TY  - JOUR
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Sunjog, Karolina
AU  - Subotić, Srđan
AU  - Višnjić-Jeftić, Željka
AU  - Rašković, Božidar
AU  - Poleksić, Vesna
AU  - Vuković-Gačić, Branka
AU  - Lenhardt, Mirjana
PY  - 2023
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/6354
AB  - In this study a battery of bioassays, both in vivo (metals and metalloids concentrations, erythrocyte morphometry, comet assay, micronucleus assay, and histopathological analyses) on vimba bream Vimba vimba (L., 1758) and white bream Blicca bjoerkna (L., 1758), and in vitro (treatment of HepG2 cells with native water samples) was applied to assess the harmful potential of untreated wastewater. Faecal indicator bacteria were quantified to assess the microbiological water quality. Vimba bream had significantly higher Fe concentrations in both liver and muscle, while white bream had higher Ca and Cu concentrations in liver. Vimba bream had a significantly higher level of DNA damage in both liver and blood cells, in comparison to white bream. Low levels of micronucleus and nuclear abnormalities were observed in both species. Erythrocytes morphometry did not show significant interspecific differences. Histopathological analyses revealed a similar response of the studied species, with a significantly higher presence of ceroid pigments in the liver of vimba bream. Treatment of HepG2 cells revealed the high genotoxic potential of water downstream of the discharge point. The results of this study clearly demonstrate the importance of effect-based monitoring, in order to enforce more efficient management of natural resources and implementation of wastewater treatment systems. © 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
T2  - Ecotoxicology
T2  - Ecotoxicology
T1  - Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia
DO  - 10.1007/s10646-023-02663-6
ER  - 

@article{
author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Sunjog, Karolina and Subotić, Srđan and Višnjić-Jeftić, Željka and Rašković, Božidar and Poleksić, Vesna and Vuković-Gačić, Branka and Lenhardt, Mirjana",
year = "2023",
abstract = "In this study a battery of bioassays, both in vivo (metals and metalloids concentrations, erythrocyte morphometry, comet assay, micronucleus assay, and histopathological analyses) on vimba bream Vimba vimba (L., 1758) and white bream Blicca bjoerkna (L., 1758), and in vitro (treatment of HepG2 cells with native water samples) was applied to assess the harmful potential of untreated wastewater. Faecal indicator bacteria were quantified to assess the microbiological water quality. Vimba bream had significantly higher Fe concentrations in both liver and muscle, while white bream had higher Ca and Cu concentrations in liver. Vimba bream had a significantly higher level of DNA damage in both liver and blood cells, in comparison to white bream. Low levels of micronucleus and nuclear abnormalities were observed in both species. Erythrocytes morphometry did not show significant interspecific differences. Histopathological analyses revealed a similar response of the studied species, with a significantly higher presence of ceroid pigments in the liver of vimba bream. Treatment of HepG2 cells revealed the high genotoxic potential of water downstream of the discharge point. The results of this study clearly demonstrate the importance of effect-based monitoring, in order to enforce more efficient management of natural resources and implementation of wastewater treatment systems. © 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.",
journal = "Ecotoxicology, Ecotoxicology",
title = "Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia",
doi = "10.1007/s10646-023-02663-6"
}

Kostić-Vuković, J., Kolarević, S., Sunjog, K., Subotić, S., Višnjić-Jeftić, Ž., Rašković, B., Poleksić, V., Vuković-Gačić, B.,& Lenhardt, M.. (2023). Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia. in Ecotoxicology.
https://doi.org/10.1007/s10646-023-02663-6

Kostić-Vuković J, Kolarević S, Sunjog K, Subotić S, Višnjić-Jeftić Ž, Rašković B, Poleksić V, Vuković-Gačić B, Lenhardt M. Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia. in Ecotoxicology. 2023;.
doi:10.1007/s10646-023-02663-6 .

Kostić-Vuković, Jovana, Kolarević, Stoimir, Sunjog, Karolina, Subotić, Srđan, Višnjić-Jeftić, Željka, Rašković, Božidar, Poleksić, Vesna, Vuković-Gačić, Branka, Lenhardt, Mirjana, "Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia" in Ecotoxicology (2023),
https://doi.org/10.1007/s10646-023-02663-6 . .

