u obe godine ispitivanja ukazala je na potrebu za brzom i pouzdanom molekularnom detekcijom ovog virusa. U toku ovih istraživanja razvijen je i optimizovan protokol za detekciju ZYMV primenom specifičnih prajmera CPfwd/CPrev i komercijalnih kitova za ekstrakciju ukupne RNA i RT-PCR. Korišćenjem ovih prajmera, kojim se amplifikuje deo genoma ZYMV kojim je obuhvaćen i gen za proteinski omotač, umnožen je DNK fragment dužine oko 1100 bp iz lišća zaraženih biljaka. Mada serološke metode i dalje imaju veliku prednost u primeni za masovna testiranja velikog broja uzoraka, razvijeni protokol molekularne detekcije, zbog visoke osetljivosti i specifičnosti predstavlja značajno poboljšanje brze dijagnoze oboljenja koja ovaj virus izaziva. Osim toga, ovaj protokol pruža osnovu za dalju karakterizaciju ZYMV izolata poreklom iz Srbije.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Presence and distribution of oilseed pumpkin viruses and molecular detection of Zucchini yellow mosaic virus, Prisustvo i rasprostranjenost virusa uljane tikve i molekularna detekcija virusa žutog mozaika cukinija",
pages = "94-85",
number = "2",
volume = "24",
doi = "10.2298/PIF0902085V"
}

Vučurović, A., Bulajić, A., Djekić, I., Ristić, D., Berenji, J.,& Krstić, B.. (2009). Presence and distribution of oilseed pumpkin viruses and molecular detection of Zucchini yellow mosaic virus. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 24(2), 85-94.
https://doi.org/10.2298/PIF0902085V

Vučurović A, Bulajić A, Djekić I, Ristić D, Berenji J, Krstić B. Presence and distribution of oilseed pumpkin viruses and molecular detection of Zucchini yellow mosaic virus. in Pesticidi i fitomedicina. 2009;24(2):85-94.
doi:10.2298/PIF0902085V .

Vučurović, Ana, Bulajić, Aleksandra, Djekić, Ivana, Ristić, Danijela, Berenji, Janoš, Krstić, Branka, "Presence and distribution of oilseed pumpkin viruses and molecular detection of Zucchini yellow mosaic virus" in Pesticidi i fitomedicina, 24, no. 2 (2009):85-94,
https://doi.org/10.2298/PIF0902085V . .

TY  - JOUR
AU  - Djekić, Ivana
AU  - Bulajić, Aleksandra
AU  - Vučurović, Ana
AU  - Krstić, Branka
AU  - Jović, Jelena
AU  - Krnjajić, Slobodan
AU  - Berenji, Janoš
PY  - 2008
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1779
AB  - Three-year investigation of the presence and distribution of tobacco viruses in Serbia showed that Cucumber mosaic virus (CMV) appeared every year with different frequency. During 2007, in two localities CMV was even prevalent both in single and in mixed infections, comparing to all other economically important tobacco viruses. Due to distinct leaf symptoms and stunting of plants, CMV infection causes great crop losses and leaf quality decrease and for that reason CMV appears to be very important tobacco virus in Serbia. Continuous presence of CMV in tobacco crops and its economically destructive effect implied the necessity for the development of a rapid and reliable protocol for CMV molecular detection which would be applicable in diagnostic laboratories in our country. During this investigation a rapid and simple protocol was optimized and developed for molecular detection of CMV in tobacco leaves, using primers CMVAu1u/CMVAu2d and commercially available kits for total RNA extraction as well as for RT-PCR. In RT-PCR by these primers that flank the CMV capsid protein gene, a DNA fragment of 847 bp was amplified in the samples of infected tobacco leaves. Obtained results show that established molecular detection procedure is suitable for reliable and efficient detection of tobacco CMV isolates originating from Serbia. Although serological methods are still useful for large-scale testing of great number of samples, this protocol is, due to its high sensitivity and specificity, an important improvement in conformation of the results obtained by other methods, in the detection of virus low concentrations and in further characterization of CMV isolates originating from Serbia. .
AB  - Trogodišnjim proučavanjima pojave i rasprostranjenosti virusa duvana u Srbiji utvrđeno je da se virus mozaika krastavca (Cucumber mosaic virus, CMV) javlja svake godine sa različitom učestalošću. Tokom 2007. godine na dva lokaliteta gajenja utvrđena je njegova prevalentnost kako u pojedinačnim tako i u mešanim infekcijama u odnosu na sve ostale ekonomski značajne viruse duvana. Usled promena u vidu izraženih simptoma na lišću i kržljavosti biljaka, CMV značajno utiče na smanjenje prinosa i kvaliteta lišća pa se smatra izuzetno važnim virusom duvana za našu zemlju. Stalno prisustvo CMV u usevu duvana i pripadnost grupi ekonomski štetnih virusa uslovili su potrebu za razvijanjem brzog i pouzdanog protokola za molekularnu detekciju koji bi bio primenljiv u dijagnostičkim laboratorijama u našoj zemlji. U toku ovih istraživanja razvijen je i optimiziran brz i precizan protokol za molekularnu detekciju CMV u lišću duvana primenom specifičnih prajmera CMVAu1u/CMVAu2d i komercijalnih kitova za ekstrakciju totalne RNA i RT-PCR. Korišćenjem ovih prajmera, čije se mesto vezivanja na genomu CMV nalazi na granicama gena za proteinski omotač, umnožen je fragment dužine od 847 bp. Dobijeni rezultati ukazuju da se razvijeni postupak za molekularnu identifikaciju može uspešno koristiti za detekciju CMV izolata iz Srbije u duvanu. Serološki metodi i dalje predstavljaju metod izbora za masovna testiranja velikog broja uzoraka, ali razvijeni protokol molekularne identifikacije predstavlja, zbog svoje izuzetne osetljivosti i specifičnosti, sredstvo za potvrdu rezultata dobijenih drugim metodima, kao i metod za dokazivanje virusa u niskoj koncentraciji i način za karakterizaciju CMV izolata poreklom iz Srbije.
PB  - Naučni institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Bilten za hmelj, sirak i lekovito bilje
T1  - Frequency and molecular detection of cucumber mosaic virus in tobacco crops
T1  - Zastupljenost i molekularna detekcija virusa mozaika krastavca u usevu duvana
EP  - 82
IS  - 81
SP  - 70
VL  - 40
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1779
ER  - 

@article{
author = "Djekić, Ivana and Bulajić, Aleksandra and Vučurović, Ana and Krstić, Branka and Jović, Jelena and Krnjajić, Slobodan and Berenji, Janoš",
year = "2008",
abstract = "Three-year investigation of the presence and distribution of tobacco viruses in Serbia showed that Cucumber mosaic virus (CMV) appeared every year with different frequency. During 2007, in two localities CMV was even prevalent both in single and in mixed infections, comparing to all other economically important tobacco viruses. Due to distinct leaf symptoms and stunting of plants, CMV infection causes great crop losses and leaf quality decrease and for that reason CMV appears to be very important tobacco virus in Serbia. Continuous presence of CMV in tobacco crops and its economically destructive effect implied the necessity for the development of a rapid and reliable protocol for CMV molecular detection which would be applicable in diagnostic laboratories in our country. During this investigation a rapid and simple protocol was optimized and developed for molecular detection of CMV in tobacco leaves, using primers CMVAu1u/CMVAu2d and commercially available kits for total RNA extraction as well as for RT-PCR. In RT-PCR by these primers that flank the CMV capsid protein gene, a DNA fragment of 847 bp was amplified in the samples of infected tobacco leaves. Obtained results show that established molecular detection procedure is suitable for reliable and efficient detection of tobacco CMV isolates originating from Serbia. Although serological methods are still useful for large-scale testing of great number of samples, this protocol is, due to its high sensitivity and specificity, an important improvement in conformation of the results obtained by other methods, in the detection of virus low concentrations and in further characterization of CMV isolates originating from Serbia. ., Trogodišnjim proučavanjima pojave i rasprostranjenosti virusa duvana u Srbiji utvrđeno je da se virus mozaika krastavca (Cucumber mosaic virus, CMV) javlja svake godine sa različitom učestalošću. Tokom 2007. godine na dva lokaliteta gajenja utvrđena je njegova prevalentnost kako u pojedinačnim tako i u mešanim infekcijama u odnosu na sve ostale ekonomski značajne viruse duvana. Usled promena u vidu izraženih simptoma na lišću i kržljavosti biljaka, CMV značajno utiče na smanjenje prinosa i kvaliteta lišća pa se smatra izuzetno važnim virusom duvana za našu zemlju. Stalno prisustvo CMV u usevu duvana i pripadnost grupi ekonomski štetnih virusa uslovili su potrebu za razvijanjem brzog i pouzdanog protokola za molekularnu detekciju koji bi bio primenljiv u dijagnostičkim laboratorijama u našoj zemlji. U toku ovih istraživanja razvijen je i optimiziran brz i precizan protokol za molekularnu detekciju CMV u lišću duvana primenom specifičnih prajmera CMVAu1u/CMVAu2d i komercijalnih kitova za ekstrakciju totalne RNA i RT-PCR. Korišćenjem ovih prajmera, čije se mesto vezivanja na genomu CMV nalazi na granicama gena za proteinski omotač, umnožen je fragment dužine od 847 bp. Dobijeni rezultati ukazuju da se razvijeni postupak za molekularnu identifikaciju može uspešno koristiti za detekciju CMV izolata iz Srbije u duvanu. Serološki metodi i dalje predstavljaju metod izbora za masovna testiranja velikog broja uzoraka, ali razvijeni protokol molekularne identifikacije predstavlja, zbog svoje izuzetne osetljivosti i specifičnosti, sredstvo za potvrdu rezultata dobijenih drugim metodima, kao i metod za dokazivanje virusa u niskoj koncentraciji i način za karakterizaciju CMV izolata poreklom iz Srbije.",
publisher = "Naučni institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Bilten za hmelj, sirak i lekovito bilje",
title = "Frequency and molecular detection of cucumber mosaic virus in tobacco crops, Zastupljenost i molekularna detekcija virusa mozaika krastavca u usevu duvana",
pages = "82-70",
number = "81",
volume = "40",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1779"
}

