TY  - JOUR
AU  - Savić, Slađana
AU  - Belić, Lela
AU  - Marjanović, Milena
AU  - Radović, Ivana
AU  - Girek, Zdenka
AU  - Zečević, Veselinka
AU  - Jovanović, Zorica
PY  - 2024
UR  - http://www.ifrj.upm.edu.my/31%20(01)%202024/08%20-%20IFRJ23068.R1.pdf
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6886
AB  - Tomato, one of the most produced vegetables in the world, is experiencing continuous
global increase in both production and consumption. Fruit quality traits are important for
fresh market tomatoes as well as for the processing industry. Despite the growing demand
for both fresh and processed tomatoes, consumers are not satisfied with the quality of
available fruits. The main objectives of the present work were to determine the
physicochemical characteristics [pH, total soluble solids (TSS), total titratable acids
(TTA), TSS/TTA ratio, DMC, lycopene, β-carotene, vitamin C, and total phenolic
content], as well as the antioxidant activity of 13 different tomato lines, and to identify the
most promising ones in terms of fruit taste and quality. Antioxidant activity was
determined using the ABTS and DPPH methods with Trolox used as the standard
compound. PCA analysis was conducted to identify group patterns. The results of PCA
analysis indicated a specific genotypic response in all investigated physicochemical traits.
Genotypes 2, 10, and 13 were identified as the best for fresh consumption, as they
exhibited the highest levels of compounds crucial for good taste, nutrition, and human
health benefits. The most promising genotype related to fruit quality attributes was
genotype 10 with the best TSS and TAA content and TSS/TAA ratio, which is important
for overall taste perception. On the other hand, genotype 9 showed promise for industrial
purposes due to its ideal pH value in the juice and good soluble solid content. High
antioxidant activity was characteristic of genotypes 1 and 2, and their consumption as fresh
tomatoes can be beneficial to human health. They also should be considered for further
evaluation as potentially interesting genotypes for abiotic stress research and selection
programs which can lead to the development of both superior fruit quality and stress
tolerant genotypes.
PB  - Universiti Putra Malaysia
T2  - International Food Research Journal 31(1): 87 - 97 (February 2024)
T1  - Determination of bioactive components in different tomato lines: Physicochemical properties and antioxidant activity
EP  - 97
IS  - 31
SP  - 87
VL  - 1
DO  - 10.47836/ifrj.31.1.08
ER  - 

@article{
author = "Savić, Slađana and Belić, Lela and Marjanović, Milena and Radović, Ivana and Girek, Zdenka and Zečević, Veselinka and Jovanović, Zorica",
year = "2024",
abstract = "Tomato, one of the most produced vegetables in the world, is experiencing continuous
global increase in both production and consumption. Fruit quality traits are important for
fresh market tomatoes as well as for the processing industry. Despite the growing demand
for both fresh and processed tomatoes, consumers are not satisfied with the quality of
available fruits. The main objectives of the present work were to determine the
physicochemical characteristics [pH, total soluble solids (TSS), total titratable acids
(TTA), TSS/TTA ratio, DMC, lycopene, β-carotene, vitamin C, and total phenolic
content], as well as the antioxidant activity of 13 different tomato lines, and to identify the
most promising ones in terms of fruit taste and quality. Antioxidant activity was
determined using the ABTS and DPPH methods with Trolox used as the standard
compound. PCA analysis was conducted to identify group patterns. The results of PCA
analysis indicated a specific genotypic response in all investigated physicochemical traits.
Genotypes 2, 10, and 13 were identified as the best for fresh consumption, as they
exhibited the highest levels of compounds crucial for good taste, nutrition, and human
health benefits. The most promising genotype related to fruit quality attributes was
genotype 10 with the best TSS and TAA content and TSS/TAA ratio, which is important
for overall taste perception. On the other hand, genotype 9 showed promise for industrial
purposes due to its ideal pH value in the juice and good soluble solid content. High
antioxidant activity was characteristic of genotypes 1 and 2, and their consumption as fresh
tomatoes can be beneficial to human health. They also should be considered for further
evaluation as potentially interesting genotypes for abiotic stress research and selection
programs which can lead to the development of both superior fruit quality and stress
tolerant genotypes.",
publisher = "Universiti Putra Malaysia",
journal = "International Food Research Journal 31(1): 87 - 97 (February 2024)",
title = "Determination of bioactive components in different tomato lines: Physicochemical properties and antioxidant activity",
pages = "97-87",
number = "31",
volume = "1",
doi = "10.47836/ifrj.31.1.08"
}

