TY  - JOUR
AU  - Kostić, Jovana
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarević, Margareta
AU  - Aborgiba, Mustafa
AU  - Gačić, Zoran
AU  - Paunović, Momir
AU  - Visnjić-Jeftić, Zeljka
AU  - Rašković, Božidar
AU  - Poleksić, Vesna
AU  - Lenhardt, Mirjana
AU  - Vuković-Gačić, Branka
PY  - 2017
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/4465
AB  - Biomarkers attract increasing attention in environmental studies, as a tool for detection of exposure and effects of pollution, from both natural and anthropogenic sources. This study aims to assess the impact of multiple stressors during distinctive seasons, covering also extreme hydrological events (extensive flooding in the mid May 2014), on different levels of biological organization in the liver and gills of three closely related freshwater breams. Our previous study on DNA damage in blood cells of these specimens showed increased DNA damage in June 2014, one month after the flooding event. As a continuation of that research, the present study was conducted. As a biomarker of exposure DNA damage was measured by applying the alkaline comet assay, while histopathological alterations were monitored as a biomarker of effect. Additionally, concentrations of metals and metalloids in gills, liver and muscle were assessed. Sampling of fish tissues was performed in 2014, during winter (January and February), spring (March and early June) and summer (late June, July and August). Significant seasonal difference in DNA damage was observed for both tissues. During spring and summer the level of DNA damage in gills was significantly higher when compared to the liver. Histopathological analyses showed higher frequency of alterations in gills during spring, and in liver during summer, but without a significant seasonal difference. Gills had the highest concentration of metals and metalloids during the spring and summer, and liver duringwinter. Muscle was the least affected tissue during all three seasons. This study highlighted the importance of the multiple biomarker approach and the use of different fish tissues in assessment of surface water pollution.
PB  - Elsevier Science Bv, Amsterdam
T2  - Science of the Total Environment
T1  - The impact of multiple stressors on the biomarkers response in gills and liver of freshwater breams during different seasons
EP  - 1681
SP  - 1670
VL  - 601
DO  - 10.1016/j.scitotenv.2017.05.273
ER  - 

@article{
author = "Kostić, Jovana and Kolarević, Stoimir and Kracun-Kolarević, Margareta and Aborgiba, Mustafa and Gačić, Zoran and Paunović, Momir and Visnjić-Jeftić, Zeljka and Rašković, Božidar and Poleksić, Vesna and Lenhardt, Mirjana and Vuković-Gačić, Branka",
year = "2017",
abstract = "Biomarkers attract increasing attention in environmental studies, as a tool for detection of exposure and effects of pollution, from both natural and anthropogenic sources. This study aims to assess the impact of multiple stressors during distinctive seasons, covering also extreme hydrological events (extensive flooding in the mid May 2014), on different levels of biological organization in the liver and gills of three closely related freshwater breams. Our previous study on DNA damage in blood cells of these specimens showed increased DNA damage in June 2014, one month after the flooding event. As a continuation of that research, the present study was conducted. As a biomarker of exposure DNA damage was measured by applying the alkaline comet assay, while histopathological alterations were monitored as a biomarker of effect. Additionally, concentrations of metals and metalloids in gills, liver and muscle were assessed. Sampling of fish tissues was performed in 2014, during winter (January and February), spring (March and early June) and summer (late June, July and August). Significant seasonal difference in DNA damage was observed for both tissues. During spring and summer the level of DNA damage in gills was significantly higher when compared to the liver. Histopathological analyses showed higher frequency of alterations in gills during spring, and in liver during summer, but without a significant seasonal difference. Gills had the highest concentration of metals and metalloids during the spring and summer, and liver duringwinter. Muscle was the least affected tissue during all three seasons. This study highlighted the importance of the multiple biomarker approach and the use of different fish tissues in assessment of surface water pollution.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Science of the Total Environment",
title = "The impact of multiple stressors on the biomarkers response in gills and liver of freshwater breams during different seasons",
pages = "1681-1670",
volume = "601",
doi = "10.1016/j.scitotenv.2017.05.273"
}

Kostić, J., Kolarević, S., Kracun-Kolarević, M., Aborgiba, M., Gačić, Z., Paunović, M., Visnjić-Jeftić, Z., Rašković, B., Poleksić, V., Lenhardt, M.,& Vuković-Gačić, B.. (2017). The impact of multiple stressors on the biomarkers response in gills and liver of freshwater breams during different seasons. in Science of the Total Environment
Elsevier Science Bv, Amsterdam., 601, 1670-1681.
https://doi.org/10.1016/j.scitotenv.2017.05.273

Kostić J, Kolarević S, Kracun-Kolarević M, Aborgiba M, Gačić Z, Paunović M, Visnjić-Jeftić Z, Rašković B, Poleksić V, Lenhardt M, Vuković-Gačić B. The impact of multiple stressors on the biomarkers response in gills and liver of freshwater breams during different seasons. in Science of the Total Environment. 2017;601:1670-1681.
doi:10.1016/j.scitotenv.2017.05.273 .