Djekić, I., Bulajić, A., Vučurović, A., Krstić, B., Jović, J., Krnjajić, S.,& Berenji, J.. (2008). Frequency and molecular detection of cucumber mosaic virus in tobacco crops. in Bilten za hmelj, sirak i lekovito bilje
Naučni institut za ratarstvo i povrtarstvo, Novi Sad., 40(81), 70-82.
https://hdl.handle.net/21.15107/rcub_agrospace_1779

Djekić I, Bulajić A, Vučurović A, Krstić B, Jović J, Krnjajić S, Berenji J. Frequency and molecular detection of cucumber mosaic virus in tobacco crops. in Bilten za hmelj, sirak i lekovito bilje. 2008;40(81):70-82.
https://hdl.handle.net/21.15107/rcub_agrospace_1779 .

Djekić, Ivana, Bulajić, Aleksandra, Vučurović, Ana, Krstić, Branka, Jović, Jelena, Krnjajić, Slobodan, Berenji, Janoš, "Frequency and molecular detection of cucumber mosaic virus in tobacco crops" in Bilten za hmelj, sirak i lekovito bilje, 40, no. 81 (2008):70-82,
https://hdl.handle.net/21.15107/rcub_agrospace_1779 .

TY  - JOUR
AU  - Krstić, Branka
AU  - Bulajić, Aleksandra
AU  - Djekić, Ivana
AU  - Berenji, Janoš
PY  - 2008
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1668
AB  - Tomato spotted wilt virus (TSWV) has one of the largest host ranges among plant viruses and is widespread in all climates. TSWV is responsible for numerous epidemics in many parts of the world in different crops, mainly vegetables, tobacco and ornamentals. Its highly polyphagous nature, effectiveness of virus transmission by the trips as its vectors, rapidity with which new variants arise, as well as difficulties in controlling the vectors make TSWV one of the most dangerous plant viruses. The ability of this virus to cause such severe losses on a broad range of crops, as well as its intriguing biological and molecular characteristics place TSWV amongst the most extensively studied plant viruses in the world at present. This paper provides a general overview of TSWV, encompassing all the major aspects of its biology and current knowledge on host range, symptomatology, molecular biology, vector relationship, control and diagnosis.
AB  - Smatra se da virus bronzavosti paradajza (Tomato spotted wilt virus, TSWV) ima najširi krug domaćina i da je jedan od najrasprostranjenijih biljnih virusa. Ovaj virus je odgovoran za brojne epidemije na različitim usevima u mnogim delovima sveta, a najčešće na povrću, duvanu i ukrasnim biljkama. Visoko polifagna priroda virusa, efikasnost prenošenja vektorima - tripsima, brzina kojom se stvaraju nove varijante virusa, kao i teškoće u kontroli vektora, čine TSWV jednim od najopasnijih biljnih virusa. Zbog ekonomske važnosti na širokom krugu domaćina, kao i interesantnih bioloških i molekularnih karakteristika, ovo je danas jedan od najproučavanijih biljnih virusa. Ovaj pregledni rad o virusu bronazavosti paradajza bliže objašnjava sve aspekte njegove biologije i sadrži savremene podatke o nekim njegovim osobinama koje se odnose na niz domaćina, simptomatologiju, molekularnu biologiju, odnos sa vektorom, kontrolu i dijagnozu oboljenja koje prouzrokuje.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Tomato spotted wilt virus: One of the most destructive plant viruses
T1  - Virus bronzavosti paradajza - jedan od najdestruktivnijih biljnih virusa
EP  - 166
IS  - 3
SP  - 153
VL  - 23
DO  - 10.2298/PIF0803153K
ER  - 

@article{
author = "Krstić, Branka and Bulajić, Aleksandra and Djekić, Ivana and Berenji, Janoš",
year = "2008",
abstract = "Tomato spotted wilt virus (TSWV) has one of the largest host ranges among plant viruses and is widespread in all climates. TSWV is responsible for numerous epidemics in many parts of the world in different crops, mainly vegetables, tobacco and ornamentals. Its highly polyphagous nature, effectiveness of virus transmission by the trips as its vectors, rapidity with which new variants arise, as well as difficulties in controlling the vectors make TSWV one of the most dangerous plant viruses. The ability of this virus to cause such severe losses on a broad range of crops, as well as its intriguing biological and molecular characteristics place TSWV amongst the most extensively studied plant viruses in the world at present. This paper provides a general overview of TSWV, encompassing all the major aspects of its biology and current knowledge on host range, symptomatology, molecular biology, vector relationship, control and diagnosis., Smatra se da virus bronzavosti paradajza (Tomato spotted wilt virus, TSWV) ima najširi krug domaćina i da je jedan od najrasprostranjenijih biljnih virusa. Ovaj virus je odgovoran za brojne epidemije na različitim usevima u mnogim delovima sveta, a najčešće na povrću, duvanu i ukrasnim biljkama. Visoko polifagna priroda virusa, efikasnost prenošenja vektorima - tripsima, brzina kojom se stvaraju nove varijante virusa, kao i teškoće u kontroli vektora, čine TSWV jednim od najopasnijih biljnih virusa. Zbog ekonomske važnosti na širokom krugu domaćina, kao i interesantnih bioloških i molekularnih karakteristika, ovo je danas jedan od najproučavanijih biljnih virusa. Ovaj pregledni rad o virusu bronazavosti paradajza bliže objašnjava sve aspekte njegove biologije i sadrži savremene podatke o nekim njegovim osobinama koje se odnose na niz domaćina, simptomatologiju, molekularnu biologiju, odnos sa vektorom, kontrolu i dijagnozu oboljenja koje prouzrokuje.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Tomato spotted wilt virus: One of the most destructive plant viruses, Virus bronzavosti paradajza - jedan od najdestruktivnijih biljnih virusa",
pages = "166-153",
number = "3",
volume = "23",
doi = "10.2298/PIF0803153K"
}

Krstić, B., Bulajić, A., Djekić, I.,& Berenji, J.. (2008). Tomato spotted wilt virus: One of the most destructive plant viruses. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 23(3), 153-166.
https://doi.org/10.2298/PIF0803153K

Krstić B, Bulajić A, Djekić I, Berenji J. Tomato spotted wilt virus: One of the most destructive plant viruses. in Pesticidi i fitomedicina. 2008;23(3):153-166.
doi:10.2298/PIF0803153K .

Krstić, Branka, Bulajić, Aleksandra, Djekić, Ivana, Berenji, Janoš, "Tomato spotted wilt virus: One of the most destructive plant viruses" in Pesticidi i fitomedicina, 23, no. 3 (2008):153-166,
https://doi.org/10.2298/PIF0803153K . .