Savić, S., Belić, L., Marjanović, M., Radović, I., Girek, Z., Zečević, V.,& Jovanović, Z.. (2024). Determination of bioactive components in different tomato lines: Physicochemical properties and antioxidant activity. in International Food Research Journal 31(1): 87 - 97 (February 2024)
Universiti Putra Malaysia., 1(31), 87-97.
https://doi.org/10.47836/ifrj.31.1.08

Savić S, Belić L, Marjanović M, Radović I, Girek Z, Zečević V, Jovanović Z. Determination of bioactive components in different tomato lines: Physicochemical properties and antioxidant activity. in International Food Research Journal 31(1): 87 - 97 (February 2024). 2024;1(31):87-97.
doi:10.47836/ifrj.31.1.08 .

Savić, Slađana, Belić, Lela, Marjanović, Milena, Radović, Ivana, Girek, Zdenka, Zečević, Veselinka, Jovanović, Zorica, "Determination of bioactive components in different tomato lines: Physicochemical properties and antioxidant activity" in International Food Research Journal 31(1): 87 - 97 (February 2024), 1, no. 31 (2024):87-97,
https://doi.org/10.47836/ifrj.31.1.08 . .

TY  - JOUR
AU  - Urošević, Dušan
AU  - Knežević, Desimir
AU  - Branković, Gordana
AU  - Novoselskaya-Dragovich, Aleksandra Yu.
AU  - Kudryavtsev, Alexander M.
AU  - Stojšin, Mirela M.
AU  - Mićanović, Danica
AU  - Zečević, Veselinka
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6365
AB  - The aim of this study was determination of protein content and amino acid composition in seeds of bread wheat, with particular focus of evaluation essential amino acids (EAAs). For analysis used flour samples of grained seed of 10 wheat variety, which selected in different breeding center (in Novi Sad and Kragujevac, Serbia). Kjeldahl method was used for determination of nitrogen (N) contents which value multiplied with coefficient 5.7 for computing protein content (protein contents = 5.7 x % N contents). Amino acids analyses of wheat samples were performed by ion exchange chromatography, followed by the ninhydrin colour reaction and photometric detection at 570 nm and 440 nm (for proline). The results showed that the mean protein content for wheat varied from the lowest value 10.24% in Ljubičevka to the highest 14.21% in Fortuna variety. The mean contents (g 100 g-l protein) of nonessential amino acids (NEAAs) were aspartic acid 5.42%, serine 4.23%, glutamic acid 18.51%, proline 12.18%, glycine 4.17%, alanine 3.64%, tyrosine 2.52%, arginine 5.02%, while mean contents of essential amino acids (EAAs) were threonine 2.86%, valine 4.52%, methionine 1.28%, isoleucine 3.87%, leucine 5.87%, phenylalanine 4.62%, histidine 2.46%, lysine 2.91%. The wheat variety contained relatively high concentration of the most deficient EAAs. The variety Ljubičevka contained relatively high concentration of the most deficient EAAs (threonine, valine, leucine, lysine,). The highest value (29.31 g 100 g-l protein) of total essential amino acids (TEAA), 59.79 g 100 g-l protein of total nonessential amino acids (TNEAA) as well as the highest value (90.35 g 100 g-l protein) of total amino acids (TAA). Obtained results provide variability of wheat variety on the base of amino acid contents and indicate reliable variety for selecting desirable parents in breeding program for improving nutrient quality © 2023, Genetika.All Rights Reserved.
T2  - Genetika
T2  - Genetika
T1  - PROTEIN CONTENT AND AMINO ACID COMPOSITION IN SEED OF BREAD WHEAT (Triticum aestivum L.)
EP  - 318
IS  - 1
SP  - 301
VL  - 55
DO  - 10.2298/GENSR23010301U
ER  - 