Kostić, Jovana, Kolarević, Stoimir, Kracun-Kolarević, Margareta, Aborgiba, Mustafa, Gačić, Zoran, Paunović, Momir, Visnjić-Jeftić, Zeljka, Rašković, Božidar, Poleksić, Vesna, Lenhardt, Mirjana, Vuković-Gačić, Branka, "The impact of multiple stressors on the biomarkers response in gills and liver of freshwater breams during different seasons" in Science of the Total Environment, 601 (2017):1670-1681,
https://doi.org/10.1016/j.scitotenv.2017.05.273 . .

TY  - JOUR
AU  - Rašković, Božidar
AU  - Poleksić, Vesna
AU  - Visnjić-Jeftić, Zeljka
AU  - Skorić, Stefan
AU  - Gačić, Zoran
AU  - Djikanović, Vesna
AU  - Jarić, Ivan
AU  - Lenhardt, Mirjana
PY  - 2015
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3690
AB  - The bottom feeding fish species have a good potential to be used for assessments of pollution, as they are under pressure from pollutants from both water and sediments. In this study, the level of similarity of histopathological responses to pollution in gills and liver between barbel (Barbus barbus) and sterlet (Acipenser ruthenus) from the Danube River was assessed, and compared with elemental concentrations in their gills, liver, and muscle. Results indicate that the detected metal concentrations were likely cause of different tissue responses in gills and liver of the two investigated fish species. Statistical analysis indicated a clear differentiation of the two species based on elemental concentrations and the level of histopathological changes in gills and liver. Metal concentrations exceeded maximum acceptable concentrations in a number of analyzed specimens, which indicates the importance of this type of monitoring. Results indicate that barbel is a better indicator for specific, rather narrow sites, whereas sterlet is a better indicator of larger (longer) water current segments. Obtained information could be of importance for both scientists and fishery and water management authorities working on the development of water monitoring programs.
PB  - Wiley, Hoboken
T2  - Environmental Toxicology
T1  - Use of histopathology and elemental accumulation in different organs of two benthophagous fish species as indicators of river pollution
EP  - 1161
IS  - 10
SP  - 1153
VL  - 30
DO  - 10.1002/tox.21988
ER  - 

@article{
author = "Rašković, Božidar and Poleksić, Vesna and Visnjić-Jeftić, Zeljka and Skorić, Stefan and Gačić, Zoran and Djikanović, Vesna and Jarić, Ivan and Lenhardt, Mirjana",
year = "2015",
abstract = "The bottom feeding fish species have a good potential to be used for assessments of pollution, as they are under pressure from pollutants from both water and sediments. In this study, the level of similarity of histopathological responses to pollution in gills and liver between barbel (Barbus barbus) and sterlet (Acipenser ruthenus) from the Danube River was assessed, and compared with elemental concentrations in their gills, liver, and muscle. Results indicate that the detected metal concentrations were likely cause of different tissue responses in gills and liver of the two investigated fish species. Statistical analysis indicated a clear differentiation of the two species based on elemental concentrations and the level of histopathological changes in gills and liver. Metal concentrations exceeded maximum acceptable concentrations in a number of analyzed specimens, which indicates the importance of this type of monitoring. Results indicate that barbel is a better indicator for specific, rather narrow sites, whereas sterlet is a better indicator of larger (longer) water current segments. Obtained information could be of importance for both scientists and fishery and water management authorities working on the development of water monitoring programs.",
publisher = "Wiley, Hoboken",
journal = "Environmental Toxicology",
title = "Use of histopathology and elemental accumulation in different organs of two benthophagous fish species as indicators of river pollution",
pages = "1161-1153",
number = "10",
volume = "30",
doi = "10.1002/tox.21988"
}

Rašković, B., Poleksić, V., Visnjić-Jeftić, Z., Skorić, S., Gačić, Z., Djikanović, V., Jarić, I.,& Lenhardt, M.. (2015). Use of histopathology and elemental accumulation in different organs of two benthophagous fish species as indicators of river pollution. in Environmental Toxicology
Wiley, Hoboken., 30(10), 1153-1161.
https://doi.org/10.1002/tox.21988

Rašković B, Poleksić V, Visnjić-Jeftić Z, Skorić S, Gačić Z, Djikanović V, Jarić I, Lenhardt M. Use of histopathology and elemental accumulation in different organs of two benthophagous fish species as indicators of river pollution. in Environmental Toxicology. 2015;30(10):1153-1161.
doi:10.1002/tox.21988 .

Rašković, Božidar, Poleksić, Vesna, Visnjić-Jeftić, Zeljka, Skorić, Stefan, Gačić, Zoran, Djikanović, Vesna, Jarić, Ivan, Lenhardt, Mirjana, "Use of histopathology and elemental accumulation in different organs of two benthophagous fish species as indicators of river pollution" in Environmental Toxicology, 30, no. 10 (2015):1153-1161,
https://doi.org/10.1002/tox.21988 . .