TY  - JOUR
AU  - Pavlov, Jovan
AU  - Delić, Nenad
AU  - Živanović, Tomislav
AU  - Ristić, Danijela
AU  - Čamdžija, Zoran
AU  - Stevanović, Milan
AU  - Tolimir, Miodrag
PY  - 2016
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4183
AB  - Six maize inbred lines for which we assumed that have a similar genetic BACKGROUND:  were selected for the study. Inbred lines were crossed according to incomplete diallel design and fifteen hybrid combinations were obtained. Hybrid combinations and their parental lines were used in our study. The objective of the study was to examine genetic polymorphism of parental lines, as well as to determine relationships between SSR genetic distance and values of high parent heterosis and specific combining abilities for grain yield. By using 19 SSR primers we classified inbred lines in two groups. First cluster consists of inbred lines L1, L2, L3, L4 and L5, while inbred line L6 formed second cluster, indicating different heterotic BACKGROUND:  in comparison to the other five inbred lines. The Spearman's correlation coefficients between SSR based genetic distance and specific combining abilities for grain yield had a value of (r=0,53*), while positive correlation were also found between genetic distance and high parent heterosis (r=0,57*).
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Relationship between genetic distance, specific combining abilities and heterosis in maize (zea mays l.)
EP  - 172
IS  - 1
SP  - 165
VL  - 48
DO  - 10.2298/GENSR1601165P
ER  - 

@article{
author = "Pavlov, Jovan and Delić, Nenad and Živanović, Tomislav and Ristić, Danijela and Čamdžija, Zoran and Stevanović, Milan and Tolimir, Miodrag",
year = "2016",
abstract = "Six maize inbred lines for which we assumed that have a similar genetic BACKGROUND:  were selected for the study. Inbred lines were crossed according to incomplete diallel design and fifteen hybrid combinations were obtained. Hybrid combinations and their parental lines were used in our study. The objective of the study was to examine genetic polymorphism of parental lines, as well as to determine relationships between SSR genetic distance and values of high parent heterosis and specific combining abilities for grain yield. By using 19 SSR primers we classified inbred lines in two groups. First cluster consists of inbred lines L1, L2, L3, L4 and L5, while inbred line L6 formed second cluster, indicating different heterotic BACKGROUND:  in comparison to the other five inbred lines. The Spearman's correlation coefficients between SSR based genetic distance and specific combining abilities for grain yield had a value of (r=0,53*), while positive correlation were also found between genetic distance and high parent heterosis (r=0,57*).",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Relationship between genetic distance, specific combining abilities and heterosis in maize (zea mays l.)",
pages = "172-165",
number = "1",
volume = "48",
doi = "10.2298/GENSR1601165P"
}

Pavlov, J., Delić, N., Živanović, T., Ristić, D., Čamdžija, Z., Stevanović, M.,& Tolimir, M.. (2016). Relationship between genetic distance, specific combining abilities and heterosis in maize (zea mays l.). in Genetika
Društvo genetičara Srbije, Beograd., 48(1), 165-172.
https://doi.org/10.2298/GENSR1601165P

Pavlov J, Delić N, Živanović T, Ristić D, Čamdžija Z, Stevanović M, Tolimir M. Relationship between genetic distance, specific combining abilities and heterosis in maize (zea mays l.). in Genetika. 2016;48(1):165-172.
doi:10.2298/GENSR1601165P .

Pavlov, Jovan, Delić, Nenad, Živanović, Tomislav, Ristić, Danijela, Čamdžija, Zoran, Stevanović, Milan, Tolimir, Miodrag, "Relationship between genetic distance, specific combining abilities and heterosis in maize (zea mays l.)" in Genetika, 48, no. 1 (2016):165-172,
https://doi.org/10.2298/GENSR1601165P . .

TY  - JOUR
AU  - Božinović, Sofija
AU  - Prodanović, Slaven
AU  - Vančetović, Jelena
AU  - Nikolić, Ana
AU  - Ristić, Danijela
AU  - Kostadinović, Marija
AU  - Ignjatović, Dragana
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3681
AB  - Plus-hybrid effect refers to a combined effect of cytoplasmic male sterility (CMS) and xenia in maize (Zea mays L.) It could be used in commercial production by growing a mixture of 80% CMS hybrid and 20% of another fertile hybrid. The aim of this research was to examine individual and combined CMS and xenia effects on two hybrids widely grown in Serbia. Sterile and fertile versions of ZP 1 and ZP 2 hybrids (three-way; Iodent x Lancaster dents) were used as females, while ZP 1, ZP 2, ZP 3, ZP 4, and ZP 5 (three-way or single cross; Iodent (BSSS) x Lancaster dents) were used as pollinators. All of them belong to medium maturity group. The trial was set up at one location in Serbia (Zemun Polje) in 2009, 2010, and 2011. Molecular analysis of the five genotypes was done using simple sequence repeat (SSR) primers. Plus-hybrid effect on grain yield ranged from -6.2% to 6.2%; on thousand kernel weight from -1.7% to 5.2%; on number of kernels per area from -1.0% to 8.0%. The poor response could be due to a use of three-way instead of single cross hybrids in S type of sterility. Modified Rogers' distance between hybrids was in the range 0.211 to 0.378 and was not relevant for the effect, which depended mostly on the sterile hybrid genotype and the fertile hybrid pollinator ability. This approach should be more suitable for female hybrids with slightly poorer performance, already being produced on a sterile base.
PB  - Inst Investigaciones Agropecuarias, Chillan
T2  - Chilean Journal of Agricultural Research
T1  - Individual and combined (Plus-hybrid) effect of cytoplasmic male sterility and xenia on maize grain yield
EP  - 167
IS  - 2
SP  - 160
VL  - 75
DO  - 10.4067/S0718-58392015000200004
ER  - 

@article{
author = "Božinović, Sofija and Prodanović, Slaven and Vančetović, Jelena and Nikolić, Ana and Ristić, Danijela and Kostadinović, Marija and Ignjatović, Dragana",
year = "2015",
abstract = "Plus-hybrid effect refers to a combined effect of cytoplasmic male sterility (CMS) and xenia in maize (Zea mays L.) It could be used in commercial production by growing a mixture of 80% CMS hybrid and 20% of another fertile hybrid. The aim of this research was to examine individual and combined CMS and xenia effects on two hybrids widely grown in Serbia. Sterile and fertile versions of ZP 1 and ZP 2 hybrids (three-way; Iodent x Lancaster dents) were used as females, while ZP 1, ZP 2, ZP 3, ZP 4, and ZP 5 (three-way or single cross; Iodent (BSSS) x Lancaster dents) were used as pollinators. All of them belong to medium maturity group. The trial was set up at one location in Serbia (Zemun Polje) in 2009, 2010, and 2011. Molecular analysis of the five genotypes was done using simple sequence repeat (SSR) primers. Plus-hybrid effect on grain yield ranged from -6.2% to 6.2%; on thousand kernel weight from -1.7% to 5.2%; on number of kernels per area from -1.0% to 8.0%. The poor response could be due to a use of three-way instead of single cross hybrids in S type of sterility. Modified Rogers' distance between hybrids was in the range 0.211 to 0.378 and was not relevant for the effect, which depended mostly on the sterile hybrid genotype and the fertile hybrid pollinator ability. This approach should be more suitable for female hybrids with slightly poorer performance, already being produced on a sterile base.",
publisher = "Inst Investigaciones Agropecuarias, Chillan",
journal = "Chilean Journal of Agricultural Research",
title = "Individual and combined (Plus-hybrid) effect of cytoplasmic male sterility and xenia on maize grain yield",
pages = "167-160",
number = "2",
volume = "75",
doi = "10.4067/S0718-58392015000200004"
}

Božinović, S., Prodanović, S., Vančetović, J., Nikolić, A., Ristić, D., Kostadinović, M.,& Ignjatović, D.. (2015). Individual and combined (Plus-hybrid) effect of cytoplasmic male sterility and xenia on maize grain yield. in Chilean Journal of Agricultural Research
Inst Investigaciones Agropecuarias, Chillan., 75(2), 160-167.
https://doi.org/10.4067/S0718-58392015000200004

Božinović S, Prodanović S, Vančetović J, Nikolić A, Ristić D, Kostadinović M, Ignjatović D. Individual and combined (Plus-hybrid) effect of cytoplasmic male sterility and xenia on maize grain yield. in Chilean Journal of Agricultural Research. 2015;75(2):160-167.
doi:10.4067/S0718-58392015000200004 .

Božinović, Sofija, Prodanović, Slaven, Vančetović, Jelena, Nikolić, Ana, Ristić, Danijela, Kostadinović, Marija, Ignjatović, Dragana, "Individual and combined (Plus-hybrid) effect of cytoplasmic male sterility and xenia on maize grain yield" in Chilean Journal of Agricultural Research, 75, no. 2 (2015):160-167,
https://doi.org/10.4067/S0718-58392015000200004 . .

TY  - JOUR
AU  - Milojević, Katarina
AU  - Stanković, Ivana
AU  - Vučurović, Ana
AU  - Ristić, Danijela
AU  - Nikolić, Dušan
AU  - Bulajić, Aleksandra
AU  - Krstić, Branka
PY  - 2013
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3242
AB  - Investigation of the presence and distribution of Cucumber mosaic virus in watermelon crops in Serbia during 2012 revealed that the virus was present in five out of six surveyed localities. CMV was detected in 24.05% of total serologically tested samples using DAS-ELI­SA method and the virus was the most prevalent in the locality of Silbaš. During this investigation, Cucumber mosaic virus was present more often in mixed infections with ZYMV and/or WMV (12.66%), while the single infection was detected in lower percentage, 11.39%. Five CMV isolates were obtained by mechanical inoculations of N. glutinosa and two of them were selected for further biological characterization. Concerning type and severity of symptoms on test plants, the investigated isolates exhibited different phenotypic features. Molecular detection was performed by amplification of a fragment of 871 bp in all tested isolates, using the primer pair CMVCPfwd/CPrev that amplify the entire coat protein gene and part of 5' and 3' UTR regions. The affiliation of Serbian CMV isolates from watermelon to subgroup IA, the group that includes most of the isolates selected for phylogenetic analysis, was determined by sequence analysis of the complete CP gene and reconstruction of phylogenetic tree.
AB  - Proučavanjem pojave i rasprostranjenosti virusa mozaika krastavca u usevu lubenice u Srbiji tokom 2012. godine, prisustvo virusa utvrđeno je u pet od šest pregledanih lokaliteta. Prisustvo virusa ustanovljeno je u 24,05% serološki testiranih uzoraka (DAS-ELISA), a najzastupljeniji je bio na lokalitetu Silbaš. Tokom ovih istraživanja virus mozaika krastavca češće je bio prisutan u mešanim infekcijama sa ZYMV i/ili WMV (12,66%), dok je prisustvo pojedinačne infekcije utvrđeno u nešto nižem procentu, 11,39%. Mehaničkim inokulacijama N. glutinosa, dobijeno je pet izolata, od kojih su dva odabrana za dalju biološku karakterizaciju. Na osnovu tipa i jačine simptoma na test-biljkama, utvrđena je fenotipska varijabilnost ispitivanih izolata. Molekularna detekcija obavljena je amplifikacijom fragmenta dužine 871 bp kod svih ispitivanih izolata korišćenjem para prajmera CMVCPfwd/CPrev, koji omogućavaju umnožavanje gena za protein omotača i deo 5' i 3' UTR regiona. Analizom sekvenci kompletnog gena za protein omotača i rekonstrukcijom filogenetskog stabla, utvrđena je pripadnost izolata CMV iz lubenice iz Srbije podgrupi IA, koja obuhvata većinu izolata odabranih za filogenetsku analizu.
PB  - Institut za zaštitu bilja i životnu sredinu, Beograd
T2  - Zaštita bilja
T1  - Biological and molecular characterization of cucumber mosaic virus infecting watermelon in Serbia
T1  - Biološka i molekularna karakterizacija virusa mozaika krastavca poreklom iz lubenice u Srbiji
EP  - 25
IS  - 1
SP  - 14
VL  - 64
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_3242
ER  - 

@article{
author = "Milojević, Katarina and Stanković, Ivana and Vučurović, Ana and Ristić, Danijela and Nikolić, Dušan and Bulajić, Aleksandra and Krstić, Branka",
year = "2013",
abstract = "Investigation of the presence and distribution of Cucumber mosaic virus in watermelon crops in Serbia during 2012 revealed that the virus was present in five out of six surveyed localities. CMV was detected in 24.05% of total serologically tested samples using DAS-ELI­SA method and the virus was the most prevalent in the locality of Silbaš. During this investigation, Cucumber mosaic virus was present more often in mixed infections with ZYMV and/or WMV (12.66%), while the single infection was detected in lower percentage, 11.39%. Five CMV isolates were obtained by mechanical inoculations of N. glutinosa and two of them were selected for further biological characterization. Concerning type and severity of symptoms on test plants, the investigated isolates exhibited different phenotypic features. Molecular detection was performed by amplification of a fragment of 871 bp in all tested isolates, using the primer pair CMVCPfwd/CPrev that amplify the entire coat protein gene and part of 5' and 3' UTR regions. The affiliation of Serbian CMV isolates from watermelon to subgroup IA, the group that includes most of the isolates selected for phylogenetic analysis, was determined by sequence analysis of the complete CP gene and reconstruction of phylogenetic tree., Proučavanjem pojave i rasprostranjenosti virusa mozaika krastavca u usevu lubenice u Srbiji tokom 2012. godine, prisustvo virusa utvrđeno je u pet od šest pregledanih lokaliteta. Prisustvo virusa ustanovljeno je u 24,05% serološki testiranih uzoraka (DAS-ELISA), a najzastupljeniji je bio na lokalitetu Silbaš. Tokom ovih istraživanja virus mozaika krastavca češće je bio prisutan u mešanim infekcijama sa ZYMV i/ili WMV (12,66%), dok je prisustvo pojedinačne infekcije utvrđeno u nešto nižem procentu, 11,39%. Mehaničkim inokulacijama N. glutinosa, dobijeno je pet izolata, od kojih su dva odabrana za dalju biološku karakterizaciju. Na osnovu tipa i jačine simptoma na test-biljkama, utvrđena je fenotipska varijabilnost ispitivanih izolata. Molekularna detekcija obavljena je amplifikacijom fragmenta dužine 871 bp kod svih ispitivanih izolata korišćenjem para prajmera CMVCPfwd/CPrev, koji omogućavaju umnožavanje gena za protein omotača i deo 5' i 3' UTR regiona. Analizom sekvenci kompletnog gena za protein omotača i rekonstrukcijom filogenetskog stabla, utvrđena je pripadnost izolata CMV iz lubenice iz Srbije podgrupi IA, koja obuhvata većinu izolata odabranih za filogenetsku analizu.",
publisher = "Institut za zaštitu bilja i životnu sredinu, Beograd",
journal = "Zaštita bilja",
title = "Biological and molecular characterization of cucumber mosaic virus infecting watermelon in Serbia, Biološka i molekularna karakterizacija virusa mozaika krastavca poreklom iz lubenice u Srbiji",
pages = "25-14",
number = "1",
volume = "64",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_3242"
}

Milojević, K., Stanković, I., Vučurović, A., Ristić, D., Nikolić, D., Bulajić, A.,& Krstić, B.. (2013). Biological and molecular characterization of cucumber mosaic virus infecting watermelon in Serbia. in Zaštita bilja
Institut za zaštitu bilja i životnu sredinu, Beograd., 64(1), 14-25.
https://hdl.handle.net/21.15107/rcub_agrospace_3242

Milojević K, Stanković I, Vučurović A, Ristić D, Nikolić D, Bulajić A, Krstić B. Biological and molecular characterization of cucumber mosaic virus infecting watermelon in Serbia. in Zaštita bilja. 2013;64(1):14-25.
https://hdl.handle.net/21.15107/rcub_agrospace_3242 .

