TY  - JOUR
AU  - Vučetić, Andja
AU  - Petrović-Obradović, Olivera
AU  - Margaritopoulos, John
AU  - Skouras, Panagiotis
PY  - 2007
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/1382
AB  - Peach/potato aphid Myzus persicae (Sulzer) has developed three mechanisms of resistance: overproduction of one or two closely related carboxylesterases (E4 and FE4), production of modified acetylcholinesterase (MACE and knockdown resistance). Biochemical diagnostic methods can be used to identify levels of carboxylesterase production and sensitivity of modified acetylcholinesterase and, based on the values acquired we can classify aphids into one of the resistance categories. We used two tests: the total esterase activity test and test for checking sensitivity of acetylcholinesterase (MACE). Myzus periscae persicae from peach and Myzus persicae nicotianae from tobacco were tested. The total esterase activity test showed that 65% of the aphids tested were moderately resistant and 35% were highly resistant. Resistance based on the production of modified acetylcholinesterase is less represented because 80% of the aphids tested were susceptible to pirimicarb, which was used in the experiment. Also, both tests showed that aphids from tobacco were more susceptible than aphids from peach.
AB  - Kod Myzus persicae (Sulzer) do sada su utvrđena tri tipa rezistentosti: povećana produkcija karboksilesteraza E4 i FE4, stvaranje modifikovane acetilholinesteraze i "knock-down" rezistentnost. Biohemijskim metodama moguće je izmeriti količinu karboksilesteraza, kao i aktivnosti modifikovane acetilholinesteraze i na osnovu dobijenih vrednosti, vaši svrstati u neku od kategorija rezistentnosti. Korišćena su dva testa, test totalne esterazne aktivnosti i test za proveru aktivnosti modifikovane acetilholinesteraze (MACE). Testirane su Myzus persicae persicae sa breskve i Myzus persicae nicotianae sa duvana. Test totalne esterazne aktivnosti pokazao je da 65% testiranih jedinki pripada kategoriji umereno rezistentnih vašiju, dok je 35% veoma rezistentno. Rezistentnost koja se zasniva na stvaranju modifikovane acetilholinesteraze manje je zastupljena, jer je čak 80% testiranih vašiju osetljivo na dejstvo pirimikarba, koji je korišćen u testu. Takođe, oba testa su pokazala da su vaši sa duvana osetljivije na dejstvo insekticida od vašiju sa breskve.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Determination of resistance levels of Myzus persicae (Sulzer) by biochemical methods
T1  - Utvđivanje nivoa rezistentnosti Myzus persicae (Sulzer) biohemijskim metodama
EP  - 299
IS  - 4
SP  - 291
VL  - 22
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_1382
ER  - 

@article{
author = "Vučetić, Andja and Petrović-Obradović, Olivera and Margaritopoulos, John and Skouras, Panagiotis",
year = "2007",
abstract = "Peach/potato aphid Myzus persicae (Sulzer) has developed three mechanisms of resistance: overproduction of one or two closely related carboxylesterases (E4 and FE4), production of modified acetylcholinesterase (MACE and knockdown resistance). Biochemical diagnostic methods can be used to identify levels of carboxylesterase production and sensitivity of modified acetylcholinesterase and, based on the values acquired we can classify aphids into one of the resistance categories. We used two tests: the total esterase activity test and test for checking sensitivity of acetylcholinesterase (MACE). Myzus periscae persicae from peach and Myzus persicae nicotianae from tobacco were tested. The total esterase activity test showed that 65% of the aphids tested were moderately resistant and 35% were highly resistant. Resistance based on the production of modified acetylcholinesterase is less represented because 80% of the aphids tested were susceptible to pirimicarb, which was used in the experiment. Also, both tests showed that aphids from tobacco were more susceptible than aphids from peach., Kod Myzus persicae (Sulzer) do sada su utvrđena tri tipa rezistentosti: povećana produkcija karboksilesteraza E4 i FE4, stvaranje modifikovane acetilholinesteraze i "knock-down" rezistentnost. Biohemijskim metodama moguće je izmeriti količinu karboksilesteraza, kao i aktivnosti modifikovane acetilholinesteraze i na osnovu dobijenih vrednosti, vaši svrstati u neku od kategorija rezistentnosti. Korišćena su dva testa, test totalne esterazne aktivnosti i test za proveru aktivnosti modifikovane acetilholinesteraze (MACE). Testirane su Myzus persicae persicae sa breskve i Myzus persicae nicotianae sa duvana. Test totalne esterazne aktivnosti pokazao je da 65% testiranih jedinki pripada kategoriji umereno rezistentnih vašiju, dok je 35% veoma rezistentno. Rezistentnost koja se zasniva na stvaranju modifikovane acetilholinesteraze manje je zastupljena, jer je čak 80% testiranih vašiju osetljivo na dejstvo pirimikarba, koji je korišćen u testu. Takođe, oba testa su pokazala da su vaši sa duvana osetljivije na dejstvo insekticida od vašiju sa breskve.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Determination of resistance levels of Myzus persicae (Sulzer) by biochemical methods, Utvđivanje nivoa rezistentnosti Myzus persicae (Sulzer) biohemijskim metodama",
pages = "299-291",
number = "4",
volume = "22",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_1382"
}

Vučetić, A., Petrović-Obradović, O., Margaritopoulos, J.,& Skouras, P.. (2007). Determination of resistance levels of Myzus persicae (Sulzer) by biochemical methods. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 22(4), 291-299.
https://hdl.handle.net/21.15107/rcub_agrospace_1382

Vučetić A, Petrović-Obradović O, Margaritopoulos J, Skouras P. Determination of resistance levels of Myzus persicae (Sulzer) by biochemical methods. in Pesticidi i fitomedicina. 2007;22(4):291-299.
https://hdl.handle.net/21.15107/rcub_agrospace_1382 .

Vučetić, Andja, Petrović-Obradović, Olivera, Margaritopoulos, John, Skouras, Panagiotis, "Determination of resistance levels of Myzus persicae (Sulzer) by biochemical methods" in Pesticidi i fitomedicina, 22, no. 4 (2007):291-299,
https://hdl.handle.net/21.15107/rcub_agrospace_1382 .