TY  - JOUR
AU  - Djekić, Ivana
AU  - Bulajić, Aleksandra
AU  - Zindović, Jelena
AU  - Berenji, Janoš
AU  - Pauković, Milena
AU  - Krstić, Branka
PY  - 2007
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1473
AB  - Five viruses: Potato Virus Y (PVY), Tomato Spotted Wilt Virus, Cucumber Mosaic Virus, Tobacco Mosaic Virus and Alfalfa Mosaic Virus, of which PVY was predominant, were detected by serological testing of tobacco samples collected from many localities in Vojvodina in 2006. Viruses are the most important pathogens in tobacco and PVY causes considerable economic damages all over the world. A PVY population comprises several different strain groups, strain subgroups and recombinant strains. Among these, PVYN (necrotic strain) and PVYO (ordinary strain) cause the greatest yield and quality losses in tobacco. Identification of a prevalent strain in a PVY population is the basis of proper tobacco genotype selection for resistance against this significant virus. Typical symptoms caused by PVY were observed by monitoring tobacco crops in our country in 2006. The symptoms occurred as changes in the general plant appearance, as well as necrotic areas on leaves, petiols, stems and flowers. The initial symptoms of veinal necrosis were expanded throughout the leaf, causing reddish-brown (copper) plant color and premature death of lower leaves. Plants with these symptoms occurred in all monitored localities and their frequency was high. In order to understand various epidemiological aspects of the diseases caused by PVY and to prevent its occurrence and spreading in tobacco crops, it is necessary to properly identify this virus in time. Biological, serological and molecular identification of the virus and its prevalent strain was carried out in order to determine tobacco disease ethiology. The results obtained suggest that this prevalent strain of PVY has been spreading progressively in our country in recent years. Although PVYN is widely spread in tobacco crops in Europe, its destructiveness, disease intensity and wide distribution in Serbia were established only in the last two years. PVY necrotic strain was detected mainly in single infections, although it was also present in mixed infections with other tobacco viruses.
AB  - Serološkim testiranjem uzoraka duvana prikupljenih u više lokaliteta u Vojvodini 2006. godine detektovani su Potato Virus Y (PVY), Tomato Spotted Wilt Virus, Cucumber Mosaic Virus, Tobacco Mosaic Virus i Alfalfa Mosaic Virus, pri čemu je PVY bio dominantan. Virusi su najznačajniji prouzrokovači oboljenja duvana, a virus crtičastog mozaika krompira (PVY) izaziva ekonomski značajne štete širom sveta. Populaciju PVY čini više različitih grupa sojeva, podgrupa u okviru određenog soja i sojeva nastalih rekombinacijom. Od opisanih, PVYN (nekrotični soj) i PVYO (obični soj) prouzrokuju najveće gubitke u prinosu i kvalitetu duvana. Poznavanje predominantnog soja u populaciji PVY osnova je pravilne selekcije genotipova duvana otpornih na ovaj značajan virus. Pregledom useva duvana u Srbiji tokom 2006. godine, uočeni su tipični simptomi koje izaziva PVY. Simptomi su se ispoljavali u vidu promena opšteg izgleda biljaka, kao i nekrotičnih promena na lišću, peteljkama, stablu i cvetu. Početni simptomi nekroze nerava su se širili i zahvatali celu lisku, usled čega su biljke dobile crvenkastosmeđu (bakarnu) boju, a donje lišće je prevremeno izumiralo. Biljke sa ovakvim simptomima javljale su se u svim pregledanim lokalitetima, a učestalost pojave bila je veoma visoka. Kako bi se razumeli razni epidemiološki aspekti oboljenja koje izaziva PVY i sprečila njegova pojava i širenje u usevu duvana, neophodna je pravilna i pravovremena identifikacija virusa. U cilju razjašnjavanja etiologije izvršena je biološka, serološka i molekularna identifikacija virusa i njegovog prevalentnog soja koji je očigledno u progresivnom širenju poslednjih godina na duvanu u našoj zemlji. Iako je PVYN široko rasprostranjen na duvanu u Evropi, destruktivnost, visok intenzitet zaraze i široka rasprostranjenost ustanovljeni su u Srbiji tek poslednje dve godine. Nekrotični soj PVY detektovan je uglavnom u pojedinačnim infekcijama, mada je, u manjoj meri, dokazan i u mešanim infekcijama sa drugim virusima duvana.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Identification of Potato Virus Y strains in tobacco crops
T1  - Identifikacija sojeva virusa crtičastog mozaika krompira na duvanu
EP  - 163
IS  - 2
SP  - 155
VL  - 22
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1473
ER  - 

@article{
author = "Djekić, Ivana and Bulajić, Aleksandra and Zindović, Jelena and Berenji, Janoš and Pauković, Milena and Krstić, Branka",
year = "2007",
abstract = "Five viruses: Potato Virus Y (PVY), Tomato Spotted Wilt Virus, Cucumber Mosaic Virus, Tobacco Mosaic Virus and Alfalfa Mosaic Virus, of which PVY was predominant, were detected by serological testing of tobacco samples collected from many localities in Vojvodina in 2006. Viruses are the most important pathogens in tobacco and PVY causes considerable economic damages all over the world. A PVY population comprises several different strain groups, strain subgroups and recombinant strains. Among these, PVYN (necrotic strain) and PVYO (ordinary strain) cause the greatest yield and quality losses in tobacco. Identification of a prevalent strain in a PVY population is the basis of proper tobacco genotype selection for resistance against this significant virus. Typical symptoms caused by PVY were observed by monitoring tobacco crops in our country in 2006. The symptoms occurred as changes in the general plant appearance, as well as necrotic areas on leaves, petiols, stems and flowers. The initial symptoms of veinal necrosis were expanded throughout the leaf, causing reddish-brown (copper) plant color and premature death of lower leaves. Plants with these symptoms occurred in all monitored localities and their frequency was high. In order to understand various epidemiological aspects of the diseases caused by PVY and to prevent its occurrence and spreading in tobacco crops, it is necessary to properly identify this virus in time. Biological, serological and molecular identification of the virus and its prevalent strain was carried out in order to determine tobacco disease ethiology. The results obtained suggest that this prevalent strain of PVY has been spreading progressively in our country in recent years. Although PVYN is widely spread in tobacco crops in Europe, its destructiveness, disease intensity and wide distribution in Serbia were established only in the last two years. PVY necrotic strain was detected mainly in single infections, although it was also present in mixed infections with other tobacco viruses., Serološkim testiranjem uzoraka duvana prikupljenih u više lokaliteta u Vojvodini 2006. godine detektovani su Potato Virus Y (PVY), Tomato Spotted Wilt Virus, Cucumber Mosaic Virus, Tobacco Mosaic Virus i Alfalfa Mosaic Virus, pri čemu je PVY bio dominantan. Virusi su najznačajniji prouzrokovači oboljenja duvana, a virus crtičastog mozaika krompira (PVY) izaziva ekonomski značajne štete širom sveta. Populaciju PVY čini više različitih grupa sojeva, podgrupa u okviru određenog soja i sojeva nastalih rekombinacijom. Od opisanih, PVYN (nekrotični soj) i PVYO (obični soj) prouzrokuju najveće gubitke u prinosu i kvalitetu duvana. Poznavanje predominantnog soja u populaciji PVY osnova je pravilne selekcije genotipova duvana otpornih na ovaj značajan virus. Pregledom useva duvana u Srbiji tokom 2006. godine, uočeni su tipični simptomi koje izaziva PVY. Simptomi su se ispoljavali u vidu promena opšteg izgleda biljaka, kao i nekrotičnih promena na lišću, peteljkama, stablu i cvetu. Početni simptomi nekroze nerava su se širili i zahvatali celu lisku, usled čega su biljke dobile crvenkastosmeđu (bakarnu) boju, a donje lišće je prevremeno izumiralo. Biljke sa ovakvim simptomima javljale su se u svim pregledanim lokalitetima, a učestalost pojave bila je veoma visoka. Kako bi se razumeli razni epidemiološki aspekti oboljenja koje izaziva PVY i sprečila njegova pojava i širenje u usevu duvana, neophodna je pravilna i pravovremena identifikacija virusa. U cilju razjašnjavanja etiologije izvršena je biološka, serološka i molekularna identifikacija virusa i njegovog prevalentnog soja koji je očigledno u progresivnom širenju poslednjih godina na duvanu u našoj zemlji. Iako je PVYN široko rasprostranjen na duvanu u Evropi, destruktivnost, visok intenzitet zaraze i široka rasprostranjenost ustanovljeni su u Srbiji tek poslednje dve godine. Nekrotični soj PVY detektovan je uglavnom u pojedinačnim infekcijama, mada je, u manjoj meri, dokazan i u mešanim infekcijama sa drugim virusima duvana.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Identification of Potato Virus Y strains in tobacco crops, Identifikacija sojeva virusa crtičastog mozaika krompira na duvanu",
pages = "163-155",
number = "2",
volume = "22",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1473"
}

Djekić, I., Bulajić, A., Zindović, J., Berenji, J., Pauković, M.,& Krstić, B.. (2007). Identification of Potato Virus Y strains in tobacco crops. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 22(2), 155-163.
https://hdl.handle.net/21.15107/rcub_agrospace_1473

Djekić I, Bulajić A, Zindović J, Berenji J, Pauković M, Krstić B. Identification of Potato Virus Y strains in tobacco crops. in Pesticidi i fitomedicina. 2007;22(2):155-163.
https://hdl.handle.net/21.15107/rcub_agrospace_1473 .