Milojević, Katarina, Stanković, Ivana, Vučurović, Ana, Ristić, Danijela, Nikolić, Dušan, Bulajić, Aleksandra, Krstić, Branka, "Biological and molecular characterization of cucumber mosaic virus infecting watermelon in Serbia" in Zaštita bilja, 64, no. 1 (2013):14-25,
https://hdl.handle.net/21.15107/rcub_agrospace_3242 .

TY  - JOUR
AU  - Ristić, Danijela
AU  - Stanković, Ivana
AU  - Vučurović, Ana
AU  - Berenji, Janoš
AU  - Krnjajić, Slobodan
AU  - Krstić, Branka
AU  - Bulajić, Aleksandra
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2936
AB  - Sixteen samples of sorghum seed (Sorghum bicolor (L.) Moench.) 'Alba', 'Gold', 'Prima' and 'Reform' were analyzed in the localities of Bački Petrovac and Čantavir in the period 2009-2011. Tipresence of species belonging to the genera Epicoccum, Fusarium, Alternaria, Aspergillus and Penicillium was established in single and mixed infections. From the infected sorghum seed, monosporial cultures identified as Epicoccum nigrum based on morphology, proved their pathogenicity on artificially inoculated sorghum seedlings. Molecular identification was performed by PCR and amplification of the ITS region of ribosomal DNA. Gene sequences of selected isolates 291-09 (JQ619838) and 315-09 (JQ619839) exhibited 99-100% nucleotide identity with the sequences of 31 isolates of E. nigrum deposited in the GenBank. It obtained results represent the first detailed characterization of E. nigrum in Serbia. The presence of a large number of phytopathogenic fungi on sorghum seed should be further investigated in order to clarify their relationships and relative significance.
AB  - U periodu 2009-2011. godine na lokalitetima Bački Petrovac i Čantavir prikupljeno je i analizirano 16 uzoraka zaraženog semena gajenog sirka (Sorghum bicolor (L.) Moench.) sorti Alba, Gold, Prima i Reform na prisustvo fitopatogenih gljiva. U pojedinačnim i mešanim zarazama ustanovljeno je prisustvo vrsta iz rodova Epicoccum, Fusarium, Alternaria, Aspergillus i Penicillium. Iz zaraženog semena izolovane su monosporne kulture i na osnovu morfoloških osobina identifikovane su kao Epicoccum nigrum. Patogenost izolata ove gljive potvrđena je pojavom simptoma na veštački inokulisanim sejancima sirka. Molekularna identifikacija obavljena je primenom PCR i amplifikacije ITS regiona ribozomalne DNK. Sekvence gena odabranih izolata 291-09 (JQ619838) i 315-09 (JQ619839) pokazale su 99-100% nukleotidne identičnosti sa sekvencama 31 izolata E. nigrum deponovanihu GenBank bazi podataka. Dobijeni rezultati predstavljaju prvu detaljnu karakterizaciju E. nigrum u Srbiji. Prisustvo većeg broja vrsta gljiva na semenu sirka zahteva dalja ispitivanja njihovih međusobnih odnosa i značaja.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Epicoccum nigrum the new pathogen of sorghum seed in Serbia
T1  - Epicoccum nigrum novi patogen semena sirka u Srbiji
EP  - 166
IS  - 2
SP  - 160
VL  - 49
DO  - 10.5937/ratpov49-1793
ER  - 

@article{
author = "Ristić, Danijela and Stanković, Ivana and Vučurović, Ana and Berenji, Janoš and Krnjajić, Slobodan and Krstić, Branka and Bulajić, Aleksandra",
year = "2012",
abstract = "Sixteen samples of sorghum seed (Sorghum bicolor (L.) Moench.) 'Alba', 'Gold', 'Prima' and 'Reform' were analyzed in the localities of Bački Petrovac and Čantavir in the period 2009-2011. Tipresence of species belonging to the genera Epicoccum, Fusarium, Alternaria, Aspergillus and Penicillium was established in single and mixed infections. From the infected sorghum seed, monosporial cultures identified as Epicoccum nigrum based on morphology, proved their pathogenicity on artificially inoculated sorghum seedlings. Molecular identification was performed by PCR and amplification of the ITS region of ribosomal DNA. Gene sequences of selected isolates 291-09 (JQ619838) and 315-09 (JQ619839) exhibited 99-100% nucleotide identity with the sequences of 31 isolates of E. nigrum deposited in the GenBank. It obtained results represent the first detailed characterization of E. nigrum in Serbia. The presence of a large number of phytopathogenic fungi on sorghum seed should be further investigated in order to clarify their relationships and relative significance., U periodu 2009-2011. godine na lokalitetima Bački Petrovac i Čantavir prikupljeno je i analizirano 16 uzoraka zaraženog semena gajenog sirka (Sorghum bicolor (L.) Moench.) sorti Alba, Gold, Prima i Reform na prisustvo fitopatogenih gljiva. U pojedinačnim i mešanim zarazama ustanovljeno je prisustvo vrsta iz rodova Epicoccum, Fusarium, Alternaria, Aspergillus i Penicillium. Iz zaraženog semena izolovane su monosporne kulture i na osnovu morfoloških osobina identifikovane su kao Epicoccum nigrum. Patogenost izolata ove gljive potvrđena je pojavom simptoma na veštački inokulisanim sejancima sirka. Molekularna identifikacija obavljena je primenom PCR i amplifikacije ITS regiona ribozomalne DNK. Sekvence gena odabranih izolata 291-09 (JQ619838) i 315-09 (JQ619839) pokazale su 99-100% nukleotidne identičnosti sa sekvencama 31 izolata E. nigrum deponovanihu GenBank bazi podataka. Dobijeni rezultati predstavljaju prvu detaljnu karakterizaciju E. nigrum u Srbiji. Prisustvo većeg broja vrsta gljiva na semenu sirka zahteva dalja ispitivanja njihovih međusobnih odnosa i značaja.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Epicoccum nigrum the new pathogen of sorghum seed in Serbia, Epicoccum nigrum novi patogen semena sirka u Srbiji",
pages = "166-160",
number = "2",
volume = "49",
doi = "10.5937/ratpov49-1793"
}

Ristić, D., Stanković, I., Vučurović, A., Berenji, J., Krnjajić, S., Krstić, B.,& Bulajić, A.. (2012). Epicoccum nigrum the new pathogen of sorghum seed in Serbia. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 49(2), 160-166.
https://doi.org/10.5937/ratpov49-1793

Ristić D, Stanković I, Vučurović A, Berenji J, Krnjajić S, Krstić B, Bulajić A. Epicoccum nigrum the new pathogen of sorghum seed in Serbia. in Ratarstvo i povrtarstvo. 2012;49(2):160-166.
doi:10.5937/ratpov49-1793 .

Ristić, Danijela, Stanković, Ivana, Vučurović, Ana, Berenji, Janoš, Krnjajić, Slobodan, Krstić, Branka, Bulajić, Aleksandra, "Epicoccum nigrum the new pathogen of sorghum seed in Serbia" in Ratarstvo i povrtarstvo, 49, no. 2 (2012):160-166,
https://doi.org/10.5937/ratpov49-1793 . .

TY  - JOUR
AU  - Vučurović, Ana
AU  - Bulajić, Aleksandra
AU  - Milojević, Katarina
AU  - Stanković, Ivana
AU  - Ristić, Danijela
AU  - Berenji, Janoš
AU  - Krstić, Branka
PY  - 2012
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2935
AB  - The presence of Zucchini yellow mosaic virus (ZYMV) in two out of seven watermelon production localities in Serbia during 2011 was investigated by analyzing leaves sampled from symptomatic and asymptomatic watermelon plants and utilizing DAS-ELISA test. In the locality of Gornji Tavankut, ZYMV was detected in 23.08% of tested plants in single infections, and in the locality of Silbas it was detected in 35.29% of tested plants in mixed infections with Cucumber mosaic virus and Alfalfa mosaic virus. ZYMV was successfully mechanically transmitted from naturally infected watermelon plants to Cucurbita pepo 'Ezra F1'. Molecular detection was performed by RT-PCR and amplification of part of the gene for nuclear inclusions, gene of coat protein and part of 3' non-coding region, which confirmed the identification of the ZYMV isolates. Phylogenetic analysis revealed grouping of the isolate originating from watermelon with other isolates from Serbia and Central Europe within A-I subgroup. Analysis of amino acid sequences of the N terminal end of the CP gene revealed that isolate 550-11 belongs to the Central European branch.
AB  - Prisustvo virusa žutog mozaika cukinija (Zucchini yellow mosaic virus, ZYMV) na dva od sedam lokaliteta gajenja lubenice u Srbiji tokom 2011. godine utvrđeno je analizom uzoraka lišća simptomatičnih i asimptomatičnih biljaka lubenice primenom DAS-ELISA metode. Na lokalitetu Gornji Tavankut, ZYMV je dokazan u 23,08% testiranih biljaka u pojedinačnim infekcijama, a na lokalitetu Silbaš u 35,29% testiranih biljaka u mešanim infekcijama sa virusom mozaika krastavca i virusom mozaika lubenice. ZYMV je uspešno mehanički prenesen sa prirodno zaraženih biljaka lubenice na Cucurbita pepo 'Ezra F1'. Molekularna detekcija obavljena je RT-PCR metodom umnožavanjem dela gena za nuklearne inkluzije, gena za protein omotača i dela 3' neprepisujućeg regiona, čime je potvrđena identifikacija izolata. Filogenetske analize pokazale su grupisanje izolata poreklom iz lubenice sa drugim izolatima iz Srbije i Centralne Evrope u okviru A-I podgrupe. Analize aminokiselinske sekvence N-terminalnog kraja CP gena, takođe su pokazale pripadnost izolata 550-11 centralnoevropskoj grani.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Presence and characterization of Zucchini yellow mosaic virus in watermelon in Serbia
T1  - Prisustvo i karakterizacija virusa žutog mozaika cukinija u usevu lubenice u Srbiji
EP  - 159
IS  - 2
SP  - 151
VL  - 49
DO  - 10.5937/ratpov49-1773
ER  - 

@article{
author = "Vučurović, Ana and Bulajić, Aleksandra and Milojević, Katarina and Stanković, Ivana and Ristić, Danijela and Berenji, Janoš and Krstić, Branka",
year = "2012",
abstract = "The presence of Zucchini yellow mosaic virus (ZYMV) in two out of seven watermelon production localities in Serbia during 2011 was investigated by analyzing leaves sampled from symptomatic and asymptomatic watermelon plants and utilizing DAS-ELISA test. In the locality of Gornji Tavankut, ZYMV was detected in 23.08% of tested plants in single infections, and in the locality of Silbas it was detected in 35.29% of tested plants in mixed infections with Cucumber mosaic virus and Alfalfa mosaic virus. ZYMV was successfully mechanically transmitted from naturally infected watermelon plants to Cucurbita pepo 'Ezra F1'. Molecular detection was performed by RT-PCR and amplification of part of the gene for nuclear inclusions, gene of coat protein and part of 3' non-coding region, which confirmed the identification of the ZYMV isolates. Phylogenetic analysis revealed grouping of the isolate originating from watermelon with other isolates from Serbia and Central Europe within A-I subgroup. Analysis of amino acid sequences of the N terminal end of the CP gene revealed that isolate 550-11 belongs to the Central European branch., Prisustvo virusa žutog mozaika cukinija (Zucchini yellow mosaic virus, ZYMV) na dva od sedam lokaliteta gajenja lubenice u Srbiji tokom 2011. godine utvrđeno je analizom uzoraka lišća simptomatičnih i asimptomatičnih biljaka lubenice primenom DAS-ELISA metode. Na lokalitetu Gornji Tavankut, ZYMV je dokazan u 23,08% testiranih biljaka u pojedinačnim infekcijama, a na lokalitetu Silbaš u 35,29% testiranih biljaka u mešanim infekcijama sa virusom mozaika krastavca i virusom mozaika lubenice. ZYMV je uspešno mehanički prenesen sa prirodno zaraženih biljaka lubenice na Cucurbita pepo 'Ezra F1'. Molekularna detekcija obavljena je RT-PCR metodom umnožavanjem dela gena za nuklearne inkluzije, gena za protein omotača i dela 3' neprepisujućeg regiona, čime je potvrđena identifikacija izolata. Filogenetske analize pokazale su grupisanje izolata poreklom iz lubenice sa drugim izolatima iz Srbije i Centralne Evrope u okviru A-I podgrupe. Analize aminokiselinske sekvence N-terminalnog kraja CP gena, takođe su pokazale pripadnost izolata 550-11 centralnoevropskoj grani.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Presence and characterization of Zucchini yellow mosaic virus in watermelon in Serbia, Prisustvo i karakterizacija virusa žutog mozaika cukinija u usevu lubenice u Srbiji",
pages = "159-151",
number = "2",
volume = "49",
doi = "10.5937/ratpov49-1773"
}

Vučurović, A., Bulajić, A., Milojević, K., Stanković, I., Ristić, D., Berenji, J.,& Krstić, B.. (2012). Presence and characterization of Zucchini yellow mosaic virus in watermelon in Serbia. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 49(2), 151-159.
https://doi.org/10.5937/ratpov49-1773

Vučurović A, Bulajić A, Milojević K, Stanković I, Ristić D, Berenji J, Krstić B. Presence and characterization of Zucchini yellow mosaic virus in watermelon in Serbia. in Ratarstvo i povrtarstvo. 2012;49(2):151-159.
doi:10.5937/ratpov49-1773 .

Vučurović, Ana, Bulajić, Aleksandra, Milojević, Katarina, Stanković, Ivana, Ristić, Danijela, Berenji, Janoš, Krstić, Branka, "Presence and characterization of Zucchini yellow mosaic virus in watermelon in Serbia" in Ratarstvo i povrtarstvo, 49, no. 2 (2012):151-159,
https://doi.org/10.5937/ratpov49-1773 . .

