TY  - JOUR
AU  - Pihler, Ivan
AU  - Kiprijanovska, Hrisula
AU  - Plavsa, Nada
AU  - Krajinović, Milan
AU  - Uzunov, Aleksandar
AU  - Kryger, Per
AU  - Nedić, Nebojša
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3422
AB  - In 1893. Jovan Zivanovic notes that based on the color of the abdomen of bees, two varieties of bees are present in Vojvodina, one dark and the other with two yellow rings on abdomen. Up to the present time there are mixed opinions about the presence of one or more populations of bees in Vojvodina, especially about the presence of the yellow variety in Banat. The aim of this paper is to establish the existence of one or more populations of bees, using genetic analysis of bees. Evaluation of genetic connections, diversity within the population and structure of the bee population in Vojvodina, were calculated on the basis of allele variation of 25 microsatellite loci. A genetic typification of the following microsatellites was performed: A8, A14, A24, A29, A43, A79, A88, A113, Ac11, Ac88, Ac139, Ac306, Ap15, Ap68, Ap85, Ap90, Ap223, Ap224, Ap226, Ap249, Ap273, Ap274, Ap288, At168, At188. 92% or 23 loci proved to be polymorphic in samples of bees from Srem and Backa, and 88% or 22 loci proved to be polymorphic in samples of bees from Banat. Heterozygosity calculated for the whole population is not significantly different from the expected heterozygosity. It was found that the obtained genetic differences between bees of Srem and Backa, and Banat region are not sufficient for these two populations to be considered separate.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Population-genetical characteristics of the bee population of Vojvodina
EP  - 226
IS  - 1
SP  - 219
VL  - 46
DO  - 10.2298/GENSR1401219P
ER  - 

@article{
author = "Pihler, Ivan and Kiprijanovska, Hrisula and Plavsa, Nada and Krajinović, Milan and Uzunov, Aleksandar and Kryger, Per and Nedić, Nebojša",
year = "2014",
abstract = "In 1893. Jovan Zivanovic notes that based on the color of the abdomen of bees, two varieties of bees are present in Vojvodina, one dark and the other with two yellow rings on abdomen. Up to the present time there are mixed opinions about the presence of one or more populations of bees in Vojvodina, especially about the presence of the yellow variety in Banat. The aim of this paper is to establish the existence of one or more populations of bees, using genetic analysis of bees. Evaluation of genetic connections, diversity within the population and structure of the bee population in Vojvodina, were calculated on the basis of allele variation of 25 microsatellite loci. A genetic typification of the following microsatellites was performed: A8, A14, A24, A29, A43, A79, A88, A113, Ac11, Ac88, Ac139, Ac306, Ap15, Ap68, Ap85, Ap90, Ap223, Ap224, Ap226, Ap249, Ap273, Ap274, Ap288, At168, At188. 92% or 23 loci proved to be polymorphic in samples of bees from Srem and Backa, and 88% or 22 loci proved to be polymorphic in samples of bees from Banat. Heterozygosity calculated for the whole population is not significantly different from the expected heterozygosity. It was found that the obtained genetic differences between bees of Srem and Backa, and Banat region are not sufficient for these two populations to be considered separate.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Population-genetical characteristics of the bee population of Vojvodina",
pages = "226-219",
number = "1",
volume = "46",
doi = "10.2298/GENSR1401219P"
}

Pihler, I., Kiprijanovska, H., Plavsa, N., Krajinović, M., Uzunov, A., Kryger, P.,& Nedić, N.. (2014). Population-genetical characteristics of the bee population of Vojvodina. in Genetika
Društvo genetičara Srbije, Beograd., 46(1), 219-226.
https://doi.org/10.2298/GENSR1401219P

Pihler I, Kiprijanovska H, Plavsa N, Krajinović M, Uzunov A, Kryger P, Nedić N. Population-genetical characteristics of the bee population of Vojvodina. in Genetika. 2014;46(1):219-226.
doi:10.2298/GENSR1401219P .

Pihler, Ivan, Kiprijanovska, Hrisula, Plavsa, Nada, Krajinović, Milan, Uzunov, Aleksandar, Kryger, Per, Nedić, Nebojša, "Population-genetical characteristics of the bee population of Vojvodina" in Genetika, 46, no. 1 (2014):219-226,
https://doi.org/10.2298/GENSR1401219P . .