Djekić, Ivana, Bulajić, Aleksandra, Zindović, Jelena, Berenji, Janoš, Pauković, Milena, Krstić, Branka, "Identification of Potato Virus Y strains in tobacco crops" in Pesticidi i fitomedicina, 22, no. 2 (2007):155-163,
https://hdl.handle.net/21.15107/rcub_agrospace_1473 .

TY  - JOUR
AU  - Bulajić, Aleksandra
AU  - Djekić, Ivana
AU  - Berenji, Janoš
AU  - Pauković, Milena
AU  - Antonijević, Dragutin
AU  - Krstić, Branka
PY  - 2007
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1533
AB  - Potato virus Y (PVY) was first reported in 1930s, and now it is present in all potato, tobacco, pepper and tomato growing areas. According to the recent investigations of its presence and distribution, PVY is considered to be the most economically important tobacco virus in our country. Numerous aphid species can transmit this virus in a nonpersistant manner. Although the appearance and severity of the symptoms depend on virus strain as well as plant host cultivar, damage is always significant, especially in the case of early infections. In addition to the direct yield decrease, PVY causes changes in tobacco leaves quality. The most important measures of PVY control are: spatial isolation of tobacco and potato crops, control of aphid population in order to reduce the disease extent and the growing of resistant tobacco varieties when ever available.
AB  - Prvi podaci o virusu crtičastog mozaika krompira (Potato virus Y, PVY) potiču iz 30-ih godina prošlog veka, a danas je poznato da je prisutan u svim područjima gajenja krompira, duvana, paprike i paradajza. Ispitivanje pojave i rasprostranjenosti virusa duvana poslednjih godina su pokazala da je PVY ekonomski najznačajniji virus duvana u našoj zemlji. PVY prenose brojne vrste biljnih vaši na neperzistentan način. Iako izgled i jačina simptoma zavise od soja virusa i sorte biljke domaćina štete su uvek značajne naročito u slučaju ranih zaraza. Pored direktnog smanjenja prinosa PVY dovodi do promene kvaliteta duvanskog lišća. Najvažnije mere kontrole PVY su: gajenje duvana i krompira na prostornoj udaljenosti, suzbijanje lisnih vaši radi smanjenja obima zaraze i korišćenje otpornih sorti duvana.
PB  - Naučni institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Bilten za hmelj, sirak i lekovito bilje
T1  - Potato virus Y in tobacco crops
T1  - Virus crtičastog mozaika krompira na duvanu
EP  - 62
IS  - 80
SP  - 53
VL  - 39
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1533
ER  - 

@article{
author = "Bulajić, Aleksandra and Djekić, Ivana and Berenji, Janoš and Pauković, Milena and Antonijević, Dragutin and Krstić, Branka",
year = "2007",
abstract = "Potato virus Y (PVY) was first reported in 1930s, and now it is present in all potato, tobacco, pepper and tomato growing areas. According to the recent investigations of its presence and distribution, PVY is considered to be the most economically important tobacco virus in our country. Numerous aphid species can transmit this virus in a nonpersistant manner. Although the appearance and severity of the symptoms depend on virus strain as well as plant host cultivar, damage is always significant, especially in the case of early infections. In addition to the direct yield decrease, PVY causes changes in tobacco leaves quality. The most important measures of PVY control are: spatial isolation of tobacco and potato crops, control of aphid population in order to reduce the disease extent and the growing of resistant tobacco varieties when ever available., Prvi podaci o virusu crtičastog mozaika krompira (Potato virus Y, PVY) potiču iz 30-ih godina prošlog veka, a danas je poznato da je prisutan u svim područjima gajenja krompira, duvana, paprike i paradajza. Ispitivanje pojave i rasprostranjenosti virusa duvana poslednjih godina su pokazala da je PVY ekonomski najznačajniji virus duvana u našoj zemlji. PVY prenose brojne vrste biljnih vaši na neperzistentan način. Iako izgled i jačina simptoma zavise od soja virusa i sorte biljke domaćina štete su uvek značajne naročito u slučaju ranih zaraza. Pored direktnog smanjenja prinosa PVY dovodi do promene kvaliteta duvanskog lišća. Najvažnije mere kontrole PVY su: gajenje duvana i krompira na prostornoj udaljenosti, suzbijanje lisnih vaši radi smanjenja obima zaraze i korišćenje otpornih sorti duvana.",
publisher = "Naučni institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Bilten za hmelj, sirak i lekovito bilje",
title = "Potato virus Y in tobacco crops, Virus crtičastog mozaika krompira na duvanu",
pages = "62-53",
number = "80",
volume = "39",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1533"
}

Bulajić, A., Djekić, I., Berenji, J., Pauković, M., Antonijević, D.,& Krstić, B.. (2007). Potato virus Y in tobacco crops. in Bilten za hmelj, sirak i lekovito bilje
Naučni institut za ratarstvo i povrtarstvo, Novi Sad., 39(80), 53-62.
https://hdl.handle.net/21.15107/rcub_agrospace_1533

Bulajić A, Djekić I, Berenji J, Pauković M, Antonijević D, Krstić B. Potato virus Y in tobacco crops. in Bilten za hmelj, sirak i lekovito bilje. 2007;39(80):53-62.
https://hdl.handle.net/21.15107/rcub_agrospace_1533 .

Bulajić, Aleksandra, Djekić, Ivana, Berenji, Janoš, Pauković, Milena, Antonijević, Dragutin, Krstić, Branka, "Potato virus Y in tobacco crops" in Bilten za hmelj, sirak i lekovito bilje, 39, no. 80 (2007):53-62,
https://hdl.handle.net/21.15107/rcub_agrospace_1533 .

TY  - JOUR
AU  - Krstić, Branka
AU  - Bulajić, Aleksandra
AU  - Dukić, Nataša
AU  - Duduk, Bojan
AU  - Berenji, Janoš
PY  - 2006
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1307
AB  - Many features of Tomato spotted wilt virus make this virus unique among plant infecting viruses. This virus is considered to be the most widespread virus in the world and that fact is attributed to the rapid expansion of its the most efficient vector Frankliniella occidentalis (Western flower thrips). Tomato spotted wilt virus is extremely polyphagous with, probably, the widest host range of all plant viruses. It causes very destructive diseases of many economically important plants, especially in tobacco, tomato, pepper and ornamentals. It is also responsible for numerous epidemics in tobacco crops in different regions of the world. Since the control of the tomato spotted wilt virus is very complicated, it makes its significance even greater. Considerable efforts have been made in developing resistance or tolerance to Tomato spotted wilt virus infections in certain tobacco cultivars. The results obtained so far in breeding for virus resistance have been disappointed because of the lack of suitable natural forms of resistance, which if there is, is in most cases polygenic and based on the complex interaction among the virus, vector and plant. That is why there is a need for new genetically engineered forms of host plant resistance. However, the extreme variability of Tomato spotted wilt virus is the main cause of breaking the natural or engineered resistance of plants. Also, several biological characteristics of the thrips and rapid development of its resistance to insecticides make its control extremely difficult. It has been discovered recently that an early treatment of tobacco plants with the combination of two chemicals (an insecticide - imidacloprid and a plant defense activator - acibenzolar-S-methyl) could reduce or eliminate the symptoms of Tomato spotted wilt virus on tobacco. This, for the first time, gives the possibility of true control. Good knowledge of the biology and epidemiology of both the virus and its vector is essential for its successful control. An adequate control of Tomato spotted wilt virus can be achieved by integrated pest management including a combination of certain physical, cultural and chemical measures.
AB  - Virus bronzavosti paradajza (Tomato spotted wilt virus) je, po mnogim svojim osobinama, jedinstven u odnosu na druge biljne viruse. Smatra se da je najrasprostranjeniji virus u svetu, zahvaljujući brzoj ekspanziji svog najefikasnijeg vektora - kalifornijskog tripsa, Frankliniella occidentalis. Virus bronzavosti paradajza je izrazito polifagan sa, verovatno, najširim krugom domaćina među svim biljnim virusima. Prouzrokuje veoma destruktivna oboljenja mnogih ekonomski važnih gajenih biljaka, a pre svega duvana, paradajza, paprike i ukrasnih biljaka. Na duvanu je izazvao brojne epidemije u različitim delovima sveta. Kontrola oboljenja koje ovaj virus izaziva na duvanu veoma je složena, što čini značaj ovog virusa još većim. Učinjeni su značajni napori da se dobiju rezistentni ili tolerantni kultivari duvana zaraze ovim virusom, ali su rezultati selekcije na otpornost razočaravajući. Razlog leži u nedostatku odgovarajuće forme prirodne otpornosti koja je, ako je uopšte ima, poligenetske prirode i zasniva se na kompleksnoj interakciji virusa, vektora i biljke. Zbog toga se ukazala potreba za iznalaženjem novih vidova otpornosti putem genetičkog inženjerstva. Međutim, teškoće u kontroli pričinjava i velika varijabilnost virusa, što uslovljava brzo slamanje otpornosti biljaka, bilo da je dobijena prirodnim putem ili genetičkim inženjerstvom. Takođe, neke biološke karakteristike tripsa i brzo razvijanje rezistentnosti prema insekticidima, čine kontrolu virusa izrazito teškom. Nedavno je otkriveno da dva jedinjenja, insekticid-imidacloprid i aktivator otpornosti biljaka - acibenzolar-S-methyl, ako se primene zajedno u ranim fazama razvoja duvana, mogu da smanje ili potpuno eliminišu simptome koje ovaj virus izaziva na duvanu. Ovo otkriće pruža mogućnost prave kontrole po prvi put. Uspešna kontrola oboljenja koje ovaj virus izaziva na duvanu podrazumeva dobro razumevanje biologije i epidemiologije i virusa i tripsa kao vektora. Sprovođenjem integralnog programa zaštite kombinacijom određenih fizičkih, agrotehničkih i hemijskih mera može se postići odgovarajuća kontrola virusa bronzavosti paradajza na duvanu.
PB  - Naučni institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Bilten za hmelj, sirak i lekovito bilje
T1  - Integrated pest management of tomato spotted wilt virus in tobacco
T1  - Integralna zaštita duvana od virusa bronzavosti paradajza
EP  - 60
IS  - 79
SP  - 49
VL  - 38
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1307
ER  - 