TY  - JOUR
AU  - Ristić, Danijela
AU  - Vučurović, Ana
AU  - Stanković, Ivana
AU  - Nikolić, Dušan
AU  - Berenji, Janoš
AU  - Krstić, Branka
AU  - Bulajić, Aleksandra
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2608
AB  - A total of 39 samples of sorghum (Sorghum bicolor) with symptoms of stem and root rot were collected and analyzed during 2009-2011 in Bački Petrovac and Čantavir, Serbia. Monosporic cultures were isolated from stem tissue, their pathogenicity was confirmed by the development of symptoms on artificially inoculated sorghum plants, and they were identified on the basis of macroscopic and microscopic morphological features as Fusarium graminearum. Molecular identification was performed utilizing polimerase chain reaction (PCR) with primer pair ef1/ ef2 and by amplification of protein coding TEF 1-alpha gen. Sequence of TEF gene from the selected isolate 535- 10 (JF747146) showed 98-99% nucleotide identity with sequences of 63 Gibberella zeae isolates deposited in NCBI GenBank. Amplification of the barcoding region of F. graminearum genome of sorghum isolate, contributes to the fast and accurate identification and characterization of Fusarium species in Serbia.
AB  - U periodu 2009-2011. na lokalitetima u Bačkom Petrovcu i Čantaviru prikupljeno je i analizirano 39 uzoraka biljaka gajenog sirka (Sorghum bicolor) sa simptomima truleži prizemnog dela stabla. Iz biljnog tkiva izolovane su monosporne kulture, čija je patogenost potvrđena pojavom simptoma na veštački inokulisanim biljkama sirka, a na osnovu morfoloških makroskopskih i mikroskopskih osobina identifikovan je Fusarium graminearum. Molekularna identifikacija obavljena je primenom lančane reakcije polimeraze (PCR, Polymerase Chain Reaction) uz korišćenje prajmera ef1/ef2 i amplifikaciju kodirajućeg proteinskog gena TEF 1-alfa. Sekvenca TEF gena odabranog izolata 535-10 (JF747146) je pokazala 98% do 99% nukleotidne identičnosti sa sekvencama 63 izolata Gibberella zeae deponovanih u NCBI bazi podataka. Amplifikacijom barkoding dela genoma F. graminearum izolata iz sirka dat je doprinos bržoj i preciznijoj identifikaciji i karakterizaciji vrsta roda Fusarium u Srbiji.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo
T1  - Molecular identification of Fusarium graminearum, sorghum pathogen in Serbia
T1  - Molekularna identifikacija izolata Fusarium graminearum, patogena sirka u Srbiji
EP  - 352
IS  - 2
SP  - 347
VL  - 48
DO  - 10.5937/ratpov1102347R
ER  - 

@article{
author = "Ristić, Danijela and Vučurović, Ana and Stanković, Ivana and Nikolić, Dušan and Berenji, Janoš and Krstić, Branka and Bulajić, Aleksandra",
year = "2011",
abstract = "A total of 39 samples of sorghum (Sorghum bicolor) with symptoms of stem and root rot were collected and analyzed during 2009-2011 in Bački Petrovac and Čantavir, Serbia. Monosporic cultures were isolated from stem tissue, their pathogenicity was confirmed by the development of symptoms on artificially inoculated sorghum plants, and they were identified on the basis of macroscopic and microscopic morphological features as Fusarium graminearum. Molecular identification was performed utilizing polimerase chain reaction (PCR) with primer pair ef1/ ef2 and by amplification of protein coding TEF 1-alpha gen. Sequence of TEF gene from the selected isolate 535- 10 (JF747146) showed 98-99% nucleotide identity with sequences of 63 Gibberella zeae isolates deposited in NCBI GenBank. Amplification of the barcoding region of F. graminearum genome of sorghum isolate, contributes to the fast and accurate identification and characterization of Fusarium species in Serbia., U periodu 2009-2011. na lokalitetima u Bačkom Petrovcu i Čantaviru prikupljeno je i analizirano 39 uzoraka biljaka gajenog sirka (Sorghum bicolor) sa simptomima truleži prizemnog dela stabla. Iz biljnog tkiva izolovane su monosporne kulture, čija je patogenost potvrđena pojavom simptoma na veštački inokulisanim biljkama sirka, a na osnovu morfoloških makroskopskih i mikroskopskih osobina identifikovan je Fusarium graminearum. Molekularna identifikacija obavljena je primenom lančane reakcije polimeraze (PCR, Polymerase Chain Reaction) uz korišćenje prajmera ef1/ef2 i amplifikaciju kodirajućeg proteinskog gena TEF 1-alfa. Sekvenca TEF gena odabranog izolata 535-10 (JF747146) je pokazala 98% do 99% nukleotidne identičnosti sa sekvencama 63 izolata Gibberella zeae deponovanih u NCBI bazi podataka. Amplifikacijom barkoding dela genoma F. graminearum izolata iz sirka dat je doprinos bržoj i preciznijoj identifikaciji i karakterizaciji vrsta roda Fusarium u Srbiji.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo",
title = "Molecular identification of Fusarium graminearum, sorghum pathogen in Serbia, Molekularna identifikacija izolata Fusarium graminearum, patogena sirka u Srbiji",
pages = "352-347",
number = "2",
volume = "48",
doi = "10.5937/ratpov1102347R"
}

Ristić, D., Vučurović, A., Stanković, I., Nikolić, D., Berenji, J., Krstić, B.,& Bulajić, A.. (2011). Molecular identification of Fusarium graminearum, sorghum pathogen in Serbia. in Ratarstvo i povrtarstvo
Institut za ratarstvo i povrtarstvo, Novi Sad., 48(2), 347-352.
https://doi.org/10.5937/ratpov1102347R

Ristić D, Vučurović A, Stanković I, Nikolić D, Berenji J, Krstić B, Bulajić A. Molecular identification of Fusarium graminearum, sorghum pathogen in Serbia. in Ratarstvo i povrtarstvo. 2011;48(2):347-352.
doi:10.5937/ratpov1102347R .

Ristić, Danijela, Vučurović, Ana, Stanković, Ivana, Nikolić, Dušan, Berenji, Janoš, Krstić, Branka, Bulajić, Aleksandra, "Molecular identification of Fusarium graminearum, sorghum pathogen in Serbia" in Ratarstvo i povrtarstvo, 48, no. 2 (2011):347-352,
https://doi.org/10.5937/ratpov1102347R . .

TY  - JOUR
AU  - Vučurović, Ana
AU  - Bulajić, Aleksandra
AU  - Stanković, Ivana
AU  - Ristić, Danijela
AU  - Berenji, Janoš
AU  - Krstić, Branka
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2539
AB  - Cucumber mosaic virus (CMV) is considered one of the most economically important plant viruses and has a worldwide distribution and a very wide host range including plants from family Cucurbitaceae. In Serbia, on cucurbits CMV was detected in single and mixed infections with Zucchini yellow mosaic virus (ZYMV) and Watermelon mosaic virus (WMV). Viruses, including CMV, are constantly present in cucurbit crops, but their frequency changes by year and locality. Surveys and sample collections were conducted in cucurbit crops in the period from 2008 to 2009 at 15 localities in Vojvodina province, and sample testing was carried out using the DAS-ELISA method and commercially available antisera for six economically most important cucurbit viruses. In 2008, a total of 51 samples were collected from 13 cucurbit crops of oilseed pumpkin Olinka variety, squash, and bottle gourd and CMV was detected in a total of 55% of tested samples with symptoms of viral infection. The most common infectious type was mixed infection with ZYMV and WMV (35.3%), and then mixed infection with ZYMV (17.7%) and WMV (2%). A total of 599 symptomatic samples of oilseed pumpkin Olinka variety, zucchini squash varieties Beogradska and Tosca, squash, and winter squash were collected in 15 cucurbits crops in 2009. CMV was present in 4.4% of total collected samples, in single infections in 1.3%, and in mixed with WMV or ZYMV in 1.3%, and 1.8%. Five CMV isolates were obtained by mechanical inoculations of N. glutinosa and one of them was selected for further biological characterization. Test plants which were described to be hosts of CMV expressed symptoms characteristic for those caused by CMV after inoculations by isolate 115-08. CMV specific primers Au1u/Au2d were used to amplify an 850 bp fragment using RT-PCR method. Amplified fragment encodes the entire viral coat protein (CP) gene and partial 5' and 3' UTRs of two selected CMV isolates. Amplified fragments were sequenced and deposited in the NCBI, where they were assigned accession numbers, HM065510 (115-08) and HM065509 (151-08). The sequences of CMV isolates from Serbia shared the highest nucleotide and amino acid identity with isolates from subgroup IA, from 99.5 to 97.4% and 99.1 to 97.4%, and the lowest identities were with the subgroup II isolates from 66.9 to 64, 5%, from 75.8 to 74.1%.
AB  - Virus mozaika krastavca (Cucumber mosaic virus, CMV) je veoma široko rasprostranjen i jedan je od ekonomski najznačajnijih virusa za poljoprivrednu proizvodnju, sa veoma širokim krugom domaćina u koji spada i veliki broj gajenih biljaka, među kojima i predstavnici familije Cucurbitaceae. Epidemiologija ovog virusa je veoma složena, zbog čega je i njegova kontrola otežana. Na tikvama u Srbiji CMV je detektovan u pojedinačnim i mešanim infekcijama sa virusom žutog mozaika cukinija (Zucchini yellow mosaic virus, ZYMV) i virusom mozaika lubenice (Watermelon mosaic virus, WMV). Virusi, pa tako i CMV, su stalno prisutni u usevima tikava, ali se njihova učestalost menja po godinama i lokalitetima. Pregledi terena i sakupljanje uzoraka tikava obavljeni su u periodu 2008-2009. godine na 15 lokaliteta gajenja tikava u Vojvodini, a testiranje sakupljenih uzoraka izvršeno je primenom DAS-ELISA metode i komercijalno dostupnih antiseruma za šest ekonomski najznačajnih virusa tikava u svetu. Tokom 2008. godine u 13 useva gajenih tikava sakupljen je ukupno 51 uzorak uljane tikve sorte Olinka, bundeve i vrga, a CMV je detektovan u ukupno 55% testiranih uzoraka sa simptomima virusnih zaraza. CMV je najčešće bio detektovan u mešanoj infekciji sa ZYMV i WMV (35,3%), a zatim u mešanoj infekciji sa ZYMV (17,7%) i sa WMV (2%). Ukupno 599 simptomatičnih uzoraka uljane tikve sorte Olinka, tikvica sorti Beogradska i Toska, bundeve i muskatne tikve sakupljeno je u 15 useva tokom 2009. godine. U sakupljenim uzorcima CMV je bio prisutan u ukupno 4,4% testiranih uzoraka. U pojedinačnim infekcijama u 1,3%, a u mešanim sa WMV ili ZYMV u 1,3%, odnosno 1,8% testiranih uzoraka. Mehaničkim inokulacijama N. glutinosa dobijeno je pet izolata CMV od kojih je jedan izabran za dalju biološku karakterizaciju. Odabrani izolat (115-08) izazvao je na test biljkama koje su opisane kao domaćini CMV pojavu karakterističnih simptoma. Specifičnim prajmerima CMV Au1u/Au2d uz upotrebu RT-PCR metode umnožen je deo genoma od oko 850 bp koji kodira ceo protein omotača virusa i delove 5' i 3' neprepisujućih regiona dva odabrana izolata CMV. Amplifikovani fragmenti su sekvencionirani i prijavljeni u NCBI, gde su im dodeljeni pristupni brojevi, HM065510 (115-08) i HM065509 (151-08). Sekvence CMV izolata iz Srbije dele najveću nukleotidnu i aminokiselinsku sličnost sa izolatima iz podgrupe IA, od 99,5 do 97,4%, odnosno 99,1 do 97,4%, a najmanju sa izolatima II podgrupe od 66,9 do 64,5%, odnosno od 75,8 do 74,1%.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Characterization of cucumber mosaic virus originating from cucurbits in Serbia
T1  - Karakterizacija virusa mozaika krastavca poreklom iz tikava u Srbiji
EP  - 336
IS  - 4
SP  - 325
VL  - 26
DO  - 10.2298/PIF1104325V
ER  - 