@article{
author = "Urošević, Dušan and Knežević, Desimir and Branković, Gordana and Novoselskaya-Dragovich, Aleksandra Yu. and Kudryavtsev, Alexander M. and Stojšin, Mirela M. and Mićanović, Danica and Zečević, Veselinka",
year = "2023",
abstract = "The aim of this study was determination of protein content and amino acid composition in seeds of bread wheat, with particular focus of evaluation essential amino acids (EAAs). For analysis used flour samples of grained seed of 10 wheat variety, which selected in different breeding center (in Novi Sad and Kragujevac, Serbia). Kjeldahl method was used for determination of nitrogen (N) contents which value multiplied with coefficient 5.7 for computing protein content (protein contents = 5.7 x % N contents). Amino acids analyses of wheat samples were performed by ion exchange chromatography, followed by the ninhydrin colour reaction and photometric detection at 570 nm and 440 nm (for proline). The results showed that the mean protein content for wheat varied from the lowest value 10.24% in Ljubičevka to the highest 14.21% in Fortuna variety. The mean contents (g 100 g-l protein) of nonessential amino acids (NEAAs) were aspartic acid 5.42%, serine 4.23%, glutamic acid 18.51%, proline 12.18%, glycine 4.17%, alanine 3.64%, tyrosine 2.52%, arginine 5.02%, while mean contents of essential amino acids (EAAs) were threonine 2.86%, valine 4.52%, methionine 1.28%, isoleucine 3.87%, leucine 5.87%, phenylalanine 4.62%, histidine 2.46%, lysine 2.91%. The wheat variety contained relatively high concentration of the most deficient EAAs. The variety Ljubičevka contained relatively high concentration of the most deficient EAAs (threonine, valine, leucine, lysine,). The highest value (29.31 g 100 g-l protein) of total essential amino acids (TEAA), 59.79 g 100 g-l protein of total nonessential amino acids (TNEAA) as well as the highest value (90.35 g 100 g-l protein) of total amino acids (TAA). Obtained results provide variability of wheat variety on the base of amino acid contents and indicate reliable variety for selecting desirable parents in breeding program for improving nutrient quality © 2023, Genetika.All Rights Reserved.",
journal = "Genetika, Genetika",
title = "PROTEIN CONTENT AND AMINO ACID COMPOSITION IN SEED OF BREAD WHEAT (Triticum aestivum L.)",
pages = "318-301",
number = "1",
volume = "55",
doi = "10.2298/GENSR23010301U"
}

Urošević, D., Knežević, D., Branković, G., Novoselskaya-Dragovich, A. Yu., Kudryavtsev, A. M., Stojšin, M. M., Mićanović, D.,& Zečević, V.. (2023). PROTEIN CONTENT AND AMINO ACID COMPOSITION IN SEED OF BREAD WHEAT (Triticum aestivum L.). in Genetika, 55(1), 301-318.
https://doi.org/10.2298/GENSR23010301U

Urošević D, Knežević D, Branković G, Novoselskaya-Dragovich AY, Kudryavtsev AM, Stojšin MM, Mićanović D, Zečević V. PROTEIN CONTENT AND AMINO ACID COMPOSITION IN SEED OF BREAD WHEAT (Triticum aestivum L.). in Genetika. 2023;55(1):301-318.
doi:10.2298/GENSR23010301U .

Urošević, Dušan, Knežević, Desimir, Branković, Gordana, Novoselskaya-Dragovich, Aleksandra Yu., Kudryavtsev, Alexander M., Stojšin, Mirela M., Mićanović, Danica, Zečević, Veselinka, "PROTEIN CONTENT AND AMINO ACID COMPOSITION IN SEED OF BREAD WHEAT (Triticum aestivum L.)" in Genetika, 55, no. 1 (2023):301-318,
https://doi.org/10.2298/GENSR23010301U . .