@article{
author = "Krstić, Branka and Bulajić, Aleksandra and Dukić, Nataša and Duduk, Bojan and Berenji, Janoš",
year = "2006",
abstract = "Many features of Tomato spotted wilt virus make this virus unique among plant infecting viruses. This virus is considered to be the most widespread virus in the world and that fact is attributed to the rapid expansion of its the most efficient vector Frankliniella occidentalis (Western flower thrips). Tomato spotted wilt virus is extremely polyphagous with, probably, the widest host range of all plant viruses. It causes very destructive diseases of many economically important plants, especially in tobacco, tomato, pepper and ornamentals. It is also responsible for numerous epidemics in tobacco crops in different regions of the world. Since the control of the tomato spotted wilt virus is very complicated, it makes its significance even greater. Considerable efforts have been made in developing resistance or tolerance to Tomato spotted wilt virus infections in certain tobacco cultivars. The results obtained so far in breeding for virus resistance have been disappointed because of the lack of suitable natural forms of resistance, which if there is, is in most cases polygenic and based on the complex interaction among the virus, vector and plant. That is why there is a need for new genetically engineered forms of host plant resistance. However, the extreme variability of Tomato spotted wilt virus is the main cause of breaking the natural or engineered resistance of plants. Also, several biological characteristics of the thrips and rapid development of its resistance to insecticides make its control extremely difficult. It has been discovered recently that an early treatment of tobacco plants with the combination of two chemicals (an insecticide - imidacloprid and a plant defense activator - acibenzolar-S-methyl) could reduce or eliminate the symptoms of Tomato spotted wilt virus on tobacco. This, for the first time, gives the possibility of true control. Good knowledge of the biology and epidemiology of both the virus and its vector is essential for its successful control. An adequate control of Tomato spotted wilt virus can be achieved by integrated pest management including a combination of certain physical, cultural and chemical measures., Virus bronzavosti paradajza (Tomato spotted wilt virus) je, po mnogim svojim osobinama, jedinstven u odnosu na druge biljne viruse. Smatra se da je najrasprostranjeniji virus u svetu, zahvaljujući brzoj ekspanziji svog najefikasnijeg vektora - kalifornijskog tripsa, Frankliniella occidentalis. Virus bronzavosti paradajza je izrazito polifagan sa, verovatno, najširim krugom domaćina među svim biljnim virusima. Prouzrokuje veoma destruktivna oboljenja mnogih ekonomski važnih gajenih biljaka, a pre svega duvana, paradajza, paprike i ukrasnih biljaka. Na duvanu je izazvao brojne epidemije u različitim delovima sveta. Kontrola oboljenja koje ovaj virus izaziva na duvanu veoma je složena, što čini značaj ovog virusa još većim. Učinjeni su značajni napori da se dobiju rezistentni ili tolerantni kultivari duvana zaraze ovim virusom, ali su rezultati selekcije na otpornost razočaravajući. Razlog leži u nedostatku odgovarajuće forme prirodne otpornosti koja je, ako je uopšte ima, poligenetske prirode i zasniva se na kompleksnoj interakciji virusa, vektora i biljke. Zbog toga se ukazala potreba za iznalaženjem novih vidova otpornosti putem genetičkog inženjerstva. Međutim, teškoće u kontroli pričinjava i velika varijabilnost virusa, što uslovljava brzo slamanje otpornosti biljaka, bilo da je dobijena prirodnim putem ili genetičkim inženjerstvom. Takođe, neke biološke karakteristike tripsa i brzo razvijanje rezistentnosti prema insekticidima, čine kontrolu virusa izrazito teškom. Nedavno je otkriveno da dva jedinjenja, insekticid-imidacloprid i aktivator otpornosti biljaka - acibenzolar-S-methyl, ako se primene zajedno u ranim fazama razvoja duvana, mogu da smanje ili potpuno eliminišu simptome koje ovaj virus izaziva na duvanu. Ovo otkriće pruža mogućnost prave kontrole po prvi put. Uspešna kontrola oboljenja koje ovaj virus izaziva na duvanu podrazumeva dobro razumevanje biologije i epidemiologije i virusa i tripsa kao vektora. Sprovođenjem integralnog programa zaštite kombinacijom određenih fizičkih, agrotehničkih i hemijskih mera može se postići odgovarajuća kontrola virusa bronzavosti paradajza na duvanu.",
publisher = "Naučni institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Bilten za hmelj, sirak i lekovito bilje",
title = "Integrated pest management of tomato spotted wilt virus in tobacco, Integralna zaštita duvana od virusa bronzavosti paradajza",
pages = "60-49",
number = "79",
volume = "38",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1307"
}

Krstić, B., Bulajić, A., Dukić, N., Duduk, B.,& Berenji, J.. (2006). Integrated pest management of tomato spotted wilt virus in tobacco. in Bilten za hmelj, sirak i lekovito bilje
Naučni institut za ratarstvo i povrtarstvo, Novi Sad., 38(79), 49-60.
https://hdl.handle.net/21.15107/rcub_agrospace_1307

Krstić B, Bulajić A, Dukić N, Duduk B, Berenji J. Integrated pest management of tomato spotted wilt virus in tobacco. in Bilten za hmelj, sirak i lekovito bilje. 2006;38(79):49-60.
https://hdl.handle.net/21.15107/rcub_agrospace_1307 .

Krstić, Branka, Bulajić, Aleksandra, Dukić, Nataša, Duduk, Bojan, Berenji, Janoš, "Integrated pest management of tomato spotted wilt virus in tobacco" in Bilten za hmelj, sirak i lekovito bilje, 38, no. 79 (2006):49-60,
https://hdl.handle.net/21.15107/rcub_agrospace_1307 .