@article{
author = "Vučurović, Ana and Bulajić, Aleksandra and Stanković, Ivana and Ristić, Danijela and Berenji, Janoš and Krstić, Branka",
year = "2011",
abstract = "Cucumber mosaic virus (CMV) is considered one of the most economically important plant viruses and has a worldwide distribution and a very wide host range including plants from family Cucurbitaceae. In Serbia, on cucurbits CMV was detected in single and mixed infections with Zucchini yellow mosaic virus (ZYMV) and Watermelon mosaic virus (WMV). Viruses, including CMV, are constantly present in cucurbit crops, but their frequency changes by year and locality. Surveys and sample collections were conducted in cucurbit crops in the period from 2008 to 2009 at 15 localities in Vojvodina province, and sample testing was carried out using the DAS-ELISA method and commercially available antisera for six economically most important cucurbit viruses. In 2008, a total of 51 samples were collected from 13 cucurbit crops of oilseed pumpkin Olinka variety, squash, and bottle gourd and CMV was detected in a total of 55% of tested samples with symptoms of viral infection. The most common infectious type was mixed infection with ZYMV and WMV (35.3%), and then mixed infection with ZYMV (17.7%) and WMV (2%). A total of 599 symptomatic samples of oilseed pumpkin Olinka variety, zucchini squash varieties Beogradska and Tosca, squash, and winter squash were collected in 15 cucurbits crops in 2009. CMV was present in 4.4% of total collected samples, in single infections in 1.3%, and in mixed with WMV or ZYMV in 1.3%, and 1.8%. Five CMV isolates were obtained by mechanical inoculations of N. glutinosa and one of them was selected for further biological characterization. Test plants which were described to be hosts of CMV expressed symptoms characteristic for those caused by CMV after inoculations by isolate 115-08. CMV specific primers Au1u/Au2d were used to amplify an 850 bp fragment using RT-PCR method. Amplified fragment encodes the entire viral coat protein (CP) gene and partial 5' and 3' UTRs of two selected CMV isolates. Amplified fragments were sequenced and deposited in the NCBI, where they were assigned accession numbers, HM065510 (115-08) and HM065509 (151-08). The sequences of CMV isolates from Serbia shared the highest nucleotide and amino acid identity with isolates from subgroup IA, from 99.5 to 97.4% and 99.1 to 97.4%, and the lowest identities were with the subgroup II isolates from 66.9 to 64, 5%, from 75.8 to 74.1%., Virus mozaika krastavca (Cucumber mosaic virus, CMV) je veoma široko rasprostranjen i jedan je od ekonomski najznačajnijih virusa za poljoprivrednu proizvodnju, sa veoma širokim krugom domaćina u koji spada i veliki broj gajenih biljaka, među kojima i predstavnici familije Cucurbitaceae. Epidemiologija ovog virusa je veoma složena, zbog čega je i njegova kontrola otežana. Na tikvama u Srbiji CMV je detektovan u pojedinačnim i mešanim infekcijama sa virusom žutog mozaika cukinija (Zucchini yellow mosaic virus, ZYMV) i virusom mozaika lubenice (Watermelon mosaic virus, WMV). Virusi, pa tako i CMV, su stalno prisutni u usevima tikava, ali se njihova učestalost menja po godinama i lokalitetima. Pregledi terena i sakupljanje uzoraka tikava obavljeni su u periodu 2008-2009. godine na 15 lokaliteta gajenja tikava u Vojvodini, a testiranje sakupljenih uzoraka izvršeno je primenom DAS-ELISA metode i komercijalno dostupnih antiseruma za šest ekonomski najznačajnih virusa tikava u svetu. Tokom 2008. godine u 13 useva gajenih tikava sakupljen je ukupno 51 uzorak uljane tikve sorte Olinka, bundeve i vrga, a CMV je detektovan u ukupno 55% testiranih uzoraka sa simptomima virusnih zaraza. CMV je najčešće bio detektovan u mešanoj infekciji sa ZYMV i WMV (35,3%), a zatim u mešanoj infekciji sa ZYMV (17,7%) i sa WMV (2%). Ukupno 599 simptomatičnih uzoraka uljane tikve sorte Olinka, tikvica sorti Beogradska i Toska, bundeve i muskatne tikve sakupljeno je u 15 useva tokom 2009. godine. U sakupljenim uzorcima CMV je bio prisutan u ukupno 4,4% testiranih uzoraka. U pojedinačnim infekcijama u 1,3%, a u mešanim sa WMV ili ZYMV u 1,3%, odnosno 1,8% testiranih uzoraka. Mehaničkim inokulacijama N. glutinosa dobijeno je pet izolata CMV od kojih je jedan izabran za dalju biološku karakterizaciju. Odabrani izolat (115-08) izazvao je na test biljkama koje su opisane kao domaćini CMV pojavu karakterističnih simptoma. Specifičnim prajmerima CMV Au1u/Au2d uz upotrebu RT-PCR metode umnožen je deo genoma od oko 850 bp koji kodira ceo protein omotača virusa i delove 5' i 3' neprepisujućih regiona dva odabrana izolata CMV. Amplifikovani fragmenti su sekvencionirani i prijavljeni u NCBI, gde su im dodeljeni pristupni brojevi, HM065510 (115-08) i HM065509 (151-08). Sekvence CMV izolata iz Srbije dele najveću nukleotidnu i aminokiselinsku sličnost sa izolatima iz podgrupe IA, od 99,5 do 97,4%, odnosno 99,1 do 97,4%, a najmanju sa izolatima II podgrupe od 66,9 do 64,5%, odnosno od 75,8 do 74,1%.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Characterization of cucumber mosaic virus originating from cucurbits in Serbia, Karakterizacija virusa mozaika krastavca poreklom iz tikava u Srbiji",
pages = "336-325",
number = "4",
volume = "26",
doi = "10.2298/PIF1104325V"
}

Vučurović, A., Bulajić, A., Stanković, I., Ristić, D., Berenji, J.,& Krstić, B.. (2011). Characterization of cucumber mosaic virus originating from cucurbits in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 26(4), 325-336.
https://doi.org/10.2298/PIF1104325V

Vučurović A, Bulajić A, Stanković I, Ristić D, Berenji J, Krstić B. Characterization of cucumber mosaic virus originating from cucurbits in Serbia. in Pesticidi i fitomedicina. 2011;26(4):325-336.
doi:10.2298/PIF1104325V .

Vučurović, Ana, Bulajić, Aleksandra, Stanković, Ivana, Ristić, Danijela, Berenji, Janoš, Krstić, Branka, "Characterization of cucumber mosaic virus originating from cucurbits in Serbia" in Pesticidi i fitomedicina, 26, no. 4 (2011):325-336,
https://doi.org/10.2298/PIF1104325V . .

TY  - JOUR
AU  - Stanković, Ivana
AU  - Vučurović, Ana
AU  - Bulajić, Aleksandra
AU  - Ristić, Danijela
AU  - Berenji, Janoš
AU  - Krstić, Branka
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2532
AB  - Three-year investigation of the presence and distribution of tobacco viruses in Serbia revealed that Alfalfa mosaic virus (AMV) appeared every year with different frequency in tobacco crops. During 2008, the presence of AMV was detected in most of the tested samples (58.82%) and it was the second most common compared to all other viruses which presence was confirmed in Serbia. In 2006 and 2007, AMV was detected in a significantly lower percentage (2.80% and 13.64%, respectively). This study showed that Alfalfa mosaic virus was more commonly found in multiple infections with two, three or even four detected viruses. Single infections were detected only in 2006, in one tobacco field in the locality of Futog. During this investigation, a rapid and simple protocol was optimized and developed for molecular detection of AMV in tobacco leaves, using primers CPAMV1/CPAMV2 and commercially available kits for total RNA extraction as well as for RT-PCR (reverse transcription - polymerase chain reaction). Using RT-PCR and these primers that flank the AMV coat protein gene, a DNA fragment of 751 bp was amplified, sequenced, and compared with the sequences available in GenBank database. The sequence of isolate 196-08 (GenBank Acc. No. FJ527749) proved to be identical at the nucleotide level of 99 to 93% with those from other parts of the world. Phylogenetic analysis of 27 isolates based on 528 bp sequences of the coat protein gene did not show correlation of the isolates with their geographic origin or plant host and showed that these isolates fall into four molecular groups of strains. Serbian AMV isolate from tobacco belongs to group IV, the group that includes most of the isolates selected for phylogenetic analysis.
AB  - Trogodišnjim proučavanjima pojave i rasprostranjenosti virusa u usevu duvana u Srbiji utvrđeno je da se virus mozaika lucerke (Alfalfa mosaic virus, AMV) javlja svake godine sa različitom učestalošću. Prisustvo AMV ustanovljeno je u najvećem broju testiranih uzoraka (58,82%) tokom 2008. godine kada je bio drugi po zastupljenosti u odnosu na sve ostale viruse čije je prisustvo utvrđeno u Srbiji. AMV je detektovan u znatno nižem procentu 2006. (2,80%) i 2007. godine (13,64%). Tokom ovih istraživanja virus mozaika lucerke češće je bio prisutan u mešanim infekcijama sa dva, tri ili čak četiri detektovana virusa. Prisustvo pojedinačne zaraze utvrđeno je samo 2006. godine u jednom usevu duvana na lokalitetu Futog. U toku ovih istraživanja razvijen je i optimiziran brz i precizan protokol za molekularnu detekciju AMV u lišću duvana primenom specifičnih prajmera CPAMV1/CPAMV2 i komercijalnih kitova za ekstrakciju ukupnih RNK i RT-PCR (reverzna transkripcija praćena lančanom reakcijom polimeraze). Korišćenjem ovih prajmera, koji omogućavaju umnožavanje gena za protein omotača, umnožen je fragment dužine 751 bp, sekvencioniran i upoređen sa sekvencama dostupnim u GenBank bazi podataka. Dobijena sekvenca izolata 196-08 (GenBank Acc. No. FJ527749) pokazala je 99-93% nukleotidne identičnosti sa izolatima AMV iz drugih delova sveta. Filogenetske analize 27 izolata, na osnovu 528 bp sekvence gena za protein omotača, nisu ukazale na korelaciju sa geografskim poreklom izolata ili biljkom domaćinom, a pokazale su grupisanje izolata u četiri molekularne grupe sojeva. Izolat AMV iz duvana iz Srbije pripada grupi IV, koja obuhvata većinu izolata odabranih za filogenetsku analizu.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Presence and molecular characterization of alfalfa mosaic virus on tobacco in Serbia
T1  - Prisustvo i molekularna karakterizacija virusa mozaika lucerke u usevu duvana u Srbiji
EP  - 243
IS  - 3
SP  - 229
VL  - 26
DO  - 10.2298/PIF1103229S
ER  - 

@article{
author = "Stanković, Ivana and Vučurović, Ana and Bulajić, Aleksandra and Ristić, Danijela and Berenji, Janoš and Krstić, Branka",
year = "2011",
abstract = "Three-year investigation of the presence and distribution of tobacco viruses in Serbia revealed that Alfalfa mosaic virus (AMV) appeared every year with different frequency in tobacco crops. During 2008, the presence of AMV was detected in most of the tested samples (58.82%) and it was the second most common compared to all other viruses which presence was confirmed in Serbia. In 2006 and 2007, AMV was detected in a significantly lower percentage (2.80% and 13.64%, respectively). This study showed that Alfalfa mosaic virus was more commonly found in multiple infections with two, three or even four detected viruses. Single infections were detected only in 2006, in one tobacco field in the locality of Futog. During this investigation, a rapid and simple protocol was optimized and developed for molecular detection of AMV in tobacco leaves, using primers CPAMV1/CPAMV2 and commercially available kits for total RNA extraction as well as for RT-PCR (reverse transcription - polymerase chain reaction). Using RT-PCR and these primers that flank the AMV coat protein gene, a DNA fragment of 751 bp was amplified, sequenced, and compared with the sequences available in GenBank database. The sequence of isolate 196-08 (GenBank Acc. No. FJ527749) proved to be identical at the nucleotide level of 99 to 93% with those from other parts of the world. Phylogenetic analysis of 27 isolates based on 528 bp sequences of the coat protein gene did not show correlation of the isolates with their geographic origin or plant host and showed that these isolates fall into four molecular groups of strains. Serbian AMV isolate from tobacco belongs to group IV, the group that includes most of the isolates selected for phylogenetic analysis., Trogodišnjim proučavanjima pojave i rasprostranjenosti virusa u usevu duvana u Srbiji utvrđeno je da se virus mozaika lucerke (Alfalfa mosaic virus, AMV) javlja svake godine sa različitom učestalošću. Prisustvo AMV ustanovljeno je u najvećem broju testiranih uzoraka (58,82%) tokom 2008. godine kada je bio drugi po zastupljenosti u odnosu na sve ostale viruse čije je prisustvo utvrđeno u Srbiji. AMV je detektovan u znatno nižem procentu 2006. (2,80%) i 2007. godine (13,64%). Tokom ovih istraživanja virus mozaika lucerke češće je bio prisutan u mešanim infekcijama sa dva, tri ili čak četiri detektovana virusa. Prisustvo pojedinačne zaraze utvrđeno je samo 2006. godine u jednom usevu duvana na lokalitetu Futog. U toku ovih istraživanja razvijen je i optimiziran brz i precizan protokol za molekularnu detekciju AMV u lišću duvana primenom specifičnih prajmera CPAMV1/CPAMV2 i komercijalnih kitova za ekstrakciju ukupnih RNK i RT-PCR (reverzna transkripcija praćena lančanom reakcijom polimeraze). Korišćenjem ovih prajmera, koji omogućavaju umnožavanje gena za protein omotača, umnožen je fragment dužine 751 bp, sekvencioniran i upoređen sa sekvencama dostupnim u GenBank bazi podataka. Dobijena sekvenca izolata 196-08 (GenBank Acc. No. FJ527749) pokazala je 99-93% nukleotidne identičnosti sa izolatima AMV iz drugih delova sveta. Filogenetske analize 27 izolata, na osnovu 528 bp sekvence gena za protein omotača, nisu ukazale na korelaciju sa geografskim poreklom izolata ili biljkom domaćinom, a pokazale su grupisanje izolata u četiri molekularne grupe sojeva. Izolat AMV iz duvana iz Srbije pripada grupi IV, koja obuhvata većinu izolata odabranih za filogenetsku analizu.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Presence and molecular characterization of alfalfa mosaic virus on tobacco in Serbia, Prisustvo i molekularna karakterizacija virusa mozaika lucerke u usevu duvana u Srbiji",
pages = "243-229",
number = "3",
volume = "26",
doi = "10.2298/PIF1103229S"
}

Stanković, I., Vučurović, A., Bulajić, A., Ristić, D., Berenji, J.,& Krstić, B.. (2011). Presence and molecular characterization of alfalfa mosaic virus on tobacco in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 26(3), 229-243.
https://doi.org/10.2298/PIF1103229S

Stanković I, Vučurović A, Bulajić A, Ristić D, Berenji J, Krstić B. Presence and molecular characterization of alfalfa mosaic virus on tobacco in Serbia. in Pesticidi i fitomedicina. 2011;26(3):229-243.
doi:10.2298/PIF1103229S .

Stanković, Ivana, Vučurović, Ana, Bulajić, Aleksandra, Ristić, Danijela, Berenji, Janoš, Krstić, Branka, "Presence and molecular characterization of alfalfa mosaic virus on tobacco in Serbia" in Pesticidi i fitomedicina, 26, no. 3 (2011):229-243,
https://doi.org/10.2298/PIF1103229S . .