TY  - JOUR
AU  - Nedić, Nebojša
AU  - Francis, Roy Mathew
AU  - Stanisavljević, Ljubiša
AU  - Pihler, Ivan
AU  - Kezić, Nikola
AU  - Bendixen, Christian
AU  - Kryger, Per
PY  - 2014
UR  - http://aspace.agrif.bg.ac.rs/handle/123456789/3415
AB  - Honey bee workers were sampled across the Serbian territory during 2009-2010 from mostly non-migratory apiaries to determine the population structure of these bees using morphometric, genetic, and spatial information. A total of 134 bees were sampled, of which 77 were analysed using classical wing morphometrics and 122 bees were successfully analysed using 24 DNA microsatellite markers. A combination of methods including multivariate statistics and assignment tests (frequency-based and Bayesian) revealed the honey bees of this region to resemble the subspecies Apt mellifera macedonica, Apis mellifera carnica or hybrids of these two subspecies. Based on Bayesian assignment ('Structure') and spatial PCA, honey bees within the Serbian territory were composed of 56%-58% A. m. carica and 42%-44% A. m. macedonica. Spatial analysis showed the existence of a north-west to south-east dine in genetic differentiation. The bees in the north-west resemble A. m. carnica, while the bees in the south-east of the country are more similar to A. m. macedonica. Thus, the extent of A. m. macedonica within Serbia was greater than previously estimated. We define a line of hybridisation between A. m. carnica and A. m. macedonica within our study area. The dine of differentiation was still evident using a combination of genetic and spatial information, in spite of beekeeping activities including transhumance and breeding efforts.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Apicultural Research
T1  - Detecting population admixture in honey bees of Serbia
EP  - 313
IS  - 2
SP  - 303
VL  - 53
DO  - 10.3896/IBRA.1.53.2.12
ER  - 

@article{
author = "Nedić, Nebojša and Francis, Roy Mathew and Stanisavljević, Ljubiša and Pihler, Ivan and Kezić, Nikola and Bendixen, Christian and Kryger, Per",
year = "2014",
abstract = "Honey bee workers were sampled across the Serbian territory during 2009-2010 from mostly non-migratory apiaries to determine the population structure of these bees using morphometric, genetic, and spatial information. A total of 134 bees were sampled, of which 77 were analysed using classical wing morphometrics and 122 bees were successfully analysed using 24 DNA microsatellite markers. A combination of methods including multivariate statistics and assignment tests (frequency-based and Bayesian) revealed the honey bees of this region to resemble the subspecies Apt mellifera macedonica, Apis mellifera carnica or hybrids of these two subspecies. Based on Bayesian assignment ('Structure') and spatial PCA, honey bees within the Serbian territory were composed of 56%-58% A. m. carica and 42%-44% A. m. macedonica. Spatial analysis showed the existence of a north-west to south-east dine in genetic differentiation. The bees in the north-west resemble A. m. carnica, while the bees in the south-east of the country are more similar to A. m. macedonica. Thus, the extent of A. m. macedonica within Serbia was greater than previously estimated. We define a line of hybridisation between A. m. carnica and A. m. macedonica within our study area. The dine of differentiation was still evident using a combination of genetic and spatial information, in spite of beekeeping activities including transhumance and breeding efforts.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Apicultural Research",
title = "Detecting population admixture in honey bees of Serbia",
pages = "313-303",
number = "2",
volume = "53",
doi = "10.3896/IBRA.1.53.2.12"
}

Nedić, N., Francis, R. M., Stanisavljević, L., Pihler, I., Kezić, N., Bendixen, C.,& Kryger, P.. (2014). Detecting population admixture in honey bees of Serbia. in Journal of Apicultural Research
Taylor & Francis Ltd, Abingdon., 53(2), 303-313.
https://doi.org/10.3896/IBRA.1.53.2.12

Nedić N, Francis RM, Stanisavljević L, Pihler I, Kezić N, Bendixen C, Kryger P. Detecting population admixture in honey bees of Serbia. in Journal of Apicultural Research. 2014;53(2):303-313.
doi:10.3896/IBRA.1.53.2.12 .

Nedić, Nebojša, Francis, Roy Mathew, Stanisavljević, Ljubiša, Pihler, Ivan, Kezić, Nikola, Bendixen, Christian, Kryger, Per, "Detecting population admixture in honey bees of Serbia" in Journal of Apicultural Research, 53, no. 2 (2014):303-313,
https://doi.org/10.3896/IBRA.1.53.2.12 . .