TY  - JOUR
AU  - Dukić, Nataša
AU  - Bulajić, Aleksandra
AU  - Berenji, Janoš
AU  - Djekić, Ivana
AU  - Duduk, Bojan
AU  - Krstić, Branka
PY  - 2006
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1184
AB  - Infection with a large number of plant viruses could imperil tobacco yield and quality. Tobacco is a natural host for more than 20 viruses, among which the most important and economically harmful are tobacco mosaic virus (TMV), tomato spotted wilt virus (TSWV), cucumber mosaic virus (CMV), potato virus Y (PVY), alfalfa mosaic virus (AMV), tobacco ring-spot virus (TRSV), tobacco each virus (TEV) and tobacco vein mottling virus (TVMV). The occurrence and distribution of tobacco viruses were investigated for 4 years (20022005). During this period many different tobacco growing localities in Vojvodina and central Serbia were monitored and samples showing virus symptoms were collected. The collected samples were tested by DAS ELISA using polyclonal antisera, specific for the detection of PVY, TSWV, TMV, CMV, AMV and TRSV. The results obtained for the tobacco virus distribution during these four years of investigation proved the presence of four economically important viruses in our country, whose frequencies varied from year to year. In 2002, 2003 and 2004, the most frequent was TSWV (86.84%; 79% and 49.56%, respectively), while in 2005 PVY was prevalent (56.16%). All viruses detected in the samples tested were present in single or mixed infections. A correlation was established between the field symptoms on tobacco and the virus causal agents. The results obtained showed that TSWV and PVY were the most important tobacco viruses in our country, so that further research of tobacco virus diseases should be directed towards their more detailed characterization.
AB  - Prinos i kvalitet duvana mogu da budu ugroženi usled zaraze velikim brojem fitopatogenih virusa. Duvan je prirodni domaćin preko 20 virusa od kojih su najznačajniji i ekonomski najštetniji virus mozaika duvana (Tobacco mosaic virus, TMV), virus bronzavosti paradajza (Tomato spotted wilt virus, TSWV), virus mozaika krastavca (Cucumber mosaic virus, CMV), virus crtičastog mozaika krompira (Potato virus Y, PVY), virus mozaika lucerke (Alfalfa mosaic virus, AMV), virus prstenaste pegavosti duvana (Tobacco ringspot virus, TRSV), virus graviranosti duvana (Tobacco each virus, TEV) i virus šarenila nerava duvana (Tobacco vein mottling virus, TVMV). Prisustvo i rasprostranjenost virusnih zaraza duvana su ispitivani tokom četiri godine, od 2002. do 2005. Pregled i sakupljanje uzoraka sa simptomima obavljeno je u više različitih lokaliteta gajenja duvana u Vojvodini i centralnoj Srbiji. Sakupljeni uzorci su testirani DASELISA metodom na prisustvo PVY, TSWV, TMV, CMV, AMV i TRSV. Dobijeni rezultati ispitivanja ukazuju na prisustvo četiri ekonomski značajna virusa duvana u našoj zemlji i to: TSWV, PVY, TMV i CMV, čija je učestalost po pojedinim godinama i lokalitetima bila različita. Tokom 2002, 2003. i 2004. godine najčešće detektovan virus bio je TSWV (86,84%; 70%, odnosno 49,56%), dok je 2005. godine prevalentan virus bio PVY (56,16%). Svi virusi čije je prisustvo utvrđeno u ispitivanim uzorcima nalazili su se u pojedinačnim ili mešanim infekcijama. Bez obzira na izraženu varijabilnost u ispoljenim simptomima virusnih zaraza na duvanu, ustanovljena je zavisnost tipa ispoljenih simptoma od virusa prouzrokovača. Sprovedena ispitivanja pokazala su da su TSWV i PVY najznačajniji virusi duvana u našoj zemlji, tako da buduća ispitivanja viroza duvana treba da budu usmerena ka detaljnijoj karakterizaciji ovih ekonomski štetnih virusa.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Presence and distribution of tobacco viruses in Serbia
T1  - Prisustvo i rasprostranjenost virusa duvana u Srbiji
EP  - 214
IS  - 3
SP  - 205
VL  - 21
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1184
ER  - 

@article{
author = "Dukić, Nataša and Bulajić, Aleksandra and Berenji, Janoš and Djekić, Ivana and Duduk, Bojan and Krstić, Branka",
year = "2006",
abstract = "Infection with a large number of plant viruses could imperil tobacco yield and quality. Tobacco is a natural host for more than 20 viruses, among which the most important and economically harmful are tobacco mosaic virus (TMV), tomato spotted wilt virus (TSWV), cucumber mosaic virus (CMV), potato virus Y (PVY), alfalfa mosaic virus (AMV), tobacco ring-spot virus (TRSV), tobacco each virus (TEV) and tobacco vein mottling virus (TVMV). The occurrence and distribution of tobacco viruses were investigated for 4 years (20022005). During this period many different tobacco growing localities in Vojvodina and central Serbia were monitored and samples showing virus symptoms were collected. The collected samples were tested by DAS ELISA using polyclonal antisera, specific for the detection of PVY, TSWV, TMV, CMV, AMV and TRSV. The results obtained for the tobacco virus distribution during these four years of investigation proved the presence of four economically important viruses in our country, whose frequencies varied from year to year. In 2002, 2003 and 2004, the most frequent was TSWV (86.84%; 79% and 49.56%, respectively), while in 2005 PVY was prevalent (56.16%). All viruses detected in the samples tested were present in single or mixed infections. A correlation was established between the field symptoms on tobacco and the virus causal agents. The results obtained showed that TSWV and PVY were the most important tobacco viruses in our country, so that further research of tobacco virus diseases should be directed towards their more detailed characterization., Prinos i kvalitet duvana mogu da budu ugroženi usled zaraze velikim brojem fitopatogenih virusa. Duvan je prirodni domaćin preko 20 virusa od kojih su najznačajniji i ekonomski najštetniji virus mozaika duvana (Tobacco mosaic virus, TMV), virus bronzavosti paradajza (Tomato spotted wilt virus, TSWV), virus mozaika krastavca (Cucumber mosaic virus, CMV), virus crtičastog mozaika krompira (Potato virus Y, PVY), virus mozaika lucerke (Alfalfa mosaic virus, AMV), virus prstenaste pegavosti duvana (Tobacco ringspot virus, TRSV), virus graviranosti duvana (Tobacco each virus, TEV) i virus šarenila nerava duvana (Tobacco vein mottling virus, TVMV). Prisustvo i rasprostranjenost virusnih zaraza duvana su ispitivani tokom četiri godine, od 2002. do 2005. Pregled i sakupljanje uzoraka sa simptomima obavljeno je u više različitih lokaliteta gajenja duvana u Vojvodini i centralnoj Srbiji. Sakupljeni uzorci su testirani DASELISA metodom na prisustvo PVY, TSWV, TMV, CMV, AMV i TRSV. Dobijeni rezultati ispitivanja ukazuju na prisustvo četiri ekonomski značajna virusa duvana u našoj zemlji i to: TSWV, PVY, TMV i CMV, čija je učestalost po pojedinim godinama i lokalitetima bila različita. Tokom 2002, 2003. i 2004. godine najčešće detektovan virus bio je TSWV (86,84%; 70%, odnosno 49,56%), dok je 2005. godine prevalentan virus bio PVY (56,16%). Svi virusi čije je prisustvo utvrđeno u ispitivanim uzorcima nalazili su se u pojedinačnim ili mešanim infekcijama. Bez obzira na izraženu varijabilnost u ispoljenim simptomima virusnih zaraza na duvanu, ustanovljena je zavisnost tipa ispoljenih simptoma od virusa prouzrokovača. Sprovedena ispitivanja pokazala su da su TSWV i PVY najznačajniji virusi duvana u našoj zemlji, tako da buduća ispitivanja viroza duvana treba da budu usmerena ka detaljnijoj karakterizaciji ovih ekonomski štetnih virusa.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Presence and distribution of tobacco viruses in Serbia, Prisustvo i rasprostranjenost virusa duvana u Srbiji",
pages = "214-205",
number = "3",
volume = "21",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1184"
}

Dukić, N., Bulajić, A., Berenji, J., Djekić, I., Duduk, B.,& Krstić, B.. (2006). Presence and distribution of tobacco viruses in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 21(3), 205-214.
https://hdl.handle.net/21.15107/rcub_agrospace_1184

Dukić N, Bulajić A, Berenji J, Djekić I, Duduk B, Krstić B. Presence and distribution of tobacco viruses in Serbia. in Pesticidi i fitomedicina. 2006;21(3):205-214.
https://hdl.handle.net/21.15107/rcub_agrospace_1184 .

Dukić, Nataša, Bulajić, Aleksandra, Berenji, Janoš, Djekić, Ivana, Duduk, Bojan, Krstić, Branka, "Presence and distribution of tobacco viruses in Serbia" in Pesticidi i fitomedicina, 21, no. 3 (2006):205-214,
https://hdl.handle.net/21.15107/rcub_agrospace_1184 .