TY  - JOUR
AU  - Ristić, Danijela
AU  - Vučurović, Ana
AU  - Stanković, Ivana
AU  - Jović, Jelena
AU  - Milojević, Katarina
AU  - Bulajić, Aleksandra
AU  - Krstić, Branka
PY  - 2011
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2518
AB  - During 2010, Impatiens walleriana plants with symptoms of downy mildew were collected in a greenhouse in the vicinity of Mionica, Kolubara District. Disease incidence was extremely high, approaching 100%, and wilting and collapse of affected plants was very rapid, resulting in losses of more than 90%. White downy growth produced on the lower leaf surface consisted of hyaline, thin-walled sporangiophores with monopodial branching and numerous, ovoid and hyaline sporangia. Apical branchlets of sporangiophores were at right angles to the main axis, with no apical thickening. Pathogenicity tests included inoculation of young I. walleriana plants by spraying with a sporangial suspension, and downy mildew symptoms were observed after 13 to 15 days. The absence of well-defined spots on the infected impatiens leaves and straight sporangiophores indicated that the pathogen was P. obducens, which was further supported by molecular identification, the 5'-end of the nuclear DNA coding for the large ribosomal subunit (LSU rDNA) was amplified by PCR, using primers NL1 and NL4. A representative isolate, 28-10, was sequenced and phylogenetic analysis showed its grouping with other P. obducens isolates of different origin. Considering that impatiens downy mildew in Serbia is proved to be caused by P. obducens it is necessary to employ adequate phytosanitary measures to prevent further spread of the pathogen.
AB  - Tokom 2010. godine biljke Impatiens walleriana sa simptomima plamenjače sakupljene su u stakleniku u okolini Mionice, Kolubarski okrug. Učestalost oboljenja bila je izuzetno visoka, blizu 100%, a sušenje i propadanje zaraženih biljaka veoma brzo, što je dovelo do šteta većih od 90%. Bela prevlaka patogena koja se razvijala na naličju listova sastojala se od hijalinskih monopodijalno razgranatih sporangiofora sa tankim zidovima i brojnih, ovoidnih i hijalinskih sporangija. Vrhovi grana sporangiofora granali su se pod pravim uglom u odnosu na glavnu osu, bez vršnog zadebljanja. Testovi patogenosti uključili su inokulacije mladih biljaka I. walleriana prskanjem suspenzijom sporangija, a simptomi plamenjače razvili su se nakon 13-15 dana. Odsustvo definisanih pega na zaraženim listovima impatiensa i prisustvo pravih sporangiofora ukazalo je da je prouzrokovač P. obducens, što je dalje potvrđeno molekularnom identifikacijom. Primenom lančane reakcije polimeraze (polymerase chain reaction, PCR), pomoću prajmera NL1 i NL4, umnožen je kodirajući 5'-kraj DNA velike ribozomalne podjedinice (LSU rDNA). Amplifikovani produkt odabranog izolata 28-10 je sekvencioniran, a filogenetske analize pokazale su njegovo grupisanje sa ostalim izolatima P. obducens različitog porekla. Uzimajući u obzir da je ovim istraživanjima dokazano da je prouzrokovač plamenjače na Impatiens walleriana u Srbiji P. obducens, neophodno je preduzimanje odgovarajućih fitosanitarnih mera u cilju sprečavanja daljeg širenja patogena u našoj zemlji.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Plasmopara obducens: A new threat to the production of Impatiens Walleriana in Serbia
T1  - Plasmopara obducens - nova pretnja proizvodnji Impatiens Walleriana u Srbiji
EP  - 53
IS  - 1
SP  - 43
VL  - 26
DO  - 10.2298/PIF1101043R
ER  - 

@article{
author = "Ristić, Danijela and Vučurović, Ana and Stanković, Ivana and Jović, Jelena and Milojević, Katarina and Bulajić, Aleksandra and Krstić, Branka",
year = "2011",
abstract = "During 2010, Impatiens walleriana plants with symptoms of downy mildew were collected in a greenhouse in the vicinity of Mionica, Kolubara District. Disease incidence was extremely high, approaching 100%, and wilting and collapse of affected plants was very rapid, resulting in losses of more than 90%. White downy growth produced on the lower leaf surface consisted of hyaline, thin-walled sporangiophores with monopodial branching and numerous, ovoid and hyaline sporangia. Apical branchlets of sporangiophores were at right angles to the main axis, with no apical thickening. Pathogenicity tests included inoculation of young I. walleriana plants by spraying with a sporangial suspension, and downy mildew symptoms were observed after 13 to 15 days. The absence of well-defined spots on the infected impatiens leaves and straight sporangiophores indicated that the pathogen was P. obducens, which was further supported by molecular identification, the 5'-end of the nuclear DNA coding for the large ribosomal subunit (LSU rDNA) was amplified by PCR, using primers NL1 and NL4. A representative isolate, 28-10, was sequenced and phylogenetic analysis showed its grouping with other P. obducens isolates of different origin. Considering that impatiens downy mildew in Serbia is proved to be caused by P. obducens it is necessary to employ adequate phytosanitary measures to prevent further spread of the pathogen., Tokom 2010. godine biljke Impatiens walleriana sa simptomima plamenjače sakupljene su u stakleniku u okolini Mionice, Kolubarski okrug. Učestalost oboljenja bila je izuzetno visoka, blizu 100%, a sušenje i propadanje zaraženih biljaka veoma brzo, što je dovelo do šteta većih od 90%. Bela prevlaka patogena koja se razvijala na naličju listova sastojala se od hijalinskih monopodijalno razgranatih sporangiofora sa tankim zidovima i brojnih, ovoidnih i hijalinskih sporangija. Vrhovi grana sporangiofora granali su se pod pravim uglom u odnosu na glavnu osu, bez vršnog zadebljanja. Testovi patogenosti uključili su inokulacije mladih biljaka I. walleriana prskanjem suspenzijom sporangija, a simptomi plamenjače razvili su se nakon 13-15 dana. Odsustvo definisanih pega na zaraženim listovima impatiensa i prisustvo pravih sporangiofora ukazalo je da je prouzrokovač P. obducens, što je dalje potvrđeno molekularnom identifikacijom. Primenom lančane reakcije polimeraze (polymerase chain reaction, PCR), pomoću prajmera NL1 i NL4, umnožen je kodirajući 5'-kraj DNA velike ribozomalne podjedinice (LSU rDNA). Amplifikovani produkt odabranog izolata 28-10 je sekvencioniran, a filogenetske analize pokazale su njegovo grupisanje sa ostalim izolatima P. obducens različitog porekla. Uzimajući u obzir da je ovim istraživanjima dokazano da je prouzrokovač plamenjače na Impatiens walleriana u Srbiji P. obducens, neophodno je preduzimanje odgovarajućih fitosanitarnih mera u cilju sprečavanja daljeg širenja patogena u našoj zemlji.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Plasmopara obducens: A new threat to the production of Impatiens Walleriana in Serbia, Plasmopara obducens - nova pretnja proizvodnji Impatiens Walleriana u Srbiji",
pages = "53-43",
number = "1",
volume = "26",
doi = "10.2298/PIF1101043R"
}

Ristić, D., Vučurović, A., Stanković, I., Jović, J., Milojević, K., Bulajić, A.,& Krstić, B.. (2011). Plasmopara obducens: A new threat to the production of Impatiens Walleriana in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 26(1), 43-53.
https://doi.org/10.2298/PIF1101043R

Ristić D, Vučurović A, Stanković I, Jović J, Milojević K, Bulajić A, Krstić B. Plasmopara obducens: A new threat to the production of Impatiens Walleriana in Serbia. in Pesticidi i fitomedicina. 2011;26(1):43-53.
doi:10.2298/PIF1101043R .

Ristić, Danijela, Vučurović, Ana, Stanković, Ivana, Jović, Jelena, Milojević, Katarina, Bulajić, Aleksandra, Krstić, Branka, "Plasmopara obducens: A new threat to the production of Impatiens Walleriana in Serbia" in Pesticidi i fitomedicina, 26, no. 1 (2011):43-53,
https://doi.org/10.2298/PIF1101043R . .

TY  - JOUR
AU  - Bulajić, Aleksandra
AU  - Vučurović, Ana
AU  - Stanković, Ivana
AU  - Ristić, Danijela
AU  - Berenji, Janoš
AU  - Krstić, Branka
PY  - 2010
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2252
AB  - As there is a growing frequency of viral plant diseases in epidemic proportions, the possibilities for successful control are constantly being explored. Despite the fact that integral and simultaneous employment of numerous control measures may contribute to the decreasing amount of yield losses, especially concerning non-persistently aphid-transmitted viruses, these measures are often not efficient enough. Research into the basis of resistance to viral infection and principles of its inheritance, introduction of sources of resistance in susceptible genotypes, by conventional or genetic manipulations, are very intensive for cucurbit crops, especially pumpkins. Pumpkin crops are being endangered by a great number of different viruses, among which the Zucchini yellow mosaic virus, (ZYMV), Watermelon mosaic virus (WMV) and Cucumber mosaic virus (CMV) are present every year in Serbia, frequently causing epidemics. The majority of pumpkin cultivars are not resistant or tolerant to viral infections, but sources of resistance have been identified in various related species. So far, the identified sources of resistance to the ZYMV are found in Cucurbita moschata and Citrullus lanatus var. lanatus genotypes and consist of one or several major dominant genes of resistance. It is a similar case with WMV, although the sources of dominant major genes are identified in C. lanatus and C. colocynthis. The sources of resistance to CMV in the form of one dominant gene have been identified in the genotype C. moschata, although the introduction of this gene by conventional means proved to be very difficult. Besides the aforementioned, substantial efforts are being made in developing genotypes with multiple resistance against several viruses and even other pathogens, as well as genotypes with resistance to the most significant plant aphid species, through mechanisms of antixenosis or antibiosis. The other way of obtaining resistant genotypes includes genetic manipulation. Genetically modified resistant pumpkins have been among the first successfully developed crops. Genotypes with pathogen derived resistance can already be found in commercially grown pumpkins in some parts of the world, and they have been developed by introducing the coat protein gene of one, two or all three viruses which are the most frequent, ZYMV, WMV and CMV. Yet, this approach to the control of pumpkin viral diseases is related to possible negative consequences, mostly through the already detected gene transfer to wild plants and development of resistant transgenic weeds of unpredictable impact on the environment. Improved host plant genetic resistance to viral infections or biological vectors, developed by conventional or genetic engineering methods, represents the most dynamic and prominent field of research. It is economically and ecologically the most justified approach to the control of pumpkin and other plant diseases caused by viruses non-persistently transmitted by aphids.
AB  - Kako je pojava brojnih viroza gajenih biljaka u epidemijskim razmerama sve češća, mogućnosti za uspešnu kontrolu stalno se ispituju. Integralna i istovremena primena brojnih pojedinačnih mera kontrole, naročito u slučaju virusa koji se prenose biljnim vašima na neperzistentan način, mogu da doprinesu smanjenju šteta, ali često nisu dovoljno efikasne. Proučavanja osnova otpornosti i načina nasleđivanja, unošenje izvora otpornosti u osetljive genotipove, konvencionalnim ili genetičkim manipulacijama, vrlo su intenzivna kod vrežastih kultura, posebno tikve. Tikve ugrožava veliki broj raznorodnih virusa od kojih se u Srbiji svake godine javljaju virus žutog mozaika cukinija (Zucchini yellow mosaic virus, ZYMV), virus mozaika lubenice (Watermelon mosaic virus, WMV) i virus mozaika krastavca (Cucumber mosaic virus, CMV), a pojedinih godina izazivaju epidemije. Većina sorti gajenih tikava ne poseduje otpornost ili tolerantnost na virusne zaraze, ali izvori otpornosti identifikovani su u različitim srodnim vrstama. Do sada identifikovani izvori otpornosti na ZYMV nalaze se u genotipovima Cucurbita moschata i Citrullus lanatus var. lanatus i obuhvataju jedan ili nekoliko major gena rezistentnosti koji se nasleđuju dominantno. Slična situacija je i sa WMV, mada su izvori dominantnih major gena identifikovani u C. lanatus i C. colocynthis. Izvor otpornosti na CMV u vidu jednog dominantnog gena identifikovan je u genotipu C. moschata, mada je unošenje ovog gena konvencionalnim putem bilo veoma teško. Pored toga, veliki napori ulažu se u dobijenje genotipova sa istovremenom otpornošću na više virusa čak i drugih patogena, kao i genotipova koji ispoljavaju otpornost na najznačajnije vrste biljnih vaši, kroz mehanizme antiksenoze ili antibioze. Drugi pravac dobijanja otpornih genotipova podrazumeva genetičke manipulacije. Genetski modifikovane otporne tikve su među prvima, od svih gajenih biljaka, uspešno razvijene. Genotipovi sa PD (pathogen derived) otpornošću već se nalaze u komercijalnoj proizvodnji tikava u nekim delovima sveta i dobijeni su unošenjem gena za CP (coat protein) jednog, dva ili sva tri u svetu najraširenija virusa, ZYMV, WMW i CMV. Ipak, ovaj prilaz kontroli virusnih oboljenja tikava vezan je za uočene moguće negativne posledice, pre svega kroz već registrovan transfer gena u biljke spontane flore i nastanak otpornih transgenih korova nepredvidljivog ponašanja i značaja u prirodi. Poboljšana genetička otpornost biljke domaćina bilo na infekciju virusima, bilo na biološke vektore, dobijena konvencionalnim ili metodama genetičkog inženjeringa predstavlja najdinamičnije i najperspektivnije polje istraživanja kao ekonomski i ekološki najopravdaniji pristup kontroli oboljenja tikava i drugih biljaka koje izazivaju virusi koji se neperzistetno prenose biljnim vašima.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Novel approaches to implementation of pumpkin resistance in control of viral diseases
T1  - Novi pravci primene otpornosti tikava u kontroli virusnih oboljenja
EP  - 211
IS  - 3
SP  - 201
VL  - 25
DO  - 10.2298/PIF1003201B
ER  - 

@article{
author = "Bulajić, Aleksandra and Vučurović, Ana and Stanković, Ivana and Ristić, Danijela and Berenji, Janoš and Krstić, Branka",
year = "2010",
abstract = "As there is a growing frequency of viral plant diseases in epidemic proportions, the possibilities for successful control are constantly being explored. Despite the fact that integral and simultaneous employment of numerous control measures may contribute to the decreasing amount of yield losses, especially concerning non-persistently aphid-transmitted viruses, these measures are often not efficient enough. Research into the basis of resistance to viral infection and principles of its inheritance, introduction of sources of resistance in susceptible genotypes, by conventional or genetic manipulations, are very intensive for cucurbit crops, especially pumpkins. Pumpkin crops are being endangered by a great number of different viruses, among which the Zucchini yellow mosaic virus, (ZYMV), Watermelon mosaic virus (WMV) and Cucumber mosaic virus (CMV) are present every year in Serbia, frequently causing epidemics. The majority of pumpkin cultivars are not resistant or tolerant to viral infections, but sources of resistance have been identified in various related species. So far, the identified sources of resistance to the ZYMV are found in Cucurbita moschata and Citrullus lanatus var. lanatus genotypes and consist of one or several major dominant genes of resistance. It is a similar case with WMV, although the sources of dominant major genes are identified in C. lanatus and C. colocynthis. The sources of resistance to CMV in the form of one dominant gene have been identified in the genotype C. moschata, although the introduction of this gene by conventional means proved to be very difficult. Besides the aforementioned, substantial efforts are being made in developing genotypes with multiple resistance against several viruses and even other pathogens, as well as genotypes with resistance to the most significant plant aphid species, through mechanisms of antixenosis or antibiosis. The other way of obtaining resistant genotypes includes genetic manipulation. Genetically modified resistant pumpkins have been among the first successfully developed crops. Genotypes with pathogen derived resistance can already be found in commercially grown pumpkins in some parts of the world, and they have been developed by introducing the coat protein gene of one, two or all three viruses which are the most frequent, ZYMV, WMV and CMV. Yet, this approach to the control of pumpkin viral diseases is related to possible negative consequences, mostly through the already detected gene transfer to wild plants and development of resistant transgenic weeds of unpredictable impact on the environment. Improved host plant genetic resistance to viral infections or biological vectors, developed by conventional or genetic engineering methods, represents the most dynamic and prominent field of research. It is economically and ecologically the most justified approach to the control of pumpkin and other plant diseases caused by viruses non-persistently transmitted by aphids., Kako je pojava brojnih viroza gajenih biljaka u epidemijskim razmerama sve češća, mogućnosti za uspešnu kontrolu stalno se ispituju. Integralna i istovremena primena brojnih pojedinačnih mera kontrole, naročito u slučaju virusa koji se prenose biljnim vašima na neperzistentan način, mogu da doprinesu smanjenju šteta, ali često nisu dovoljno efikasne. Proučavanja osnova otpornosti i načina nasleđivanja, unošenje izvora otpornosti u osetljive genotipove, konvencionalnim ili genetičkim manipulacijama, vrlo su intenzivna kod vrežastih kultura, posebno tikve. Tikve ugrožava veliki broj raznorodnih virusa od kojih se u Srbiji svake godine javljaju virus žutog mozaika cukinija (Zucchini yellow mosaic virus, ZYMV), virus mozaika lubenice (Watermelon mosaic virus, WMV) i virus mozaika krastavca (Cucumber mosaic virus, CMV), a pojedinih godina izazivaju epidemije. Većina sorti gajenih tikava ne poseduje otpornost ili tolerantnost na virusne zaraze, ali izvori otpornosti identifikovani su u različitim srodnim vrstama. Do sada identifikovani izvori otpornosti na ZYMV nalaze se u genotipovima Cucurbita moschata i Citrullus lanatus var. lanatus i obuhvataju jedan ili nekoliko major gena rezistentnosti koji se nasleđuju dominantno. Slična situacija je i sa WMV, mada su izvori dominantnih major gena identifikovani u C. lanatus i C. colocynthis. Izvor otpornosti na CMV u vidu jednog dominantnog gena identifikovan je u genotipu C. moschata, mada je unošenje ovog gena konvencionalnim putem bilo veoma teško. Pored toga, veliki napori ulažu se u dobijenje genotipova sa istovremenom otpornošću na više virusa čak i drugih patogena, kao i genotipova koji ispoljavaju otpornost na najznačajnije vrste biljnih vaši, kroz mehanizme antiksenoze ili antibioze. Drugi pravac dobijanja otpornih genotipova podrazumeva genetičke manipulacije. Genetski modifikovane otporne tikve su među prvima, od svih gajenih biljaka, uspešno razvijene. Genotipovi sa PD (pathogen derived) otpornošću već se nalaze u komercijalnoj proizvodnji tikava u nekim delovima sveta i dobijeni su unošenjem gena za CP (coat protein) jednog, dva ili sva tri u svetu najraširenija virusa, ZYMV, WMW i CMV. Ipak, ovaj prilaz kontroli virusnih oboljenja tikava vezan je za uočene moguće negativne posledice, pre svega kroz već registrovan transfer gena u biljke spontane flore i nastanak otpornih transgenih korova nepredvidljivog ponašanja i značaja u prirodi. Poboljšana genetička otpornost biljke domaćina bilo na infekciju virusima, bilo na biološke vektore, dobijena konvencionalnim ili metodama genetičkog inženjeringa predstavlja najdinamičnije i najperspektivnije polje istraživanja kao ekonomski i ekološki najopravdaniji pristup kontroli oboljenja tikava i drugih biljaka koje izazivaju virusi koji se neperzistetno prenose biljnim vašima.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Novel approaches to implementation of pumpkin resistance in control of viral diseases, Novi pravci primene otpornosti tikava u kontroli virusnih oboljenja",
pages = "211-201",
number = "3",
volume = "25",
doi = "10.2298/PIF1003201B"
}