TY  - JOUR
AU  - Lenhardt, Mirjana
AU  - Poleksić, Vesna
AU  - Vuković-Gačić, Branka
AU  - Rašković, Božidar
AU  - Sunjog, Karolina
AU  - Kolarević, Stoimir
AU  - Jarić, Ivan
AU  - Gačić, Zoran
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3781
AB  - Freshwater bodies receive high levels of different toxicants. Since fish are typically situated at the top of aquatic food chains, they have a good potential to be used as indicators of water pollution. Assessment of the presence of pollutants and their toxicity can be efficiently performed by the combined use of analytical chemistry, bioassays and applied mathematics. In this study, we present the general approach of the integrated use of different fish related parameters to assess the status and pollution levels of waterbodies. We discuss our previous experiences within the field of analytical toxicology, toxicological pathology and toxicity testing, as well as biomathematical and statistical methods that are able to provide for integration of results acquired by each of the specific methods. We discuss advantages and shortcomings of each of the methods, and present necessary future steps in the method development. Since the industrial and domestic wastewaters in Serbia are still not processed before being released into watercourses, they pose a serious risk for aquatic ecosystems and public health. Consequently, described pollution indicators and genotoxicity parameters represent an essential tool for efficient monitoring of aquatic ecosystems. Methodological approach presented here might be of interest for scientists and managers dealing with the ecotoxicological research and monitoring of freshwater ecosystems.
PB  - University of Ljubljana - Veterinary Faculty
T2  - Slovenian Veterinary Research
T1  - Integrated use of different fish related parameters to assess the status of water bodies
EP  - 13
IS  - 1
SP  - 5
VL  - 52
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_3781
ER  - 

@article{
author = "Lenhardt, Mirjana and Poleksić, Vesna and Vuković-Gačić, Branka and Rašković, Božidar and Sunjog, Karolina and Kolarević, Stoimir and Jarić, Ivan and Gačić, Zoran",
year = "2015",
abstract = "Freshwater bodies receive high levels of different toxicants. Since fish are typically situated at the top of aquatic food chains, they have a good potential to be used as indicators of water pollution. Assessment of the presence of pollutants and their toxicity can be efficiently performed by the combined use of analytical chemistry, bioassays and applied mathematics. In this study, we present the general approach of the integrated use of different fish related parameters to assess the status and pollution levels of waterbodies. We discuss our previous experiences within the field of analytical toxicology, toxicological pathology and toxicity testing, as well as biomathematical and statistical methods that are able to provide for integration of results acquired by each of the specific methods. We discuss advantages and shortcomings of each of the methods, and present necessary future steps in the method development. Since the industrial and domestic wastewaters in Serbia are still not processed before being released into watercourses, they pose a serious risk for aquatic ecosystems and public health. Consequently, described pollution indicators and genotoxicity parameters represent an essential tool for efficient monitoring of aquatic ecosystems. Methodological approach presented here might be of interest for scientists and managers dealing with the ecotoxicological research and monitoring of freshwater ecosystems.",
publisher = "University of Ljubljana - Veterinary Faculty",
journal = "Slovenian Veterinary Research",
title = "Integrated use of different fish related parameters to assess the status of water bodies",
pages = "13-5",
number = "1",
volume = "52",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_3781"
}

Lenhardt, M., Poleksić, V., Vuković-Gačić, B., Rašković, B., Sunjog, K., Kolarević, S., Jarić, I.,& Gačić, Z.. (2015). Integrated use of different fish related parameters to assess the status of water bodies. in Slovenian Veterinary Research
University of Ljubljana - Veterinary Faculty., 52(1), 5-13.
https://hdl.handle.net/21.15107/rcub_agrospace_3781

Lenhardt M, Poleksić V, Vuković-Gačić B, Rašković B, Sunjog K, Kolarević S, Jarić I, Gačić Z. Integrated use of different fish related parameters to assess the status of water bodies. in Slovenian Veterinary Research. 2015;52(1):5-13.
https://hdl.handle.net/21.15107/rcub_agrospace_3781 .

Lenhardt, Mirjana, Poleksić, Vesna, Vuković-Gačić, Branka, Rašković, Božidar, Sunjog, Karolina, Kolarević, Stoimir, Jarić, Ivan, Gačić, Zoran, "Integrated use of different fish related parameters to assess the status of water bodies" in Slovenian Veterinary Research, 52, no. 1 (2015):5-13,
https://hdl.handle.net/21.15107/rcub_agrospace_3781 .