TY  - JOUR
AU  - Krstić, Branka
AU  - Vico, Ivana
AU  - Berenji, Janoš
AU  - Dukić, Nataša
AU  - Bulajić, Aleksandra
PY  - 2006
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1140
AB  - Tobacco can be infected with a large number of viruses and some of them are of great economic importance. Since the control of virus diseases is very complicated it makes the significance of tobacco viruses even greater. Although there are no direct chemical measures against viruses and there are no possibilities to cure diseased plants, it is still possible to control the diseases efficiently. In this respect good knowledge of biology and epidemiology of viruses is essential. There is a whole set of measures mostly preventive, in connection with the primary and secondary means of virus spread. That is why it is important to know basic virus characteristics, inoculum sources and the ways of transmission of viruses in nature described in this paper. Even though the choice of control strategy depends on the specific virus, there are some general principles shown in this article. The paper specially focuses on tobacco mosaic virus as the most important virus disease of tobacco in our environment. All sanitary measures which have to be strictly conducted in transplant and crop production have been specified. Significance of the use of resistant cultivars and efficient crop rotation were stressed. The possibilities of biological control measures as well as development of molecular strategies which result in transgenic tobacco plants resistant to tobacco mosaic virus, have also been demonstrated.
AB  - Duvan je domaćin velikog broja virusa. Neki od njih su prouzrokovači značajnih ekonomskih šteta. Zbog veoma složene kontrole virusnih oboljenja značaj virusa duvana je još veći. Iako protiv virusa nema direktnih hemijskih mera borbe i nema mogućnosti izlečenja zaraženih biljaka, ipak se može i mora sprovoditi kontrola oboljenja. Postoji čitav niz mera, pre svega preventivnih, koje su vezane za načine primarnog i sekundarnog širenja virusa. Zbog toga je neophodno poznavanje osnovnih karakteristika virusa izvora inokuluma i načina širenja virusa u prirodi. Mada izbor određene strategije kontrole zavisi od određenog virusa, postoje i opšti principi koji su izneti u ovom radu. U tom kontekstu je, u ovom radu, izneto šta je sve potrebno znati o virusu mozaika duvana kako bi se smanjila njegova štetnost u proizvodnji duvana. Pored toga, navedene su i one sanitarne mere kojih se treba strogo pridržavati i u proizvodnji rasada i pri gajenju useva duvana na otvorenom polju. Osim toga, ukazano je na značaj korišćenja otpornih sorti i primene plodoreda. Dat je pregled mogućnosti kontrole oboljenja koje pružaju biološke mere borbe, kao i razvijanje molekularnih strategija stvaranjem transgenih biljaka otpornih na virus mozaika duvana.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Zbornik radova Instituta za ratarstvo i povrtarstvo
T1  - General principles of tobacco virus diseases management with special reference to tobacco mosaic virus
T1  - Opšti principi kontrole virusnih oboljenja duvana sa posebnim osvrtom na virus mozaika duvana
EP  - 412
IS  - 1
SP  - 401
VL  - 42
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1140
ER  - 

@article{
author = "Krstić, Branka and Vico, Ivana and Berenji, Janoš and Dukić, Nataša and Bulajić, Aleksandra",
year = "2006",
abstract = "Tobacco can be infected with a large number of viruses and some of them are of great economic importance. Since the control of virus diseases is very complicated it makes the significance of tobacco viruses even greater. Although there are no direct chemical measures against viruses and there are no possibilities to cure diseased plants, it is still possible to control the diseases efficiently. In this respect good knowledge of biology and epidemiology of viruses is essential. There is a whole set of measures mostly preventive, in connection with the primary and secondary means of virus spread. That is why it is important to know basic virus characteristics, inoculum sources and the ways of transmission of viruses in nature described in this paper. Even though the choice of control strategy depends on the specific virus, there are some general principles shown in this article. The paper specially focuses on tobacco mosaic virus as the most important virus disease of tobacco in our environment. All sanitary measures which have to be strictly conducted in transplant and crop production have been specified. Significance of the use of resistant cultivars and efficient crop rotation were stressed. The possibilities of biological control measures as well as development of molecular strategies which result in transgenic tobacco plants resistant to tobacco mosaic virus, have also been demonstrated., Duvan je domaćin velikog broja virusa. Neki od njih su prouzrokovači značajnih ekonomskih šteta. Zbog veoma složene kontrole virusnih oboljenja značaj virusa duvana je još veći. Iako protiv virusa nema direktnih hemijskih mera borbe i nema mogućnosti izlečenja zaraženih biljaka, ipak se može i mora sprovoditi kontrola oboljenja. Postoji čitav niz mera, pre svega preventivnih, koje su vezane za načine primarnog i sekundarnog širenja virusa. Zbog toga je neophodno poznavanje osnovnih karakteristika virusa izvora inokuluma i načina širenja virusa u prirodi. Mada izbor određene strategije kontrole zavisi od određenog virusa, postoje i opšti principi koji su izneti u ovom radu. U tom kontekstu je, u ovom radu, izneto šta je sve potrebno znati o virusu mozaika duvana kako bi se smanjila njegova štetnost u proizvodnji duvana. Pored toga, navedene su i one sanitarne mere kojih se treba strogo pridržavati i u proizvodnji rasada i pri gajenju useva duvana na otvorenom polju. Osim toga, ukazano je na značaj korišćenja otpornih sorti i primene plodoreda. Dat je pregled mogućnosti kontrole oboljenja koje pružaju biološke mere borbe, kao i razvijanje molekularnih strategija stvaranjem transgenih biljaka otpornih na virus mozaika duvana.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Zbornik radova Instituta za ratarstvo i povrtarstvo",
title = "General principles of tobacco virus diseases management with special reference to tobacco mosaic virus, Opšti principi kontrole virusnih oboljenja duvana sa posebnim osvrtom na virus mozaika duvana",
pages = "412-401",
number = "1",
volume = "42",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1140"
}

Krstić, B., Vico, I., Berenji, J., Dukić, N.,& Bulajić, A.. (2006). General principles of tobacco virus diseases management with special reference to tobacco mosaic virus. in Zbornik radova Instituta za ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 42(1), 401-412.
https://hdl.handle.net/21.15107/rcub_agrospace_1140

Krstić B, Vico I, Berenji J, Dukić N, Bulajić A. General principles of tobacco virus diseases management with special reference to tobacco mosaic virus. in Zbornik radova Instituta za ratarstvo i povrtarstvo. 2006;42(1):401-412.
https://hdl.handle.net/21.15107/rcub_agrospace_1140 .

Krstić, Branka, Vico, Ivana, Berenji, Janoš, Dukić, Nataša, Bulajić, Aleksandra, "General principles of tobacco virus diseases management with special reference to tobacco mosaic virus" in Zbornik radova Instituta za ratarstvo i povrtarstvo, 42, no. 1 (2006):401-412,
https://hdl.handle.net/21.15107/rcub_agrospace_1140 .

TY  - JOUR
AU  - Dukić, N
AU  - Krstić, B
AU  - Vico, Ivana
AU  - Berenji, Janoš
AU  - Duduk, Bojan
PY  - 2006
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1328
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First report of Zucchini yellow mosaic virus, Watermelon mosaic virus, and Cucumber mosaic virus in bottlegourd (Lagenaria siceraria) in Serbia.
EP  - 380
IS  - 3
SP  - 380
VL  - 90
DO  - 10.1094/PD-90-0380A
ER  - 

@article{
author = "Dukić, N and Krstić, B and Vico, Ivana and Berenji, Janoš and Duduk, Bojan",
year = "2006",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First report of Zucchini yellow mosaic virus, Watermelon mosaic virus, and Cucumber mosaic virus in bottlegourd (Lagenaria siceraria) in Serbia.",
pages = "380-380",
number = "3",
volume = "90",
doi = "10.1094/PD-90-0380A"
}

Dukić, N., Krstić, B., Vico, I., Berenji, J.,& Duduk, B.. (2006). First report of Zucchini yellow mosaic virus, Watermelon mosaic virus, and Cucumber mosaic virus in bottlegourd (Lagenaria siceraria) in Serbia.. in Plant Disease
Amer Phytopathological Soc, St Paul., 90(3), 380-380.
https://doi.org/10.1094/PD-90-0380A

Dukić N, Krstić B, Vico I, Berenji J, Duduk B. First report of Zucchini yellow mosaic virus, Watermelon mosaic virus, and Cucumber mosaic virus in bottlegourd (Lagenaria siceraria) in Serbia.. in Plant Disease. 2006;90(3):380-380.
doi:10.1094/PD-90-0380A .

Dukić, N, Krstić, B, Vico, Ivana, Berenji, Janoš, Duduk, Bojan, "First report of Zucchini yellow mosaic virus, Watermelon mosaic virus, and Cucumber mosaic virus in bottlegourd (Lagenaria siceraria) in Serbia." in Plant Disease, 90, no. 3 (2006):380-380,
https://doi.org/10.1094/PD-90-0380A . .

TY  - JOUR
AU  - Dukić, Nataša
AU  - Krstić, Branka
AU  - Berenji, Janoš
PY  - 2006
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1169
AB  - Cucurbits are very important crops in many countries. The most significant cucurbits diseases are those caused by viruses. Concerning pumpkin virus diseases, three viruses were detected in Serbia after 2000: Cucumber mosaic virus (CMV), Watermelon mosaic virus 2 (WMV-2) and Zucchini yellow mosaic virus (ZYMV). Viral infections symptoms on pumpkins are very severe and variable. Disease appearance and spread depends on the causal virus. There are different control measures for viral diseases, but most of them are based on prevention.
AB  - Vrste familije Cucurbitaceae predstavljaju značajnu grupu gajenih biljaka u mnogim zemljama sveta. U proizvodnji, prvo mesto po značaju zauzimaju bolesti virusne prirode. Sprovedenim ispitivanjima od 2000. godine utvrđeno je prisustvo tri virusa na običnoj tikvi (virus mozaika krastavca - Cucumber mosaic virus, CMV; virus mozaika lubenice 2 - Watermelon mosaic virus 2, WMV-2; virus žutog mozaika cukinija - Zucchini yellow mosaic virus, ZYMV). Simptomi virusnih zaraza mogu biti veoma izraženi i raznovrsni. Pojava i širenje bolesti uslovljena je virusnim prouzrokovačem. Postoje različite mere zaštite od virusnih bolesti, koje su usmerene na preventivu i prekidanje epidemiološkog lanca bolesti.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Cucurbit viral diseases
T1  - Virusna oboljenja tikve
EP  - 235
IS  - 3
SP  - 230
VL  - 34
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1169
ER  - 