Bulajić, A., Vučurović, A., Stanković, I., Ristić, D., Berenji, J.,& Krstić, B.. (2010). Novel approaches to implementation of pumpkin resistance in control of viral diseases. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 25(3), 201-211.
https://doi.org/10.2298/PIF1003201B

Bulajić A, Vučurović A, Stanković I, Ristić D, Berenji J, Krstić B. Novel approaches to implementation of pumpkin resistance in control of viral diseases. in Pesticidi i fitomedicina. 2010;25(3):201-211.
doi:10.2298/PIF1003201B .

Bulajić, Aleksandra, Vučurović, Ana, Stanković, Ivana, Ristić, Danijela, Berenji, Janoš, Krstić, Branka, "Novel approaches to implementation of pumpkin resistance in control of viral diseases" in Pesticidi i fitomedicina, 25, no. 3 (2010):201-211,
https://doi.org/10.2298/PIF1003201B . .

TY  - JOUR
AU  - Vučurović, Ana
AU  - Bulajić, Aleksandra
AU  - Stanković, Ivana
AU  - Ristić, Danijela
AU  - Berenji, Janoš
AU  - Krstić, Branka
PY  - 2010
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/2251
AB  - Watermelon mosaic virus (WMV) is widespread in cucurbit crops, most commonly occuring in temperate and Mediterranean regions. In Serbia WMV has been detected in single and mixed infections with Zucchini yellow mosaic virus and Cucumber mosaic virus in field-grown pumpkin and squash crops. Among pumpkin-affecting viruses WMV is the most frequent one, both by the number of localities and its incidence at each location. During the growing season of 2009, samples from 583 plants of Cucurbita pepo cvs. Olinka, Belgrade zucchini and Tosca (Zucchini group), as well as from C. maxima and C. moschata showing symptoms of virus infection were collected from 12 commercial fields at eight localities and analyzed by DAS-ELISA using polyclonal antisera specific to six most important cucurbit viruses. Interestingly, WMV was detected at fewer sites and had lower incidence rate than in two previous years. In single infections, WMV was found in 11% of tested plants in three fields; in mixed infections with ZYMV, it was recorded in 9.9% of plants in five fields and with CMV in only 0.2% in one field. The partial coat protein gene and 3' non-translated region from two representative isolates of WMV originating from different localities and host plant species were amplified by RT-PCR, sequenced, and compared with the sequences available in GenBank database. The PCR-amplified fragment of predicted size of approximately 1017 bp was obtained. The sequences of isolates 137-08 (Acc. No. GQ259958) and 159-08 (GU144020) proved to be 94-99% identical at the nucleotide level with those from other parts of the world. The sequences of these two isolates differed from each other only at two nucleotide positions, without any amino acid substitution. Phylogenetic analysis of 57 isolates based on 750 bp sequences of the coat protein gene showed no correlation between isolates and their geographic origin, and it also indicated that these isolates fell into three molecular groups of strains. Serbian WMV isolates belong to group I, the most widespread group of isolates of this virus in the world.
AB  - Virus mozaika lubenice (Watermelon mosaic virus, WMV) široko je rasprostranjen u usevima vrežastih kultura, mada se najčešće javlja na Mediteranu i regionima sa umerenom klimom. U Srbiji, WMV je detektovan u pojedinačnim i mešanim infekcijama sa virusom žutog mozaika cukinija (Zucchini yellow mosaic virus, ZYMV) i virusom mozaika krastavca (Cucumber mosaic virus, CMV) u usevima tikava i bundeva gajenim na otvorenom polju. Od virusa tikava, WMV je često prevalentan virus, kako po broju lokaliteta u kojima je dokazan, tako i po učestalosti u samom usevu. Iz ukupno 12 komercijalnih useva na osam lokaliteta, 2009. godine sakupljeno je 583 biljaka Cucurbita pepo cvs. Olinka, Beogradska tikvica i Tosca (cukini forma), kao i C. maxima i C. moschata, sa simptomima virusnih zaraza, koje su analizirane na prisustvo šest najznačajnijih virusa tikava primenom DAS-ELISA metode. WMV je detektovan na manjem broju lokaliteta i u manjoj učestalosti u poređenju sa prethodne dve vegetacije. U pojedinačnim infekcijama, WMV je detektovan u 11% testiranih biljaka u tri useva, a u mešanim sa ZYMV u 9,9% testiranih biljaka u pet useva i sa CMV u 0,2% testiranih biljaka u jednom usevu. Deo gena za protein omotača virusa i deo 3' neprepisujućeg regiona dva odabrana izolata WMV poreklom iz različitih lokaliteta i biljaka domaćina umnožen je primenom RT-PCR metode, sekvencioniran i upoređen sa sekvencama dostupnim u GenBank bazi podataka. Dobijen je PCR produkt očekivane veličine od 1017 bp. Sekvence izolata 137-08 (Acc. No. GQ259958) i 159-08 (GU144020) pokazale su 94-99% nukleotidne identičnosti sa izolatima WMV iz drugih delova sveta. Sekvence ova dva izolata razlikovala su se jedna od druge samo u dva nukleotidna mesta što nije imalo za posledicu aminokiselinske supstitucije. Filogenetske analize 57 izolata, na osnovu 750 bp sekvence gena za protein omotača, nisu ukazale na korelaciju sa geografskim poreklom izolata, a pokazala su grupisanje izolata u tri molekularne grupe sojeva. Izolati WMV iz Srbije pripadaju grupi I, grupi koja obuhvata najrasprostranjenije izolate ovog virusa u svetu.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Frequency and molecular characterization of watermelon mosaic virus from Serbia
T1  - Učestalost pojave i molekularna karakterizacija virusa mozaika lubenice poreklom iz Srbije
EP  - 230
IS  - 3
SP  - 213
VL  - 25
DO  - 10.2298/PIF1003213V
ER  - 

@article{
author = "Vučurović, Ana and Bulajić, Aleksandra and Stanković, Ivana and Ristić, Danijela and Berenji, Janoš and Krstić, Branka",
year = "2010",
abstract = "Watermelon mosaic virus (WMV) is widespread in cucurbit crops, most commonly occuring in temperate and Mediterranean regions. In Serbia WMV has been detected in single and mixed infections with Zucchini yellow mosaic virus and Cucumber mosaic virus in field-grown pumpkin and squash crops. Among pumpkin-affecting viruses WMV is the most frequent one, both by the number of localities and its incidence at each location. During the growing season of 2009, samples from 583 plants of Cucurbita pepo cvs. Olinka, Belgrade zucchini and Tosca (Zucchini group), as well as from C. maxima and C. moschata showing symptoms of virus infection were collected from 12 commercial fields at eight localities and analyzed by DAS-ELISA using polyclonal antisera specific to six most important cucurbit viruses. Interestingly, WMV was detected at fewer sites and had lower incidence rate than in two previous years. In single infections, WMV was found in 11% of tested plants in three fields; in mixed infections with ZYMV, it was recorded in 9.9% of plants in five fields and with CMV in only 0.2% in one field. The partial coat protein gene and 3' non-translated region from two representative isolates of WMV originating from different localities and host plant species were amplified by RT-PCR, sequenced, and compared with the sequences available in GenBank database. The PCR-amplified fragment of predicted size of approximately 1017 bp was obtained. The sequences of isolates 137-08 (Acc. No. GQ259958) and 159-08 (GU144020) proved to be 94-99% identical at the nucleotide level with those from other parts of the world. The sequences of these two isolates differed from each other only at two nucleotide positions, without any amino acid substitution. Phylogenetic analysis of 57 isolates based on 750 bp sequences of the coat protein gene showed no correlation between isolates and their geographic origin, and it also indicated that these isolates fell into three molecular groups of strains. Serbian WMV isolates belong to group I, the most widespread group of isolates of this virus in the world., Virus mozaika lubenice (Watermelon mosaic virus, WMV) široko je rasprostranjen u usevima vrežastih kultura, mada se najčešće javlja na Mediteranu i regionima sa umerenom klimom. U Srbiji, WMV je detektovan u pojedinačnim i mešanim infekcijama sa virusom žutog mozaika cukinija (Zucchini yellow mosaic virus, ZYMV) i virusom mozaika krastavca (Cucumber mosaic virus, CMV) u usevima tikava i bundeva gajenim na otvorenom polju. Od virusa tikava, WMV je često prevalentan virus, kako po broju lokaliteta u kojima je dokazan, tako i po učestalosti u samom usevu. Iz ukupno 12 komercijalnih useva na osam lokaliteta, 2009. godine sakupljeno je 583 biljaka Cucurbita pepo cvs. Olinka, Beogradska tikvica i Tosca (cukini forma), kao i C. maxima i C. moschata, sa simptomima virusnih zaraza, koje su analizirane na prisustvo šest najznačajnijih virusa tikava primenom DAS-ELISA metode. WMV je detektovan na manjem broju lokaliteta i u manjoj učestalosti u poređenju sa prethodne dve vegetacije. U pojedinačnim infekcijama, WMV je detektovan u 11% testiranih biljaka u tri useva, a u mešanim sa ZYMV u 9,9% testiranih biljaka u pet useva i sa CMV u 0,2% testiranih biljaka u jednom usevu. Deo gena za protein omotača virusa i deo 3' neprepisujućeg regiona dva odabrana izolata WMV poreklom iz različitih lokaliteta i biljaka domaćina umnožen je primenom RT-PCR metode, sekvencioniran i upoređen sa sekvencama dostupnim u GenBank bazi podataka. Dobijen je PCR produkt očekivane veličine od 1017 bp. Sekvence izolata 137-08 (Acc. No. GQ259958) i 159-08 (GU144020) pokazale su 94-99% nukleotidne identičnosti sa izolatima WMV iz drugih delova sveta. Sekvence ova dva izolata razlikovala su se jedna od druge samo u dva nukleotidna mesta što nije imalo za posledicu aminokiselinske supstitucije. Filogenetske analize 57 izolata, na osnovu 750 bp sekvence gena za protein omotača, nisu ukazale na korelaciju sa geografskim poreklom izolata, a pokazala su grupisanje izolata u tri molekularne grupe sojeva. Izolati WMV iz Srbije pripadaju grupi I, grupi koja obuhvata najrasprostranjenije izolate ovog virusa u svetu.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Frequency and molecular characterization of watermelon mosaic virus from Serbia, Učestalost pojave i molekularna karakterizacija virusa mozaika lubenice poreklom iz Srbije",
pages = "230-213",
number = "3",
volume = "25",
doi = "10.2298/PIF1003213V"
}

Vučurović, A., Bulajić, A., Stanković, I., Ristić, D., Berenji, J.,& Krstić, B.. (2010). Frequency and molecular characterization of watermelon mosaic virus from Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 25(3), 213-230.
https://doi.org/10.2298/PIF1003213V

Vučurović A, Bulajić A, Stanković I, Ristić D, Berenji J, Krstić B. Frequency and molecular characterization of watermelon mosaic virus from Serbia. in Pesticidi i fitomedicina. 2010;25(3):213-230.
doi:10.2298/PIF1003213V .

Vučurović, Ana, Bulajić, Aleksandra, Stanković, Ivana, Ristić, Danijela, Berenji, Janoš, Krstić, Branka, "Frequency and molecular characterization of watermelon mosaic virus from Serbia" in Pesticidi i fitomedicina, 25, no. 3 (2010):213-230,
https://doi.org/10.2298/PIF1003213V . .