TY  - JOUR
AU  - Luković, K.
AU  - Prodanović, Slaven
AU  - Perisić, V
AU  - Milovanović, M.
AU  - Rajicić, V
AU  - Zečević, Veselinka
PY  - 2020
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5319
AB  - In these studies, 14 winter wheat (Triticum aestivum ssp vulgare L.) genotypes were analyzed in three localities across the Republic of Serbia. Some morphological and the most important productive traits were analysed in order to determine the relationship between grain yield and these traits in the year with extreme wet conditions during period of intensive wheat development (April-June period). According to our results, period of stem extension, heading and grain filling characterized by lower air temperatures and extremely high rainfall at all three localities, had detrimental effects on the grain filling process. The genotypes KG-191/5-13 and KG-1/6 achieved above average values of the most desirable traits in all three localities, and can be singled out as desirable parents in breeding programs to create new and improved varieties of wheat. Based on the principal component analysis and cluster analysis, two groups of similar genotypes were distinguished. The highest degree of positive correlation was found between diameter of the first internode and diameter of top internode; number of spikelets per spike and number of fertile spikelets per spike; grain mass/spike and grain mass/plant; plant height and length of the top internode.
PB  - Corvinus Univ Budapest, Budapest
T2  - Applied Ecology and Environmental Research
T1  - Multivariate analysis of morphological traits and the most important productive traits of wheat in extreme wet conditions
EP  - 5871
IS  - 4
SP  - 5857
VL  - 18
DO  - 10.15666/aeer/1804_58575871
ER  - 

@article{
author = "Luković, K. and Prodanović, Slaven and Perisić, V and Milovanović, M. and Rajicić, V and Zečević, Veselinka",
year = "2020",
abstract = "In these studies, 14 winter wheat (Triticum aestivum ssp vulgare L.) genotypes were analyzed in three localities across the Republic of Serbia. Some morphological and the most important productive traits were analysed in order to determine the relationship between grain yield and these traits in the year with extreme wet conditions during period of intensive wheat development (April-June period). According to our results, period of stem extension, heading and grain filling characterized by lower air temperatures and extremely high rainfall at all three localities, had detrimental effects on the grain filling process. The genotypes KG-191/5-13 and KG-1/6 achieved above average values of the most desirable traits in all three localities, and can be singled out as desirable parents in breeding programs to create new and improved varieties of wheat. Based on the principal component analysis and cluster analysis, two groups of similar genotypes were distinguished. The highest degree of positive correlation was found between diameter of the first internode and diameter of top internode; number of spikelets per spike and number of fertile spikelets per spike; grain mass/spike and grain mass/plant; plant height and length of the top internode.",
publisher = "Corvinus Univ Budapest, Budapest",
journal = "Applied Ecology and Environmental Research",
title = "Multivariate analysis of morphological traits and the most important productive traits of wheat in extreme wet conditions",
pages = "5871-5857",
number = "4",
volume = "18",
doi = "10.15666/aeer/1804_58575871"
}

Luković, K., Prodanović, S., Perisić, V., Milovanović, M., Rajicić, V.,& Zečević, V.. (2020). Multivariate analysis of morphological traits and the most important productive traits of wheat in extreme wet conditions. in Applied Ecology and Environmental Research
Corvinus Univ Budapest, Budapest., 18(4), 5857-5871.
https://doi.org/10.15666/aeer/1804_58575871

Luković K, Prodanović S, Perisić V, Milovanović M, Rajicić V, Zečević V. Multivariate analysis of morphological traits and the most important productive traits of wheat in extreme wet conditions. in Applied Ecology and Environmental Research. 2020;18(4):5857-5871.
doi:10.15666/aeer/1804_58575871 .

Luković, K., Prodanović, Slaven, Perisić, V, Milovanović, M., Rajicić, V, Zečević, Veselinka, "Multivariate analysis of morphological traits and the most important productive traits of wheat in extreme wet conditions" in Applied Ecology and Environmental Research, 18, no. 4 (2020):5857-5871,
https://doi.org/10.15666/aeer/1804_58575871 . .