@article{
author = "Dukić, Nataša and Krstić, Branka and Berenji, Janoš",
year = "2006",
abstract = "Cucurbits are very important crops in many countries. The most significant cucurbits diseases are those caused by viruses. Concerning pumpkin virus diseases, three viruses were detected in Serbia after 2000: Cucumber mosaic virus (CMV), Watermelon mosaic virus 2 (WMV-2) and Zucchini yellow mosaic virus (ZYMV). Viral infections symptoms on pumpkins are very severe and variable. Disease appearance and spread depends on the causal virus. There are different control measures for viral diseases, but most of them are based on prevention., Vrste familije Cucurbitaceae predstavljaju značajnu grupu gajenih biljaka u mnogim zemljama sveta. U proizvodnji, prvo mesto po značaju zauzimaju bolesti virusne prirode. Sprovedenim ispitivanjima od 2000. godine utvrđeno je prisustvo tri virusa na običnoj tikvi (virus mozaika krastavca - Cucumber mosaic virus, CMV; virus mozaika lubenice 2 - Watermelon mosaic virus 2, WMV-2; virus žutog mozaika cukinija - Zucchini yellow mosaic virus, ZYMV). Simptomi virusnih zaraza mogu biti veoma izraženi i raznovrsni. Pojava i širenje bolesti uslovljena je virusnim prouzrokovačem. Postoje različite mere zaštite od virusnih bolesti, koje su usmerene na preventivu i prekidanje epidemiološkog lanca bolesti.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Cucurbit viral diseases, Virusna oboljenja tikve",
pages = "235-230",
number = "3",
volume = "34",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1169"
}

Dukić, N., Krstić, B.,& Berenji, J.. (2006). Cucurbit viral diseases. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 34(3), 230-235.
https://hdl.handle.net/21.15107/rcub_agrospace_1169

Dukić N, Krstić B, Berenji J. Cucurbit viral diseases. in Biljni lekar. 2006;34(3):230-235.
https://hdl.handle.net/21.15107/rcub_agrospace_1169 .

Dukić, Nataša, Krstić, Branka, Berenji, Janoš, "Cucurbit viral diseases" in Biljni lekar, 34, no. 3 (2006):230-235,
https://hdl.handle.net/21.15107/rcub_agrospace_1169 .

TY  - JOUR
AU  - Dukić, Nataša
AU  - Berenji, Janoš
AU  - Krstić, Branka
AU  - Vico, Ivana
AU  - Bulajić, Aleksandra
PY  - 2004
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/859
AB  - Screenings conducted in 2002 indicated the occurrence of viral symptoms on pumpkins in the locations of Bački Petrovac, Futog, Žabalj, Titel, Torda and Veliko Selo. Using two methods of viral identification, biotest and DAS−ELISA serological method, we have confirmed the presence of three viruses, CMV, WMV−2 and ZYMV, in pumpkin samples. ZYMV was the predominant virus of pumpkins in 2002. WMV−2 differed significantly in the frequency of occurrence between years. While it occurred sporadically in 2001, it was the second most frequent virus in 2002. CMV was in the second place in 2001 and in the third place in 2002. The observed variability in the occurrence and spread of viruses among the years is frequent in the case of no persistent viruses of pumpkins. It is also an indication of possible expansion of WMV−2 in Serbia.
AB  - Ispitivanjima sprovedenim tokom 2002. godine zabeležena je pojava virusnih simptoma na običnoj tikvi u lokalitetima Bački Petrovac, Futog, Žabalj Titel, Torda i Veliko Selo. Primenom dve metode identifikacije virusa biotesta i serološke metode, DAS−ELISA, u uzrocima obične tikve utvrđeno je prisustvo tri virusa CMV, WMV−2 i ZYMV. Praćenjem pojave i rasprostranjenosti virusa na običnoj tikvi tokom 2002. godine, potvrđeno je dominantno prisustvo ZYMV. Značajna razlika u učestalosti pojave, u odnosu na ispitivanja ranijih godina, zabeležena je kod WMV−2, koji se u 2001. javljao sporadično, a u 2002. godini bio drugi po zastupljenosti. CMV je tokom 2001. godine bio drugi po zastupljenosti, a tokom 2002. godine treći. Ova varijabilnost u pojavi i rasprostranjenosti virusa u različitim godinama, česta je pojava kod neperzistentnih virusa na gajenim tikvama, ali govori i o mogućoj ekspanziji WMV−2 u našoj zemlji.
PB  - Naučni institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Bilten za hmelj, sirak i lekovito bilje
T1  - Occurrence and spread of viral diseases on pumpkins (Cucurbita pepo L) in the Vojvodina province (Serbia)
T1  - Prisustvo i rasprostranjenost viroza obične tikve (Cucurbita pepo L) u Vojvodini
EP  - 79
IS  - 76-77
SP  - 71
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_859
ER  - 

@article{
author = "Dukić, Nataša and Berenji, Janoš and Krstić, Branka and Vico, Ivana and Bulajić, Aleksandra",
year = "2004",
abstract = "Screenings conducted in 2002 indicated the occurrence of viral symptoms on pumpkins in the locations of Bački Petrovac, Futog, Žabalj, Titel, Torda and Veliko Selo. Using two methods of viral identification, biotest and DAS−ELISA serological method, we have confirmed the presence of three viruses, CMV, WMV−2 and ZYMV, in pumpkin samples. ZYMV was the predominant virus of pumpkins in 2002. WMV−2 differed significantly in the frequency of occurrence between years. While it occurred sporadically in 2001, it was the second most frequent virus in 2002. CMV was in the second place in 2001 and in the third place in 2002. The observed variability in the occurrence and spread of viruses among the years is frequent in the case of no persistent viruses of pumpkins. It is also an indication of possible expansion of WMV−2 in Serbia., Ispitivanjima sprovedenim tokom 2002. godine zabeležena je pojava virusnih simptoma na običnoj tikvi u lokalitetima Bački Petrovac, Futog, Žabalj Titel, Torda i Veliko Selo. Primenom dve metode identifikacije virusa biotesta i serološke metode, DAS−ELISA, u uzrocima obične tikve utvrđeno je prisustvo tri virusa CMV, WMV−2 i ZYMV. Praćenjem pojave i rasprostranjenosti virusa na običnoj tikvi tokom 2002. godine, potvrđeno je dominantno prisustvo ZYMV. Značajna razlika u učestalosti pojave, u odnosu na ispitivanja ranijih godina, zabeležena je kod WMV−2, koji se u 2001. javljao sporadično, a u 2002. godini bio drugi po zastupljenosti. CMV je tokom 2001. godine bio drugi po zastupljenosti, a tokom 2002. godine treći. Ova varijabilnost u pojavi i rasprostranjenosti virusa u različitim godinama, česta je pojava kod neperzistentnih virusa na gajenim tikvama, ali govori i o mogućoj ekspanziji WMV−2 u našoj zemlji.",
publisher = "Naučni institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Bilten za hmelj, sirak i lekovito bilje",
title = "Occurrence and spread of viral diseases on pumpkins (Cucurbita pepo L) in the Vojvodina province (Serbia), Prisustvo i rasprostranjenost viroza obične tikve (Cucurbita pepo L) u Vojvodini",
pages = "79-71",
number = "76-77",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_859"
}

Dukić, N., Berenji, J., Krstić, B., Vico, I.,& Bulajić, A.. (2004). Occurrence and spread of viral diseases on pumpkins (Cucurbita pepo L) in the Vojvodina province (Serbia). in Bilten za hmelj, sirak i lekovito bilje
Naučni institut za ratarstvo i povrtarstvo, Novi Sad.(76-77), 71-79.
https://hdl.handle.net/21.15107/rcub_agrospace_859

Dukić N, Berenji J, Krstić B, Vico I, Bulajić A. Occurrence and spread of viral diseases on pumpkins (Cucurbita pepo L) in the Vojvodina province (Serbia). in Bilten za hmelj, sirak i lekovito bilje. 2004;(76-77):71-79.
https://hdl.handle.net/21.15107/rcub_agrospace_859 .

Dukić, Nataša, Berenji, Janoš, Krstić, Branka, Vico, Ivana, Bulajić, Aleksandra, "Occurrence and spread of viral diseases on pumpkins (Cucurbita pepo L) in the Vojvodina province (Serbia)" in Bilten za hmelj, sirak i lekovito bilje, no. 76-77 (2004):71-79,
https://hdl.handle.net/21.15107/rcub_agrospace_859 .