TY  - JOUR
AU  - Vučurović, Ana
AU  - Bulajić, Aleksandra
AU  - Djekić, Ivana
AU  - Ristić, Danijela
AU  - Berenji, Janoš
AU  - Krstić, Branka
PY  - 2009
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1953
AB  - Zucchini yellow mosaic virus (ZYMV) has become an important pathogen of cucurbits over the past 20 years. In our country, this virus also causes significant losses, especially in oil pumpkin production. Although ZYMV variability has been extensively studied worldwide by biological and molecular characterization, such information is still limited for the European region. Herein, we present the first analysis of phenotypic variations of ZYMV isolates sampled from oil pumpkin cv. Olinka from different locations in Serbia during 2008. Twelve isolates were obtained by mechanical inoculation on the C. pepo hybrid Ezra F1, and four of them were chosen for further detailed biological study. These investigated isolates exhibited different phenotypical features and thus represented different variants, concerning their host range, and type and severity of symptoms. A previously developed primer pair, ZYMV CPfwd/CPrev, was tested to determine its suitability for detection of Serbian ZYMV isolates. Amplification of Serbian ZYMV isolates of different origin, in single and mixed infection, resulted in one distinctive band. The used primer pair proved to be useful not only for routine molecular detection, but also for further molecular characterization of Serbian ZYMV isolates because it amplifies the hypervariable genomic region of ZYMV. The result obtained in this study is a contribution to investigation of genetic diversity of Serbian ZYMV isolates and their molecular relationship with ZYMV isolates from other parts of the world.
AB  - Poslednjih 20 godina virus žutog mozaika cukinija (Zucchini yellow mosaic virus, ZYMV) se javlja kao značajan patogen biljaka iz familije tikava. Kod nas ovaj virus, takođe, izaziva značajne gubitke, naročito u proizvodnji uljane tikve. Iako je biološka i molekularna varijabilnost ZYMV dosta ispitivana, još uvek nema dovoljno informacija o varijabilnosti evropskih izolata ovog virusa. Ovaj rad predstavlja prvu analizu fenotipske varijabilnosti izolata ZYMV poreklom iz Srbije, koji su tokom 2008. godine sakupljeni iz različitih lokaliteta gajenja uljane tikve sorte Olinka. Mehaničkim inokulacijama C. pepo hibrid Ezra F1, dobijeno je 12 izolata, od kojih su četiri odabrana za dalju biološku karakterizaciju. Na osnovu različitog kruga domaćina, tipa i jačine simptoma na test-biljkama, utvrđena je fenotipska varijabilnost ispitivanih izolata. Par prajmera ZYMV CPfwd/Cprev testiran je radi utvrđivanja pogodnosti za rutinsku detekciju izolata ZYMV poreklom iz Srbije. Na osnovu pojave traka očekivane veličine u gelu, ZYMV je detektovan i u pojedinačnim i mešanim infekcijama u biljkama sa različitih lokaliteta, što dokazuje pogodnost ovih prajmera. Kako ovi prajmeri amplifikuju hipervarijabilni deo genoma virusa, pogodni su ne samo za rutinsku molekularnu detekciju, već i za molekularnu karakterizaciju izolata ZYMV iz Srbije. Rezultati dobijeni u ovom radu omogućiće dalja proučavanja genetičke varijabilnosti izolata ZYMV iz Srbije, kao i njihove evolutivne povezanosti sa izolatima iz drugih delova sveta.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Biological variability of zucchini yellow mosaic virus in Serbia
T1  - Biološka varijabilnost virusa žutog mozaika cukinija u Srbiji
EP  - 280
IS  - 4
SP  - 271
VL  - 24
DO  - 10.2298/PIF0904271V
ER  - 

@article{
author = "Vučurović, Ana and Bulajić, Aleksandra and Djekić, Ivana and Ristić, Danijela and Berenji, Janoš and Krstić, Branka",
year = "2009",
abstract = "Zucchini yellow mosaic virus (ZYMV) has become an important pathogen of cucurbits over the past 20 years. In our country, this virus also causes significant losses, especially in oil pumpkin production. Although ZYMV variability has been extensively studied worldwide by biological and molecular characterization, such information is still limited for the European region. Herein, we present the first analysis of phenotypic variations of ZYMV isolates sampled from oil pumpkin cv. Olinka from different locations in Serbia during 2008. Twelve isolates were obtained by mechanical inoculation on the C. pepo hybrid Ezra F1, and four of them were chosen for further detailed biological study. These investigated isolates exhibited different phenotypical features and thus represented different variants, concerning their host range, and type and severity of symptoms. A previously developed primer pair, ZYMV CPfwd/CPrev, was tested to determine its suitability for detection of Serbian ZYMV isolates. Amplification of Serbian ZYMV isolates of different origin, in single and mixed infection, resulted in one distinctive band. The used primer pair proved to be useful not only for routine molecular detection, but also for further molecular characterization of Serbian ZYMV isolates because it amplifies the hypervariable genomic region of ZYMV. The result obtained in this study is a contribution to investigation of genetic diversity of Serbian ZYMV isolates and their molecular relationship with ZYMV isolates from other parts of the world., Poslednjih 20 godina virus žutog mozaika cukinija (Zucchini yellow mosaic virus, ZYMV) se javlja kao značajan patogen biljaka iz familije tikava. Kod nas ovaj virus, takođe, izaziva značajne gubitke, naročito u proizvodnji uljane tikve. Iako je biološka i molekularna varijabilnost ZYMV dosta ispitivana, još uvek nema dovoljno informacija o varijabilnosti evropskih izolata ovog virusa. Ovaj rad predstavlja prvu analizu fenotipske varijabilnosti izolata ZYMV poreklom iz Srbije, koji su tokom 2008. godine sakupljeni iz različitih lokaliteta gajenja uljane tikve sorte Olinka. Mehaničkim inokulacijama C. pepo hibrid Ezra F1, dobijeno je 12 izolata, od kojih su četiri odabrana za dalju biološku karakterizaciju. Na osnovu različitog kruga domaćina, tipa i jačine simptoma na test-biljkama, utvrđena je fenotipska varijabilnost ispitivanih izolata. Par prajmera ZYMV CPfwd/Cprev testiran je radi utvrđivanja pogodnosti za rutinsku detekciju izolata ZYMV poreklom iz Srbije. Na osnovu pojave traka očekivane veličine u gelu, ZYMV je detektovan i u pojedinačnim i mešanim infekcijama u biljkama sa različitih lokaliteta, što dokazuje pogodnost ovih prajmera. Kako ovi prajmeri amplifikuju hipervarijabilni deo genoma virusa, pogodni su ne samo za rutinsku molekularnu detekciju, već i za molekularnu karakterizaciju izolata ZYMV iz Srbije. Rezultati dobijeni u ovom radu omogućiće dalja proučavanja genetičke varijabilnosti izolata ZYMV iz Srbije, kao i njihove evolutivne povezanosti sa izolatima iz drugih delova sveta.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Biological variability of zucchini yellow mosaic virus in Serbia, Biološka varijabilnost virusa žutog mozaika cukinija u Srbiji",
pages = "280-271",
number = "4",
volume = "24",
doi = "10.2298/PIF0904271V"
}

Vučurović, A., Bulajić, A., Djekić, I., Ristić, D., Berenji, J.,& Krstić, B.. (2009). Biological variability of zucchini yellow mosaic virus in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 24(4), 271-280.
https://doi.org/10.2298/PIF0904271V

Vučurović A, Bulajić A, Djekić I, Ristić D, Berenji J, Krstić B. Biological variability of zucchini yellow mosaic virus in Serbia. in Pesticidi i fitomedicina. 2009;24(4):271-280.
doi:10.2298/PIF0904271V .

Vučurović, Ana, Bulajić, Aleksandra, Djekić, Ivana, Ristić, Danijela, Berenji, Janoš, Krstić, Branka, "Biological variability of zucchini yellow mosaic virus in Serbia" in Pesticidi i fitomedicina, 24, no. 4 (2009):271-280,
https://doi.org/10.2298/PIF0904271V . .

TY  - JOUR
AU  - Vučurović, Ana
AU  - Bulajić, Aleksandra
AU  - Djekić, Ivana
AU  - Ristić, Danijela
AU  - Berenji, Janoš
AU  - Krstić, Branka
PY  - 2009
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1952
AB  - Over the past decade, intensive spread of virus infections of oilseed pumpkin has resulted in significant economic losses in pumpkin crop production, which is currently expanding in our country. In 2007 and 2008, a survey for the presence and distribution of oilseed pumpkin viruses was carried out in order to identify viruses responsible for epidemics and incidences of very destructive symptoms on cucurbit leaves and fruits. Monitoring and collecting samples of oil pumpkin, as well as other species such as winter and butternut squash and buffalo and bottle gourd with viral infection symptoms, was conducted in several localities of Vojvodina Province. The collected plant samples were tested by DAS-ELISA using polyclonal antisera specific for the detection of six most economically harmful pumpkin viruses: Cucumber mosaic virus (CMV), Zucchini yellow mosaic virus (ZYMV), Watermelon mosaic virus (WMW), Squash mosaic virus (SqMV), Papaya ringspot virus (PRSV) and Tobacco ringspot virus (TRSV) that are included in A1 quarantine list of harmful organisms in Serbia. Identification of viruses in the collected samples indicated the presence of three viruses, ZYMV, WMV and CMV, in individual and mixed infections. Frequency of the identified viruses varied depending on locality and year of investigations. In 2007, WMV was the most frequent virus (94.2%), while ZYMV was prevalent (98.04%) in 2008. High frequency of ZYMV determined in both years of investigation indicated the need for its rapid and reliable molecular detection. During this investigation, a protocol for ZYMV detection was developed and optimized using specific primers CPfwd/Cprev and commercial kits for total RNA extraction, as well as for RT-PCR. In RT-PCR reaction using these primers, a DNA fragment of approximately 1100 bp, which included coat protein gene, was amplified in the samples of infected pumkin leaves. Although serological methods are still useful for large-scale testing of a great number of samples, this protocol, due to its high sensitivity and specificity, is an important improvement in rapid diagnosis of diseases caused by this virus. In addition, the protocol provides a basis for further characterization of ZYMV isolates originating from Serbia.
AB  - Intenzivno širenje virusa infektivnih za uljanu tikvu (Cucurbita pepo), poslednjih deset godina imalo je za posledicu značajne ekonomske gubitke u proizvodnji ove kulture koja se gaji na sve većim površinama u našoj zemlji. Kako bi se identifikovali virusi, odgovorni za epidemijsku pojavu i ispoljavanje veoma destruktivnih simptoma na lišću i plodovima uljane tikve, tokom 2007. i 2008. sprovedeno je ispitivanje njihove pojave i rasprostranjenosti. Pregled i sakupljanje uzoraka uljane tikve, kao i nekih drugih vrsta tikava, kao što su bundeva, muskatna i bizonska tikva i vrg sa simptomima virusnih zaraza, obavljeno je na više različitih lokaliteta gajenja uljane tikve u Vojvodini. Sakupljeni uzorci testirani su DAS-ELISA metodom primenom poliklonalnih antiseruma specifičnih za detekciju u svetu šest ekonomski najznačajnijih virusa tikava: virusa mozaika krastavca (Cucumber mosaic virus, CMV), virusa žutog mozaika cukinija (Zucchini yellow mosaic virus, ZYMV), virusa mozaika lubenice (Watermelon mosaic virus, WMW), virusa mozaika bundeve (Squash mosaic virus, SqMV), virusa prstenaste pegavosti papaje (Papaya ringspot virus, PRSV) i virusa prstenaste pegavosti duvana (Tobacco ringspot virus, TRSV), koji se nalazi na A1 karantinskoj listi štetnih organizama u Srbiji. Identifikacija virusa u sakupljenim uzorcima ukazala je na prisustvo tri virusa, ZYMV, WMV i CMV, koji su se javili u pojedinačnim ili mešanim infekcijama. Njihova učestalost je po pojedinim godinama i lokalitetima bila različita. Tokom 2007. najčešće je dokazan WMV (94,2%), dok je 2008. godine prevalentan virus bio ZYMV (98,04%). Velika učestalost ZYMV u obe godine ispitivanja ukazala je na potrebu za brzom i pouzdanom molekularnom detekcijom ovog virusa. U toku ovih istraživanja razvijen je i optimizovan protokol za detekciju ZYMV primenom specifičnih prajmera CPfwd/CPrev i komercijalnih kitova za ekstrakciju ukupne RNA i RT-PCR. Korišćenjem ovih prajmera, kojim se amplifikuje deo genoma ZYMV kojim je obuhvaćen i gen za proteinski omotač, umnožen je DNK fragment dužine oko 1100 bp iz lišća zaraženih biljaka. Mada serološke metode i dalje imaju veliku prednost u primeni za masovna testiranja velikog broja uzoraka, razvijeni protokol molekularne detekcije, zbog visoke osetljivosti i specifičnosti predstavlja značajno poboljšanje brze dijagnoze oboljenja koja ovaj virus izaziva. Osim toga, ovaj protokol pruža osnovu za dalju karakterizaciju ZYMV izolata poreklom iz Srbije.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Presence and distribution of oilseed pumpkin viruses and molecular detection of Zucchini yellow mosaic virus
T1  - Prisustvo i rasprostranjenost virusa uljane tikve i molekularna detekcija virusa žutog mozaika cukinija
EP  - 94
IS  - 2
SP  - 85
VL  - 24
DO  - 10.2298/PIF0902085V
ER  - 