TY  - JOUR
AU  - Djurić, Nenad
AU  - Prodanović, Slaven
AU  - Branković, Gordana
AU  - Djekić, Vera
AU  - Cvijanović, Gorica
AU  - Žilić, Sladjana
AU  - Dragičević, Vesna
AU  - Zečević, Veselinka
AU  - Dozet, Gordana
PY  - 2018
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4732
AB  - The investigation of three wheat varieties (PKB Talas, BG Merkur and PKB Lepoklasa) carried out at experimental field and laboratory of Institute PKB Agroekonomik, during two years 2009 and 2010. Correlations between morphological and production traits of plants number of shoots, number of spikelets per spike, number of grains per spike, 1000 grain weight and grain weight per spike, were studied. Correlations were observed separately for three Institute PKB Agroekonomik varieties. The manner of preparing data for calculating correlations influences obtained correlation values, and these values can differ substantially. Correlations calculated based on aggregated data are higher than those based on all data. Correlations differ for different varieties, which is logical, because each variety has a different genotype and specific genes forming various interactions. Taking into account all three varieties, high and positive correlations were found between: number of grains per spike and grain weight per spike (>0.78), number of spikelets per spike and number of grains per spike (>0.79), and number of spikelets per spike and grain weight per spike (>0.73).
PB  - Ars Docendi, Bucharest
T2  - Romanian Biotechnological Letters
T1  - Correlation-Regression Analysis of Morphological-Production Traits of Wheat Varieties
EP  - 13465
IS  - 2
SP  - 13457
VL  - 23
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_4732
ER  - 

@article{
author = "Djurić, Nenad and Prodanović, Slaven and Branković, Gordana and Djekić, Vera and Cvijanović, Gorica and Žilić, Sladjana and Dragičević, Vesna and Zečević, Veselinka and Dozet, Gordana",
year = "2018",
abstract = "The investigation of three wheat varieties (PKB Talas, BG Merkur and PKB Lepoklasa) carried out at experimental field and laboratory of Institute PKB Agroekonomik, during two years 2009 and 2010. Correlations between morphological and production traits of plants number of shoots, number of spikelets per spike, number of grains per spike, 1000 grain weight and grain weight per spike, were studied. Correlations were observed separately for three Institute PKB Agroekonomik varieties. The manner of preparing data for calculating correlations influences obtained correlation values, and these values can differ substantially. Correlations calculated based on aggregated data are higher than those based on all data. Correlations differ for different varieties, which is logical, because each variety has a different genotype and specific genes forming various interactions. Taking into account all three varieties, high and positive correlations were found between: number of grains per spike and grain weight per spike (>0.78), number of spikelets per spike and number of grains per spike (>0.79), and number of spikelets per spike and grain weight per spike (>0.73).",
publisher = "Ars Docendi, Bucharest",
journal = "Romanian Biotechnological Letters",
title = "Correlation-Regression Analysis of Morphological-Production Traits of Wheat Varieties",
pages = "13465-13457",
number = "2",
volume = "23",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_4732"
}

Djurić, N., Prodanović, S., Branković, G., Djekić, V., Cvijanović, G., Žilić, S., Dragičević, V., Zečević, V.,& Dozet, G.. (2018). Correlation-Regression Analysis of Morphological-Production Traits of Wheat Varieties. in Romanian Biotechnological Letters
Ars Docendi, Bucharest., 23(2), 13457-13465.
https://hdl.handle.net/21.15107/rcub_agrospace_4732

Djurić N, Prodanović S, Branković G, Djekić V, Cvijanović G, Žilić S, Dragičević V, Zečević V, Dozet G. Correlation-Regression Analysis of Morphological-Production Traits of Wheat Varieties. in Romanian Biotechnological Letters. 2018;23(2):13457-13465.
https://hdl.handle.net/21.15107/rcub_agrospace_4732 .

Djurić, Nenad, Prodanović, Slaven, Branković, Gordana, Djekić, Vera, Cvijanović, Gorica, Žilić, Sladjana, Dragičević, Vesna, Zečević, Veselinka, Dozet, Gordana, "Correlation-Regression Analysis of Morphological-Production Traits of Wheat Varieties" in Romanian Biotechnological Letters, 23, no. 2 (2018):13457-13465,
https://hdl.handle.net/21.15107/rcub_agrospace_4732 .