@article{
author = "Vučurović, Ana and Bulajić, Aleksandra and Djekić, Ivana and Ristić, Danijela and Berenji, Janoš and Krstić, Branka",
year = "2009",
abstract = "Over the past decade, intensive spread of virus infections of oilseed pumpkin has resulted in significant economic losses in pumpkin crop production, which is currently expanding in our country. In 2007 and 2008, a survey for the presence and distribution of oilseed pumpkin viruses was carried out in order to identify viruses responsible for epidemics and incidences of very destructive symptoms on cucurbit leaves and fruits. Monitoring and collecting samples of oil pumpkin, as well as other species such as winter and butternut squash and buffalo and bottle gourd with viral infection symptoms, was conducted in several localities of Vojvodina Province. The collected plant samples were tested by DAS-ELISA using polyclonal antisera specific for the detection of six most economically harmful pumpkin viruses: Cucumber mosaic virus (CMV), Zucchini yellow mosaic virus (ZYMV), Watermelon mosaic virus (WMW), Squash mosaic virus (SqMV), Papaya ringspot virus (PRSV) and Tobacco ringspot virus (TRSV) that are included in A1 quarantine list of harmful organisms in Serbia. Identification of viruses in the collected samples indicated the presence of three viruses, ZYMV, WMV and CMV, in individual and mixed infections. Frequency of the identified viruses varied depending on locality and year of investigations. In 2007, WMV was the most frequent virus (94.2%), while ZYMV was prevalent (98.04%) in 2008. High frequency of ZYMV determined in both years of investigation indicated the need for its rapid and reliable molecular detection. During this investigation, a protocol for ZYMV detection was developed and optimized using specific primers CPfwd/Cprev and commercial kits for total RNA extraction, as well as for RT-PCR. In RT-PCR reaction using these primers, a DNA fragment of approximately 1100 bp, which included coat protein gene, was amplified in the samples of infected pumkin leaves. Although serological methods are still useful for large-scale testing of a great number of samples, this protocol, due to its high sensitivity and specificity, is an important improvement in rapid diagnosis of diseases caused by this virus. In addition, the protocol provides a basis for further characterization of ZYMV isolates originating from Serbia., Intenzivno širenje virusa infektivnih za uljanu tikvu (Cucurbita pepo), poslednjih deset godina imalo je za posledicu značajne ekonomske gubitke u proizvodnji ove kulture koja se gaji na sve većim površinama u našoj zemlji. Kako bi se identifikovali virusi, odgovorni za epidemijsku pojavu i ispoljavanje veoma destruktivnih simptoma na lišću i plodovima uljane tikve, tokom 2007. i 2008. sprovedeno je ispitivanje njihove pojave i rasprostranjenosti. Pregled i sakupljanje uzoraka uljane tikve, kao i nekih drugih vrsta tikava, kao što su bundeva, muskatna i bizonska tikva i vrg sa simptomima virusnih zaraza, obavljeno je na više različitih lokaliteta gajenja uljane tikve u Vojvodini. Sakupljeni uzorci testirani su DAS-ELISA metodom primenom poliklonalnih antiseruma specifičnih za detekciju u svetu šest ekonomski najznačajnijih virusa tikava: virusa mozaika krastavca (Cucumber mosaic virus, CMV), virusa žutog mozaika cukinija (Zucchini yellow mosaic virus, ZYMV), virusa mozaika lubenice (Watermelon mosaic virus, WMW), virusa mozaika bundeve (Squash mosaic virus, SqMV), virusa prstenaste pegavosti papaje (Papaya ringspot virus, PRSV) i virusa prstenaste pegavosti duvana (Tobacco ringspot virus, TRSV), koji se nalazi na A1 karantinskoj listi štetnih organizama u Srbiji. Identifikacija virusa u sakupljenim uzorcima ukazala je na prisustvo tri virusa, ZYMV, WMV i CMV, koji su se javili u pojedinačnim ili mešanim infekcijama. Njihova učestalost je po pojedinim godinama i lokalitetima bila različita. Tokom 2007. najčešće je dokazan WMV (94,2%), dok je 2008. godine prevalentan virus bio ZYMV (98,04%). Velika učestalost ZYMV u obe godine ispitivanja ukazala je na potrebu za brzom i pouzdanom molekularnom detekcijom ovog virusa. U toku ovih istraživanja razvijen je i optimizovan protokol za detekciju ZYMV primenom specifičnih prajmera CPfwd/CPrev i komercijalnih kitova za ekstrakciju ukupne RNA i RT-PCR. Korišćenjem ovih prajmera, kojim se amplifikuje deo genoma ZYMV kojim je obuhvaćen i gen za proteinski omotač, umnožen je DNK fragment dužine oko 1100 bp iz lišća zaraženih biljaka. Mada serološke metode i dalje imaju veliku prednost u primeni za masovna testiranja velikog broja uzoraka, razvijeni protokol molekularne detekcije, zbog visoke osetljivosti i specifičnosti predstavlja značajno poboljšanje brze dijagnoze oboljenja koja ovaj virus izaziva. Osim toga, ovaj protokol pruža osnovu za dalju karakterizaciju ZYMV izolata poreklom iz Srbije.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Presence and distribution of oilseed pumpkin viruses and molecular detection of Zucchini yellow mosaic virus, Prisustvo i rasprostranjenost virusa uljane tikve i molekularna detekcija virusa žutog mozaika cukinija",
pages = "94-85",
number = "2",
volume = "24",
doi = "10.2298/PIF0902085V"
}

Vučurović, A., Bulajić, A., Djekić, I., Ristić, D., Berenji, J.,& Krstić, B.. (2009). Presence and distribution of oilseed pumpkin viruses and molecular detection of Zucchini yellow mosaic virus. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 24(2), 85-94.
https://doi.org/10.2298/PIF0902085V

Vučurović A, Bulajić A, Djekić I, Ristić D, Berenji J, Krstić B. Presence and distribution of oilseed pumpkin viruses and molecular detection of Zucchini yellow mosaic virus. in Pesticidi i fitomedicina. 2009;24(2):85-94.
doi:10.2298/PIF0902085V .

Vučurović, Ana, Bulajić, Aleksandra, Djekić, Ivana, Ristić, Danijela, Berenji, Janoš, Krstić, Branka, "Presence and distribution of oilseed pumpkin viruses and molecular detection of Zucchini yellow mosaic virus" in Pesticidi i fitomedicina, 24, no. 2 (2009):85-94,
https://doi.org/10.2298/PIF0902085V . .

TY  - JOUR
AU  - Djekić, Ivana
AU  - Bulajić, Aleksandra
AU  - Vučurović, Ana
AU  - Ristić, Danijela
AU  - Krstić, Branka
PY  - 2008
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1670
AB  - Reliable detection of plant pathogens does not only mean the development of sufficiently sensitive laboratory techniques for their routine testing. Regardless of the sensitivity of applied methods, the proper selection of samples to be tested has crucial influence on method reliability. Due to uneven distribution of Tomato spotted wilt virus (TSWV) in naturally infected systemic host plants, the collection and sampling of material for assaying is a critical moment upon which the reliability of laboratory procedure depends. The effect of irregular virus distribution on its serological detection was examined in tomato, pepper and four ornamental species, as its most important host plants in our country. The reliability of virus detection, depending on its uneven distribution, was assessed by serological testing of tomato and pepper symptomatic leaves and fruits, and symptomatic and asymptomatic young and old leaves, as well as flower petals of ornamentals. Although TSWV was detected using ELISA in the majority of plants included in the experiment, the tests indicated an uneven distribution and unequal concentrations of TSWV in different parts of the plants. The virus could not be detected in a certain number of subsamples, prepared from infected tomato and pepper fruits and older ornamental leaves. The virus also could not be detected in some ornamentals and tomato plants with intensive symptoms. Conversely, the virus was detected in three ornamental plants without any symptoms. Examining the virus distribution in different plant parts indicated that the reliability of ELISA could be reached not only by sampling younger ornamental leaves, but also by preparing compound samples with as much leaves as possible, or by testing a greater number of subsamples of the tested plant. Considering a small possibility of TSWV detection in tomato and pepper fruits, the infection of these should be established by testing their leaves. Besides, the data show that negative test results for the known host plants with characteristic symptoms require tests to be repeated using more sensitive methods than ELISA. These results are important as guidelines for growers and professionals who submit samples, as well as for laboratories performing routine testing.
AB  - Pouzdana detekcija fitopatogenih organizama ne podrazumeva samo razvijanje dovoljno osetljivih laboratorijskih tehnika za rutinsko testiranje. Bez obzira koliko primenjene metode bile osetljive, na njihovu pouzdanost presudno utiče pravilan izbor uzoraka koji će se testirati. Za virus bronzavosti paradajza (Tomato spotted wilt virus, TSWV), zbog neravnomerne distribucije u sistemično zaraženim biljkama domaćinima, prikupljanje i priprema uzoraka za testiranje predstavljaju kritični momenat od koga zavisi pouzdanost laboratorijske metode. Sprovedena su ispitivanja uticaja neravnomerne distribucije virusa u paradajzu, paprici i četiri vrste ukrasnih biljaka, kao najznačajnijim domaćinima ovog virusa u našoj zemlji. Pouzdanost detekcije virusa, u zavisnosti od njegove neravnomerne distribucije, utvrđivana je serološkim testiranjem lišća i plodova paradajza i paprike sa simptomima i simptomatičnog i asimptomatičnog mlađeg i starijeg lišća, kao i kruničnih listića ukrasnih biljaka. Iako je primenom ELISA metode TSWV dokazan u većini biljaka uključenih u eksperiment, obavljena testiranja ukazala su na neravnomernu distribuciju i različitu koncentraciju TSWV u različitim delovima biljaka. Prisustvo virusa nije moglo biti dokazano u određenom broju poduzoraka pripremljenih od zaraženih biljaka, plodova paradajza i paprike i starijeg lišća ukrasnih biljaka. Virus nije mogao biti detektovan ni u nekim ukrasnim biljkama i biljkama paradajza sa izraženim simptomima. Nasuprot tome, TSWV je dokazan u tri ukrasne biljke bez simptoma. Ispitivanja distribucije virusa u biljci ukazala su da se pouzdanost ELISA metode može postići uzorkovanjem ne samo mlađeg lišća ukrasnih biljaka, već pripremom zbirnog uzorka sa što više listova ili ispitivanjem većeg broja poduzoraka biljke koja se testira. S obzirom na malu mogućnost detekcije TSWV u plodovima paradajza i paprike, utvrđivanje zaraženosti ovih biljaka treba obaviti testiranjem lišća. Pored toga, dobijeni rezultati ukazuju da je u slučaju pojave negativnih rezultata testiranjem uzoraka sa karakterističnim simptomima na poznatim domaćinima, potrebno ponoviti testiranje osetljivijim metodama od ELISA. Dobijeni rezultati značajni su kao smernica za proizvođače i stručna lica koja dostavljaju uzorke i laboratorije zadužene za rutinska testiranja.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Influence of Tomato spotted wilt virus uneven distribution on its serological detection in tomato, pepper and ornamentals
T1  - Uticaj neravnomerne distribucije virusa bronzavosti paradajza na serološko dokazivanje virusa u paradajzu, paprici i ukrasnim biljkama
EP  - 234
IS  - 4
SP  - 225
VL  - 23
DO  - 10.2298/PIF0804225D
ER  - 

@article{
author = "Djekić, Ivana and Bulajić, Aleksandra and Vučurović, Ana and Ristić, Danijela and Krstić, Branka",
year = "2008",
abstract = "Reliable detection of plant pathogens does not only mean the development of sufficiently sensitive laboratory techniques for their routine testing. Regardless of the sensitivity of applied methods, the proper selection of samples to be tested has crucial influence on method reliability. Due to uneven distribution of Tomato spotted wilt virus (TSWV) in naturally infected systemic host plants, the collection and sampling of material for assaying is a critical moment upon which the reliability of laboratory procedure depends. The effect of irregular virus distribution on its serological detection was examined in tomato, pepper and four ornamental species, as its most important host plants in our country. The reliability of virus detection, depending on its uneven distribution, was assessed by serological testing of tomato and pepper symptomatic leaves and fruits, and symptomatic and asymptomatic young and old leaves, as well as flower petals of ornamentals. Although TSWV was detected using ELISA in the majority of plants included in the experiment, the tests indicated an uneven distribution and unequal concentrations of TSWV in different parts of the plants. The virus could not be detected in a certain number of subsamples, prepared from infected tomato and pepper fruits and older ornamental leaves. The virus also could not be detected in some ornamentals and tomato plants with intensive symptoms. Conversely, the virus was detected in three ornamental plants without any symptoms. Examining the virus distribution in different plant parts indicated that the reliability of ELISA could be reached not only by sampling younger ornamental leaves, but also by preparing compound samples with as much leaves as possible, or by testing a greater number of subsamples of the tested plant. Considering a small possibility of TSWV detection in tomato and pepper fruits, the infection of these should be established by testing their leaves. Besides, the data show that negative test results for the known host plants with characteristic symptoms require tests to be repeated using more sensitive methods than ELISA. These results are important as guidelines for growers and professionals who submit samples, as well as for laboratories performing routine testing., Pouzdana detekcija fitopatogenih organizama ne podrazumeva samo razvijanje dovoljno osetljivih laboratorijskih tehnika za rutinsko testiranje. Bez obzira koliko primenjene metode bile osetljive, na njihovu pouzdanost presudno utiče pravilan izbor uzoraka koji će se testirati. Za virus bronzavosti paradajza (Tomato spotted wilt virus, TSWV), zbog neravnomerne distribucije u sistemično zaraženim biljkama domaćinima, prikupljanje i priprema uzoraka za testiranje predstavljaju kritični momenat od koga zavisi pouzdanost laboratorijske metode. Sprovedena su ispitivanja uticaja neravnomerne distribucije virusa u paradajzu, paprici i četiri vrste ukrasnih biljaka, kao najznačajnijim domaćinima ovog virusa u našoj zemlji. Pouzdanost detekcije virusa, u zavisnosti od njegove neravnomerne distribucije, utvrđivana je serološkim testiranjem lišća i plodova paradajza i paprike sa simptomima i simptomatičnog i asimptomatičnog mlađeg i starijeg lišća, kao i kruničnih listića ukrasnih biljaka. Iako je primenom ELISA metode TSWV dokazan u većini biljaka uključenih u eksperiment, obavljena testiranja ukazala su na neravnomernu distribuciju i različitu koncentraciju TSWV u različitim delovima biljaka. Prisustvo virusa nije moglo biti dokazano u određenom broju poduzoraka pripremljenih od zaraženih biljaka, plodova paradajza i paprike i starijeg lišća ukrasnih biljaka. Virus nije mogao biti detektovan ni u nekim ukrasnim biljkama i biljkama paradajza sa izraženim simptomima. Nasuprot tome, TSWV je dokazan u tri ukrasne biljke bez simptoma. Ispitivanja distribucije virusa u biljci ukazala su da se pouzdanost ELISA metode može postići uzorkovanjem ne samo mlađeg lišća ukrasnih biljaka, već pripremom zbirnog uzorka sa što više listova ili ispitivanjem većeg broja poduzoraka biljke koja se testira. S obzirom na malu mogućnost detekcije TSWV u plodovima paradajza i paprike, utvrđivanje zaraženosti ovih biljaka treba obaviti testiranjem lišća. Pored toga, dobijeni rezultati ukazuju da je u slučaju pojave negativnih rezultata testiranjem uzoraka sa karakterističnim simptomima na poznatim domaćinima, potrebno ponoviti testiranje osetljivijim metodama od ELISA. Dobijeni rezultati značajni su kao smernica za proizvođače i stručna lica koja dostavljaju uzorke i laboratorije zadužene za rutinska testiranja.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Influence of Tomato spotted wilt virus uneven distribution on its serological detection in tomato, pepper and ornamentals, Uticaj neravnomerne distribucije virusa bronzavosti paradajza na serološko dokazivanje virusa u paradajzu, paprici i ukrasnim biljkama",
pages = "234-225",
number = "4",
volume = "23",
doi = "10.2298/PIF0804225D"
}

Djekić, I., Bulajić, A., Vučurović, A., Ristić, D.,& Krstić, B.. (2008). Influence of Tomato spotted wilt virus uneven distribution on its serological detection in tomato, pepper and ornamentals. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 23(4), 225-234.
https://doi.org/10.2298/PIF0804225D

Djekić I, Bulajić A, Vučurović A, Ristić D, Krstić B. Influence of Tomato spotted wilt virus uneven distribution on its serological detection in tomato, pepper and ornamentals. in Pesticidi i fitomedicina. 2008;23(4):225-234.
doi:10.2298/PIF0804225D .

Djekić, Ivana, Bulajić, Aleksandra, Vučurović, Ana, Ristić, Danijela, Krstić, Branka, "Influence of Tomato spotted wilt virus uneven distribution on its serological detection in tomato, pepper and ornamentals" in Pesticidi i fitomedicina, 23, no. 4 (2008):225-234,
https://doi.org/10.2298/PIF0804225D . .