TY  - CONF
AU  - Knezević, Desimir
AU  - Zečević, Veselinka
AU  - Paunović, Aleksandar
AU  - Broćić, Zoran
AU  - Madić, Milomirka
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/5565
AB  - Creation of transgenic organisms should be endeavored necessary amount of food for the elimination of mankind hunger, increase yield and quality, resistance to diseases and pests, tolerance to high and low temperature properties and improvement of transportation and storage. Transgenic organisms are characterized by a higher content of protein, oil, and starch specific structure. Their use in the diet can have different effects on human health from uncontrolled use. Due to the relatively short period since the creation of transgenic organisms and for greater security man and the ecosystem of their production and trade is within the legal regulations and legislation.
AB  - Čovek je tokom svog istorijskog razvoja do danas bio, između ostalog, kreativno biće u interakciji sa spoljašnjom sredinom. Birao je najbolje vrste, najbolje plodove, najbolja staništa i u tom ambijentu je proizvodio i kreirao bolje od postojećeg pa i u živom svetu. Transgeni oganizmi su kreirani u cilju povećanja prinosa, kvaliteta i adaptivnosti a time i obezbeđenje hrane za ljudsku populaciju koja se brojno umnožava. Za neke transgene organizme je ustanovljeno negativno delovanje na druge organizme i ekosistem. Tako su se u oblasti biotehnologije postigli vrhunski rezultati koji ne ostavljaju ravnodušnim pojedince, grupe i udruženja u naučnoj, stručnoj i široj javnosti kod nas i u svetu.
C3  - 19. Savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 07.- 08. 03. 2014.
T1  - Transgenic Organism Biotechnology Power
T1  - Transgeni organizmi-biotehnološka moć
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_5565
ER  - 

@conference{
author = "Knezević, Desimir and Zečević, Veselinka and Paunović, Aleksandar and Broćić, Zoran and Madić, Milomirka",
year = "2014",
abstract = "Creation of transgenic organisms should be endeavored necessary amount of food for the elimination of mankind hunger, increase yield and quality, resistance to diseases and pests, tolerance to high and low temperature properties and improvement of transportation and storage. Transgenic organisms are characterized by a higher content of protein, oil, and starch specific structure. Their use in the diet can have different effects on human health from uncontrolled use. Due to the relatively short period since the creation of transgenic organisms and for greater security man and the ecosystem of their production and trade is within the legal regulations and legislation., Čovek je tokom svog istorijskog razvoja do danas bio, između ostalog, kreativno biće u interakciji sa spoljašnjom sredinom. Birao je najbolje vrste, najbolje plodove, najbolja staništa i u tom ambijentu je proizvodio i kreirao bolje od postojećeg pa i u živom svetu. Transgeni oganizmi su kreirani u cilju povećanja prinosa, kvaliteta i adaptivnosti a time i obezbeđenje hrane za ljudsku populaciju koja se brojno umnožava. Za neke transgene organizme je ustanovljeno negativno delovanje na druge organizme i ekosistem. Tako su se u oblasti biotehnologije postigli vrhunski rezultati koji ne ostavljaju ravnodušnim pojedince, grupe i udruženja u naučnoj, stručnoj i široj javnosti kod nas i u svetu.",
journal = "19. Savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 07.- 08. 03. 2014.",
title = "Transgenic Organism Biotechnology Power, Transgeni organizmi-biotehnološka moć",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_5565"
}

Knezević, D., Zečević, V., Paunović, A., Broćić, Z.,& Madić, M.. (2014). Transgenic Organism Biotechnology Power. in 19. Savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 07.- 08. 03. 2014..
https://hdl.handle.net/21.15107/rcub_agrospace_5565

Knezević D, Zečević V, Paunović A, Broćić Z, Madić M. Transgenic Organism Biotechnology Power. in 19. Savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 07.- 08. 03. 2014.. 2014;.
https://hdl.handle.net/21.15107/rcub_agrospace_5565 .

Knezević, Desimir, Zečević, Veselinka, Paunović, Aleksandar, Broćić, Zoran, Madić, Milomirka, "Transgenic Organism Biotechnology Power" in 19. Savetovanje o biotehnologiji sa međunarodnim učešćem, Čačak, 07.- 08. 03. 2014. (2014),
https://hdl.handle.net/21.15107/rcub_agrospace_5565 .

TY  - JOUR
AU  - Mićanović, Danica
AU  - Hirsch, Penny
AU  - Curnow, Phil
AU  - Zečević, Veselinka
AU  - Raičević, Vera
AU  - Knežević, Desimir
PY  - 2005
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1109
AB  - The ability to colonize wheat by determinate strain in soil, in competition with the total soil microflora, was investigated with three mutants of strain pseudomonads which contains the nptII gene, which codes for an aminoglycosidephosphotransferase, this enzyme confers antibiotic resistance to both neomycin and kanamycin. Number of bacteria was obtained from bulk soil, rhizosphere and rhizoplane, using phase contrast microscopy. Mutants were detected using selective agar for pseudomonas supplied with neomycin and genomic fingerprinting of bacteria used molecular genetic method ERIC-PCR. On the rhizoplane, number of all three mutants where similar at about 104 per 3cm root, but the total heterotroph population varied so that the proportion of mutants appeared to vary from 6.3% for PCM40074 to 20% for PCM40313, to 84% for PCM40326. This could be due occasion but by observation of 24 colonies of heterotrophs isolated on TSA, 10 were positively identified as PCM40326 using ERIC-PCR and antibiotic- resistance phenotype on selective agar and indicates that it may be more competitive than the other two mutants.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Savremena poljoprivreda
T1  - Wheat colonization by determinate bacteria
EP  - 317
IS  - 3-4
SP  - 312
VL  - 54
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1109
ER  - 

@article{
author = "Mićanović, Danica and Hirsch, Penny and Curnow, Phil and Zečević, Veselinka and Raičević, Vera and Knežević, Desimir",
year = "2005",
abstract = "The ability to colonize wheat by determinate strain in soil, in competition with the total soil microflora, was investigated with three mutants of strain pseudomonads which contains the nptII gene, which codes for an aminoglycosidephosphotransferase, this enzyme confers antibiotic resistance to both neomycin and kanamycin. Number of bacteria was obtained from bulk soil, rhizosphere and rhizoplane, using phase contrast microscopy. Mutants were detected using selective agar for pseudomonas supplied with neomycin and genomic fingerprinting of bacteria used molecular genetic method ERIC-PCR. On the rhizoplane, number of all three mutants where similar at about 104 per 3cm root, but the total heterotroph population varied so that the proportion of mutants appeared to vary from 6.3% for PCM40074 to 20% for PCM40313, to 84% for PCM40326. This could be due occasion but by observation of 24 colonies of heterotrophs isolated on TSA, 10 were positively identified as PCM40326 using ERIC-PCR and antibiotic- resistance phenotype on selective agar and indicates that it may be more competitive than the other two mutants.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Savremena poljoprivreda",
title = "Wheat colonization by determinate bacteria",
pages = "317-312",
number = "3-4",
volume = "54",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1109"
}

Mićanović, D., Hirsch, P., Curnow, P., Zečević, V., Raičević, V.,& Knežević, D.. (2005). Wheat colonization by determinate bacteria. in Savremena poljoprivreda
Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad., 54(3-4), 312-317.
https://hdl.handle.net/21.15107/rcub_agrospace_1109

Mićanović D, Hirsch P, Curnow P, Zečević V, Raičević V, Knežević D. Wheat colonization by determinate bacteria. in Savremena poljoprivreda. 2005;54(3-4):312-317.
https://hdl.handle.net/21.15107/rcub_agrospace_1109 .

Mićanović, Danica, Hirsch, Penny, Curnow, Phil, Zečević, Veselinka, Raičević, Vera, Knežević, Desimir, "Wheat colonization by determinate bacteria" in Savremena poljoprivreda, 54, no. 3-4 (2005):312-317,
https://hdl.handle.net/21.15107/rcub_agrospace_1109 .